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The map label for this gene is tufA

Identifier: 28572294

GI number: 28572294

Start: 136972

End: 138165

Strand: Direct

Name: tufA

Synonym: TW131

Alternate gene names: 28572294

Gene position: 136972-138165 (Clockwise)

Preceding gene: 28572293

Following gene: 28572296

Centisome position: 14.79

GC content: 50.5

Gene sequence:

>1194_bases
ATGGCAAAGGCCAAGTTTGAGCGGACTAAACCCCATGTGAATATTGGGACTATAGGTCATGTTGACCACGGTAAAACCAC
ACTGACCGCCGCCATATCGAGGGTTTTGTCCGAGAGGCTTCCTTCGAATACGAATGTAAAGCAGGACTTTGATGCAATTG
ACTCGGCCCCGGAAGAGCGCCAGAGGGGTATTACGATCAATATTTCGCATATCGAGTACGAGACGGAAAAGAGGCACTAC
GCCCATGTTGATGCCCCAGGACATGCCGATTACATAAAGAATATGATTACTGGCGCCGCTCAGATGGATGGTGCGATTCT
TGTTGTTGCGGCGACAGACGGTGCAATGGCCCAGACCCGTGAGCATGTCTTGCTTGCCAAGCAGGTCGGCGTCCCCTATC
TCCTTGTGGCACTCAATAAAGCCGATATGGTGTCTGATGAGGAAATACTCGAGCTTGTTGAGCTTGAGGTGCGCGAGCTG
TTGTCAACTCACGGCTTTGACGGTGAGAATGTTCCCGTTGTACGCGTATCTGGCCTTAAAGCACTTGAAGGTGATCAAAA
GTGGGGCGATGCGGTTATGGAGCTTATGAAAGCCGTTGATGAGAGCATTCCGGACCCCGTTAGAGATAGGGATAAACCCT
TTTTGATGCCCATTGAGGATGTGTTCACAATCACTGGGCGCGGAACGGTTGTTACAGGCCGTGCAGAGCGTGGTGTTCTT
GCGGTTAATTCCGAGGTTGAAATAGTCGGTATACGCCCGACGCAGAAAACCACTGTAACGGGAATTGAAATGTTTCGCAA
GCAGTTGGATGAGGCGTGGGCAGGTGAGAACTGCGGCCTGCTGCTTCGCGGCACAAAACGGGAAGATGTCGAGCGTGGGC
AGGTTGTTGTGAAACCTGGCAGTGTTACACCGCATACAAAGTTTGAGGGAAAGGCGTATATTCTGTCGAAAGATGAGGGT
GGAAGGCATAACCCGTTCTATAGTAACTATCGCCCACAGTTTTACTTCAGGACAACTGACGTGACTGGCGTTATAACCCT
TCCTGAGGGAACAGAAATGGTTATGCCCGGCGATACCATCTCTATAACAGTCGACTTGATTCAGCCAATTGCTATGGAAG
AAGGCTTGGGTTTTGCGATTCGCGAGGGTGGCAGAACTGTTGGCGCAGGCACTGTAGTGAAAATTCTTGAATAG

Upstream 100 bases:

>100_bases
GTCTGTCCGCTTTTGTGTGCTTTTGGCCGTGTTTATGAAATTTGTATTGTAGACTTAGTCCAGCGCGTGTGCGACGGCCG
ACTAGTAGAGGAGATAATCG

Downstream 100 bases:

>100_bases
CTAAGGGTCAAAAGGAATTCAGCTAACAGCTGAGTATAACAGCTGGGCCTTAGTCCAAACGGGTATCCTATCCGGTACTA
TTGCTCCAATACTTCTTGTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAISRVLSERLPSNTNVKQDFDAIDSAPEERQRGITINISHIEYETEKRHY
AHVDAPGHADYIKNMITGAAQMDGAILVVAATDGAMAQTREHVLLAKQVGVPYLLVALNKADMVSDEEILELVELEVREL
LSTHGFDGENVPVVRVSGLKALEGDQKWGDAVMELMKAVDESIPDPVRDRDKPFLMPIEDVFTITGRGTVVTGRAERGVL
AVNSEVEIVGIRPTQKTTVTGIEMFRKQLDEAWAGENCGLLLRGTKREDVERGQVVVKPGSVTPHTKFEGKAYILSKDEG
GRHNPFYSNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTISITVDLIQPIAMEEGLGFAIREGGRTVGAGTVVKILE

Sequences:

>Translated_397_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAISRVLSERLPSNTNVKQDFDAIDSAPEERQRGITINISHIEYETEKRHY
AHVDAPGHADYIKNMITGAAQMDGAILVVAATDGAMAQTREHVLLAKQVGVPYLLVALNKADMVSDEEILELVELEVREL
LSTHGFDGENVPVVRVSGLKALEGDQKWGDAVMELMKAVDESIPDPVRDRDKPFLMPIEDVFTITGRGTVVTGRAERGVL
AVNSEVEIVGIRPTQKTTVTGIEMFRKQLDEAWAGENCGLLLRGTKREDVERGQVVVKPGSVTPHTKFEGKAYILSKDEG
GRHNPFYSNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTISITVDLIQPIAMEEGLGFAIREGGRTVGAGTVVKILE
>Mature_396_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAISRVLSERLPSNTNVKQDFDAIDSAPEERQRGITINISHIEYETEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGAMAQTREHVLLAKQVGVPYLLVALNKADMVSDEEILELVELEVRELL
STHGFDGENVPVVRVSGLKALEGDQKWGDAVMELMKAVDESIPDPVRDRDKPFLMPIEDVFTITGRGTVVTGRAERGVLA
VNSEVEIVGIRPTQKTTVTGIEMFRKQLDEAWAGENCGLLLRGTKREDVERGQVVVKPGSVTPHTKFEGKAYILSKDEGG
RHNPFYSNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTISITVDLIQPIAMEEGLGFAIREGGRTVGAGTVVKILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=55.1637279596977, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI4503475, Length=350, Percent_Identity=35.4285714285714, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=36.1963190184049, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=30.0448430493274, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=30.0448430493274, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=30.9597523219814, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=28.4829721362229, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157426893, Length=286, Percent_Identity=25.8741258741259, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI82546879, Length=430, Percent_Identity=23.2558139534884, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=70.4545454545455, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=70.4545454545455, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=31.0650887573964, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789108, Length=331, Percent_Identity=29.3051359516616, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=29.8755186721992, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788922, Length=138, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=47.2636815920398, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=46.2724935732648, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=329, Percent_Identity=35.8662613981763, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=329, Percent_Identity=35.8662613981763, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=30.1149425287356, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=30.1149425287356, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=318, Percent_Identity=32.7044025157233, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=30.5647840531561, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=26.8292682926829, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=41.4965986394558, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=274, Percent_Identity=28.4671532846715, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=61.8453865336658, Blast_Score=494, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6325337, Length=326, Percent_Identity=35.2760736196319, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=326, Percent_Identity=35.2760736196319, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=341, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=30.0884955752212, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=245, Percent_Identity=27.7551020408163, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=55.0505050505051, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=55.0505050505051, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=50.6393861892583, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=441, Percent_Identity=30.8390022675737, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553816, Length=441, Percent_Identity=30.8390022675737, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24651721, Length=441, Percent_Identity=30.8390022675737, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864154, Length=441, Percent_Identity=30.8390022675737, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24652838, Length=440, Percent_Identity=30.4545454545455, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=440, Percent_Identity=30.4545454545455, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=336, Percent_Identity=31.25, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17137380, Length=320, Percent_Identity=31.25, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI78706572, Length=259, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=34.5238095238095, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_TROW8 (Q83NT9)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789074.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q83NT9
- SMR:   Q83NT9
- STRING:   Q83NT9
- GeneID:   1064426
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW131
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK12736
- BioCyc:   TWHI218496:TW0119-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43508; Mature: 43377

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAISRVLSERLPSNTNVKQDFDAIDSAPEER
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
QRGITINISHIEYETEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGAMAQTR
HCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHH
EHVLLAKQVGVPYLLVALNKADMVSDEEILELVELEVRELLSTHGFDGENVPVVRVSGLK
HHEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCE
ALEGDQKWGDAVMELMKAVDESIPDPVRDRDKPFLMPIEDVFTITGRGTVVTGRAERGVL
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCCCCCEE
AVNSEVEIVGIRPTQKTTVTGIEMFRKQLDEAWAGENCGLLLRGTKREDVERGQVVVKPG
EECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECC
SVTPHTKFEGKAYILSKDEGGRHNPFYSNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTI
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCEE
SITVDLIQPIAMEEGLGFAIREGGRTVGAGTVVKILE
EEEEEHHCHHHHHCCCCEEEECCCCEECCCEEEEEEC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAISRVLSERLPSNTNVKQDFDAIDSAPEER
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
QRGITINISHIEYETEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGAMAQTR
HCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHH
EHVLLAKQVGVPYLLVALNKADMVSDEEILELVELEVRELLSTHGFDGENVPVVRVSGLK
HHEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCE
ALEGDQKWGDAVMELMKAVDESIPDPVRDRDKPFLMPIEDVFTITGRGTVVTGRAERGVL
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCCCCCEE
AVNSEVEIVGIRPTQKTTVTGIEMFRKQLDEAWAGENCGLLLRGTKREDVERGQVVVKPG
EECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECC
SVTPHTKFEGKAYILSKDEGGRHNPFYSNYRPQFYFRTTDVTGVITLPEGTEMVMPGDTI
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCEE
SITVDLIQPIAMEEGLGFAIREGGRTVGAGTVVKILE
EEEEEHHCHHHHHCCCCEEEECCCCEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174