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The map label for this gene is hup [H]

Identifier: 28572291

GI number: 28572291

Start: 132298

End: 132579

Strand: Direct

Name: hup [H]

Synonym: TW128

Alternate gene names: 28572291

Gene position: 132298-132579 (Clockwise)

Preceding gene: 28572290

Following gene: 28572292

Centisome position: 14.29

GC content: 52.13

Gene sequence:

>282_bases
GTGGTTTCTCTGAATCGCACTGAATTGGTTGCTAAGGTTGCTGAGACGTCTGGTGTTAGCCGGGCTACTGTGAACTCTGT
TATTGAGGAGACCTTTGCTGTTTTCGCGGAAACGGTACGTGCCGGCGGTAAGGTGACGATTCCTGGCTGGTGTTCTGTTA
CACGCTCTCATAGAGCTGCCCGTACTGGTCGTAATCCCCAAACCGGTAAGACCGTTGAGATTCCTGCCGGTTATTCTGTT
CGTATCGCTCCCGGCAGCAAGTTGAAAGCCGCAGTTAAGTAG

Upstream 100 bases:

>100_bases
GAGAATTACCCGGTATAACCAAATCAAGCTGGTAAGTTTTTGATTTTGCTGCTAAGATCGCAGTCGGCGTCGCTTCTGGA
CCCAGATATTAGGAGGTAAT

Downstream 100 bases:

>100_bases
ACTGGGGGCACTTATTTCCGCCCCCAATTCATTACAGGGATAAAGGTTTTTGGCGCGTCTTCTCTCTGCCCGTTTCTTTT
CTATTCTTATTGTTTTTTTT

Product: DNA-binding protein HU-alpha

Products: NA

Alternate protein names: HSl [H]

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MVSLNRTELVAKVAETSGVSRATVNSVIEETFAVFAETVRAGGKVTIPGWCSVTRSHRAARTGRNPQTGKTVEIPAGYSV
RIAPGSKLKAAVK

Sequences:

>Translated_93_residues
MVSLNRTELVAKVAETSGVSRATVNSVIEETFAVFAETVRAGGKVTIPGWCSVTRSHRAARTGRNPQTGKTVEIPAGYSV
RIAPGSKLKAAVK
>Mature_93_residues
MVSLNRTELVAKVAETSGVSRATVNSVIEETFAVFAETVRAGGKVTIPGWCSVTRSHRAARTGRNPQTGKTVEIPAGYSV
RIAPGSKLKAAVK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=37.7777777777778, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=34.8314606741573, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9783; Mature: 9783

Theoretical pI: Translated: 11.31; Mature: 11.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLNRTELVAKVAETSGVSRATVNSVIEETFAVFAETVRAGGKVTIPGWCSVTRSHRAA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHH
RTGRNPQTGKTVEIPAGYSVRIAPGSKLKAAVK
HCCCCCCCCCEEECCCCCEEEECCCCCCEECCC
>Mature Secondary Structure
MVSLNRTELVAKVAETSGVSRATVNSVIEETFAVFAETVRAGGKVTIPGWCSVTRSHRAA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHH
RTGRNPQTGKTVEIPAGYSVRIAPGSKLKAAVK
HCCCCCCCCCEEECCCCCEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]