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The map label for this gene is ppnK

Identifier: 28572278

GI number: 28572278

Start: 116812

End: 117729

Strand: Reverse

Name: ppnK

Synonym: TW112

Alternate gene names: 28572278

Gene position: 117729-116812 (Counterclockwise)

Preceding gene: 28572279

Following gene: 28572277

Centisome position: 12.71

GC content: 44.66

Gene sequence:

>918_bases
ATGAGAGTGTATATTGCGCACAATGGTTGTCTTGAAGCTGAGCCTATTTATGGGACTATCTGCGAGCTCGTGGCGCAGAG
AAAAATGTCGGTCATTACAGATCCCCATGCTCGTAATAATGAAAGCGCTCGTAATACTGACAGTGGAGTAGTTTCTCTCA
ATCAAGCCGGGCGCAATAAATATCTGGATCAAGAAACAACATCGCCTGCAACATCACGGTCTATTAATGTGCCGTTTTGT
GCCGGAATATCCATTGGTGGAGATGGCACCTTTCTGCGAATGGCGAGAGACTTGAAAAACACAGGAACCCCGTTATTCGG
AGTAAATATGGGGCGAATGGGATTTCTGGTCGATATCGAACCAGAAGATATAGTGAATCTCGTTGAAAATATCGTAAAAG
GTGAATATACAGAAGAAAAACGACTACCGATAACAGCAAGCGTTCAAAGAGGGGGTAAAAAGATCCACGACGAGTGGGCG
GTAAACGAAATTACAATTGAAAGAAAGGTTGAAGGAAAAGTTGTTGATATAGAGGTATTTGTTGACGGATGCAGGGTGAT
GGACATTAGCTGTAATGGCATTATTATTGCAACCGCAACAGGCTCAACTGCTTATTCGTTTTCTTCGGGTGGACCAATTG
TTTGGCCAGAGATGAAGGTGACTCTGGTTGTTCCGGTTAGCCCGCATGAGCTGTTTGCTAAACCAATCGTGTTGCCAGAT
AATCGATCAATTCTGCTCAAGGTTACATCTAGAGATAATAAAGTAGTGCTGTGCAGTGACGGGCAAGTGAGATTATGCCT
GCAATCCGGCGATGAAATTGCGTGCCACGTGGGAAAGGTGCCTGTTGTGTTTGGCCGTGTGAAAAAGGGGTGTTTTGCAG
AGCATCTCGTAAAGAAATTTAACCTCCAGACCGCTTGA

Upstream 100 bases:

>100_bases
TCTGATCCCGTGGATAAAAACCCTACCCCTGTGTCCTGCTGACTCTGGTAACTCTGATAAATCCTCCAAATAACCACACT
TGGTAAGAGACAGTGTGTAA

Downstream 100 bases:

>100_bases
GGGTCGGCGCTGTGCAGAATGATTGAACAAATCTCTATACGCAATTTTGGAAATATAGCAAAAGCAGATATAGATTTTGG
TTCCGATTTTACCGTGATAA

Product: ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRVYIAHNGCLEAEPIYGTICELVAQRKMSVITDPHARNNESARNTDSGVVSLNQAGRNKYLDQETTSPATSRSINVPFC
AGISIGGDGTFLRMARDLKNTGTPLFGVNMGRMGFLVDIEPEDIVNLVENIVKGEYTEEKRLPITASVQRGGKKIHDEWA
VNEITIERKVEGKVVDIEVFVDGCRVMDISCNGIIIATATGSTAYSFSSGGPIVWPEMKVTLVVPVSPHELFAKPIVLPD
NRSILLKVTSRDNKVVLCSDGQVRLCLQSGDEIACHVGKVPVVFGRVKKGCFAEHLVKKFNLQTA

Sequences:

>Translated_305_residues
MRVYIAHNGCLEAEPIYGTICELVAQRKMSVITDPHARNNESARNTDSGVVSLNQAGRNKYLDQETTSPATSRSINVPFC
AGISIGGDGTFLRMARDLKNTGTPLFGVNMGRMGFLVDIEPEDIVNLVENIVKGEYTEEKRLPITASVQRGGKKIHDEWA
VNEITIERKVEGKVVDIEVFVDGCRVMDISCNGIIIATATGSTAYSFSSGGPIVWPEMKVTLVVPVSPHELFAKPIVLPD
NRSILLKVTSRDNKVVLCSDGQVRLCLQSGDEIACHVGKVPVVFGRVKKGCFAEHLVKKFNLQTA
>Mature_305_residues
MRVYIAHNGCLEAEPIYGTICELVAQRKMSVITDPHARNNESARNTDSGVVSLNQAGRNKYLDQETTSPATSRSINVPFC
AGISIGGDGTFLRMARDLKNTGTPLFGVNMGRMGFLVDIEPEDIVNLVENIVKGEYTEEKRLPITASVQRGGKKIHDEWA
VNEITIERKVEGKVVDIEVFVDGCRVMDISCNGIIIATATGSTAYSFSSGGPIVWPEMKVTLVVPVSPHELFAKPIVLPD
NRSILLKVTSRDNKVVLCSDGQVRLCLQSGDEIACHVGKVPVVFGRVKKGCFAEHLVKKFNLQTA

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788968, Length=192, Percent_Identity=32.8125, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=217, Percent_Identity=29.9539170506912, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320794, Length=240, Percent_Identity=27.5, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325068, Length=200, Percent_Identity=29.5, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573830, Length=219, Percent_Identity=24.2009132420091, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573828, Length=219, Percent_Identity=24.2009132420091, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573832, Length=219, Percent_Identity=24.2009132420091, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161077047, Length=219, Percent_Identity=24.2009132420091, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28573826, Length=219, Percent_Identity=24.2009132420091, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_TROW8 (Q83IC3)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789058.1
- ProteinModelPortal:   Q83IC3
- SMR:   Q83IC3
- STRING:   Q83IC3
- GeneID:   1064410
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW112
- eggNOG:   COG0061
- HOGENOM:   HBG251158
- OMA:   CVPEGFD
- PhylomeDB:   Q83IC3
- ProtClustDB:   CLSK229483
- BioCyc:   TWHI218496:TW0103-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33274; Mature: 33274

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYIAHNGCLEAEPIYGTICELVAQRKMSVITDPHARNNESARNTDSGVVSLNQAGRNK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCCCC
YLDQETTSPATSRSINVPFCAGISIGGDGTFLRMARDLKNTGTPLFGVNMGRMGFLVDIE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEC
PEDIVNLVENIVKGEYTEEKRLPITASVQRGGKKIHDEWAVNEITIERKVEGKVVDIEVF
HHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCCCCCEEEEEEEEECCCEEEEEEEE
VDGCRVMDISCNGIIIATATGSTAYSFSSGGPIVWPEMKVTLVVPVSPHELFAKPIVLPD
ECCEEEEEEECCEEEEEEECCCCEEEECCCCCEEECCEEEEEEEECCHHHHHHCCEEECC
NRSILLKVTSRDNKVVLCSDGQVRLCLQSGDEIACHVGKVPVVFGRVKKGCFAEHLVKKF
CCEEEEEEECCCCEEEEECCCCEEEEEECCCEEEEEECCCCEEEEHHHCCHHHHHHHHHH
NLQTA
CCCCC
>Mature Secondary Structure
MRVYIAHNGCLEAEPIYGTICELVAQRKMSVITDPHARNNESARNTDSGVVSLNQAGRNK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEECCCCCCC
YLDQETTSPATSRSINVPFCAGISIGGDGTFLRMARDLKNTGTPLFGVNMGRMGFLVDIE
CCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEC
PEDIVNLVENIVKGEYTEEKRLPITASVQRGGKKIHDEWAVNEITIERKVEGKVVDIEVF
HHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCCCCCEEEEEEEEECCCEEEEEEEE
VDGCRVMDISCNGIIIATATGSTAYSFSSGGPIVWPEMKVTLVVPVSPHELFAKPIVLPD
ECCEEEEEEECCEEEEEEECCCCEEEECCCCCEEECCEEEEEEEECCHHHHHHCCEEECC
NRSILLKVTSRDNKVVLCSDGQVRLCLQSGDEIACHVGKVPVVFGRVKKGCFAEHLVKKF
CCEEEEEEECCCCEEEEECCCCEEEEEECCCEEEEEECCCCEEEEHHHCCHHHHHHHHHH
NLQTA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174