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The map label for this gene is pyrG

Identifier: 28572276

GI number: 28572276

Start: 111707

End: 113344

Strand: Reverse

Name: pyrG

Synonym: TW110

Alternate gene names: 28572276

Gene position: 113344-111707 (Counterclockwise)

Preceding gene: 28572277

Following gene: 28572275

Centisome position: 12.24

GC content: 47.5

Gene sequence:

>1638_bases
GTGAACGGCATAAAACATATATTCATAACAGGTGGGGTTGTTTCCTCAATAGGTAAGGGGCTAACAGCAGCCAGCTTGGG
CAGTCTGCTCACAATGCGTGGGCTAAAGGTGTTTATAAAAAAGCTGGATCCGTATCTGAATGTTGATCCTGGCACGATGA
ATCCGCTGCAGCATGGTGAAGTGTTTATAACCGAGGATGGCGCAGAAACCGATCTTGATGTTGGGCACTACGAACGGTTC
TTGGATGTGGATCTCGATAGAACAGCGAATGTTACAACGGGGCAAGTGTACTCCAAGGTAATCACTCTTGAAAGAACCGG
CAGATATCTTGGCGAAACTGTACAGGTTATACCTCATATAACTGATGAGATAAAGCGCAGGATACGACTCGCACCTGATG
ATATTGATGTCATAATAACCGAAATAGGGGGCACGGTTGGGGATATTGAGTCCCAGCCATTTATCGAGGCGGCCAGGCAG
ATAAGGCGCGAAGTTGGCAGAGAAAACTGCTGTTTTATACATGTATCACTTGTCCCCTTTCTCGAGAGTGCGGGTGAGCA
GAAAACAAAACCCACTCAGCATTCGGTTGCTATATTGAGAGCCGCGGGAATTCAACCCGATGCCCTTGTCTTGCGGAGCG
ATAAGCCCATTTCAAGTGCCAATCAGAAAAAGATAGCACTTATGTGTGATGTCAGTGCTGTTGTAAACGCGGTCACTGTT
CCAAACATCTATGAAATACCTTGTGTTCTAAATCAACACGGGTTAGACAAGTTTGTAATCAAGCACCTCGACCTTGAGGC
GGGTGAAATTGACTGGTCCTATTGGGACGATGTGCTTGATGCGATAGAGAACAATAGAAGTGAAATAGAGATAGCCATTA
TTGGAAAGTATACGGGACTGCCCGACGCATACATTTCTGTTATTGAGGCGCTTAGGGCTGGCGGATTCGAGAGTAAGACA
AAGGTTAATATAAAGTGGGTTGACTCAGATGATGAGAACCTTGAGGATCAGCTGGAAAACATCAACGGCGCTTGTATCCC
CGGCGGTTTTGGAATACGGGGCATAGAAGGACTTATAAAAGCCATACGGATTTGCCGCGAAAGAGAAATTCCTACTCTCG
GTATATGTCTCGGGATGCAGTGCATGGTGATAGAGTATGCCAGACATGTTGTTGGGATGCATGGCGCATCGTCAACGGAG
TTTACCGATGATACTCAGTGGCCAGTTGTAACAACAATGCTTGAACAAAGAGATATTCTTATTGACGATCAGTTTGGCGG
GACAATGCGCCTGGGAAGCTACCGTGCCGTTCTGAGTGTCGGGAGTCTAGCTGCCAGTTTATATGGCACAACTGATATAC
ACGAGCGGCATAGGCACCGCTATGAGGTAAATAATGGCTATGCACAGCGACTGGTTGAGCGTGGGCTTATAGTCTCTGGG
CGGAATACAGAGCAAGACCTGATTGAATTTATTGAGCTCGACCGCAAGGATCACCCGTTCTACATTGGCACACAGGCGCA
TCCGGAATGTAAGTCGCGACCAAAGAGGCCACACCCGCTGTTCAAGGGCCTTGTGGCAGCGGCTTTAGCTAGGAAAGAAA
TAGCGGAATTCAATTCCGGCAAGACGGTGCTTCAATAG

Upstream 100 bases:

>100_bases
ATAGACTGGATATGCACAAAGCAGAACACAGGTCCAATCTGACTATTTTTCAGCATGCGTTATTAGTAGACAATAAAATG
CAATACAATGAAACCCTGTA

Downstream 100 bases:

>100_bases
TAACCGTCCGTGCATCATTTGTTCCTATATCACCTATTTCCCTATATCACTTATTTCATTGTTGACAAGCTTAGGTTATA
CCAGCTACCCAACTGTTTAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNGIKHIFITGGVVSSIGKGLTAASLGSLLTMRGLKVFIKKLDPYLNVDPGTMNPLQHGEVFITEDGAETDLDVGHYERF
LDVDLDRTANVTTGQVYSKVITLERTGRYLGETVQVIPHITDEIKRRIRLAPDDIDVIITEIGGTVGDIESQPFIEAARQ
IRREVGRENCCFIHVSLVPFLESAGEQKTKPTQHSVAILRAAGIQPDALVLRSDKPISSANQKKIALMCDVSAVVNAVTV
PNIYEIPCVLNQHGLDKFVIKHLDLEAGEIDWSYWDDVLDAIENNRSEIEIAIIGKYTGLPDAYISVIEALRAGGFESKT
KVNIKWVDSDDENLEDQLENINGACIPGGFGIRGIEGLIKAIRICREREIPTLGICLGMQCMVIEYARHVVGMHGASSTE
FTDDTQWPVVTTMLEQRDILIDDQFGGTMRLGSYRAVLSVGSLAASLYGTTDIHERHRHRYEVNNGYAQRLVERGLIVSG
RNTEQDLIEFIELDRKDHPFYIGTQAHPECKSRPKRPHPLFKGLVAAALARKEIAEFNSGKTVLQ

Sequences:

>Translated_545_residues
MNGIKHIFITGGVVSSIGKGLTAASLGSLLTMRGLKVFIKKLDPYLNVDPGTMNPLQHGEVFITEDGAETDLDVGHYERF
LDVDLDRTANVTTGQVYSKVITLERTGRYLGETVQVIPHITDEIKRRIRLAPDDIDVIITEIGGTVGDIESQPFIEAARQ
IRREVGRENCCFIHVSLVPFLESAGEQKTKPTQHSVAILRAAGIQPDALVLRSDKPISSANQKKIALMCDVSAVVNAVTV
PNIYEIPCVLNQHGLDKFVIKHLDLEAGEIDWSYWDDVLDAIENNRSEIEIAIIGKYTGLPDAYISVIEALRAGGFESKT
KVNIKWVDSDDENLEDQLENINGACIPGGFGIRGIEGLIKAIRICREREIPTLGICLGMQCMVIEYARHVVGMHGASSTE
FTDDTQWPVVTTMLEQRDILIDDQFGGTMRLGSYRAVLSVGSLAASLYGTTDIHERHRHRYEVNNGYAQRLVERGLIVSG
RNTEQDLIEFIELDRKDHPFYIGTQAHPECKSRPKRPHPLFKGLVAAALARKEIAEFNSGKTVLQ
>Mature_545_residues
MNGIKHIFITGGVVSSIGKGLTAASLGSLLTMRGLKVFIKKLDPYLNVDPGTMNPLQHGEVFITEDGAETDLDVGHYERF
LDVDLDRTANVTTGQVYSKVITLERTGRYLGETVQVIPHITDEIKRRIRLAPDDIDVIITEIGGTVGDIESQPFIEAARQ
IRREVGRENCCFIHVSLVPFLESAGEQKTKPTQHSVAILRAAGIQPDALVLRSDKPISSANQKKIALMCDVSAVVNAVTV
PNIYEIPCVLNQHGLDKFVIKHLDLEAGEIDWSYWDDVLDAIENNRSEIEIAIIGKYTGLPDAYISVIEALRAGGFESKT
KVNIKWVDSDDENLEDQLENINGACIPGGFGIRGIEGLIKAIRICREREIPTLGICLGMQCMVIEYARHVVGMHGASSTE
FTDDTQWPVVTTMLEQRDILIDDQFGGTMRLGSYRAVLSVGSLAASLYGTTDIHERHRHRYEVNNGYAQRLVERGLIVSG
RNTEQDLIEFIELDRKDHPFYIGTQAHPECKSRPKRPHPLFKGLVAAALARKEIAEFNSGKTVLQ

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=556, Percent_Identity=44.9640287769784, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI28559085, Length=553, Percent_Identity=42.49547920434, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI28559083, Length=553, Percent_Identity=42.49547920434, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI221316689, Length=553, Percent_Identity=42.49547920434, Blast_Score=431, Evalue=1e-121,
Organism=Escherichia coli, GI1789142, Length=534, Percent_Identity=51.310861423221, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI25148299, Length=621, Percent_Identity=37.3590982286634, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=42.7320490367776, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=42.5044091710758, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=43.8282647584973, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=42.9423459244533, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_TROW8 (Q83IC4)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789056.1
- HSSP:   P17812
- ProteinModelPortal:   Q83IC4
- SMR:   Q83IC4
- STRING:   Q83IC4
- GeneID:   1064408
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW110
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   TWHI218496:TW0101-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60364; Mature: 60364

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 376-376 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIKHIFITGGVVSSIGKGLTAASLGSLLTMRGLKVFIKKLDPYLNVDPGTMNPLQHGE
CCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
VFITEDGAETDLDVGHYERFLDVDLDRTANVTTGQVYSKVITLERTGRYLGETVQVIPHI
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
TDEIKRRIRLAPDDIDVIITEIGGTVGDIESQPFIEAARQIRREVGRENCCFIHVSLVPF
HHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LESAGEQKTKPTQHSVAILRAAGIQPDALVLRSDKPISSANQKKIALMCDVSAVVNAVTV
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHCC
PNIYEIPCVLNQHGLDKFVIKHLDLEAGEIDWSYWDDVLDAIENNRSEIEIAIIGKYTGL
CCCEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
PDAYISVIEALRAGGFESKTKVNIKWVDSDDENLEDQLENINGACIPGGFGIRGIEGLIK
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHH
AIRICREREIPTLGICLGMQCMVIEYARHVVGMHGASSTEFTDDTQWPVVTTMLEQRDIL
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCE
IDDQFGGTMRLGSYRAVLSVGSLAASLYGTTDIHERHRHRYEVNNGYAQRLVERGLIVSG
EECCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCHHHHHHHCCCEEEC
RNTEQDLIEFIELDRKDHPFYIGTQAHPECKSRPKRPHPLFKGLVAAALARKEIAEFNSG
CCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KTVLQ
CCCCC
>Mature Secondary Structure
MNGIKHIFITGGVVSSIGKGLTAASLGSLLTMRGLKVFIKKLDPYLNVDPGTMNPLQHGE
CCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
VFITEDGAETDLDVGHYERFLDVDLDRTANVTTGQVYSKVITLERTGRYLGETVQVIPHI
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
TDEIKRRIRLAPDDIDVIITEIGGTVGDIESQPFIEAARQIRREVGRENCCFIHVSLVPF
HHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LESAGEQKTKPTQHSVAILRAAGIQPDALVLRSDKPISSANQKKIALMCDVSAVVNAVTV
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHCC
PNIYEIPCVLNQHGLDKFVIKHLDLEAGEIDWSYWDDVLDAIENNRSEIEIAIIGKYTGL
CCCEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
PDAYISVIEALRAGGFESKTKVNIKWVDSDDENLEDQLENINGACIPGGFGIRGIEGLIK
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHCCCCEEECCCCCHHHHHHHHH
AIRICREREIPTLGICLGMQCMVIEYARHVVGMHGASSTEFTDDTQWPVVTTMLEQRDIL
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCE
IDDQFGGTMRLGSYRAVLSVGSLAASLYGTTDIHERHRHRYEVNNGYAQRLVERGLIVSG
EECCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCHHHHHHHCCCEEEC
RNTEQDLIEFIELDRKDHPFYIGTQAHPECKSRPKRPHPLFKGLVAAALARKEIAEFNSG
CCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KTVLQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174