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The map label for this gene is truB

Identifier: 28572264

GI number: 28572264

Start: 96582

End: 97733

Strand: Reverse

Name: truB

Synonym: TW097

Alternate gene names: 28572264

Gene position: 97733-96582 (Counterclockwise)

Preceding gene: 28572266

Following gene: 28572262

Centisome position: 10.56

GC content: 44.44

Gene sequence:

>1152_bases
ATGTTAGGTAAAGTTGAACGTTCTGAGATGTATATACTGTTCGCTATGACGCAGGTTCTTCTGGTAGACAAAATAAGCGG
CATTACAAGTCACACAGCTGTTGCAAAAATAAGGCACCTCACAGGAGTAAAAAAAATCGGGCATTGCGGAACCCTCGATC
CGGCAGCGTGTGGTCTTTTGATAATGGGATGCGGAACTGCAACAAGACTTATAAGATATATGTCCAATCTTGATAAGCGG
TATATTGCAACAATAACGCTGGGCACACAAACAACAACTGATGATTCAGAAGGTGAGATTATATACAGTGCACCAAAGCC
ATCCCTTGACAAAATTACACTCGAGAGCATAGGGAGGGCTGCGGAGAAATTAAGCGGAACAATCAAACAAATCCCGAGCG
CATACAGCGCAATAAAGGTCTCTGGAAATAGAGCGTACAACCTAGCGCGTCAGGGGATTATCCCAAAGCTCAATGCACGA
GAGGTGCGGGTGCATTGGAAATTTCTCGGAGATTTTGAAAATAACCAAGTTCATGTGCAGATTACCTGTTCATCTGGTAC
ATACGTTAGGGCATTGGCCCGGGATATGGGAAAATTCCTGGGTGTTGGCGGGCACTTATCATACCTAAAACGCCTCAGCA
TAGGGCCATTTCATTTGCATGAAATATATCGGGAAATAAACAAAAAGGAAGCCACCATGTCTGAGCGAACGCCTAGTGGT
AATACGCAAGGATTAACTGACAACATGGCCATCTCCGACAACATGGCCATCTCCGAGAGTGATAAGCACGATTGTACTGA
GCCTGGAATTAATTGTACTGAGCTTGGAATTAAAGATACATGCACAGCGCTTCGTGAAGTGCACTATACACAAGGTGATA
CACTGTCATTCACTCGCCTCACAGCCCTCCAGGCATTATCAAGAATTTACAAACCGATAGAAGTTAGTCAAAAACAGGCA
GATGACCTAAGTTGCGGGCGATATATATCTCTTGGAATCGACTCAAACGGCCCGGTGTGTGCAGTTTGTAAAGAAAATTT
AATTGCGGTTATTCAGCCGGTATCAGCAGGATTATGGCGCCCTGAAACAGTTCTCAGTGATAACAGAAAACTCAACAGTA
ATGCGGCACAAGACGCAAGTGGAAGCACATAA

Upstream 100 bases:

>100_bases
GCAACTGCAATATACACAAAAATAAGAATGATCAGGACACCAAGAAAAAACATCAAACCCCCTTCTTGATCGAGGAAATG
ATACCAGACTCATTTGGCCT

Downstream 100 bases:

>100_bases
AACCCAAATCCATCGAGAAAAAGCCCGCCGAATAGTGCACCCGAACCTATCCCGATGTTGAAACTCATAGTGTAATAAGC
GTTGGCGATATCACGATTAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MLGKVERSEMYILFAMTQVLLVDKISGITSHTAVAKIRHLTGVKKIGHCGTLDPAACGLLIMGCGTATRLIRYMSNLDKR
YIATITLGTQTTTDDSEGEIIYSAPKPSLDKITLESIGRAAEKLSGTIKQIPSAYSAIKVSGNRAYNLARQGIIPKLNAR
EVRVHWKFLGDFENNQVHVQITCSSGTYVRALARDMGKFLGVGGHLSYLKRLSIGPFHLHEIYREINKKEATMSERTPSG
NTQGLTDNMAISDNMAISESDKHDCTEPGINCTELGIKDTCTALREVHYTQGDTLSFTRLTALQALSRIYKPIEVSQKQA
DDLSCGRYISLGIDSNGPVCAVCKENLIAVIQPVSAGLWRPETVLSDNRKLNSNAAQDASGST

Sequences:

>Translated_383_residues
MLGKVERSEMYILFAMTQVLLVDKISGITSHTAVAKIRHLTGVKKIGHCGTLDPAACGLLIMGCGTATRLIRYMSNLDKR
YIATITLGTQTTTDDSEGEIIYSAPKPSLDKITLESIGRAAEKLSGTIKQIPSAYSAIKVSGNRAYNLARQGIIPKLNAR
EVRVHWKFLGDFENNQVHVQITCSSGTYVRALARDMGKFLGVGGHLSYLKRLSIGPFHLHEIYREINKKEATMSERTPSG
NTQGLTDNMAISDNMAISESDKHDCTEPGINCTELGIKDTCTALREVHYTQGDTLSFTRLTALQALSRIYKPIEVSQKQA
DDLSCGRYISLGIDSNGPVCAVCKENLIAVIQPVSAGLWRPETVLSDNRKLNSNAAQDASGST
>Mature_383_residues
MLGKVERSEMYILFAMTQVLLVDKISGITSHTAVAKIRHLTGVKKIGHCGTLDPAACGLLIMGCGTATRLIRYMSNLDKR
YIATITLGTQTTTDDSEGEIIYSAPKPSLDKITLESIGRAAEKLSGTIKQIPSAYSAIKVSGNRAYNLARQGIIPKLNAR
EVRVHWKFLGDFENNQVHVQITCSSGTYVRALARDMGKFLGVGGHLSYLKRLSIGPFHLHEIYREINKKEATMSERTPSG
NTQGLTDNMAISDNMAISESDKHDCTEPGINCTELGIKDTCTALREVHYTQGDTLSFTRLTALQALSRIYKPIEVSQKQA
DDLSCGRYISLGIDSNGPVCAVCKENLIAVIQPVSAGLWRPETVLSDNRKLNSNAAQDASGST

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=178, Percent_Identity=38.7640449438202, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI2367200, Length=226, Percent_Identity=34.9557522123894, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323204, Length=199, Percent_Identity=31.1557788944724, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=35.3383458646617, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=27.2277227722772, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_TROW8 (Q820Z5)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789044.1
- ProteinModelPortal:   Q820Z5
- SMR:   Q820Z5
- STRING:   Q820Z5
- GeneID:   1064327
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW097
- eggNOG:   COG0130
- HOGENOM:   HBG251206
- OMA:   MAISESD
- ProtClustDB:   CLSK2516679
- BioCyc:   TWHI218496:TW0089-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 41740; Mature: 41740

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKVERSEMYILFAMTQVLLVDKISGITSHTAVAKIRHLTGVKKIGHCGTLDPAACGLL
CCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCEE
IMGCGTATRLIRYMSNLDKRYIATITLGTQTTTDDSEGEIIYSAPKPSLDKITLESIGRA
EECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHEEHHHHHHH
AEKLSGTIKQIPSAYSAIKVSGNRAYNLARQGIIPKLNAREVRVHWKFLGDFENNQVHVQ
HHHHHHHHHHCCCHHHEEEECCCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEE
ITCSSGTYVRALARDMGKFLGVGGHLSYLKRLSIGPFHLHEIYREINKKEATMSERTPSG
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NTQGLTDNMAISDNMAISESDKHDCTEPGINCTELGIKDTCTALREVHYTQGDTLSFTRL
CCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
TALQALSRIYKPIEVSQKQADDLSCGRYISLGIDSNGPVCAVCKENLIAVIQPVSAGLWR
HHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEHHCCEEEEEHHHCCCCC
PETVLSDNRKLNSNAAQDASGST
CHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLGKVERSEMYILFAMTQVLLVDKISGITSHTAVAKIRHLTGVKKIGHCGTLDPAACGLL
CCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCEE
IMGCGTATRLIRYMSNLDKRYIATITLGTQTTTDDSEGEIIYSAPKPSLDKITLESIGRA
EECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHEEHHHHHHH
AEKLSGTIKQIPSAYSAIKVSGNRAYNLARQGIIPKLNAREVRVHWKFLGDFENNQVHVQ
HHHHHHHHHHCCCHHHEEEECCCHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEE
ITCSSGTYVRALARDMGKFLGVGGHLSYLKRLSIGPFHLHEIYREINKKEATMSERTPSG
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NTQGLTDNMAISDNMAISESDKHDCTEPGINCTELGIKDTCTALREVHYTQGDTLSFTRL
CCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
TALQALSRIYKPIEVSQKQADDLSCGRYISLGIDSNGPVCAVCKENLIAVIQPVSAGLWR
HHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEHHCCEEEEEHHHCCCCC
PETVLSDNRKLNSNAAQDASGST
CHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174