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The map label for this gene is purH [H]

Identifier: 28572261

GI number: 28572261

Start: 93477

End: 95105

Strand: Direct

Name: purH [H]

Synonym: TW094

Alternate gene names: 28572261

Gene position: 93477-95105 (Clockwise)

Preceding gene: 28572260

Following gene: 28572263

Centisome position: 10.1

GC content: 47.08

Gene sequence:

>1629_bases
ATGACGACAAATATACGTATAAAAAGAGCTCTTATTTCAGTGAGTGATAAGTCAGGCCTTGCAGATCTAGCAGAAGCCCT
CGCGGCGCATTCTGTCAAAATAGTGTCAACCGGCAGCACGGCAGAGTTCATACGCGGGGTGTCTATTCCCGTGCGCGATG
TATCTGAGGTAACAGGGGTTGGGGAACTCTTGGATGGCCGCGTGAAAACCCTGCATCCCAAAATACATGCCCCGATTCTT
GCTGACACAACTTCACAGATGCACCGCGCGCAGCTACAGCAGCTTGGCGTTGATGCTTTTGATCTTGTCGTGGTCAACCT
GTATCCATTTTTTGAAATTTCCAAAAACTCTGAAGCAGAATTCTCTGATGTAATTGAGCAAATTGACATTGGTGGCTCTG
CCCTAATAAGGGCTGCGGCAAAAAATCATACACGAGTTGTTGTTATTGTTGATCCAAGTGACTACATTCATGTGATTAAC
AGCCTTGAAAGGGGTGCCCCGTCCCGCCTGCGCCACCAACTCGCTATAAAGGCGTATTCACACACTTCCGAATATGATCT
TCATATTTCCAGATGGCTTTCCGAGCGGTTTTACAAACATACTCTTTATTCTTCGAGTGATTCTGTAGGTGATGTTTGTG
ATAACAATCTCGATTCCAATGATCATTTTGTTGAACTGCGTGGCAAAAAACTGGGTGATCTGCGCTACGGCGAAAACAGT
CATCAGAAGGCTTCCCTATACCTGTCTTGCAAGGAGAGCGTTCCCCAATTGGGCTTGGCGAGTGCAGCTCTTCTTGGGGG
CAAGCAGATGTCGTACAACAATTATCTTGACGCAGATGTTGCCTCAAGAATTGTCAACAGCTTTGATCTGCCAACAGTAT
CGTTTGTAAAGCACGGTAACCCTTGTGGAATTGCCTCAAATATTGAGGTTGCTATTGCATGCAGAAATGCTCATGAGTGT
GACCCAACTTCAGCCTTTGGCGGTGTTGTCGCTGTCAATAGGGAAGTTACCCTTGACGTTGCAACTCATCTTTTGCCGAT
TTTTATAGAGGTAGTTGTGGCGCCCGGTTATGACCCACAAGCACTACAGCTTTTGCTAAAAAAGAAGAATCTGAGGGTTG
TAGAGCTTCCTGAGTGCGCTTCGTATCCGCAAGATCAGTTGCGGCAAATCTCCGGTGGATTTCTGGTGCAGGACGCAGAC
AAATTTGACCATCACGACACATTTTCCAGCTGGACTCAGGTGTCTGGCCCACGTATTTTTATGGATGCCGGAGCTGATAA
CGAGACGCTAATGGATTTGGAATTTGCCTGGAAATCCTGTGCGTTTGTTAAATCCAATGCGATTCTTCTGGCAAAAAACA
TGGCATCGGTTGGAATAGGAATGGGGCAGGTAAATAGACTAGATGCGTGTTACCTGGCTGTAAATCGTGCGGGATCCAGA
TCCCTGGGTGCAGTCGCCGCATCTGATGCATTTTTTCCCTTTTCTGATGGCCTGGATGTGCTAATAAATTCCGGAGTAAA
GGCTATTGTGCAGCCGGGCGGGTCTGTTCGTGATAATGCTGTTATAGCTGCTGCTCAACAGGCGGGTGTCACCATGTTTT
TTACCGGCAAGAGGCATTTTGCGCATTGA

Upstream 100 bases:

>100_bases
TCTAAAGAATCTTTTGCATAACAGCGTGAGGCGTCCCTCTGGTAAAACAGGAGATCGATGAACGGGTCGGTAAATTTTAG
TGATAAGTAATGTTTAGAAT

Downstream 100 bases:

>100_bases
ATGTTTTGCCCCACAGTGTTTCTCTGGACCATTTCTGAAGACAAAAACATGCAACGTGCCCCTTATTTAGTAGCTTAATC
AAGCAAGACAAGCACAAAAC

Product: bifunctional purine biosynthesis protein PurH

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTTNIRIKRALISVSDKSGLADLAEALAAHSVKIVSTGSTAEFIRGVSIPVRDVSEVTGVGELLDGRVKTLHPKIHAPIL
ADTTSQMHRAQLQQLGVDAFDLVVVNLYPFFEISKNSEAEFSDVIEQIDIGGSALIRAAAKNHTRVVVIVDPSDYIHVIN
SLERGAPSRLRHQLAIKAYSHTSEYDLHISRWLSERFYKHTLYSSSDSVGDVCDNNLDSNDHFVELRGKKLGDLRYGENS
HQKASLYLSCKESVPQLGLASAALLGGKQMSYNNYLDADVASRIVNSFDLPTVSFVKHGNPCGIASNIEVAIACRNAHEC
DPTSAFGGVVAVNREVTLDVATHLLPIFIEVVVAPGYDPQALQLLLKKKNLRVVELPECASYPQDQLRQISGGFLVQDAD
KFDHHDTFSSWTQVSGPRIFMDAGADNETLMDLEFAWKSCAFVKSNAILLAKNMASVGIGMGQVNRLDACYLAVNRAGSR
SLGAVAASDAFFPFSDGLDVLINSGVKAIVQPGGSVRDNAVIAAAQQAGVTMFFTGKRHFAH

Sequences:

>Translated_542_residues
MTTNIRIKRALISVSDKSGLADLAEALAAHSVKIVSTGSTAEFIRGVSIPVRDVSEVTGVGELLDGRVKTLHPKIHAPIL
ADTTSQMHRAQLQQLGVDAFDLVVVNLYPFFEISKNSEAEFSDVIEQIDIGGSALIRAAAKNHTRVVVIVDPSDYIHVIN
SLERGAPSRLRHQLAIKAYSHTSEYDLHISRWLSERFYKHTLYSSSDSVGDVCDNNLDSNDHFVELRGKKLGDLRYGENS
HQKASLYLSCKESVPQLGLASAALLGGKQMSYNNYLDADVASRIVNSFDLPTVSFVKHGNPCGIASNIEVAIACRNAHEC
DPTSAFGGVVAVNREVTLDVATHLLPIFIEVVVAPGYDPQALQLLLKKKNLRVVELPECASYPQDQLRQISGGFLVQDAD
KFDHHDTFSSWTQVSGPRIFMDAGADNETLMDLEFAWKSCAFVKSNAILLAKNMASVGIGMGQVNRLDACYLAVNRAGSR
SLGAVAASDAFFPFSDGLDVLINSGVKAIVQPGGSVRDNAVIAAAQQAGVTMFFTGKRHFAH
>Mature_541_residues
TTNIRIKRALISVSDKSGLADLAEALAAHSVKIVSTGSTAEFIRGVSIPVRDVSEVTGVGELLDGRVKTLHPKIHAPILA
DTTSQMHRAQLQQLGVDAFDLVVVNLYPFFEISKNSEAEFSDVIEQIDIGGSALIRAAAKNHTRVVVIVDPSDYIHVINS
LERGAPSRLRHQLAIKAYSHTSEYDLHISRWLSERFYKHTLYSSSDSVGDVCDNNLDSNDHFVELRGKKLGDLRYGENSH
QKASLYLSCKESVPQLGLASAALLGGKQMSYNNYLDADVASRIVNSFDLPTVSFVKHGNPCGIASNIEVAIACRNAHECD
PTSAFGGVVAVNREVTLDVATHLLPIFIEVVVAPGYDPQALQLLLKKKNLRVVELPECASYPQDQLRQISGGFLVQDADK
FDHHDTFSSWTQVSGPRIFMDAGADNETLMDLEFAWKSCAFVKSNAILLAKNMASVGIGMGQVNRLDACYLAVNRAGSRS
LGAVAASDAFFPFSDGLDVLINSGVKAIVQPGGSVRDNAVIAAAQQAGVTMFFTGKRHFAH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=503, Percent_Identity=30.8151093439364, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1790439, Length=544, Percent_Identity=39.5220588235294, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71985564, Length=504, Percent_Identity=31.9444444444444, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6323056, Length=498, Percent_Identity=32.5301204819277, Blast_Score=217, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6323768, Length=499, Percent_Identity=32.2645290581162, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24649832, Length=504, Percent_Identity=32.3412698412698, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 58722; Mature: 58591

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNIRIKRALISVSDKSGLADLAEALAAHSVKIVSTGSTAEFIRGVSIPVRDVSEVTGV
CCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHHHHCH
GELLDGRVKTLHPKIHAPILADTTSQMHRAQLQQLGVDAFDLVVVNLYPFFEISKNSEAE
HHHHCCCCEEECCCCCCCEEECCHHHHHHHHHHHCCCCHHHEEEEEEEEEEEECCCCCCH
FSDVIEQIDIGGSALIRAAAKNHTRVVVIVDPSDYIHVINSLERGAPSRLRHQLAIKAYS
HHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHEEEEC
HTSEYDLHISRWLSERFYKHTLYSSSDSVGDVCDNNLDSNDHFVELRGKKLGDLRYGENS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
HQKASLYLSCKESVPQLGLASAALLGGKQMSYNNYLDADVASRIVNSFDLPTVSFVKHGN
CCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCCCC
PCGIASNIEVAIACRNAHECDPTSAFGGVVAVNREVTLDVATHLLPIFIEVVVAPGYDPQ
CCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEEHHHHHHHHHHHHHHEECCCCCHH
ALQLLLKKKNLRVVELPECASYPQDQLRQISGGFLVQDADKFDHHDTFSSWTQVSGPRIF
HHHHHHHHCCCEEEECCHHHCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCCCEEE
MDAGADNETLMDLEFAWKSCAFVKSNAILLAKNMASVGIGMGQVNRLDACYLAVNRAGSR
EECCCCCCEEEEHHHHHHHHHEEECCEEEEEECHHHHCCCCCCCCHHCEEEEEEECCCCC
SLGAVAASDAFFPFSDGLDVLINSGVKAIVQPGGSVRDNAVIAAAQQAGVTMFFTGKRHF
CCCCEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCEEEEEHHHCCEEEEEECCHHC
AH
CC
>Mature Secondary Structure 
TTNIRIKRALISVSDKSGLADLAEALAAHSVKIVSTGSTAEFIRGVSIPVRDVSEVTGV
CCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHHHHCH
GELLDGRVKTLHPKIHAPILADTTSQMHRAQLQQLGVDAFDLVVVNLYPFFEISKNSEAE
HHHHCCCCEEECCCCCCCEEECCHHHHHHHHHHHCCCCHHHEEEEEEEEEEEECCCCCCH
FSDVIEQIDIGGSALIRAAAKNHTRVVVIVDPSDYIHVINSLERGAPSRLRHQLAIKAYS
HHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHEEEEC
HTSEYDLHISRWLSERFYKHTLYSSSDSVGDVCDNNLDSNDHFVELRGKKLGDLRYGENS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
HQKASLYLSCKESVPQLGLASAALLGGKQMSYNNYLDADVASRIVNSFDLPTVSFVKHGN
CCEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCCCC
PCGIASNIEVAIACRNAHECDPTSAFGGVVAVNREVTLDVATHLLPIFIEVVVAPGYDPQ
CCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEEHHHHHHHHHHHHHHEECCCCCHH
ALQLLLKKKNLRVVELPECASYPQDQLRQISGGFLVQDADKFDHHDTFSSWTQVSGPRIF
HHHHHHHHCCCEEEECCHHHCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCCCEEE
MDAGADNETLMDLEFAWKSCAFVKSNAILLAKNMASVGIGMGQVNRLDACYLAVNRAGSR
EECCCCCCEEEEHHHHHHHHHEEECCEEEEEECHHHHCCCCCCCCHHCEEEEEEECCCCC
SLGAVAASDAFFPFSDGLDVLINSGVKAIVQPGGSVRDNAVIAAAQQAGVTMFFTGKRHF
CCCCEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCEEEEEHHHCCEEEEEECCHHC
AH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA