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The map label for this gene is purN [H]

Identifier: 28572260

GI number: 28572260

Start: 92799

End: 93437

Strand: Direct

Name: purN [H]

Synonym: TW093

Alternate gene names: 28572260

Gene position: 92799-93437 (Clockwise)

Preceding gene: 28572259

Following gene: 28572261

Centisome position: 10.02

GC content: 44.76

Gene sequence:

>639_bases
ATGAAAACTGCTATGAGACTTATTGTTATGGTATCCGGGATTGGCTCAGGTCTCTTGCGTCTAATTCGAGCTTGCGAACA
AAAGGAGCTCAAAGCTGAGATTGTTGCCGTTGGCTCTGACAGGCATGCTCCAGCACTTTCGCATGCCTCAGATTATGGAA
TTCCATTTTTTGTATCCCCATTCAAAGAATATTCCAATCGTGACGCATGGGGGGCGAATCTACTAAACACCGTCTTGGCT
TACAAGCCTGATCTGGTCGTGTTGAGCGGATTTATGCGTATTCTGCCATCATGTGTTGTTGATGCTCTGTCACCTAATTT
GATCAACACACATCCATCGTATTTACCTGAGTTTCCCGGAATGAATGCCGTCGAGGATGCGTTGCGGGCCGGTGTAAAAA
CAACCGGTGCAAGTGTTATCAGGGTGGACAATGGAATTGACACGGGCCCTGTTATATCTCAGATGAGGGTTAAAGTCTAT
TCCTCTGACACATGTCAGACTTTACATAGTCGCATAAAAAAGGTTGAGCATCTGCTTTTATGTCGGGCTATCAAAAATAT
ATACACCGAGCAATTCATTCTAAAGAATCTTTTGCATAACAGCGTGAGGCGTCCCTCTGGTAAAACAGGAGATCGATGA

Upstream 100 bases:

>100_bases
AGATTGTGGGGCAAACAATCAAAAGTAGACCCAGCGATCAAAGGTAAAAGTCGTAATAAACCCGGGCTTGGCGTTCCAAG
AATGAAAGACCTTGAGTGAT

Downstream 100 bases:

>100_bases
ACGGGTCGGTAAATTTTAGTGATAAGTAATGTTTAGAATATGACGACAAATATACGTATAAAAAGAGCTCTTATTTCAGT
GAGTGATAAGTCAGGCCTTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKTAMRLIVMVSGIGSGLLRLIRACEQKELKAEIVAVGSDRHAPALSHASDYGIPFFVSPFKEYSNRDAWGANLLNTVLA
YKPDLVVLSGFMRILPSCVVDALSPNLINTHPSYLPEFPGMNAVEDALRAGVKTTGASVIRVDNGIDTGPVISQMRVKVY
SSDTCQTLHSRIKKVEHLLLCRAIKNIYTEQFILKNLLHNSVRRPSGKTGDR

Sequences:

>Translated_212_residues
MKTAMRLIVMVSGIGSGLLRLIRACEQKELKAEIVAVGSDRHAPALSHASDYGIPFFVSPFKEYSNRDAWGANLLNTVLA
YKPDLVVLSGFMRILPSCVVDALSPNLINTHPSYLPEFPGMNAVEDALRAGVKTTGASVIRVDNGIDTGPVISQMRVKVY
SSDTCQTLHSRIKKVEHLLLCRAIKNIYTEQFILKNLLHNSVRRPSGKTGDR
>Mature_212_residues
MKTAMRLIVMVSGIGSGLLRLIRACEQKELKAEIVAVGSDRHAPALSHASDYGIPFFVSPFKEYSNRDAWGANLLNTVLA
YKPDLVVLSGFMRILPSCVVDALSPNLINTHPSYLPEFPGMNAVEDALRAGVKTTGASVIRVDNGIDTGPVISQMRVKVY
SSDTCQTLHSRIKKVEHLLLCRAIKNIYTEQFILKNLLHNSVRRPSGKTGDR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=37.8378378378378, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=37.8378378378378, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=37.8378378378378, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1788846, Length=176, Percent_Identity=35.7954545454545, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787483, Length=193, Percent_Identity=30.0518134715026, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=33.1491712707182, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582400, Length=182, Percent_Identity=37.3626373626374, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23304; Mature: 23304

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAMRLIVMVSGIGSGLLRLIRACEQKELKAEIVAVGSDRHAPALSHASDYGIPFFVSP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCCEECH
FKEYSNRDAWGANLLNTVLAYKPDLVVLSGFMRILPSCVVDALSPNLINTHPSYLPEFPG
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MNAVEDALRAGVKTTGASVIRVDNGIDTGPVISQMRVKVYSSDTCQTLHSRIKKVEHLLL
CHHHHHHHHHCCHHCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CRAIKNIYTEQFILKNLLHNSVRRPSGKTGDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTAMRLIVMVSGIGSGLLRLIRACEQKELKAEIVAVGSDRHAPALSHASDYGIPFFVSP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCCEECH
FKEYSNRDAWGANLLNTVLAYKPDLVVLSGFMRILPSCVVDALSPNLINTHPSYLPEFPG
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MNAVEDALRAGVKTTGASVIRVDNGIDTGPVISQMRVKVYSSDTCQTLHSRIKKVEHLLL
CHHHHHHHHHCCHHCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CRAIKNIYTEQFILKNLLHNSVRRPSGKTGDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]