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The map label for this gene is punA [H]

Identifier: 28572193

GI number: 28572193

Start: 18061

End: 18894

Strand: Reverse

Name: punA [H]

Synonym: TW018

Alternate gene names: 28572193

Gene position: 18894-18061 (Counterclockwise)

Preceding gene: 28572195

Following gene: 28572192

Centisome position: 2.04

GC content: 50.12

Gene sequence:

>834_bases
ATGGAAGATATCTGCAATACTGAACTCACCGCTGCTACATTAGCTGCAAAACAGATTGCAGATATAACAGGTATTGCCCG
GCATGACATCGCCGTTACTTTGGGTAGCGGCTGGGTTGATGCTGTATCCAACCTCGGTGAGCTTATTACAACAATCCCCG
CGCAAGATATACACGGGTTTTTCCCTCCCGTTTTGGCTGGCCACAGCGGTGTTCTGACCTCCATTCGACTTACGTGCGGA
GTATATGTTCTTGTAATAGGCGCGAGGACTCATCTCTACCAAGGACATGGCCCTGAGGCTGTGGTGCATGGCATCAAAAC
CGCCCATGCAGCGGGGGCGAGGGTGGCAATCCTTACCAATGGCGCGGGAAGCACAGTGCCTGATTGGGGTCCTGGAGAGG
TGGTAGTAATAAAGGACCATATCAATCTGACCGGAAAATCTCCTCTAAGCGGGGCAAATTTTTGCGATATGAGCGATATT
TATACGGCCGCACTCAGGCAAAAAATCCTGCAAATGGCCCCCAATATCCCTCAAGGGGTGTACGCGCAAATGCCGGGTCC
TCAGTACGAAACACCGGCTGAGGTGAAAATGTTGAGAACTATCGGCGCAGATCTTGTCGGAATGTCGACAGCCCTTGAGG
CAATAATGGCAAAGTCACTAAAGATGAATGTTGTGGGGTTGTCTCTTGTGACAAACCTTGCGGCGGGAATAGGTACAGGA
CAATTGAGCCATGATGAAGTGCTGACTGTAAGCGAACAGGCTAGACAAAAAACTTCAGCATTTCTCGTAGAGCTCATTCG
TGACATCGGGCAAGATCTATCGGGCAGGAAATAA

Upstream 100 bases:

>100_bases
TGATTTTTCTTGACAGACACTCTCAGCCTTCTTACCAGACTCAAGTATAGTGCTGCTTGACGGCGGTGTTCTTGTTGGAT
TATCCTGGAAATCAGCCGCC

Downstream 100 bases:

>100_bases
CAGGGTAGCCATATGAATATTCTGCGGAAGGCAAGCTTGTGGATTGAGAATGATCCGGTGGCGATCACACGCTTGGAAAT
GCGCAGACTTCTGGCTGAAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEDICNTELTAATLAAKQIADITGIARHDIAVTLGSGWVDAVSNLGELITTIPAQDIHGFFPPVLAGHSGVLTSIRLTCG
VYVLVIGARTHLYQGHGPEAVVHGIKTAHAAGARVAILTNGAGSTVPDWGPGEVVVIKDHINLTGKSPLSGANFCDMSDI
YTAALRQKILQMAPNIPQGVYAQMPGPQYETPAEVKMLRTIGADLVGMSTALEAIMAKSLKMNVVGLSLVTNLAAGIGTG
QLSHDEVLTVSEQARQKTSAFLVELIRDIGQDLSGRK

Sequences:

>Translated_277_residues
MEDICNTELTAATLAAKQIADITGIARHDIAVTLGSGWVDAVSNLGELITTIPAQDIHGFFPPVLAGHSGVLTSIRLTCG
VYVLVIGARTHLYQGHGPEAVVHGIKTAHAAGARVAILTNGAGSTVPDWGPGEVVVIKDHINLTGKSPLSGANFCDMSDI
YTAALRQKILQMAPNIPQGVYAQMPGPQYETPAEVKMLRTIGADLVGMSTALEAIMAKSLKMNVVGLSLVTNLAAGIGTG
QLSHDEVLTVSEQARQKTSAFLVELIRDIGQDLSGRK
>Mature_277_residues
MEDICNTELTAATLAAKQIADITGIARHDIAVTLGSGWVDAVSNLGELITTIPAQDIHGFFPPVLAGHSGVLTSIRLTCG
VYVLVIGARTHLYQGHGPEAVVHGIKTAHAAGARVAILTNGAGSTVPDWGPGEVVVIKDHINLTGKSPLSGANFCDMSDI
YTAALRQKILQMAPNIPQGVYAQMPGPQYETPAEVKMLRTIGADLVGMSTALEAIMAKSLKMNVVGLSLVTNLAAGIGTG
QLSHDEVLTVSEQARQKTSAFLVELIRDIGQDLSGRK

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=267, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788746, Length=251, Percent_Identity=33.0677290836653, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17541190, Length=262, Percent_Identity=32.4427480916031, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323238, Length=199, Percent_Identity=35.678391959799, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=30.3249097472924, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=30.3249097472924, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656093, Length=277, Percent_Identity=30.3249097472924, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656090, Length=277, Percent_Identity=30.3249097472924, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24762376, Length=257, Percent_Identity=25.2918287937743, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 28992; Mature: 28992

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDICNTELTAATLAAKQIADITGIARHDIAVTLGSGWVDAVSNLGELITTIPAQDIHGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHC
FPPVLAGHSGVLTSIRLTCGVYVLVIGARTHLYQGHGPEAVVHGIKTAHAAGARVAILTN
CCHHHCCCCCHHHHHHHHHHHHHEEEECHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEC
GAGSTVPDWGPGEVVVIKDHINLTGKSPLSGANFCDMSDIYTAALRQKILQMAPNIPQGV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
YAQMPGPQYETPAEVKMLRTIGADLVGMSTALEAIMAKSLKMNVVGLSLVTNLAAGIGTG
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSHDEVLTVSEQARQKTSAFLVELIRDIGQDLSGRK
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEDICNTELTAATLAAKQIADITGIARHDIAVTLGSGWVDAVSNLGELITTIPAQDIHGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHC
FPPVLAGHSGVLTSIRLTCGVYVLVIGARTHLYQGHGPEAVVHGIKTAHAAGARVAILTN
CCHHHCCCCCHHHHHHHHHHHHHEEEECHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEC
GAGSTVPDWGPGEVVVIKDHINLTGKSPLSGANFCDMSDIYTAALRQKILQMAPNIPQGV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
YAQMPGPQYETPAEVKMLRTIGADLVGMSTALEAIMAKSLKMNVVGLSLVTNLAAGIGTG
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QLSHDEVLTVSEQARQKTSAFLVELIRDIGQDLSGRK
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10600382 [H]