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The map label for this gene is ahpF [H]

Identifier: 284159994

GI number: 284159994

Start: 722969

End: 724576

Strand: Reverse

Name: ahpF [H]

Synonym: BURPS668_A0756

Alternate gene names: 284159994

Gene position: 724576-722969 (Counterclockwise)

Preceding gene: 126442391

Following gene: 126445340

Centisome position: 23.17

GC content: 68.47

Gene sequence:

>1608_bases
ATGCTCGACGCCCATCTCAAGACTCAGTTGAAATCGTACCTCGAACGGATCACGCGCCCCGTCGAGCTCGTCGCATCGCT
CGACGCAGGCGACCAGTCGCGCGAATTGCGCGCGCTACTCGACGAGATCGCGGCGCTGTCGCCGCAGATCTTCGTGACCG
AGCGCCGCGACGCGGCTGAGCGCGCGCCGTCGTTCTCGGTCGGCGAGCCGGGCAAGGCCGCGGGTATTCGCTTCGCCGGT
ATGCCGATGGGCCACGAATTCACGTCGCTCGTGCTCGCGCTGCTGCAGACGGGCGGCCATCCGATCAAGCTCGACGACGC
GACGATCGAGCAGATCCGCGCGCTCGACGGCGACTACGCATTCGAGACGTATATCTCGCTCACCTGCCAGAACTGCCCGG
AAGTCGTGCAGGCGCTCAACGTGATGGCGCTCGTCAACCCGCGCATCCGCCACGTGATGATCGACGGCGCGCTGTTCCAG
GACGAAGTCGAGGCGCGCCGGATCATGGCCGTGCCGACGATCTTCCTGAACGGCGAGACGTTCGGCCAGGGCCGCAGCAG
CGTGAAGGAGATCCTCGCGAAACTCGACGGCGGCGCGAGCCGGCGCGCGGCGCAGGCGCTCGCGGAGAAACCCGTGTTCG
ACATGCTGATCGTCGGCGGCGGCCCCGCGGGCGCGGCGGCGGCGATCTACGCGGCGCGCAAGGGCATCTCGACGGGCGTC
GTCGCCGAGCGCTTCGGCGGCCAGGTGCTCGATACGATGGCGATCGAGAATTTCGTGTCGGTCCAGGAAACCGAGGGGCC
GAAGTTCGCGACCGCGCTCGAGCAGCACGTGAAGCAATACGATGTCGACGTGATCGACGTAGAGCGCGCCGACCGGCTCG
TGCCGGGCCGCGTTCATGAAATTCGCCTCGCGAGCGGCGCGGTGCTGAAGGCGAAGACCGTCGTGCTCGCGACGGGCGCG
CGCTGGCGCGAGATCGGCGTGCCCGGCGAGCGCGAGTATCGCAACCGCGGCGTCGCGTACTGCCCGCATTGCGACGGGCC
GCTCTTCAAGGGCAAGCGCGTCGCGGTGGTCGGCGGCGGCAACTCGGGTGTCGAGGCGGCGATCGATCTCGCCGGCCTTG
CCGCCGCCGTGACGCTGATCGAATACGGCGAGCAGTTGCGTGCGGACGACGTGCTGCAACGCAAACTGCGCAGCCTCGCG
AACGTGACGGTGATCACGCAGGCACGCACGACCGAGCTCACGGGCGACGGCGCGAAGCTGAACGGGCTCGTCTACGAGGA
CAGGCGCACAGGCGAGGCGAAGCGCATCGCGCTCGAAGGCGTGTTCGTGCAGATCGGCCTCGTGCCGAACACCGAATGGC
TGAAGGAAACCGTCGAGCTGTCGAGGCACGGCGAGATCGTCGTCGACGCGCGCGGCGCGACGTCGGTGCCCGGCGTGTTC
GCCGCCGGCGACGTGACGACGGTGCCGTACAAGCAGATCGTGATCGCGGTCGGCGAAGGCGCGAAGGCATCGCTCGGCGC
ATTCGACCACCTGATTCGAAGCGAGGCGCATGCCGTGCAGGCGGCGGATACCGAAGCTTCGGCAAACGCCGAGGCCATGG
CCGCCTGA

Upstream 100 bases:

>100_bases
CGCCCGTTTCGCGGCCCCGCTCCCGGCCTCACCCGCTCCATCGCCGCCTCGCCCGCGTTCGATGGGCAAGGCCGCCTTTC
GCTATGGAATCCGAATCGTC

Downstream 100 bases:

>100_bases
TTCGCGAAACGAGCCTTTCAACCGTCTACCGTTGGCCCTCGCCGCGATGTGATGAGCGTCGTGGCGGGGGCGCTTTTTGA
CGCATCGGCACTTTTTGCCC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLRADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDARGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADTEASANAEAMAA

Sequences:

>Translated_535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLRADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDARGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADTEASANAEAMAA
>Mature_535_residues
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAERAPSFSVGEPGKAAGIRFAG
MPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYAFETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQ
DEVEARRIMAVPTIFLNGETFGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHEIRLASGAVLKAKTVVLATGA
RWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGLAAAVTLIEYGEQLRADDVLQRKLRSLA
NVTVITQARTTELTGDGAKLNGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDARGATSVPGVF
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADTEASANAEAMAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=60.6936416184971, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=32.258064516129, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=32.3717948717949, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=30.7692307692308, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 57060; Mature: 57060

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAE
CCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
RAPSFSVGEPGKAAGIRFAGMPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYA
HCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCE
FETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQDEVEARRIMAVPTIFLNGET
EEHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCC
FGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCEE
IRLASGAVLKAKTVVLATGARWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGLAAAVTLIEYGEQLRADDVLQRKLRSLANVTVITQARTTELTGDGAKL
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCEEEECCCCCEE
NGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDARGATSVPGVF
CCEEEECCCCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADTEASANAEAMAA
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MLDAHLKTQLKSYLERITRPVELVASLDAGDQSRELRALLDEIAALSPQIFVTERRDAAE
CCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHH
RAPSFSVGEPGKAAGIRFAGMPMGHEFTSLVLALLQTGGHPIKLDDATIEQIRALDGDYA
HCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCE
FETYISLTCQNCPEVVQALNVMALVNPRIRHVMIDGALFQDEVEARRIMAVPTIFLNGET
EEHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCC
FGQGRSSVKEILAKLDGGASRRAAQALAEKPVFDMLIVGGGPAGAAAAIYAARKGISTGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDVIDVERADRLVPGRVHE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCEE
IRLASGAVLKAKTVVLATGARWREIGVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGLAAAVTLIEYGEQLRADDVLQRKLRSLANVTVITQARTTELTGDGAKL
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEEECCEEEECCCCCEE
NGLVYEDRRTGEAKRIALEGVFVQIGLVPNTEWLKETVELSRHGEIVVDARGATSVPGVF
CCEEEECCCCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLGAFDHLIRSEAHAVQAADTEASANAEAMAA
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]