Click here to switch to the map view.

The map label for this gene is purA

Identifier: 28379639

GI number: 28379639

Start: 2910392

End: 2911681

Strand: Direct

Name: purA

Synonym: lp_3270

Alternate gene names: 28379639

Gene position: 2910392-2911681 (Clockwise)

Preceding gene: 28379637

Following gene: 28379640

Centisome position: 87.97

GC content: 45.43

Gene sequence:

>1290_bases
ATGGCATCAGTCGTAGTAGTAGGTAGCCAGTGGGGCGATGAAGGTAAAGGAAAGATCACGGACTTTTTGAGTCAGGAAGC
AGATGTGGTATCACGTTACCAAGGCGGCGATAACGCCGGCCACACCATCGTATTTAATGGGCAAACTTTCAAATTACGGC
TGATTCCATCAGGGATTTTCTTCCATGATAAGTTAGCTGTCATTGGGAACGGCGTGGTCTTAAATCCCAAGTCATTAGTT
GAAGAATTACAATACTTGCGGGACAAGGGCGTTAATCCTGACAACCTTAGAATCTCTAACCGTGCACACGTCATCTTGCC
TTACCACATCACATTAGATGGCGCTCAAGAAAAAGCTAAAGCCGGTGGCAAGATTGGGACGACTAATAAGGGAATCGGCC
CAGCCTACATGGATAAGGCTGAACGAATTGGGATTCGCGTAGCCGACCTGCTTGATAAGGACACCTTTGCGGCGTTACTC
AAGCGCAACTTAGCTGAAAAGAACCAAATTATTACCAAGCTTTATGACTTAGAACCACTGAAATTTGAAGATATTTTTGA
TGACTATTACGCTTATGGTCAAACTTTGAAACCATTCGTCACCGATACTTCCGTTGTCATTAATGATGCGTTAGATAACG
GTCAACGCGTCCTATTTGAAGGTGCTCAGGGAGTCATGCTGGATATTGACCAAGGGACTTACCCTTACGTTACTTCATCT
AACCCAGTTGCCGGTGGTGTTACTATTGGGAGCGGTGTTGGCCCATCTAAAATCGATAACTGTGTTGGGGTCTTAAAAGC
CTACACTTCCCGCGTCGGTGACGGCCCATTCCCAACTGAACTCTTTGACGAAGTCGGTGATTTCATTCGTGAAACGGCCC
ATGAATATGGGACCGTTACTAAACGTCCTCGGCGGATTGGTTGGTTCGATAGCGTTGTATTACGGCATGCTAAACGAGTT
TCTGGGTTTACTCATCTTAGTTTGAACTGCTTAGACGTCTTGACGGGCTTAAAGACCATCAAGGTATGTACCGCTTATGA
CTTAAACGGCGAAACCATCTATCACTACCCTGCTAGTCTGAAGGAATTAGAAGCTTGCAAACCTATCTATGAAGAACTTC
CTGGCTGGGACGAAGACATTACTGGCGTTAAAACCTTTGAGGAACTGCCAACTAATGCACAGAACTACTTGCGTAAATTA
GAAGAACTCGTCGGCGTTAAAATTGCCACCTTCTCTGTTGGTCCCGATCGCCAACAAACTAACGTTATCGATCACAACAT
TTGGGGCTAA

Upstream 100 bases:

>100_bases
CGTTAAAGTTGCTATACTCTTTCCTGTTGGAGTGGTGAACGTTTAACTACTCTTTAGGATTAATTATTCATAATTAAACT
ATTTAATGAGGTGGAAGAAT

Downstream 100 bases:

>100_bases
TTGAACGTGACACCGTGAATTAACTTAAAGGAGTTTTGCATCATGGGCATGGAGATTTTTGATTACGAAGATATTCAACT
AATTCCGAACAAGTGTGTCA

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MASVVVVGSQWGDEGKGKITDFLSQEADVVSRYQGGDNAGHTIVFNGQTFKLRLIPSGIFFHDKLAVIGNGVVLNPKSLV
EELQYLRDKGVNPDNLRISNRAHVILPYHITLDGAQEKAKAGGKIGTTNKGIGPAYMDKAERIGIRVADLLDKDTFAALL
KRNLAEKNQIITKLYDLEPLKFEDIFDDYYAYGQTLKPFVTDTSVVINDALDNGQRVLFEGAQGVMLDIDQGTYPYVTSS
NPVAGGVTIGSGVGPSKIDNCVGVLKAYTSRVGDGPFPTELFDEVGDFIRETAHEYGTVTKRPRRIGWFDSVVLRHAKRV
SGFTHLSLNCLDVLTGLKTIKVCTAYDLNGETIYHYPASLKELEACKPIYEELPGWDEDITGVKTFEELPTNAQNYLRKL
EELVGVKIATFSVGPDRQQTNVIDHNIWG

Sequences:

>Translated_429_residues
MASVVVVGSQWGDEGKGKITDFLSQEADVVSRYQGGDNAGHTIVFNGQTFKLRLIPSGIFFHDKLAVIGNGVVLNPKSLV
EELQYLRDKGVNPDNLRISNRAHVILPYHITLDGAQEKAKAGGKIGTTNKGIGPAYMDKAERIGIRVADLLDKDTFAALL
KRNLAEKNQIITKLYDLEPLKFEDIFDDYYAYGQTLKPFVTDTSVVINDALDNGQRVLFEGAQGVMLDIDQGTYPYVTSS
NPVAGGVTIGSGVGPSKIDNCVGVLKAYTSRVGDGPFPTELFDEVGDFIRETAHEYGTVTKRPRRIGWFDSVVLRHAKRV
SGFTHLSLNCLDVLTGLKTIKVCTAYDLNGETIYHYPASLKELEACKPIYEELPGWDEDITGVKTFEELPTNAQNYLRKL
EELVGVKIATFSVGPDRQQTNVIDHNIWG
>Mature_428_residues
ASVVVVGSQWGDEGKGKITDFLSQEADVVSRYQGGDNAGHTIVFNGQTFKLRLIPSGIFFHDKLAVIGNGVVLNPKSLVE
ELQYLRDKGVNPDNLRISNRAHVILPYHITLDGAQEKAKAGGKIGTTNKGIGPAYMDKAERIGIRVADLLDKDTFAALLK
RNLAEKNQIITKLYDLEPLKFEDIFDDYYAYGQTLKPFVTDTSVVINDALDNGQRVLFEGAQGVMLDIDQGTYPYVTSSN
PVAGGVTIGSGVGPSKIDNCVGVLKAYTSRVGDGPFPTELFDEVGDFIRETAHEYGTVTKRPRRIGWFDSVVLRHAKRVS
GFTHLSLNCLDVLTGLKTIKVCTAYDLNGETIYHYPASLKELEACKPIYEELPGWDEDITGVKTFEELPTNAQNYLRKLE
ELVGVKIATFSVGPDRQQTNVIDHNIWG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=44.1805225653207, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI34577063, Length=423, Percent_Identity=43.2624113475177, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=42.6020408163265, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=48.4633569739953, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=42.688679245283, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=41.9811320754717, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=42.6229508196721, Blast_Score=335, Evalue=7e-93,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=45.1073985680191, Blast_Score=350, Evalue=8e-97,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_LACPL (Q88SV6)

Other databases:

- EMBL:   AL935261
- RefSeq:   NP_786531.1
- ProteinModelPortal:   Q88SV6
- SMR:   Q88SV6
- GeneID:   1062621
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_3270
- NMPDR:   fig|220668.1.peg.2665
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   CLSK2584391
- BioCyc:   LPLA220668:LP_3270-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47235; Mature: 47104

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVVVVGSQWGDEGKGKITDFLSQEADVVSRYQGGDNAGHTIVFNGQTFKLRLIPSGIF
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCE
FHDKLAVIGNGVVLNPKSLVEELQYLRDKGVNPDNLRISNRAHVILPYHITLDGAQEKAK
EECCCEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCHHHHH
AGGKIGTTNKGIGPAYMDKAERIGIRVADLLDKDTFAALLKRNLAEKNQIITKLYDLEPL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KFEDIFDDYYAYGQTLKPFVTDTSVVINDALDNGQRVLFEGAQGVMLDIDQGTYPYVTSS
CHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIDNCVGVLKAYTSRVGDGPFPTELFDEVGDFIRETAHEYGTVT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KRPRRIGWFDSVVLRHAKRVSGFTHLSLNCLDVLTGLKTIKVCTAYDLNGETIYHYPASL
CCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHCHHHEEEEEEEECCCCEEEECCCCH
KELEACKPIYEELPGWDEDITGVKTFEELPTNAQNYLRKLEELVGVKIATFSVGPDRQQT
HHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
NVIDHNIWG
CCCCCCCCC
>Mature Secondary Structure 
ASVVVVGSQWGDEGKGKITDFLSQEADVVSRYQGGDNAGHTIVFNGQTFKLRLIPSGIF
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCE
FHDKLAVIGNGVVLNPKSLVEELQYLRDKGVNPDNLRISNRAHVILPYHITLDGAQEKAK
EECCCEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCHHHHH
AGGKIGTTNKGIGPAYMDKAERIGIRVADLLDKDTFAALLKRNLAEKNQIITKLYDLEPL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KFEDIFDDYYAYGQTLKPFVTDTSVVINDALDNGQRVLFEGAQGVMLDIDQGTYPYVTSS
CHHHHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKIDNCVGVLKAYTSRVGDGPFPTELFDEVGDFIRETAHEYGTVT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KRPRRIGWFDSVVLRHAKRVSGFTHLSLNCLDVLTGLKTIKVCTAYDLNGETIYHYPASL
CCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHCHHHEEEEEEEECCCCEEEECCCCH
KELEACKPIYEELPGWDEDITGVKTFEELPTNAQNYLRKLEELVGVKIATFSVGPDRQQT
HHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
NVIDHNIWG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566