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The map label for this gene is thiN [H]

Identifier: 28378322

GI number: 28378322

Start: 1476987

End: 1477646

Strand: Direct

Name: thiN [H]

Synonym: lp_1622

Alternate gene names: 28378322

Gene position: 1476987-1477646 (Clockwise)

Preceding gene: 28378321

Following gene: 28378324

Centisome position: 44.65

GC content: 48.94

Gene sequence:

>660_bases
ATGGCAACCATCGTAAACTTACTGGTTGGCGGACCGACCGCTAATTATCCAGCAGATTTAACGACGATTCCAGGACCATG
GGTCGGCGCTGACCGTGGCGCGTTGCGTTTGGTGAAACGTGGTATCCAGCCCGTGATGGTCGTAGGTGATTTTGATTCAA
TTGATGCGGCTGAACTCCAGACTGTCAAAGATGCGTTGGTGGGCGCTATCGTTGTCAAACCCGACCAAGACCATACGGAT
ACACAATTGGCGATTAAATCCATTTTCGAGCAGTTGCAACCGGATGAAGTGCATCTATATGGTGCCACGGGTGGTCGGTT
AGACCATTTACTGGCCAATATGTGGTTAGTACTTGATCCGGTATTTCGTCAGTGGGCTCCGCAGATCAAACTGATTGATA
AGCAAAATTCGGTTCGGTTCTTTCTACCAGGGGACTACCAGATCACGAAGGAAGCGGATAAACGCTATTTGGCGTTTGTA
CCGCTGATGCCGATGCATCTCACGTTACCCGATGAAAAGTATCAGTTGGACGCTGCTTATAATGCGTACCCGATTTCATG
GGCCAGTAACGAATTCAGTGGCAATACTGGTCACTTTAGCTTTGATGCGGGGGTTCTAGCTGTTATTCAAAGTCGTGATG
ACAGCATGGCAGATGCGTAG

Upstream 100 bases:

>100_bases
GCTATGACGCTGGTGCTACGGTCGCTGTTGCTGGTTCGTATGTCTATGACAGTGCGGACCCTGCACAACGTATTCAAGAT
TTGAAGGTGGCGACCAATTA

Downstream 100 bases:

>100_bases
GGTACCCCAAGGTTTCAGCTAAAATAAGACCGGTCGGTGAGTCATAAACTCATCAACCGGTCTTTTAATATGCAGTAACG
ACGTATCATCGGGGAGCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MATIVNLLVGGPTANYPADLTTIPGPWVGADRGALRLVKRGIQPVMVVGDFDSIDAAELQTVKDALVGAIVVKPDQDHTD
TQLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDPVFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFV
PLMPMHLTLPDEKYQLDAAYNAYPISWASNEFSGNTGHFSFDAGVLAVIQSRDDSMADA

Sequences:

>Translated_219_residues
MATIVNLLVGGPTANYPADLTTIPGPWVGADRGALRLVKRGIQPVMVVGDFDSIDAAELQTVKDALVGAIVVKPDQDHTD
TQLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDPVFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFV
PLMPMHLTLPDEKYQLDAAYNAYPISWASNEFSGNTGHFSFDAGVLAVIQSRDDSMADA
>Mature_218_residues
ATIVNLLVGGPTANYPADLTTIPGPWVGADRGALRLVKRGIQPVMVVGDFDSIDAAELQTVKDALVGAIVVKPDQDHTDT
QLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDPVFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFVP
LMPMHLTLPDEKYQLDAAYNAYPISWASNEFSGNTGHFSFDAGVLAVIQSRDDSMADA

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24125; Mature: 23994

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIVNLLVGGPTANYPADLTTIPGPWVGADRGALRLVKRGIQPVMVVGDFDSIDAAELQ
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHH
TVKDALVGAIVVKPDQDHTDTQLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDP
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFVPLMPMHLTLPDEKYQLDAAY
HHHHCCCCEEEEECCCCEEEEECCCEEEECCCCCEEEEEECCCCEEEECCCCCEEEEECC
NAYPISWASNEFSGNTGHFSFDAGVLAVIQSRDDSMADA
CEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ATIVNLLVGGPTANYPADLTTIPGPWVGADRGALRLVKRGIQPVMVVGDFDSIDAAELQ
CEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHH
TVKDALVGAIVVKPDQDHTDTQLAIKSIFEQLQPDEVHLYGATGGRLDHLLANMWLVLDP
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VFRQWAPQIKLIDKQNSVRFFLPGDYQITKEADKRYLAFVPLMPMHLTLPDEKYQLDAAY
HHHHCCCCEEEEECCCCEEEEECCCEEEECCCCCEEEEEECCCCEEEECCCCCEEEEECC
NAYPISWASNEFSGNTGHFSFDAGVLAVIQSRDDSMADA
CEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]