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The map label for this gene is folD

Identifier: 28378300

GI number: 28378300

Start: 1454569

End: 1455438

Strand: Direct

Name: folD

Synonym: lp_1599

Alternate gene names: 28378300

Gene position: 1454569-1455438 (Clockwise)

Preceding gene: 28378299

Following gene: 28378301

Centisome position: 43.97

GC content: 48.85

Gene sequence:

>870_bases
GTGGCACTTGTGACGAAGATTATTGATGGTAAGGCAGTAGCTAAGAAAGTTAACGCGCAGACGGCAACGGCAGTAGCGGA
ATTGGCTGCTCAGGGAATTCAACCAGGGATTGCTGTAATTATTGTTGGGGACGATGCAGCCAGTCAAATCTATGTTCGAA
ATAAGAATCGTAAAGCCACTAAGTTGGGGATGCACTCCGTGGTGCGGCAGTTGCCAGCGACTACTAGTCAGGACGAACTG
TTAGCCATTATTGCGGCATACAATGCGGATGACAGCATTCATGGCATCTTGGTGCAGTCGCCACTACCAGCCCAAATTAA
TGAACCATTGATCACGATGGCCATTGATCCTAAAAAGGACGTCGATGGTTTTCACCCGACGAACGTTGGTAAGTTGATCA
CCAATTTTCCAGGTAATTATCCTGTAGCAAATACGCCTCGCGGTATTATGACGATGTTGGCTGACTATGGGGTTGATCCG
GCTGGTAAAACGGCGGTTGTTATTGGTCGGAGTACCATTGTTGGTAAGCCGATGGCCGCTTTGTTAACAAATGCTAACGC
AACTGTAACGATTGCACATAGTAAAACAGCTGACCTAAAGGCGGTCGCCCGGACGGCGGATATCTTGGTGGTTGCAACGG
GGATTGCACACCTGATCACTGGTGCTGATATCAAACCAGGCGCGACGGTCATTGATGTTGGCATGGATCGTGATGAAAAT
GGTAAACTAGTGGGTGACGTCGATTTTGATTCTGCTCAAGGCATCGCAGGGTTGATTACGCCCGTTCCTGGTGGTGTGGG
TCCAATGACGATTGCCACCTTGATGCAGACAACGGTGGAACTTGCAAAGTGGAGTGATGTAAGTGAGTGA

Upstream 100 bases:

>100_bases
CTGGCGATTTGCCAGACTTTTTTTAATAACTTATTAGTAAACCGGTCATAAATCATTGTGGTGAATATGCTAAAATAAGA
CCAGACAGAGAAAGAGGAGT

Downstream 100 bases:

>100_bases
AAGTCAACAATATTTGACAGTGACAGCTTTAACTCAGTATTTAAAACGTAAATTCGAGGTTGATCCGTATTTAGGTAAAG
TGTACCTAACGGGGGAAGTT

Product: bifunctional protein: methylenetetrahydrofolate dehydrogenase; methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MALVTKIIDGKAVAKKVNAQTATAVAELAAQGIQPGIAVIIVGDDAASQIYVRNKNRKATKLGMHSVVRQLPATTSQDEL
LAIIAAYNADDSIHGILVQSPLPAQINEPLITMAIDPKKDVDGFHPTNVGKLITNFPGNYPVANTPRGIMTMLADYGVDP
AGKTAVVIGRSTIVGKPMAALLTNANATVTIAHSKTADLKAVARTADILVVATGIAHLITGADIKPGATVIDVGMDRDEN
GKLVGDVDFDSAQGIAGLITPVPGGVGPMTIATLMQTTVELAKWSDVSE

Sequences:

>Translated_289_residues
MALVTKIIDGKAVAKKVNAQTATAVAELAAQGIQPGIAVIIVGDDAASQIYVRNKNRKATKLGMHSVVRQLPATTSQDEL
LAIIAAYNADDSIHGILVQSPLPAQINEPLITMAIDPKKDVDGFHPTNVGKLITNFPGNYPVANTPRGIMTMLADYGVDP
AGKTAVVIGRSTIVGKPMAALLTNANATVTIAHSKTADLKAVARTADILVVATGIAHLITGADIKPGATVIDVGMDRDEN
GKLVGDVDFDSAQGIAGLITPVPGGVGPMTIATLMQTTVELAKWSDVSE
>Mature_288_residues
ALVTKIIDGKAVAKKVNAQTATAVAELAAQGIQPGIAVIIVGDDAASQIYVRNKNRKATKLGMHSVVRQLPATTSQDELL
AIIAAYNADDSIHGILVQSPLPAQINEPLITMAIDPKKDVDGFHPTNVGKLITNFPGNYPVANTPRGIMTMLADYGVDPA
GKTAVVIGRSTIVGKPMAALLTNANATVTIAHSKTADLKAVARTADILVVATGIAHLITGADIKPGATVIDVGMDRDENG
KLVGDVDFDSAQGIAGLITPVPGGVGPMTIATLMQTTVELAKWSDVSE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=44.0972222222222, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=43.6860068259386, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.320819112628, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=26.457399103139, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=49.8207885304659, Blast_Score=252, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=39.1752577319588, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=42.4137931034483, Blast_Score=216, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=40.4761904761905, Blast_Score=198, Evalue=9e-52,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.5986394557823, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.5986394557823, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=45.4861111111111, Blast_Score=224, Evalue=6e-59,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=45.4861111111111, Blast_Score=224, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=45.4861111111111, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=45.4861111111111, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_LACPL (Q88WM8)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785192.1
- HSSP:   P11586
- ProteinModelPortal:   Q88WM8
- SMR:   Q88WM8
- GeneID:   1063585
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1599
- NMPDR:   fig|220668.1.peg.1326
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- ProtClustDB:   CLSK821313
- BioCyc:   LPLA220668:LP_1599-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29867; Mature: 29736

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVTKIIDGKAVAKKVNAQTATAVAELAAQGIQPGIAVIIVGDDAASQIYVRNKNRKAT
CCCEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCHH
KLGMHSVVRQLPATTSQDELLAIIAAYNADDSIHGILVQSPLPAQINEPLITMAIDPKKD
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEECCCCC
VDGFHPTNVGKLITNFPGNYPVANTPRGIMTMLADYGVDPAGKTAVVIGRSTIVGKPMAA
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
LLTNANATVTIAHSKTADLKAVARTADILVVATGIAHLITGADIKPGATVIDVGMDRDEN
HHCCCCEEEEEEECCCCHHHHHHHHHCEEEEHHHHHHHHCCCCCCCCCEEEEECCCCCCC
GKLVGDVDFDSAQGIAGLITPVPGGVGPMTIATLMQTTVELAKWSDVSE
CCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALVTKIIDGKAVAKKVNAQTATAVAELAAQGIQPGIAVIIVGDDAASQIYVRNKNRKAT
CCEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCHH
KLGMHSVVRQLPATTSQDELLAIIAAYNADDSIHGILVQSPLPAQINEPLITMAIDPKKD
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEECCCCC
VDGFHPTNVGKLITNFPGNYPVANTPRGIMTMLADYGVDPAGKTAVVIGRSTIVGKPMAA
CCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHH
LLTNANATVTIAHSKTADLKAVARTADILVVATGIAHLITGADIKPGATVIDVGMDRDEN
HHCCCCEEEEEEECCCCHHHHHHHHHCEEEEHHHHHHHHCCCCCCCCCEEEEECCCCCCC
GKLVGDVDFDSAQGIAGLITPVPGGVGPMTIATLMQTTVELAKWSDVSE
CCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566