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The map label for this gene is glnA [H]

Identifier: 28378285

GI number: 28378285

Start: 1443550

End: 1444896

Strand: Direct

Name: glnA [H]

Synonym: lp_1581

Alternate gene names: 28378285

Gene position: 1443550-1444896 (Clockwise)

Preceding gene: 28378284

Following gene: 28378286

Centisome position: 43.63

GC content: 43.95

Gene sequence:

>1347_bases
ATGGCAAAACAGTCCTACTCAAAAGACGATATCCGCCGCATCGTCAAAGAAGAAAACGTTAATTTTTTACGGTTAATGTT
TACTGATCTATTCGGCACAATTAAGAACGTTGAAGTTCCAGTTTCACAACTCGACAAACTCTTGGATAATAAGTTGATGT
TTGACGGTTCATCCATCGATGGGTTTGTTCGGATCGAAGAAAGTGACATGTACCTGTATCCTGACTTATCAACTTGGTTG
ATCATGCCTTGGAACACGGAACATGGTAAAATTGCACGGATTATCTGTGAAGTTTACACGTCTGACCGTAAGCCGTTCGA
AGGGGATCCACGGAACAACTTAATTCGTGTCTTGAACGATATGCGCGAAGCAGGTTATACGTCATTTAACTGTGGGACTG
AGCCTGAATTCTTCTTATTCAAGATGAATGAAAAGGGTGAACCAACGACCGAATTGAATGATAAGGGAAGTTACTTCGAC
TTATCACCAATGGACTTGGGTGAAAATTGCCGCCGGGACATTGCACTTGAACTTGAACGACTTGGTTTTAACGTTGAAGC
CAGCCATCATGAAGTTGCACCTGGTCAACATGAAATTGACTTTAAGTACGCGGATGCTTTATCTGCAGCCGATCATATCC
AAACGTTCAAATTGGTCGTTAAGACCATCGCACGGAAATACAATTTGTGGGCAACCTTTATGCCTAAGCCGTTAAACGGT
GTCAATGGTTCTGGGATGCATGTGAACATGTCCTTGTTCCATGATCAAGGCAATGCCTTTTATGATGCTAATGATAAGAA
TGGCTTAGAATTATCATCAGACGCTTATCACTTCTTAGGTGGTTTGATGAAGCATGCCCGGTCATACACTGCTGTCACTA
ACCCGACAGTCAACTCATACAAACGCTTGGTTCCTGGATATGAAGCGCCCGTTTACGTGGCCTGGTCAGCATCTAACCGG
TCACCAATGATTCGGATTCCAAGTGCACGGGGCTTGTCAACGCGCCTTGAATTACGGAGTGTGGACGCTTCAACTAACCC
ATATTTGGCCTTTGCAGCGGTTCTTGAAGCTGGCTTGGACGGAATCAAGAACGGTATTGAACCACCAAAGAGTGTTGATC
GTAATATCTATGTTATGGATGAAGATGAACGGGCTGCAGCTGGTATCGCTGACTTACCATCAACGTTACACAATGCGCTG
AAAGAGTTCCAGACAGATCCGACGATGAAGAAGGCGTTAGGGCCACATATTTATCAGAGTTTCTTGGAAGCTAAACGGCT
TGAATGGGCTTCATACCGTCAACAAGTTAGTGAATGGGAACGTGATCAGTACATGGAATTGTACTAA

Upstream 100 bases:

>100_bases
TTCCTGAATCTCGGAGGCTTGCAGTCCGACAAGGGCCCCAATTATCCGGCACACTAAGATGTTTGCATTCGTTAGTCAAT
CAATTTAAGGAGCGATTTTC

Downstream 100 bases:

>100_bases
ACCATTAATTGAGATAAAAATACGACGGTTAGGGCACCTTAAATGGCGAACTAGCCGTTTTTGTTATAAAATTGGTAAAT
TAGTGGTGTTAACCTTTATT

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAKQSYSKDDIRRIVKEENVNFLRLMFTDLFGTIKNVEVPVSQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWL
IMPWNTEHGKIARIICEVYTSDRKPFEGDPRNNLIRVLNDMREAGYTSFNCGTEPEFFLFKMNEKGEPTTELNDKGSYFD
LSPMDLGENCRRDIALELERLGFNVEASHHEVAPGQHEIDFKYADALSAADHIQTFKLVVKTIARKYNLWATFMPKPLNG
VNGSGMHVNMSLFHDQGNAFYDANDKNGLELSSDAYHFLGGLMKHARSYTAVTNPTVNSYKRLVPGYEAPVYVAWSASNR
SPMIRIPSARGLSTRLELRSVDASTNPYLAFAAVLEAGLDGIKNGIEPPKSVDRNIYVMDEDERAAAGIADLPSTLHNAL
KEFQTDPTMKKALGPHIYQSFLEAKRLEWASYRQQVSEWERDQYMELY

Sequences:

>Translated_448_residues
MAKQSYSKDDIRRIVKEENVNFLRLMFTDLFGTIKNVEVPVSQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWL
IMPWNTEHGKIARIICEVYTSDRKPFEGDPRNNLIRVLNDMREAGYTSFNCGTEPEFFLFKMNEKGEPTTELNDKGSYFD
LSPMDLGENCRRDIALELERLGFNVEASHHEVAPGQHEIDFKYADALSAADHIQTFKLVVKTIARKYNLWATFMPKPLNG
VNGSGMHVNMSLFHDQGNAFYDANDKNGLELSSDAYHFLGGLMKHARSYTAVTNPTVNSYKRLVPGYEAPVYVAWSASNR
SPMIRIPSARGLSTRLELRSVDASTNPYLAFAAVLEAGLDGIKNGIEPPKSVDRNIYVMDEDERAAAGIADLPSTLHNAL
KEFQTDPTMKKALGPHIYQSFLEAKRLEWASYRQQVSEWERDQYMELY
>Mature_447_residues
AKQSYSKDDIRRIVKEENVNFLRLMFTDLFGTIKNVEVPVSQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWLI
MPWNTEHGKIARIICEVYTSDRKPFEGDPRNNLIRVLNDMREAGYTSFNCGTEPEFFLFKMNEKGEPTTELNDKGSYFDL
SPMDLGENCRRDIALELERLGFNVEASHHEVAPGQHEIDFKYADALSAADHIQTFKLVVKTIARKYNLWATFMPKPLNGV
NGSGMHVNMSLFHDQGNAFYDANDKNGLELSSDAYHFLGGLMKHARSYTAVTNPTVNSYKRLVPGYEAPVYVAWSASNRS
PMIRIPSARGLSTRLELRSVDASTNPYLAFAAVLEAGLDGIKNGIEPPKSVDRNIYVMDEDERAAAGIADLPSTLHNALK
EFQTDPTMKKALGPHIYQSFLEAKRLEWASYRQQVSEWERDQYMELY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=461, Percent_Identity=25.1626898047722, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1790301, Length=439, Percent_Identity=39.6355353075171, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI87081870, Length=460, Percent_Identity=26.7391304347826, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50973; Mature: 50842

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQSYSKDDIRRIVKEENVNFLRLMFTDLFGTIKNVEVPVSQLDKLLDNKLMFDGSSID
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLSTWLIMPWNTEHGKIARIICEVYTSDRKPFEGDPRNNLIRVLND
EEEEEECCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MREAGYTSFNCGTEPEFFLFKMNEKGEPTTELNDKGSYFDLSPMDLGENCRRDIALELER
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
LGFNVEASHHEVAPGQHEIDFKYADALSAADHIQTFKLVVKTIARKYNLWATFMPKPLNG
CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
VNGSGMHVNMSLFHDQGNAFYDANDKNGLELSSDAYHFLGGLMKHARSYTAVTNPTVNSY
CCCCCEEEEEEEEECCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHCCCEECCCCHHHH
KRLVPGYEAPVYVAWSASNRSPMIRIPSARGLSTRLELRSVDASTNPYLAFAAVLEAGLD
HHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
GIKNGIEPPKSVDRNIYVMDEDERAAAGIADLPSTLHNALKEFQTDPTMKKALGPHIYQS
HHHHCCCCCHHHCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHH
FLEAKRLEWASYRQQVSEWERDQYMELY
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQSYSKDDIRRIVKEENVNFLRLMFTDLFGTIKNVEVPVSQLDKLLDNKLMFDGSSID
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLSTWLIMPWNTEHGKIARIICEVYTSDRKPFEGDPRNNLIRVLND
EEEEEECCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MREAGYTSFNCGTEPEFFLFKMNEKGEPTTELNDKGSYFDLSPMDLGENCRRDIALELER
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
LGFNVEASHHEVAPGQHEIDFKYADALSAADHIQTFKLVVKTIARKYNLWATFMPKPLNG
CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
VNGSGMHVNMSLFHDQGNAFYDANDKNGLELSSDAYHFLGGLMKHARSYTAVTNPTVNSY
CCCCCEEEEEEEEECCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHCCCEECCCCHHHH
KRLVPGYEAPVYVAWSASNRSPMIRIPSARGLSTRLELRSVDASTNPYLAFAAVLEAGLD
HHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
GIKNGIEPPKSVDRNIYVMDEDERAAAGIADLPSTLHNALKEFQTDPTMKKALGPHIYQS
HHHHCCCCCHHHCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHH
FLEAKRLEWASYRQQVSEWERDQYMELY
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA