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The map label for this gene is trmB

Identifier: 28378177

GI number: 28378177

Start: 1334437

End: 1335081

Strand: Direct

Name: trmB

Synonym: lp_1458

Alternate gene names: 28378177

Gene position: 1334437-1335081 (Clockwise)

Preceding gene: 28378176

Following gene: 28378179

Centisome position: 40.34

GC content: 46.51

Gene sequence:

>645_bases
ATGCGTGTCAGAAATAAACCGTGGGCGCCAAAGTTAATTGCCGCCCATCCCGAACTTATTACCGAAGATCCAACTCAGCT
AAAAGGCCGCTGGCAAAGCCGTTTTGCCAAACCCCAGCCACTCCAGATTGAAGTGGGGAGTGGAAAGGGCCAGTTCATCA
TTGAAATGGCTAAGCGTCATCCTGAGATAAATTACGTTGCCATTGAAATTCAGACCTCGGTGATTGCGATTATTTTGAAA
AAGCTAGTCGAGGCCCCGTTGCCTAACCTGCAATTGGCACATGCGGACGGTCAAGCGGTGACTGCCTTTTTCGAACCCCA
TGAAGTTGATCGCTTATATCTGAACTTCTCTGATCCGTGGCCGAAGTCGCGTCATGAAAAGCGGCGTTTGACCTACAAGT
CGTTTTTGAGCAGTTACCGGGAAGTCTTAAAACCCAACGGTCAGATCGAATTTAAGACTGATAACCGGGGGCTATTCGAA
TTTTCATTAACGAGCATGAATAACTTTGGGATGCAATTTGAACAAGTTTGGTTAGACTTGCATGCAGTTGCCACACCTGA
GGATAACGTCGAGACTGAATATGAACAGAAGTTCAGTGCGGCGGGCCCAATTTATAAGATTATTGCAACTTTCCCAGCAA
AATAG

Upstream 100 bases:

>100_bases
CAGTACGAAGTGTAACGACGAGCAACAAAATCGCCGCGAGTCCCAGATGGAATTCGCGGCGATTTTGTTGAGTAAGCAAA
TGAAGAAGGAGTCAATCAAG

Downstream 100 bases:

>100_bases
ACTGAATGAGAACAGCGAAACGCGTCGGCGATCGCTGTTTTTTAATTGAGTGTAATTGTCGGAAACATCAAAAATGAGCT
TCCAGAATTTTAATTCCAGA

Product: methyl transferase (putative)

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRVRNKPWAPKLIAAHPELITEDPTQLKGRWQSRFAKPQPLQIEVGSGKGQFIIEMAKRHPEINYVAIEIQTSVIAIILK
KLVEAPLPNLQLAHADGQAVTAFFEPHEVDRLYLNFSDPWPKSRHEKRRLTYKSFLSSYREVLKPNGQIEFKTDNRGLFE
FSLTSMNNFGMQFEQVWLDLHAVATPEDNVETEYEQKFSAAGPIYKIIATFPAK

Sequences:

>Translated_214_residues
MRVRNKPWAPKLIAAHPELITEDPTQLKGRWQSRFAKPQPLQIEVGSGKGQFIIEMAKRHPEINYVAIEIQTSVIAIILK
KLVEAPLPNLQLAHADGQAVTAFFEPHEVDRLYLNFSDPWPKSRHEKRRLTYKSFLSSYREVLKPNGQIEFKTDNRGLFE
FSLTSMNNFGMQFEQVWLDLHAVATPEDNVETEYEQKFSAAGPIYKIIATFPAK
>Mature_214_residues
MRVRNKPWAPKLIAAHPELITEDPTQLKGRWQSRFAKPQPLQIEVGSGKGQFIIEMAKRHPEINYVAIEIQTSVIAIILK
KLVEAPLPNLQLAHADGQAVTAFFEPHEVDRLYLNFSDPWPKSRHEKRRLTYKSFLSSYREVLKPNGQIEFKTDNRGLFE
FSLTSMNNFGMQFEQVWLDLHAVATPEDNVETEYEQKFSAAGPIYKIIATFPAK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=121, Percent_Identity=31.404958677686, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_LACPL (Q88WZ9)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785069.1
- ProteinModelPortal:   Q88WZ9
- SMR:   Q88WZ9
- GeneID:   1062373
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1458
- NMPDR:   fig|220668.1.peg.1203
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   CLSK2520208
- BioCyc:   LPLA220668:LP_1458-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24607; Mature: 24607

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRNKPWAPKLIAAHPELITEDPTQLKGRWQSRFAKPQPLQIEVGSGKGQFIIEMAKRH
CCCCCCCCCCCEEECCCCHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEHHHC
PEINYVAIEIQTSVIAIILKKLVEAPLPNLQLAHADGQAVTAFFEPHEVDRLYLNFSDPW
CCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCC
PKSRHEKRRLTYKSFLSSYREVLKPNGQIEFKTDNRGLFEFSLTSMNNFGMQFEQVWLDL
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
HAVATPEDNVETEYEQKFSAAGPIYKIIATFPAK
HEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCC
>Mature Secondary Structure
MRVRNKPWAPKLIAAHPELITEDPTQLKGRWQSRFAKPQPLQIEVGSGKGQFIIEMAKRH
CCCCCCCCCCCEEECCCCHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEHHHC
PEINYVAIEIQTSVIAIILKKLVEAPLPNLQLAHADGQAVTAFFEPHEVDRLYLNFSDPW
CCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCC
PKSRHEKRRLTYKSFLSSYREVLKPNGQIEFKTDNRGLFEFSLTSMNNFGMQFEQVWLDL
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
HAVATPEDNVETEYEQKFSAAGPIYKIIATFPAK
HEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566