Click here to switch to the map view.

The map label for this gene is rfbD [H]

Identifier: 28377964

GI number: 28377964

Start: 1087414

End: 1088256

Strand: Direct

Name: rfbD [H]

Synonym: lp_1190

Alternate gene names: 28377964

Gene position: 1087414-1088256 (Clockwise)

Preceding gene: 28377963

Following gene: 28377971

Centisome position: 32.87

GC content: 42.82

Gene sequence:

>843_bases
ATGGCTAAAGTAATGATTGTTGGTGCCAATGGTCAGTTAGGTCATGAGTTACAACGACTATTAAATGAACAAGAGATTGC
ATTTGACGCGCTGACTGAAAAAGAATTGGATATTACAGACTTAGACGCAGTCAAAGCTAAGATTGCCGAATTACAACCAG
CGGTCTTATATGATTGTGCGGCTTACACAGCAGTTGATAAGGCGGAAGATGAAGGCAAAGCCCTCAATTGGCTGGTCAAT
GTTGATGGAACAAAAAATTTAGCCCAAGTAGCAGCTGAACAGGGTGTTAAATTGGTTTATGTTTCTACTGACTACATTTT
TGATGGCACTAATAAAGGTGAATATTTAGAAGATGATCCAGCCAACCCCAAGAATGAGTATGGGCGTGCTAAGTGGGCTG
GTGAAGAAGCCGTTCGTCACAGCGGTGCTGATTACTATATTGTTCGGACTAGCTGGGTTTTCGGTGAATTTGGGCATAAC
TTTGTCTTCACAATGCAGAATTTAGCAAAGACGCATGACAAGTTGACAGTCGTCAATGATCAACTCGGACGGCCAACCTG
GACGCGAACATTAGCAGAATTTATGGCCCACCTGGTGAAAACTGAAGCCGCTTCAGGGACCTATCAATTAAGTAACGATA
ATACAGCTACTTGGTATGACTTTGCGAAGGAAATTTTGAAGAATACTGATGTGGAAGTAGCACCAGTTACTTCTGAACAA
TTCCCACAAAAGGCTTACCGGCCACAGCATTCAGTGATGAATTTGGATAAGGCTAAAGCAACTGGTTACCATATTTTGAC
CTGGCAGGAAGCACTGAGTGAGTTTCTAGAGAGCCTTGGGTAG

Upstream 100 bases:

>100_bases
TGAATCAAACTACGCCAAGAACGGGCAATAAAAACACAGAAGATTTTGAATAAGCGGAGTGTCGATTGACAGTTCGCTTA
TTTTTTAGGAGGAAATGAAA

Downstream 100 bases:

>100_bases
CAGAGAGAATATAATAAAAAGCCACTAAGTATTGTGTACTAGGCCCTGTCAAGTTGACAGATGAAATAATATAAAGCTTT
GTTTGCTTTTAAATGGCTTC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAKVMIVGANGQLGHELQRLLNEQEIAFDALTEKELDITDLDAVKAKIAELQPAVLYDCAAYTAVDKAEDEGKALNWLVN
VDGTKNLAQVAAEQGVKLVYVSTDYIFDGTNKGEYLEDDPANPKNEYGRAKWAGEEAVRHSGADYYIVRTSWVFGEFGHN
FVFTMQNLAKTHDKLTVVNDQLGRPTWTRTLAEFMAHLVKTEAASGTYQLSNDNTATWYDFAKEILKNTDVEVAPVTSEQ
FPQKAYRPQHSVMNLDKAKATGYHILTWQEALSEFLESLG

Sequences:

>Translated_280_residues
MAKVMIVGANGQLGHELQRLLNEQEIAFDALTEKELDITDLDAVKAKIAELQPAVLYDCAAYTAVDKAEDEGKALNWLVN
VDGTKNLAQVAAEQGVKLVYVSTDYIFDGTNKGEYLEDDPANPKNEYGRAKWAGEEAVRHSGADYYIVRTSWVFGEFGHN
FVFTMQNLAKTHDKLTVVNDQLGRPTWTRTLAEFMAHLVKTEAASGTYQLSNDNTATWYDFAKEILKNTDVEVAPVTSEQ
FPQKAYRPQHSVMNLDKAKATGYHILTWQEALSEFLESLG
>Mature_279_residues
AKVMIVGANGQLGHELQRLLNEQEIAFDALTEKELDITDLDAVKAKIAELQPAVLYDCAAYTAVDKAEDEGKALNWLVNV
DGTKNLAQVAAEQGVKLVYVSTDYIFDGTNKGEYLEDDPANPKNEYGRAKWAGEEAVRHSGADYYIVRTSWVFGEFGHNF
VFTMQNLAKTHDKLTVVNDQLGRPTWTRTLAEFMAHLVKTEAASGTYQLSNDNTATWYDFAKEILKNTDVEVAPVTSEQF
PQKAYRPQHSVMNLDKAKATGYHILTWQEALSEFLESLG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=283, Percent_Identity=28.9752650176678, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI33519455, Length=283, Percent_Identity=28.9752650176678, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=34.5890410958904, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31277; Mature: 31146

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVMIVGANGQLGHELQRLLNEQEIAFDALTEKELDITDLDAVKAKIAELQPAVLYDCA
CCEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AYTAVDKAEDEGKALNWLVNVDGTKNLAQVAAEQGVKLVYVSTDYIFDGTNKGEYLEDDP
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECEEEECCCCCCCCCCCC
ANPKNEYGRAKWAGEEAVRHSGADYYIVRTSWVFGEFGHNFVFTMQNLAKTHDKLTVVND
CCCHHHCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEHHHHHHHHCCEEEECC
QLGRPTWTRTLAEFMAHLVKTEAASGTYQLSNDNTATWYDFAKEILKNTDVEVAPVTSEQ
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHH
FPQKAYRPQHSVMNLDKAKATGYHILTWQEALSEFLESLG
CCHHHCCCCHHHHCCHHHHCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKVMIVGANGQLGHELQRLLNEQEIAFDALTEKELDITDLDAVKAKIAELQPAVLYDCA
CEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AYTAVDKAEDEGKALNWLVNVDGTKNLAQVAAEQGVKLVYVSTDYIFDGTNKGEYLEDDP
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECEEEECCCCCCCCCCCC
ANPKNEYGRAKWAGEEAVRHSGADYYIVRTSWVFGEFGHNFVFTMQNLAKTHDKLTVVND
CCCHHHCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEHHHHHHHHCCEEEECC
QLGRPTWTRTLAEFMAHLVKTEAASGTYQLSNDNTATWYDFAKEILKNTDVEVAPVTSEQ
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHH
FPQKAYRPQHSVMNLDKAKATGYHILTWQEALSEFLESLG
CCHHHCCCCHHHHCCHHHHCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]