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The map label for this gene is rfbB [H]

Identifier: 28377963

GI number: 28377963

Start: 1086316

End: 1087344

Strand: Direct

Name: rfbB [H]

Synonym: lp_1189

Alternate gene names: 28377963

Gene position: 1086316-1087344 (Clockwise)

Preceding gene: 28377962

Following gene: 28377964

Centisome position: 32.84

GC content: 42.37

Gene sequence:

>1029_bases
ATGGAAAACTTATTAGTTACCGGTGGTGCCGGCTTTATTGGTTCTAACTTTGTTCACTATGTTTATAATCATCATCCCGA
AGTCAAGATTACCGTGTTAGATAAGTTAACTTATGCTGGTAATCGGGCCAACTTGGAAGAAATTCTAGGCGACCGGGTTA
AATTAGTTGTGGGTGACATTTGTGATGCGCCCTTAGTTGATGAATTAATGCAACAAACTGACGCGGTTGTCCATTATGCC
GCTGAAAGCCATAATGATAATTCATTGAAGGATCCATGGCCATTCATCGAAACGAATATTATTGGGACTTATACGCTGAT
TCAATCCGCACATAAGTTTAACAAACGCTTCCACCATGTTTCCACTGATGAAGTATACGGTGATTTACCATTACGGGAAG
ATTTACCGGGCCATGGTGAGGGTGTTGGCGAGAAGTTTACACCAACTAGTCGGTATAAGCCTTCCAGCCCATACTCTTCA
TCTAAGGCCAGCAGTGATCTCTTAGTTCGGGCTTGGGTGCGTTCGTTTGGCTTACAAGCCACTATTTCAAACTGCTCAAA
TAACTATGGCCCTTATCAATATATTGAGAAATTTATTCCACGCCAAATTACCAATATTTTAAGTGGTATTCGGCCGAAAT
TATATGGTAGTGGTAAAAATGTTCGTGACTGGATTCACACGAACGACCATTCTGCGGCCGTTTGGGATATTTTAACCAAG
GGTAAGATTGGCGAGACCTACCTGATTGGTGCCGATGGCGAAATGAACAATAAGGACGTCTTGGAAATGATTCTTGAATT
AATGGGTCAGCCTAAAGATGCTTATGATGTTGTTAAGGATCGTCCCGGCCATGATTTACGTTATGCAATCGATTCAACTA
AGTTGCGCACGGAATTAGGTTGGCAGCCAGAGTTTACTGACTTCCGGTCTGGTTTGAAAGCAACGATTGATTGGTATACG
GACCATCAAGACTGGTGGCAAGCAGATAAGAACAAGGTTGAATCAAACTACGCCAAGAACGGGCAATAA

Upstream 100 bases:

>100_bases
GATCAAGCTATTACTTCTGATAAGGATGCTAAACAACAGACCTTTCAAAGAATTTGAACAGAATAATCCGTTTGTTTACG
GCGAAATTTAAGGAGACGTC

Downstream 100 bases:

>100_bases
AAACACAGAAGATTTTGAATAAGCGGAGTGTCGATTGACAGTTCGCTTATTTTTTAGGAGGAAATGAAAATGGCTAAAGT
AATGATTGTTGGTGCCAATG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MENLLVTGGAGFIGSNFVHYVYNHHPEVKITVLDKLTYAGNRANLEEILGDRVKLVVGDICDAPLVDELMQQTDAVVHYA
AESHNDNSLKDPWPFIETNIIGTYTLIQSAHKFNKRFHHVSTDEVYGDLPLREDLPGHGEGVGEKFTPTSRYKPSSPYSS
SKASSDLLVRAWVRSFGLQATISNCSNNYGPYQYIEKFIPRQITNILSGIRPKLYGSGKNVRDWIHTNDHSAAVWDILTK
GKIGETYLIGADGEMNNKDVLEMILELMGQPKDAYDVVKDRPGHDLRYAIDSTKLRTELGWQPEFTDFRSGLKATIDWYT
DHQDWWQADKNKVESNYAKNGQ

Sequences:

>Translated_342_residues
MENLLVTGGAGFIGSNFVHYVYNHHPEVKITVLDKLTYAGNRANLEEILGDRVKLVVGDICDAPLVDELMQQTDAVVHYA
AESHNDNSLKDPWPFIETNIIGTYTLIQSAHKFNKRFHHVSTDEVYGDLPLREDLPGHGEGVGEKFTPTSRYKPSSPYSS
SKASSDLLVRAWVRSFGLQATISNCSNNYGPYQYIEKFIPRQITNILSGIRPKLYGSGKNVRDWIHTNDHSAAVWDILTK
GKIGETYLIGADGEMNNKDVLEMILELMGQPKDAYDVVKDRPGHDLRYAIDSTKLRTELGWQPEFTDFRSGLKATIDWYT
DHQDWWQADKNKVESNYAKNGQ
>Mature_342_residues
MENLLVTGGAGFIGSNFVHYVYNHHPEVKITVLDKLTYAGNRANLEEILGDRVKLVVGDICDAPLVDELMQQTDAVVHYA
AESHNDNSLKDPWPFIETNIIGTYTLIQSAHKFNKRFHHVSTDEVYGDLPLREDLPGHGEGVGEKFTPTSRYKPSSPYSS
SKASSDLLVRAWVRSFGLQATISNCSNNYGPYQYIEKFIPRQITNILSGIRPKLYGSGKNVRDWIHTNDHSAAVWDILTK
GKIGETYLIGADGEMNNKDVLEMILELMGQPKDAYDVVKDRPGHDLRYAIDSTKLRTELGWQPEFTDFRSGLKATIDWYT
DHQDWWQADKNKVESNYAKNGQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=34.402332361516, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI42516563, Length=332, Percent_Identity=24.0963855421687, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=46.7236467236467, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=47.3684210526316, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=23.6467236467236, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786974, Length=328, Percent_Identity=27.4390243902439, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=35.1515151515151, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=32.2188449848024, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=23.1884057971014, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982038, Length=336, Percent_Identity=25.5952380952381, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=336, Percent_Identity=25.5952380952381, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356223, Length=346, Percent_Identity=26.878612716763, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38669; Mature: 38669

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLLVTGGAGFIGSNFVHYVYNHHPEVKITVLDKLTYAGNRANLEEILGDRVKLVVGDI
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCHHHHHCCHHEEHHHHH
CDAPLVDELMQQTDAVVHYAAESHNDNSLKDPWPFIETNIIGTYTLIQSAHKFNKRFHHV
CCCHHHHHHHHHHHHHEEHHHHCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCC
STDEVYGDLPLREDLPGHGEGVGEKFTPTSRYKPSSPYSSSKASSDLLVRAWVRSFGLQA
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCE
TISNCSNNYGPYQYIEKFIPRQITNILSGIRPKLYGSGKNVRDWIHTNDHSAAVWDILTK
EHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHC
GKIGETYLIGADGEMNNKDVLEMILELMGQPKDAYDVVKDRPGHDLRYAIDSTKLRTELG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCHHHHHHCC
WQPEFTDFRSGLKATIDWYTDHQDWWQADKNKVESNYAKNGQ
CCCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENLLVTGGAGFIGSNFVHYVYNHHPEVKITVLDKLTYAGNRANLEEILGDRVKLVVGDI
CCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCHHHHHCCHHEEHHHHH
CDAPLVDELMQQTDAVVHYAAESHNDNSLKDPWPFIETNIIGTYTLIQSAHKFNKRFHHV
CCCHHHHHHHHHHHHHEEHHHHCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHCCC
STDEVYGDLPLREDLPGHGEGVGEKFTPTSRYKPSSPYSSSKASSDLLVRAWVRSFGLQA
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCE
TISNCSNNYGPYQYIEKFIPRQITNILSGIRPKLYGSGKNVRDWIHTNDHSAAVWDILTK
EHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHC
GKIGETYLIGADGEMNNKDVLEMILELMGQPKDAYDVVKDRPGHDLRYAIDSTKLRTELG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCHHHHHHCC
WQPEFTDFRSGLKATIDWYTDHQDWWQADKNKVESNYAKNGQ
CCCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186; 11796113 [H]