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The map label for this gene is rfbA [H]

Identifier: 28377960

GI number: 28377960

Start: 1083489

End: 1084352

Strand: Direct

Name: rfbA [H]

Synonym: lp_1186

Alternate gene names: 28377960

Gene position: 1083489-1084352 (Clockwise)

Preceding gene: 28377959

Following gene: 28377961

Centisome position: 32.75

GC content: 37.5

Gene sequence:

>864_bases
ATGAAAGGAATTATATTAGCTGGTGGTTCAGGGACTCGTTTATATCCAGTAACAAAGGTTACTTCCAAACAGTTGTTACC
CATTTATGATAAGCCTATGATTTATTATCCACTTTCAACGCTCATGTTGTCAGGAATTAAGGATATATTAATCATTTCCA
CGTCGCGTGATTCGAATAGCTTTAAAGCTTTACTTGGTGATGGTTCAGAACTTGGCATTCATATAGAATATGCAATTCAG
CAAGAACCTAATGGATTAGCGGAAGCCTTTATTATAGGTAAGCAATTTATAGGTGATGATTCGGTTTGTCTGATTCTCGG
GGATAATATTTTTTATGGCAACGGTCTTTCTGAAACTTTACAAAAAGCCGTTCGGTTAAAAGATGGAGCAATTGTTTATG
GTTATAGAGTCAAGGATCCAGAACGTTTTGGCGTTGTAAGTTTTGATAATAAAAAGAATGTGATTAGTATTGAGGAAAAG
CCAACTGATCCTAAAAGTGATTTTGCGGTAACCGGGATATACTTTTTTGATAATGATGTTGTATCTATTGCTGAAAGTAT
TAAACCGTCTGCTCGAGGAGAGCTCGAAATTACGGATATTAATAATGTGTATTTGAAAAGGAAAAAATTGAAGGTCGAAT
TATTAAGTAGGGGCTTTGCCTGGTTGGATACAGGGACGCATGAATCATTGCAAGATGCCTCGTCATTCATTCAAACAGTG
GAACGCCGACAAAATATTTTGATTGCAAGCATTGAAGAGGTCGCTTTTCGTATGGGGTATATTGATAGCAAACAACTGCA
ATCTCTTGCCGCGCCGCTACATAAAACGCGCTATGGACAGTATCTTGCATCACTGGTTGAGTAG

Upstream 100 bases:

>100_bases
TCGAATCTTCGTTTTTCATTGTTGGTATCAACTTTACTGTGATGATGATTTTTTCTAATATGTCTGTCATGCCAAATTCT
AGTTTGGAGGAGTAGTTAGT

Downstream 100 bases:

>100_bases
TTAAGAATGAGTATCCGATAATATGAAAAAAAAAGTTTACACTTGGATATTAATTAGTGTTGTATTGCTTTCTTGCACGG
CTTTGGTCATTGCTAATCAA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKGIILAGGSGTRLYPVTKVTSKQLLPIYDKPMIYYPLSTLMLSGIKDILIISTSRDSNSFKALLGDGSELGIHIEYAIQ
QEPNGLAEAFIIGKQFIGDDSVCLILGDNIFYGNGLSETLQKAVRLKDGAIVYGYRVKDPERFGVVSFDNKKNVISIEEK
PTDPKSDFAVTGIYFFDNDVVSIAESIKPSARGELEITDINNVYLKRKKLKVELLSRGFAWLDTGTHESLQDASSFIQTV
ERRQNILIASIEEVAFRMGYIDSKQLQSLAAPLHKTRYGQYLASLVE

Sequences:

>Translated_287_residues
MKGIILAGGSGTRLYPVTKVTSKQLLPIYDKPMIYYPLSTLMLSGIKDILIISTSRDSNSFKALLGDGSELGIHIEYAIQ
QEPNGLAEAFIIGKQFIGDDSVCLILGDNIFYGNGLSETLQKAVRLKDGAIVYGYRVKDPERFGVVSFDNKKNVISIEEK
PTDPKSDFAVTGIYFFDNDVVSIAESIKPSARGELEITDINNVYLKRKKLKVELLSRGFAWLDTGTHESLQDASSFIQTV
ERRQNILIASIEEVAFRMGYIDSKQLQSLAAPLHKTRYGQYLASLVE
>Mature_287_residues
MKGIILAGGSGTRLYPVTKVTSKQLLPIYDKPMIYYPLSTLMLSGIKDILIISTSRDSNSFKALLGDGSELGIHIEYAIQ
QEPNGLAEAFIIGKQFIGDDSVCLILGDNIFYGNGLSETLQKAVRLKDGAIVYGYRVKDPERFGVVSFDNKKNVISIEEK
PTDPKSDFAVTGIYFFDNDVVSIAESIKPSARGELEITDINNVYLKRKKLKVELLSRGFAWLDTGTHESLQDASSFIQTV
ERRQNILIASIEEVAFRMGYIDSKQLQSLAAPLHKTRYGQYLASLVE

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=286, Percent_Identity=61.5384615384615, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=61.1888111888112, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1788355, Length=261, Percent_Identity=26.0536398467433, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787488, Length=247, Percent_Identity=23.8866396761134, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=25.1366120218579, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355443, Length=250, Percent_Identity=25.2, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24644084, Length=250, Percent_Identity=25.2, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31859; Mature: 31859

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPVTKVTSKQLLPIYDKPMIYYPLSTLMLSGIKDILIISTSRDSNS
CCCEEEECCCCCEEEEEEECCCCCCCEECCCCEEEECHHHHHHCCCCEEEEEEECCCCCC
FKALLGDGSELGIHIEYAIQQEPNGLAEAFIIGKQFIGDDSVCLILGDNIFYGNGLSETL
EEEEECCCCEEEEEEEEEEECCCCCHHHHHEEHHHHCCCCCEEEEEECCEEECCCHHHHH
QKAVRLKDGAIVYGYRVKDPERFGVVSFDNKKNVISIEEKPTDPKSDFAVTGIYFFDNDV
HHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEEEEECCCH
VSIAESIKPSARGELEITDINNVYLKRKKLKVELLSRGFAWLDTGTHESLQDASSFIQTV
HHHHHHCCCCCCCCEEEEECCCEEEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
ERRQNILIASIEEVAFRMGYIDSKQLQSLAAPLHKTRYGQYLASLVE
HHHCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPVTKVTSKQLLPIYDKPMIYYPLSTLMLSGIKDILIISTSRDSNS
CCCEEEECCCCCEEEEEEECCCCCCCEECCCCEEEECHHHHHHCCCCEEEEEEECCCCCC
FKALLGDGSELGIHIEYAIQQEPNGLAEAFIIGKQFIGDDSVCLILGDNIFYGNGLSETL
EEEEECCCCEEEEEEEEEEECCCCCHHHHHEEHHHHCCCCCEEEEEECCEEECCCHHHHH
QKAVRLKDGAIVYGYRVKDPERFGVVSFDNKKNVISIEEKPTDPKSDFAVTGIYFFDNDV
HHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEEEEECCCH
VSIAESIKPSARGELEITDINNVYLKRKKLKVELLSRGFAWLDTGTHESLQDASSFIQTV
HHHHHHCCCCCCCCEEEEECCCEEEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
ERRQNILIASIEEVAFRMGYIDSKQLQSLAAPLHKTRYGQYLASLVE
HHHCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]