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The map label for this gene is ldh

Identifier: 28377890

GI number: 28377890

Start: 999592

End: 1000521

Strand: Direct

Name: ldh

Synonym: lp_1101

Alternate gene names: 28377890

Gene position: 999592-1000521 (Clockwise)

Preceding gene: 28377888

Following gene: 28377891

Centisome position: 30.21

GC content: 47.2

Gene sequence:

>930_bases
ATGGATAAGAAGCAACGCAAAGTCGTAATTGTTGGTGATGGCTCGGTGGGTTCATCATTTGCCTTTTCATTGGTCCAAAA
TTGCGCCCTAGATGAACTCGTTATCGTTGACTTGGTTAAAACGCACGCAGAGGGGGACGTTAAGGATTTGGAAGATGTTG
CCGCCTTTACGAATGCGACCAACATTCATACCGGTGAATATGCGGATGCGCGTGATGCTGACATCGTTGTCATTACGGCT
GGTGTGCCTCGTAAGCCTGGTGAGAGTCGTTTAGATTTGATTAACCGCAATACGAAGATTCTGGAATCCATCGTCAAACC
AGTGGTTGCGAGTGGTTTTAATGGTTGCTTCGTTATCTCAAGTAATCCCGTCGATATTTTGACTTCGATGACGCAACGTT
TATCCGGTTTTCCACGGCATCGGGTCATTGGTACCGGGACTTCCTTGGATACGGCGCGGTTACGGGTCGCCTTGGCTCAG
AAGTTGAATGTTGCCACCACTGCAGTTGATGCTGCGGTACTTGGAGAACATGGTGATAGTTCCATCGTTAATTTTGATGA
AATTATGATCAATGCTCAGCCCTTAAAGACGGTCACAACGGTCGATGATCAGTTCAAAGCTGAAATCGAGCAAGCTGTTC
GTGGTAAAGGTGGTCAAATCATTAGTCAGAAGGGGGCCACGTTCTATGGGGTCGCCGTTAGTTTGATGCAAATCTGCCGA
GCAATTTTGAACGATGAAAATGCTGAGTTGATTGTCTCCGCCGCTTTGTCTGGTCAATATGGCATTAACGATTTGTACTT
GGGGTCACCCGCCATTATTAACCGCAACGGGCTCCAAAAAGTGATCGAAGCTGAGCTATCAGATGATGAGCGTGCCCGGA
TGCAACATTTCGCAGCCAAGATGCTGACCATGATGAATGTGGCATCATAA

Upstream 100 bases:

>100_bases
AAAAATGAAAGCATTTGCGTTATAATACTTGTGAGTTAAGTAACTAAGTAGTTGGAACCACATGTGCAGCACACATTTTT
GATGAAAGAAGGATATTGAC

Downstream 100 bases:

>100_bases
AAATGATGAGTAAGTATGAGGAGGAATTGAGATGACACTAAATAAGGTCAAGTATCGTGATTTTATTGCACCGTTATTGG
TGGGCCTGATCATTTGGTTA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDKKQRKVVIVGDGSVGSSFAFSLVQNCALDELVIVDLVKTHAEGDVKDLEDVAAFTNATNIHTGEYADARDADIVVITA
GVPRKPGESRLDLINRNTKILESIVKPVVASGFNGCFVISSNPVDILTSMTQRLSGFPRHRVIGTGTSLDTARLRVALAQ
KLNVATTAVDAAVLGEHGDSSIVNFDEIMINAQPLKTVTTVDDQFKAEIEQAVRGKGGQIISQKGATFYGVAVSLMQICR
AILNDENAELIVSAALSGQYGINDLYLGSPAIINRNGLQKVIEAELSDDERARMQHFAAKMLTMMNVAS

Sequences:

>Translated_309_residues
MDKKQRKVVIVGDGSVGSSFAFSLVQNCALDELVIVDLVKTHAEGDVKDLEDVAAFTNATNIHTGEYADARDADIVVITA
GVPRKPGESRLDLINRNTKILESIVKPVVASGFNGCFVISSNPVDILTSMTQRLSGFPRHRVIGTGTSLDTARLRVALAQ
KLNVATTAVDAAVLGEHGDSSIVNFDEIMINAQPLKTVTTVDDQFKAEIEQAVRGKGGQIISQKGATFYGVAVSLMQICR
AILNDENAELIVSAALSGQYGINDLYLGSPAIINRNGLQKVIEAELSDDERARMQHFAAKMLTMMNVAS
>Mature_309_residues
MDKKQRKVVIVGDGSVGSSFAFSLVQNCALDELVIVDLVKTHAEGDVKDLEDVAAFTNATNIHTGEYADARDADIVVITA
GVPRKPGESRLDLINRNTKILESIVKPVVASGFNGCFVISSNPVDILTSMTQRLSGFPRHRVIGTGTSLDTARLRVALAQ
KLNVATTAVDAAVLGEHGDSSIVNFDEIMINAQPLKTVTTVDDQFKAEIEQAVRGKGGQIISQKGATFYGVAVSLMQICR
AILNDENAELIVSAALSGQYGINDLYLGSPAIINRNGLQKVIEAELSDDERARMQHFAAKMLTMMNVAS

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI47059044, Length=307, Percent_Identity=34.2019543973941, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI221136809, Length=307, Percent_Identity=34.2019543973941, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI291575128, Length=299, Percent_Identity=34.4481605351171, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI4557032, Length=299, Percent_Identity=34.4481605351171, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI15082234, Length=309, Percent_Identity=33.6569579288026, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI5031857, Length=301, Percent_Identity=34.21926910299, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI260099723, Length=301, Percent_Identity=34.21926910299, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=33.5463258785943, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=33.5463258785943, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI260099725, Length=195, Percent_Identity=38.974358974359, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI260099727, Length=195, Percent_Identity=38.974358974359, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI207028494, Length=300, Percent_Identity=27, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI103472011, Length=292, Percent_Identity=25.3424657534247, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI103472015, Length=184, Percent_Identity=27.7173913043478, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=32.6530612244898, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17554310, Length=288, Percent_Identity=23.9583333333333, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=33.003300330033, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI45550422, Length=296, Percent_Identity=30.0675675675676, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24647881, Length=302, Percent_Identity=25.8278145695364, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH2_LACPL (P59390)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784782.1
- ProteinModelPortal:   P59390
- SMR:   P59390
- GeneID:   1063343
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1101
- NMPDR:   fig|220668.1.peg.916
- HOGENOM:   HBG566126
- OMA:   NCALDEL
- ProtClustDB:   PRK00066
- BioCyc:   LPLA220668:LP_1101-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 33116; Mature: 33116

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 177-177 BINDING 90-90 BINDING 122-122 BINDING 153-153 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKQRKVVIVGDGSVGSSFAFSLVQNCALDELVIVDLVKTHAEGDVKDLEDVAAFTNAT
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
NIHTGEYADARDADIVVITAGVPRKPGESRLDLINRNTKILESIVKPVVASGFNGCFVIS
CCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEC
SNPVDILTSMTQRLSGFPRHRVIGTGTSLDTARLRVALAQKLNVATTAVDAAVLGEHGDS
CCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIVNFDEIMINAQPLKTVTTVDDQFKAEIEQAVRGKGGQIISQKGATFYGVAVSLMQICR
CCEEHHHHEECCCCCCEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHH
AILNDENAELIVSAALSGQYGINDLYLGSPAIINRNGLQKVIEAELSDDERARMQHFAAK
HHHCCCCCEEEEEEECCCCCCCCEEEECCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHH
MLTMMNVAS
HHHHHHCCC
>Mature Secondary Structure
MDKKQRKVVIVGDGSVGSSFAFSLVQNCALDELVIVDLVKTHAEGDVKDLEDVAAFTNAT
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
NIHTGEYADARDADIVVITAGVPRKPGESRLDLINRNTKILESIVKPVVASGFNGCFVIS
CCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEC
SNPVDILTSMTQRLSGFPRHRVIGTGTSLDTARLRVALAQKLNVATTAVDAAVLGEHGDS
CCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SIVNFDEIMINAQPLKTVTTVDDQFKAEIEQAVRGKGGQIISQKGATFYGVAVSLMQICR
CCEEHHHHEECCCCCCEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHH
AILNDENAELIVSAALSGQYGINDLYLGSPAIINRNGLQKVIEAELSDDERARMQHFAAK
HHHCCCCCEEEEEEECCCCCCCCEEEECCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHH
MLTMMNVAS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566