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The map label for this gene is argF

Identifier: 28377418

GI number: 28377418

Start: 481151

End: 482173

Strand: Direct

Name: argF

Synonym: lp_0532

Alternate gene names: 28377418

Gene position: 481151-482173 (Clockwise)

Preceding gene: 28377417

Following gene: 28377419

Centisome position: 14.54

GC content: 47.21

Gene sequence:

>1023_bases
ATGAATAATCAATTTGAAGGTCGGTCATTCTTGAAGGAAATCGACTATACACCAACAGAATTAGCTTACTTAATTGACTT
TGCTAGTCACTTGAAGACCCTTAAAGCACACCATATCCCACACCCTTATTTACAAGGAAAAAATATTGCGTTGCTGTTTG
AAAAAACGTCGACACGGACACGTTCTGCGTTTACTGTGGCGGCTAATGATCTGGGCGCGAATCCAGAGTTTTTGTGTAAA
GACGACATCCAATTCGGGACGAAGGAGTCAGTTGTTGATACGGCGAAAGTACTGGGGTCCATGTATGATGGCATTGAATT
TCGGGGCTTTAAACAAAGCACGGTCGAAGACTTAGCGACCTATAGTGGTGTGCCAGTCTGGAACGGGTTGACCGATGAGT
GGCACCCGACGCAGATTTTAGCGGATTTTTTAACATTAAAGGAACATTTTGGTCATTTAAAAGGGTTGACGTTAGCGTAC
GTTGGTGATGGTCGCAATAATATGGCTAACAGTTTACTTGTGGCGGGAGCGATGCTCGGCATCAATATCGCTATCGGGTC
CCCGGTAGCGTTACAGCCAGCTCCAGCAGTGGTCGCGTTGGCCCAACAGTATGCGCAGGCAGCTGGGAGCACGGTCGTGA
TTACGGCTGATCCGCAACAAGCCGTTACTCAGGCTGATGCGCTTTATACGGATGTCTGGGTCTCAATGGGCGAGCAGGTC
GACTATGGTGAGCGAATCAAGCAACTATTGCCGTTTCAAATTAATACTGCGTTGCTCGCAGCTACTGGCAAGTCATCGAC
GATTGTCATGCATTGCTTACCAGCACTACACGATCTAAAGACGCAGCTAGGAGCCAAATTAGGCGAACAGTATGGAATGA
CGGCGTTTGAAATTACGGATGATGTCTTCCAGAGTAAGCAGGAGGTCGTCTTTGAAGAGGCCGGCAACCGGATGCCAGCA
ATTAAGGCGGTAATGGCGGCTACATTAGGTAACTTATTTATTCCTACCAGCATTTTCAATTAG

Upstream 100 bases:

>100_bases
CGTTTGTTACCCCCAATTGTGATGCAACCGGCCGATTTATTAGCTGGGATTGCCTTGATTGCAAAGGTACTGACAACTTT
GACAACGGAGGTCACGACGA

Downstream 100 bases:

>100_bases
CAAGCTTGCGGGAACTACTAGAGCGTGCTACGATTATATTCGCTTTGTTTCAATCAATACGTGGTTCGTAACCATCCCAC
GTTAAAAAAACTAGGAGGAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQGKNIALLFEKTSTRTRSAFTVAANDLGANPEFLCK
DDIQFGTKESVVDTAKVLGSMYDGIEFRGFKQSTVEDLATYSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAY
VGDGRNNMANSLLVAGAMLGINIAIGSPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAVTQADALYTDVWVSMGEQV
DYGERIKQLLPFQINTALLAATGKSSTIVMHCLPALHDLKTQLGAKLGEQYGMTAFEITDDVFQSKQEVVFEEAGNRMPA
IKAVMAATLGNLFIPTSIFN

Sequences:

>Translated_340_residues
MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQGKNIALLFEKTSTRTRSAFTVAANDLGANPEFLCK
DDIQFGTKESVVDTAKVLGSMYDGIEFRGFKQSTVEDLATYSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAY
VGDGRNNMANSLLVAGAMLGINIAIGSPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAVTQADALYTDVWVSMGEQV
DYGERIKQLLPFQINTALLAATGKSSTIVMHCLPALHDLKTQLGAKLGEQYGMTAFEITDDVFQSKQEVVFEEAGNRMPA
IKAVMAATLGNLFIPTSIFN
>Mature_340_residues
MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQGKNIALLFEKTSTRTRSAFTVAANDLGANPEFLCK
DDIQFGTKESVVDTAKVLGSMYDGIEFRGFKQSTVEDLATYSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAY
VGDGRNNMANSLLVAGAMLGINIAIGSPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAVTQADALYTDVWVSMGEQV
DYGERIKQLLPFQINTALLAATGKSSTIVMHCLPALHDLKTQLGAKLGEQYGMTAFEITDDVFQSKQEVVFEEAGNRMPA
IKAVMAATLGNLFIPTSIFN

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=327, Percent_Identity=37.6146788990826, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI18105007, Length=332, Percent_Identity=24.0963855421687, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=48.6322188449848, Blast_Score=314, Evalue=6e-87,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=47.0948012232416, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI48994908, Length=374, Percent_Identity=24.8663101604278, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367364, Length=306, Percent_Identity=28.4313725490196, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=134, Percent_Identity=35.8208955223881, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=35.0609756097561, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322331, Length=292, Percent_Identity=26.3698630136986, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24642586, Length=244, Percent_Identity=29.9180327868852, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_LACPL (O08322)

Other databases:

- EMBL:   X99978
- EMBL:   AL935253
- RefSeq:   NP_784310.1
- ProteinModelPortal:   O08322
- SMR:   O08322
- GeneID:   1061878
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0532
- NMPDR:   fig|220668.1.peg.444
- HOGENOM:   HBG579429
- OMA:   NHETVES
- ProtClustDB:   PRK02102
- BioCyc:   LPLA220668:LP_0532-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 36975; Mature: 36975

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQGKNIALLFEKTSTRT
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
RSAFTVAANDLGANPEFLCKDDIQFGTKESVVDTAKVLGSMYDGIEFRGFKQSTVEDLAT
CCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAYVGDGRNNMANSLLVAGAMLG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHE
INIAIGSPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAVTQADALYTDVWVSMGEQV
EEEEECCCCEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
DYGERIKQLLPFQINTALLAATGKSSTIVMHCLPALHDLKTQLGAKLGEQYGMTAFEITD
CHHHHHHHHCCCEECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECH
DVFQSKQEVVFEEAGNRMPAIKAVMAATLGNLFIPTSIFN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQGKNIALLFEKTSTRT
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
RSAFTVAANDLGANPEFLCKDDIQFGTKESVVDTAKVLGSMYDGIEFRGFKQSTVEDLAT
CCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
YSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAYVGDGRNNMANSLLVAGAMLG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHE
INIAIGSPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAVTQADALYTDVWVSMGEQV
EEEEECCCCEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
DYGERIKQLLPFQINTALLAATGKSSTIVMHCLPALHDLKTQLGAKLGEQYGMTAFEITD
CHHHHHHHHCCCEECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECH
DVFQSKQEVVFEEAGNRMPAIKAVMAATLGNLFIPTSIFN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098069; 12566566