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The map label for this gene is carB [C]

Identifier: 28377385

GI number: 28377385

Start: 441271

End: 442512

Strand: Direct

Name: carB [C]

Synonym: lp_0493

Alternate gene names: 28377385

Gene position: 441271-442512 (Clockwise)

Preceding gene: 28377384

Following gene: 28377387

Centisome position: 13.34

GC content: 36.88

Gene sequence:

>1242_bases
ATGAAGAAAAGAGTAATTTTAATCGAACCAAGTTTCTATGGAGTTTCCTTTGTCCGAGCTGCTCGAGAATTAGGTTGTGA
AGTTATTTGTGTCGTTAGTGATCAAAAAGATCCCCAAAAATTTGGCTATGAGGGTGAATATGACCAGTTGCTAATTGCGG
ATATTCGTGATAGTGACAGCGTATTAAAAGCCATTCAGAATAGCAAATATAAACGATTTGATGCAATTATTCCAGCCACA
GATTATGCAACGGCAGTGACTGCTAAAGTGGCCGAAAAGTTAAATATGTTTGGCAACTCTTACTTTGCAACTAAATGTGC
CAGAAATAAAGACTTAGCGCGTATTCAGTATGCAAAGAAAGGTGTACCCTCAGCGAAATTTGCAGTAGCAAAAACTATTG
ATGAAGCGCTAGAAGCAAGTAAAAAAATTGGATTTCCATTAATTTTAAAACCAACTAACACTGCTAGTAGTATTGATGTT
TTTTATGTTTCGGATGAACAACAATTACGGGAACGCTTTTTACAAATCTCGCAGTTAAAGCAAAGCTATATGCATTTCAA
AGTACGTGAAGAATACATCTTAGAAGAGTTCATGAGTGGGCCAGAATTTAGTGTAGAGCTTTTCTTGAGTAATAATGAAA
TTGCATTTGTCGAAGTAACAGAAAAGCATACGACTAAACCACCATATTTTGTTGAGTTAATGCATGTTTTTCCAACTACC
GTGGATGTCGCATACAAAAAAGAAATTATCAATACGGCTTATTGTGCAGTTAGGGCGTTAGGGTTCCATAATGGACCAAC
ACATGCTGAGGTTAAATTAACTGATGCCGGTGCCCGTGTCGTGGAAGTAAACGGTCGTCCTGGTGGTGATAATATTACCT
CTGACTTGATTAAGGACGCATACGGCATTGACATCTTCAAAAAAACTGTAGAACTTTATTTAAACAAGACTGTAACGATT
AAACCAACACGACATGGTGCTGCCGCCATTAGTTTTTTGTTTGCGAATAGCAAAGGAAGTTTTGAGTCAGTGCAGGGGTT
AGATAATATCAAAACTACTCAAGGATTCCGGCGACTAGAAATGGAAGTTTCTCCAGGAGATAAGGTTGTACCACCAACTA
ACTCAGATGATAGAATTGGCTATTATATTCTGTCAGGTTCAAATGCTAATAAGTTAAAAGAAACAATTGAAGATCTAAAT
TTAAAAGTACATGTAATAGTAAAGTCTGATTCACGAGTGTAA

Upstream 100 bases:

>100_bases
GGGCTAACATTGGTTTTCTGTGGATGGCCGTGATTATGCTAATTGGCGCTGTATGGAGTTTTATCATGTTAAATACAACA
AAGTTAAGGAGTAAGCAAGT

Downstream 100 bases:

>100_bases
TGCTTTAAAATATGAATTTGTATTCAGTTCGCTGTGACAATTCATGTGAAAGGCTAGTTACTCGTATAGTGTTTAGAAGA
AAGGCTTGATATACGAGCTT

Product: hypothetical protein

Products: ADP; Carbamoyl phosphate; L-Glutamate; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKRVILIEPSFYGVSFVRAARELGCEVICVVSDQKDPQKFGYEGEYDQLLIADIRDSDSVLKAIQNSKYKRFDAIIPAT
DYATAVTAKVAEKLNMFGNSYFATKCARNKDLARIQYAKKGVPSAKFAVAKTIDEALEASKKIGFPLILKPTNTASSIDV
FYVSDEQQLRERFLQISQLKQSYMHFKVREEYILEEFMSGPEFSVELFLSNNEIAFVEVTEKHTTKPPYFVELMHVFPTT
VDVAYKKEIINTAYCAVRALGFHNGPTHAEVKLTDAGARVVEVNGRPGGDNITSDLIKDAYGIDIFKKTVELYLNKTVTI
KPTRHGAAAISFLFANSKGSFESVQGLDNIKTTQGFRRLEMEVSPGDKVVPPTNSDDRIGYYILSGSNANKLKETIEDLN
LKVHVIVKSDSRV

Sequences:

>Translated_413_residues
MKKRVILIEPSFYGVSFVRAARELGCEVICVVSDQKDPQKFGYEGEYDQLLIADIRDSDSVLKAIQNSKYKRFDAIIPAT
DYATAVTAKVAEKLNMFGNSYFATKCARNKDLARIQYAKKGVPSAKFAVAKTIDEALEASKKIGFPLILKPTNTASSIDV
FYVSDEQQLRERFLQISQLKQSYMHFKVREEYILEEFMSGPEFSVELFLSNNEIAFVEVTEKHTTKPPYFVELMHVFPTT
VDVAYKKEIINTAYCAVRALGFHNGPTHAEVKLTDAGARVVEVNGRPGGDNITSDLIKDAYGIDIFKKTVELYLNKTVTI
KPTRHGAAAISFLFANSKGSFESVQGLDNIKTTQGFRRLEMEVSPGDKVVPPTNSDDRIGYYILSGSNANKLKETIEDLN
LKVHVIVKSDSRV
>Mature_413_residues
MKKRVILIEPSFYGVSFVRAARELGCEVICVVSDQKDPQKFGYEGEYDQLLIADIRDSDSVLKAIQNSKYKRFDAIIPAT
DYATAVTAKVAEKLNMFGNSYFATKCARNKDLARIQYAKKGVPSAKFAVAKTIDEALEASKKIGFPLILKPTNTASSIDV
FYVSDEQQLRERFLQISQLKQSYMHFKVREEYILEEFMSGPEFSVELFLSNNEIAFVEVTEKHTTKPPYFVELMHVFPTT
VDVAYKKEIINTAYCAVRALGFHNGPTHAEVKLTDAGARVVEVNGRPGGDNITSDLIKDAYGIDIFKKTVELYLNKTVTI
KPTRHGAAAISFLFANSKGSFESVQGLDNIKTTQGFRRLEMEVSPGDKVVPPTNSDDRIGYYILSGSNANKLKETIEDLN
LKVHVIVKSDSRV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI148839342, Length=287, Percent_Identity=23.3449477351916, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI261245046, Length=284, Percent_Identity=24.2957746478873, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479 [H]

Pfam domain/function: PF02786 CPSase_L_D2 [H]

EC number: 6.3.5.5 [C]

Molecular weight: Translated: 46211; Mature: 46211

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRVILIEPSFYGVSFVRAARELGCEVICVVSDQKDPQKFGYEGEYDQLLIADIRDSDS
CCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCEEEEECCCCHH
VLKAIQNSKYKRFDAIIPATDYATAVTAKVAEKLNMFGNSYFATKCARNKDLARIQYAKK
HHHHHHCCCCEEHHEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHC
GVPSAKFAVAKTIDEALEASKKIGFPLILKPTNTASSIDVFYVSDEQQLRERFLQISQLK
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHH
QSYMHFKVREEYILEEFMSGPEFSVELFLSNNEIAFVEVTEKHTTKPPYFVELMHVFPTT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCCH
VDVAYKKEIINTAYCAVRALGFHNGPTHAEVKLTDAGARVVEVNGRPGGDNITSDLIKDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHH
YGIDIFKKTVELYLNKTVTIKPTRHGAAAISFLFANSKGSFESVQGLDNIKTTQGFRRLE
HCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCHHHCCCEEEE
MEVSPGDKVVPPTNSDDRIGYYILSGSNANKLKETIEDLNLKVHVIVKSDSRV
EEECCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEEEECCCCC
>Mature Secondary Structure
MKKRVILIEPSFYGVSFVRAARELGCEVICVVSDQKDPQKFGYEGEYDQLLIADIRDSDS
CCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCEEEEECCCCHH
VLKAIQNSKYKRFDAIIPATDYATAVTAKVAEKLNMFGNSYFATKCARNKDLARIQYAKK
HHHHHHCCCCEEHHEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHC
GVPSAKFAVAKTIDEALEASKKIGFPLILKPTNTASSIDVFYVSDEQQLRERFLQISQLK
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHH
QSYMHFKVREEYILEEFMSGPEFSVELFLSNNEIAFVEVTEKHTTKPPYFVELMHVFPTT
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCCH
VDVAYKKEIINTAYCAVRALGFHNGPTHAEVKLTDAGARVVEVNGRPGGDNITSDLIKDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHH
YGIDIFKKTVELYLNKTVTIKPTRHGAAAISFLFANSKGSFESVQGLDNIKTTQGFRRLE
HCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCHHHCCCEEEE
MEVSPGDKVVPPTNSDDRIGYYILSGSNANKLKETIEDLNLKVHVIVKSDSRV
EEECCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; GDP; GMP; GTP; IDP; IMP; ITP; N-Acetylglutamate; NEM; Orn; Thiol; XMP [C]

Metal ions: K+; Mg2+; Mn2+; NH4+ [C]

Kcat value (1/min): 120000 [C]

Specific activity: NA

Km value (mM): 93 {NH4+}} 0.38 {L-Gln}} [C]

Substrates: ATP; L-Glutamine; H2O; Bicarbonate [C]

Specific reaction: (2) ATP + L-Glutamine + H2O + Bicarbonate --> (2) ADP + Carbamoyl phosphate + L-Glutamate + (2) H+ + Phosphate [C]

General reaction: Amide group transfer; Amination; Phosphorylation [C]

Inhibitor: 6-Diazo-5-oxon/leucine; Alkyl hydrazines; Azaserine; Guanidine; H2O2; NH2OH; L-2-Amino-4-oxo-5-chloropentanoate; L-Cyanate; NEM; P1, P5-Di (adenosine 5')-pentaphosphate; UDP; UMP phosphate; Urea; UTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]