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The map label for this gene is dus2 [H]

Identifier: 28377345

GI number: 28377345

Start: 397055

End: 398068

Strand: Direct

Name: dus2 [H]

Synonym: lp_0443

Alternate gene names: 28377345

Gene position: 397055-398068 (Clockwise)

Preceding gene: 28377344

Following gene: 28377346

Centisome position: 12.0

GC content: 49.7

Gene sequence:

>1014_bases
GTGACAGCAACACCATTCTGGTCGCAAATTGCGGCCCAAGCCAAGGCAGACGGGCGACCATTTTTTACGATGGCCCCGAT
GGAAGCCGTTAGTAATACTGTTTTTCGGCAAGTCATTAGCCATGCCGCTGCTCCAGATACGTTTTTTAGTGAATTCGTCT
ATGCTAAGAGTATTACGAATCCGAATACGAAGTTTCCGGTGCATGGTCGGTTGTACGTGGCGGCAGCTGAGTCGCAAAAA
CCGGTTGTTCAACTGTGGGGTAACGAAGCGGCTGACTTTGCTTCGGCTACAGCTGAACTTCGTGACCGGGGCTTTGAAGC
AGTTGATATCAATATGGGCTGCCCGGATGGGACGGTCATCAAAAATCATGGTGGCAGCGATTTGATTCGCAATCCGCAGT
GGGCTGAAGACGTGATTGCGGCTGCAAAGACGTCGGGACTGGCCGTTAGTGCGAAGACGCGCTTAGGTTACAGTAAAGTT
GCTGAATATCATGACTGGATTGCAACGTTGTTATCGCAACATGTGGCAGTCTTGACCGTTCACTTGCGGACCAAACAGGA
AATGAGTAAGGTACCCGCACACTTTGAAGTTATCGATGACTTGATTAAAATGCGTGATGCGATCGCCCCGGAAACGTTAC
TCCAGATTAATGGTGACGTGGCTGATTATCAGGCCGGGGTGGCGTTAGCCAAAGCACATCCTGGATTAGATGGTATCATG
ATTGGTCGGGGAGTGTTCGCGAATCCGTTTGCGTTTGAGTCCCAGCCCCAATCGCATGATTTGCATGAACTATTGGGTTT
ACTGAACATGCAGCTCGACTTGTTCGATGACTTTGCCACCCGTTATGACGTGCCACGTTTTCCGTCCTTAAAACGGTTCT
TTAAAATCTATGCGCGCCCAGAACTAGGCGCCACTGACCTGCGTAACACGATGATGGATGCCAAGTCAACGGATGATGTA
CGCAAAATTTTAGCGGCTTATCAAGCCAAAATGCGGGTGACCAATCACAGTTAG

Upstream 100 bases:

>100_bases
TTTAGCCAGCAAACGTCATTCCTGCAAAGCAGGCATATCAATGCTAAAATTAAAAGCATTCAATCCAAAAGTTAAACTAG
TGATAAGGGAGTATGAGAAC

Downstream 100 bases:

>100_bases
TGTCAGCAGCAGACAGGTTGATTAAGGCAGTAATACGTTAATAAACTTCAATACTTATTATATAGAAACATCTCGATATC
GGCAGTGAGTGCTAATGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTATPFWSQIAAQAKADGRPFFTMAPMEAVSNTVFRQVISHAAAPDTFFSEFVYAKSITNPNTKFPVHGRLYVAAAESQK
PVVQLWGNEAADFASATAELRDRGFEAVDINMGCPDGTVIKNHGGSDLIRNPQWAEDVIAAAKTSGLAVSAKTRLGYSKV
AEYHDWIATLLSQHVAVLTVHLRTKQEMSKVPAHFEVIDDLIKMRDAIAPETLLQINGDVADYQAGVALAKAHPGLDGIM
IGRGVFANPFAFESQPQSHDLHELLGLLNMQLDLFDDFATRYDVPRFPSLKRFFKIYARPELGATDLRNTMMDAKSTDDV
RKILAAYQAKMRVTNHS

Sequences:

>Translated_337_residues
MTATPFWSQIAAQAKADGRPFFTMAPMEAVSNTVFRQVISHAAAPDTFFSEFVYAKSITNPNTKFPVHGRLYVAAAESQK
PVVQLWGNEAADFASATAELRDRGFEAVDINMGCPDGTVIKNHGGSDLIRNPQWAEDVIAAAKTSGLAVSAKTRLGYSKV
AEYHDWIATLLSQHVAVLTVHLRTKQEMSKVPAHFEVIDDLIKMRDAIAPETLLQINGDVADYQAGVALAKAHPGLDGIM
IGRGVFANPFAFESQPQSHDLHELLGLLNMQLDLFDDFATRYDVPRFPSLKRFFKIYARPELGATDLRNTMMDAKSTDDV
RKILAAYQAKMRVTNHS
>Mature_336_residues
TATPFWSQIAAQAKADGRPFFTMAPMEAVSNTVFRQVISHAAAPDTFFSEFVYAKSITNPNTKFPVHGRLYVAAAESQKP
VVQLWGNEAADFASATAELRDRGFEAVDINMGCPDGTVIKNHGGSDLIRNPQWAEDVIAAAKTSGLAVSAKTRLGYSKVA
EYHDWIATLLSQHVAVLTVHLRTKQEMSKVPAHFEVIDDLIKMRDAIAPETLLQINGDVADYQAGVALAKAHPGLDGIMI
GRGVFANPFAFESQPQSHDLHELLGLLNMQLDLFDDFATRYDVPRFPSLKRFFKIYARPELGATDLRNTMMDAKSTDDVR
KILAAYQAKMRVTNHS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=29.6610169491525, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693211, Length=244, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=250, Percent_Identity=26.8, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=310, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37156; Mature: 37024

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPFWSQIAAQAKADGRPFFTMAPMEAVSNTVFRQVISHAAAPDTFFSEFVYAKSITN
CCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PNTKFPVHGRLYVAAAESQKPVVQLWGNEAADFASATAELRDRGFEAVDINMGCPDGTVI
CCCCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEE
KNHGGSDLIRNPQWAEDVIAAAKTSGLAVSAKTRLGYSKVAEYHDWIATLLSQHVAVLTV
ECCCCCHHHCCCCHHHHHHHHHHCCCEEEEHHHHCCHHHHHHHHHHHHHHHHCHHHEEEE
HLRTKQEMSKVPAHFEVIDDLIKMRDAIAPETLLQINGDVADYQAGVALAKAHPGLDGIM
EHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHCCHHHHCCCCCCEEE
IGRGVFANPFAFESQPQSHDLHELLGLLNMQLDLFDDFATRYDVPRFPSLKRFFKIYARP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
ELGATDLRNTMMDAKSTDDVRKILAAYQAKMRVTNHS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TATPFWSQIAAQAKADGRPFFTMAPMEAVSNTVFRQVISHAAAPDTFFSEFVYAKSITN
CCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PNTKFPVHGRLYVAAAESQKPVVQLWGNEAADFASATAELRDRGFEAVDINMGCPDGTVI
CCCCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEE
KNHGGSDLIRNPQWAEDVIAAAKTSGLAVSAKTRLGYSKVAEYHDWIATLLSQHVAVLTV
ECCCCCHHHCCCCHHHHHHHHHHCCCEEEEHHHHCCHHHHHHHHHHHHHHHHCHHHEEEE
HLRTKQEMSKVPAHFEVIDDLIKMRDAIAPETLLQINGDVADYQAGVALAKAHPGLDGIM
EHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHEEECCCHHHHHHCCHHHHCCCCCCEEE
IGRGVFANPFAFESQPQSHDLHELLGLLNMQLDLFDDFATRYDVPRFPSLKRFFKIYARP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
ELGATDLRNTMMDAKSTDDVRKILAAYQAKMRVTNHS
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]