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The map label for this gene is choS [H]

Identifier: 28377282

GI number: 28377282

Start: 334512

End: 336038

Strand: Direct

Name: choS [H]

Synonym: lp_0367

Alternate gene names: 28377282

Gene position: 334512-336038 (Clockwise)

Preceding gene: 28377280

Following gene: 28377283

Centisome position: 10.11

GC content: 44.99

Gene sequence:

>1527_bases
ATGCAGACATTAATACAGACGTTTATAGATCGACGCGGGGATTTGCTGACTGCGCTATGGCAACACTTGGGGATTTCATT
AGCATCATTAGTCATCGCAATGGTAATTGCGATTCCGTTGGCTATTTGGGTCGTTCGACGACCACGGTGGGCCGAGGGAT
TGTTACAGCTCACGAGTGTCCTACAGACGATTCCGTCTTTGGCACTGTTAGGGTTATTGATTCCGTTAGTTGGGATTGGA
ACGGTGCCAGCAGTAATTGCGCTGGTGATCTATGCTTTACTGCCGATTTTTCAAAATACTTACTTGGGTATCTCAGAAAT
CGATGCCTCAATTGAAGAGGCCGCCGATGCCTTTGGGATGTCACGAATGCGTAAGTTGTTTAAAGTTGAACTACCCATTG
CCCTACCACAGATCATTGCTGGGATTCGGACCGCGCTCGTTTTAATCATTGGGACGGCTACTTTGGCCGCTTTGATTGGT
GCTGGGGGTCTCGGGACCTTTATCATGCTCGGTATTGACCGTAATGATACTTCGTTATTATTGATTGGGGCCATCTCATC
AGCATTGTTAGCAATTCTGCTGAGTGCGCTCGTTCGGTGGTTTCAAACGGCTAAACCACGCCACGCCTTAATCGTCTTTG
TCGGTATTTTAGCTTTACTTGGTGGTGGCGGGGCTTATAGTGTCTATGCCAATCGAGTTGAAACAATTACGATTGCAGGT
AAACTTGGTTCCGAACCAGAAATCTTGATTAATATGTATAAGCAGCTGATTGAAGCTGAAGATGAACACGTTCATGTGAC
GCTCAAGCCTAACTTTGGCAAGACCACGTTCTTATTCAGCGCGTTAAAGAATAATCAGGTTGATATTTATCCTGAATTTA
CTGGCTCGGTGCTGGAGACCTTAGTTAAGGGAAATAACCCAGCTGGTCAAACAGCTAACCAGACCTATCAGCTCGCCAAA
CAGCGCCTCGCTAAGCAGGAACAAATGACTTACTTGAAGCCGATGCAGTATAACAATACGTACGCATTGGCAGTGACTAA
GAAATTTCAACAAGAACATCATTTGAAGACAATCAGCGACTTAACGCAAGTTGAATCGATTCTGAAACCCGGAATGACCC
TAGAGTTTATTGATCGTAATGATGGCTTAAAAGGAATCAAGAAGACTTATGGGTTAGACGTGACTGCCAAGTCGATGGAG
CCGGCGCTACGTTATGAAGCCATCAGTAAGGGGAAAATCAACTTGGTAGATGCCTATGCGACGGATAGTGAATTACGGCA
GTATCACTTGGCCTTATTGAAGGATAACAAGCACTTCTTCCCAACGTATCAAGGGGCACCGTTGATGAAGACGAGCTTTG
CCAACAAACATCCTAAGGTCGTTAAAGCGTTGAATAAGTTAGCAGGAAAGATTTCAGAAACTGATATGCAAGAAATGAAC
TATGAAGTCAATGTTAAGAAGCAGTCCGCTTCGACGGTTGCACATCGCTATCTTGTGAAGCACGGTTTATTGAAGGAGGG
ACGTTAA

Upstream 100 bases:

>100_bases
TCAGTGCGTATTACTATGATTTTTGGCGGATTATGCTACGATTAAATACATGGATAATTAAGAAGTTATTAAGCAATAAA
GGTGAGCACTGGAGGTTAGC

Downstream 100 bases:

>100_bases
ATGACAACGGCAATTGAATTTCAACACGTCCAGAAAGACTTTAATGGGCAGACCGTGATTCCCGACCTTAATTTAACGAT
TGACCAGGGTGAGCTATTTG

Product: glycine betaine/carnitine/choline ABC transporter, substrate binding and permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: Osmoprotectant-binding protein [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSVLQTIPSLALLGLLIPLVGIG
TVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGMSRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIG
AGGLGTFIMLGIDRNDTSLLLIGAISSALLAILLSALVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAG
KLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLETLVKGNNPAGQTANQTYQLAK
QRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISDLTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSME
PALRYEAISKGKINLVDAYATDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMN
YEVNVKKQSASTVAHRYLVKHGLLKEGR

Sequences:

>Translated_508_residues
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSVLQTIPSLALLGLLIPLVGIG
TVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGMSRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIG
AGGLGTFIMLGIDRNDTSLLLIGAISSALLAILLSALVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAG
KLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLETLVKGNNPAGQTANQTYQLAK
QRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISDLTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSME
PALRYEAISKGKINLVDAYATDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMN
YEVNVKKQSASTVAHRYLVKHGLLKEGR
>Mature_508_residues
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSVLQTIPSLALLGLLIPLVGIG
TVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGMSRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIG
AGGLGTFIMLGIDRNDTSLLLIGAISSALLAILLSALVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAG
KLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLETLVKGNNPAGQTANQTYQLAK
QRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISDLTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSME
PALRYEAISKGKINLVDAYATDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMN
YEVNVKKQSASTVAHRYLVKHGLLKEGR

Specific function: Member of a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine, and choline [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789033, Length=167, Percent_Identity=40.1197604790419, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788451, Length=211, Percent_Identity=36.9668246445498, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788449, Length=168, Percent_Identity=42.8571428571429, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788452, Length=307, Percent_Identity=25.4071661237785, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081802, Length=144, Percent_Identity=35.4166666666667, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007210 [H]

Pfam domain/function: PF04069 OpuAC [H]

EC number: NA

Molecular weight: Translated: 55811; Mature: 55811

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LQTIPSLALLGLLIPLVGIGTVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIGAGGLGTFIMLGIDRNDTSLL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEE
LIGAISSALLAILLSALVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAG
EHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEEEC
KLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLET
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHH
LVKGNNPAGQTANQTYQLAKQRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
LTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSMEPALRYEAISKGKINLVDAYA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEHHHCCHHHHHHHHCCCCEEEEEECC
TDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCC
YEVNVKKQSASTVAHRYLVKHGLLKEGR
CEEEEEHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure
MQTLIQTFIDRRGDLLTALWQHLGISLASLVIAMVIAIPLAIWVVRRPRWAEGLLQLTSV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LQTIPSLALLGLLIPLVGIGTVPAVIALVIYALLPIFQNTYLGISEIDASIEEAADAFGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SRMRKLFKVELPIALPQIIAGIRTALVLIIGTATLAALIGAGGLGTFIMLGIDRNDTSLL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEE
LIGAISSALLAILLSALVRWFQTAKPRHALIVFVGILALLGGGGAYSVYANRVETITIAG
EHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEEEC
KLGSEPEILINMYKQLIEAEDEHVHVTLKPNFGKTTFLFSALKNNQVDIYPEFTGSVLET
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHH
LVKGNNPAGQTANQTYQLAKQRLAKQEQMTYLKPMQYNNTYALAVTKKFQQEHHLKTISD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
LTQVESILKPGMTLEFIDRNDGLKGIKKTYGLDVTAKSMEPALRYEAISKGKINLVDAYA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEHHHCCHHHHHHHHCCCCEEEEEECC
TDSELRQYHLALLKDNKHFFPTYQGAPLMKTSFANKHPKVVKALNKLAGKISETDMQEMN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCC
YEVNVKKQSASTVAHRYLVKHGLLKEGR
CEEEEEHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]