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The map label for this gene is mtnE [H]

Identifier: 28377259

GI number: 28377259

Start: 310326

End: 311501

Strand: Direct

Name: mtnE [H]

Synonym: lp_0339

Alternate gene names: 28377259

Gene position: 310326-311501 (Clockwise)

Preceding gene: 28377258

Following gene: 28377261

Centisome position: 9.38

GC content: 48.64

Gene sequence:

>1176_bases
TTGAAATTTGAAGAGTCCAAAATTTTGCAGCAATTACCAAAACAGTTTTTTGCGAATCTGGTCAAAAAGGTCAATGCCAA
AGCGGCGGCCGGTGCGGATGTGATTAATTTAGGTCAAGGCAATCCTGACCAACCAACCCCTGAGTTTGTTGTTAAAGCAA
TGCAGACTGCAACTGCCAATCCCGCGGATCACAAGTATTCGTTGTTTCGAGGTTTGCCGCGATTCAAACAAGCCGCGGCT
GATTTTTACGAACGAGAGTACGGTGTGAAATTAGACCCGGAGACCGAGATTGCGGTTTTGGGTGGCAGTAAGATTGGGTT
GGTCGAGTTACCCTTTGCCTTACTAAATCCGGGCGATACAATGATTTTACCTGACCCCGGTTACCCAGACTATTTATCTG
GTATTACGTTGGCACAGGTGAAGTTGGCTTTACTACGGTTAACAGCGGCCAATCATTTCTTACCGGATTATCAGCAGGTT
GATCCACAAGTGGCTGCGGCGGCCAAGCTGTTGTACCTGAATTACCCGAATAATCCCACTGGTGCTGTTGCAACATCGCA
GTTTTTTCAAGACACGGTGGATTTTGCAAATGAACATCAGATTGGTATCGTTCATGACTTTGCTTATGGTGCAATTGGGT
TCGATGGTCAGCGACCAGTGAGCTTCCTACAGACACCGGGGGCTAAAGACGTTGGAATTGAGATGTACACGTTATCTAAG
TCCTTTAACATGGCAGGTTGGCGCGTTGGTTTTGCCGCTGGTAATGCTGACATGATTGAAGCCTTAAATTTGATTCAGGA
TCATCTGTTTGTCAGCGTCTTTCCGGCCATTCAAGATGCAGCGATTGCAGCCTTGAACAGTGACCAACAGACGGTCCACG
AACTGGTTAGACTGTATGAACGACGCCGTAATCAGTTCTTTACGACGGCCCGCAAGATTGGGTGGGAGCCCTACCCATCA
GGGGGCTCATTTTACGCTTGGATGCCAGTCCCTGAAGGGTATACCAGTGAAGCATTTGCCGATTTGCTGTTGGATAAAGC
GGCGGTTGCGGTGGCGCCAGGAAACGGCTTTGGTGCGGGTGGCGAAGGCTATGTGCGCGTTGGACTCTTGATTGATGAAC
CCCGCTTCACGGAAGCCTGTCAGCGAATTGCAAAACTGCACTTATTCGATCGTTAG

Upstream 100 bases:

>100_bases
AAAATTGACATATTCTAATTGAAAACTCATAATTAGGCTTGAGATTATGGTTGATTGTAAATTAGTACTGACAACCATTG
ACAGAAGGGAAGCACTAATT

Downstream 100 bases:

>100_bases
GATGACCTCTTAGCATTAATGATGATAGCCAATCACTTACTGAGCATAATTCATGCAACGGGGGCGCAACTCGTTCTTGA
TTGCTCGGAAATATTGAGCT

Product: transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKFEESKILQQLPKQFFANLVKKVNAKAAAGADVINLGQGNPDQPTPEFVVKAMQTATANPADHKYSLFRGLPRFKQAAA
DFYEREYGVKLDPETEIAVLGGSKIGLVELPFALLNPGDTMILPDPGYPDYLSGITLAQVKLALLRLTAANHFLPDYQQV
DPQVAAAAKLLYLNYPNNPTGAVATSQFFQDTVDFANEHQIGIVHDFAYGAIGFDGQRPVSFLQTPGAKDVGIEMYTLSK
SFNMAGWRVGFAAGNADMIEALNLIQDHLFVSVFPAIQDAAIAALNSDQQTVHELVRLYERRRNQFFTTARKIGWEPYPS
GGSFYAWMPVPEGYTSEAFADLLLDKAAVAVAPGNGFGAGGEGYVRVGLLIDEPRFTEACQRIAKLHLFDR

Sequences:

>Translated_391_residues
MKFEESKILQQLPKQFFANLVKKVNAKAAAGADVINLGQGNPDQPTPEFVVKAMQTATANPADHKYSLFRGLPRFKQAAA
DFYEREYGVKLDPETEIAVLGGSKIGLVELPFALLNPGDTMILPDPGYPDYLSGITLAQVKLALLRLTAANHFLPDYQQV
DPQVAAAAKLLYLNYPNNPTGAVATSQFFQDTVDFANEHQIGIVHDFAYGAIGFDGQRPVSFLQTPGAKDVGIEMYTLSK
SFNMAGWRVGFAAGNADMIEALNLIQDHLFVSVFPAIQDAAIAALNSDQQTVHELVRLYERRRNQFFTTARKIGWEPYPS
GGSFYAWMPVPEGYTSEAFADLLLDKAAVAVAPGNGFGAGGEGYVRVGLLIDEPRFTEACQRIAKLHLFDR
>Mature_391_residues
MKFEESKILQQLPKQFFANLVKKVNAKAAAGADVINLGQGNPDQPTPEFVVKAMQTATANPADHKYSLFRGLPRFKQAAA
DFYEREYGVKLDPETEIAVLGGSKIGLVELPFALLNPGDTMILPDPGYPDYLSGITLAQVKLALLRLTAANHFLPDYQQV
DPQVAAAAKLLYLNYPNNPTGAVATSQFFQDTVDFANEHQIGIVHDFAYGAIGFDGQRPVSFLQTPGAKDVGIEMYTLSK
SFNMAGWRVGFAAGNADMIEALNLIQDHLFVSVFPAIQDAAIAALNSDQQTVHELVRLYERRRNQFFTTARKIGWEPYPS
GGSFYAWMPVPEGYTSEAFADLLLDKAAVAVAPGNGFGAGGEGYVRVGLLIDEPRFTEACQRIAKLHLFDR

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713256, Length=341, Percent_Identity=23.7536656891496, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI56713254, Length=341, Percent_Identity=23.7536656891496, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI95147551, Length=321, Percent_Identity=24.9221183800623, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI169881279, Length=321, Percent_Identity=24.9221183800623, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788722, Length=385, Percent_Identity=36.3636363636364, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1786816, Length=356, Percent_Identity=25, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788627, Length=320, Percent_Identity=26.25, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71994472, Length=269, Percent_Identity=27.5092936802974, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71994476, Length=269, Percent_Identity=27.5092936802974, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17567369, Length=271, Percent_Identity=23.6162361623616, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322401, Length=390, Percent_Identity=22.8205128205128, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28573069, Length=386, Percent_Identity=23.3160621761658, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646114, Length=386, Percent_Identity=23.3160621761658, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573067, Length=386, Percent_Identity=23.3160621761658, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573065, Length=386, Percent_Identity=23.3160621761658, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.- [C]

Molecular weight: Translated: 42801; Mature: 42801

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEESKILQQLPKQFFANLVKKVNAKAAAGADVINLGQGNPDQPTPEFVVKAMQTATAN
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCC
PADHKYSLFRGLPRFKQAAADFYEREYGVKLDPETEIAVLGGSKIGLVELPFALLNPGDT
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCEEEECCCEECCEECCHHHCCCCCE
MILPDPGYPDYLSGITLAQVKLALLRLTAANHFLPDYQQVDPQVAAAAKLLYLNYPNNPT
EEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHEEEEEEECCCCCC
GAVATSQFFQDTVDFANEHQIGIVHDFAYGAIGFDGQRPVSFLQTPGAKDVGIEMYTLSK
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECC
SFNMAGWRVGFAAGNADMIEALNLIQDHLFVSVFPAIQDAAIAALNSDQQTVHELVRLYE
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RRRNQFFTTARKIGWEPYPSGGSFYAWMPVPEGYTSEAFADLLLDKAAVAVAPGNGFGAG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCC
GEGYVRVGLLIDEPRFTEACQRIAKLHLFDR
CCCEEEEEEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFEESKILQQLPKQFFANLVKKVNAKAAAGADVINLGQGNPDQPTPEFVVKAMQTATAN
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCC
PADHKYSLFRGLPRFKQAAADFYEREYGVKLDPETEIAVLGGSKIGLVELPFALLNPGDT
CCCCHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCEEEECCCEECCEECCHHHCCCCCE
MILPDPGYPDYLSGITLAQVKLALLRLTAANHFLPDYQQVDPQVAAAAKLLYLNYPNNPT
EEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHEEEEEEECCCCCC
GAVATSQFFQDTVDFANEHQIGIVHDFAYGAIGFDGQRPVSFLQTPGAKDVGIEMYTLSK
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECC
SFNMAGWRVGFAAGNADMIEALNLIQDHLFVSVFPAIQDAAIAALNSDQQTVHELVRLYE
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RRRNQFFTTARKIGWEPYPSGGSFYAWMPVPEGYTSEAFADLLLDKAAVAVAPGNGFGAG
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCC
GEGYVRVGLLIDEPRFTEACQRIAKLHLFDR
CCCEEEEEEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]