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The map label for this gene is ack2 [H]

Identifier: 28377232

GI number: 28377232

Start: 286486

End: 287682

Strand: Direct

Name: ack2 [H]

Synonym: lp_0310

Alternate gene names: 28377232

Gene position: 286486-287682 (Clockwise)

Preceding gene: 28377222

Following gene: 28377234

Centisome position: 8.66

GC content: 42.69

Gene sequence:

>1197_bases
ATGGCAAAAATTTTAGCAATTAACGCCGGCAGTTCAACTTTAAAATGGAAGCTTTTCAGTGTTCCCGAAGAAACGGTAAT
CGCATCGGGAATGGTTGATCGGCTGGGATTGCCAGATTCCGTTTTCACAATTAAGAAGGCGGATGGTAGTAAATATTCTG
AAACTAAGGACCAAATTGATGCAAAAGAAGCCGCTGCAATGGTCTTAACACGGCTGAAGAGCGATAATATTGTGACTCAT
CTTAGTGAAATCACTGGGGTTGGTCATCGGGTTGTTGCGGGTGGCGAAGATTTCAAAGACTCTGTCGTTATTACTTCGCA
AACCTTGAAAAAAATCAAAGACTTATCTGAGTATGCGCCGCTACATAATCCAACGCAGGCTTATTATATTGAAGTATTCC
GTGATTTATTACCAAAAGCGACTCAAGTAGCCGTGTTTGACACTTCGCTGTATGCGGATATGCCTAAAGTTAATTACCTA
TATAGTATTCCTTACGATTATTATGAGAAATTTGGCGCGCGGAAGTATGGGGCACATGGCACAAGTCATCGTTACGTTGC
TAATCGGACGGCCCAACTATTGGGGAAACCATTGGATTCGCTTAAATTAGTGACATTACATTTAGGGTCGGGTGCTTCGA
TCACGGCTTTTGATCATGGTAAAGCCGTCGACACTTCAATGGGCTTTACGCCGCTAGCTGGGATTACCATGAGTACCCGT
TCCGGTGATATTGATGCCTCAATTATTCCATTCTTGATGCGCCATTTAGGGATCTCTGATGTGGAAGACTTTGTTGATAT
TTTGAATCATAAGTCAGGGTTACTTGGTATTTCAGGTTTGTCACCTGATATGCGTGATCTTGATAAGACGCAAGATACCC
GTGAACGGTCAAAATTAGCAATTGATATTTTTGTTAACCGGGTTGTGAAGTATGTCGGCAGCTATATTGCTGAAATGAAC
GGGATTGATGCCCTTGTCTTCACGGCTGGTGTGGGTGAAAACTCATGGATGATCCGCGAACGTGTCACTAAAGCCTTAGG
ATACTTTGGCGTGACAGTGGATGATGAAAAGAATCATATGCACGGTAAGGAACAAATTATTAGCAAAGATGACGAACCGG
TAACGGTCATGGTTGTCCCAACTAATGAAGAGTTGATGATTGTGCGTGACGTTGAGCGGTTATCTAAAGCTGAATAA

Upstream 100 bases:

>100_bases
TATGAAAATTGAAAGTTTGTGAACAAATCCTTGAAAGCGTATCCACTATTAGTTAAACTGGCATTAGGCACACTTGATGA
TGGATGGAGGAAAAATAGTC

Downstream 100 bases:

>100_bases
ATGAGTCGATTAAAAACGCGTGATTCGGATTGTTGTCCGAGTCACGCGTTTTGAGTTTTGGATTCTAATCAGCATAGCGC
TAAGTCAACTCTTGTCCCCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKILAINAGSSTLKWKLFSVPEETVIASGMVDRLGLPDSVFTIKKADGSKYSETKDQIDAKEAAAMVLTRLKSDNIVTH
LSEITGVGHRVVAGGEDFKDSVVITSQTLKKIKDLSEYAPLHNPTQAYYIEVFRDLLPKATQVAVFDTSLYADMPKVNYL
YSIPYDYYEKFGARKYGAHGTSHRYVANRTAQLLGKPLDSLKLVTLHLGSGASITAFDHGKAVDTSMGFTPLAGITMSTR
SGDIDASIIPFLMRHLGISDVEDFVDILNHKSGLLGISGLSPDMRDLDKTQDTRERSKLAIDIFVNRVVKYVGSYIAEMN
GIDALVFTAGVGENSWMIRERVTKALGYFGVTVDDEKNHMHGKEQIISKDDEPVTVMVVPTNEELMIVRDVERLSKAE

Sequences:

>Translated_398_residues
MAKILAINAGSSTLKWKLFSVPEETVIASGMVDRLGLPDSVFTIKKADGSKYSETKDQIDAKEAAAMVLTRLKSDNIVTH
LSEITGVGHRVVAGGEDFKDSVVITSQTLKKIKDLSEYAPLHNPTQAYYIEVFRDLLPKATQVAVFDTSLYADMPKVNYL
YSIPYDYYEKFGARKYGAHGTSHRYVANRTAQLLGKPLDSLKLVTLHLGSGASITAFDHGKAVDTSMGFTPLAGITMSTR
SGDIDASIIPFLMRHLGISDVEDFVDILNHKSGLLGISGLSPDMRDLDKTQDTRERSKLAIDIFVNRVVKYVGSYIAEMN
GIDALVFTAGVGENSWMIRERVTKALGYFGVTVDDEKNHMHGKEQIISKDDEPVTVMVVPTNEELMIVRDVERLSKAE
>Mature_397_residues
AKILAINAGSSTLKWKLFSVPEETVIASGMVDRLGLPDSVFTIKKADGSKYSETKDQIDAKEAAAMVLTRLKSDNIVTHL
SEITGVGHRVVAGGEDFKDSVVITSQTLKKIKDLSEYAPLHNPTQAYYIEVFRDLLPKATQVAVFDTSLYADMPKVNYLY
SIPYDYYEKFGARKYGAHGTSHRYVANRTAQLLGKPLDSLKLVTLHLGSGASITAFDHGKAVDTSMGFTPLAGITMSTRS
GDIDASIIPFLMRHLGISDVEDFVDILNHKSGLLGISGLSPDMRDLDKTQDTRERSKLAIDIFVNRVVKYVGSYIAEMNG
IDALVFTAGVGENSWMIRERVTKALGYFGVTVDDEKNHMHGKEQIISKDDEPVTVMVVPTNEELMIVRDVERLSKAE

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=42.14463840399, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=38.6363636363636, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43849; Mature: 43717

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILAINAGSSTLKWKLFSVPEETVIASGMVDRLGLPDSVFTIKKADGSKYSETKDQID
CCEEEEEECCCCEEEEEEECCCHHHHHHHCCHHHCCCCCCEEEEEECCCCCCHHHHHHHH
AKEAAAMVLTRLKSDNIVTHLSEITGVGHRVVAGGEDFKDSVVITSQTLKKIKDLSEYAP
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHCC
LHNPTQAYYIEVFRDLLPKATQVAVFDTSLYADMPKVNYLYSIPYDYYEKFGARKYGAHG
CCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCC
TSHRYVANRTAQLLGKPLDSLKLVTLHLGSGASITAFDHGKAVDTSMGFTPLAGITMSTR
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCEECCCCCCCCCCCEEEECC
SGDIDASIIPFLMRHLGISDVEDFVDILNHKSGLLGISGLSPDMRDLDKTQDTRERSKLA
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
IDIFVNRVVKYVGSYIAEMNGIDALVFTAGVGENSWMIRERVTKALGYFGVTVDDEKNHM
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCC
HGKEQIISKDDEPVTVMVVPTNEELMIVRDVERLSKAE
CCHHHHHCCCCCCEEEEEEECCCCEEEEEEHHHHHCCC
>Mature Secondary Structure 
AKILAINAGSSTLKWKLFSVPEETVIASGMVDRLGLPDSVFTIKKADGSKYSETKDQID
CEEEEEECCCCEEEEEEECCCHHHHHHHCCHHHCCCCCCEEEEEECCCCCCHHHHHHHH
AKEAAAMVLTRLKSDNIVTHLSEITGVGHRVVAGGEDFKDSVVITSQTLKKIKDLSEYAP
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHCC
LHNPTQAYYIEVFRDLLPKATQVAVFDTSLYADMPKVNYLYSIPYDYYEKFGARKYGAHG
CCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCC
TSHRYVANRTAQLLGKPLDSLKLVTLHLGSGASITAFDHGKAVDTSMGFTPLAGITMSTR
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCEECCCCCCCCCCCEEEECC
SGDIDASIIPFLMRHLGISDVEDFVDILNHKSGLLGISGLSPDMRDLDKTQDTRERSKLA
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
IDIFVNRVVKYVGSYIAEMNGIDALVFTAGVGENSWMIRERVTKALGYFGVTVDDEKNHM
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCC
HGKEQIISKDDEPVTVMVVPTNEELMIVRDVERLSKAE
CCHHHHHCCCCCCEEEEEEECCCCEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10941799 [H]