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The map label for this gene is tag1 [H]

Identifier: 28377219

GI number: 28377219

Start: 271075

End: 271638

Strand: Direct

Name: tag1 [H]

Synonym: lp_0296

Alternate gene names: 28377219

Gene position: 271075-271638 (Clockwise)

Preceding gene: 28377218

Following gene: 28377220

Centisome position: 8.19

GC content: 49.82

Gene sequence:

>564_bases
ATGCAACGATGTGCATGGGCGGATTCATCACCAGCAATGCAAGCGTATCATGACAACGAATGGGGGCGGCCGCAACATAA
TCAGCAACGACTTTTTGAACTGTTGTGCTTGGAGACTTATCAAGCAGGACTGAGCTGGCAGACCGTCCTGAATAAGCGGG
CGGCTTTTAATGAAGATTTTGCGGATTACGATGTTGACCAGGTGGCCGCTATGACGACCGCCGATGTTGAGCGACTACTA
ACCGATGCTCGAATTATTCGGCATCGTCAGAAGTTAATGGCAACGATCAACAATGCTCGTGTGATTCGAGATTGGCCGGC
CGGGGAAGACTTTGCCACTTGGTTATGGGCGTTTGTTGATAACCAGCCCATACGCCAGGCGTGGGCGTCAATCGATGAGG
TTCCAGCAACAAACGACTTGGCGCAACAAGTGTCTAAAGAGATGAAACGCCGCGGCTTCAAGTTTGTCGGACCGACAACC
ATTTATTCTTATCTTCAAGCGACTGGTCTCATTAATGATCATGTGGTGGACTGCCCATTTGCTCCAGAAAATTTACCGGC
CTAA

Upstream 100 bases:

>100_bases
GCTGCACTTAGATTTTAAAAACAGTGTTAGTTTTAGTCTAAGAGTATGCGGCGAGTATGAGATAGCCGGATTGGATAGTG
GCGATAAGGAGATGTTTTTA

Downstream 100 bases:

>100_bases
CGCGCTTAATTCCTGGCGCGGTGAGTAAGTGAGCCGTCAATTTAAAACCAGCGCGATGACAACTGTCAGGCGCTGGTTTT
TATTAAAATCAATATGTTTT

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MQRCAWADSSPAMQAYHDNEWGRPQHNQQRLFELLCLETYQAGLSWQTVLNKRAAFNEDFADYDVDQVAAMTTADVERLL
TDARIIRHRQKLMATINNARVIRDWPAGEDFATWLWAFVDNQPIRQAWASIDEVPATNDLAQQVSKEMKRRGFKFVGPTT
IYSYLQATGLINDHVVDCPFAPENLPA

Sequences:

>Translated_187_residues
MQRCAWADSSPAMQAYHDNEWGRPQHNQQRLFELLCLETYQAGLSWQTVLNKRAAFNEDFADYDVDQVAAMTTADVERLL
TDARIIRHRQKLMATINNARVIRDWPAGEDFATWLWAFVDNQPIRQAWASIDEVPATNDLAQQVSKEMKRRGFKFVGPTT
IYSYLQATGLINDHVVDCPFAPENLPA
>Mature_187_residues
MQRCAWADSSPAMQAYHDNEWGRPQHNQQRLFELLCLETYQAGLSWQTVLNKRAAFNEDFADYDVDQVAAMTTADVERLL
TDARIIRHRQKLMATINNARVIRDWPAGEDFATWLWAFVDNQPIRQAWASIDEVPATNDLAQQVSKEMKRRGFKFVGPTT
IYSYLQATGLINDHVVDCPFAPENLPA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=187, Percent_Identity=48.6631016042781, Blast_Score=192, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21460; Mature: 21460

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRCAWADSSPAMQAYHDNEWGRPQHNQQRLFELLCLETYQAGLSWQTVLNKRAAFNEDF
CCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
ADYDVDQVAAMTTADVERLLTDARIIRHRQKLMATINNARVIRDWPAGEDFATWLWAFVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHC
NQPIRQAWASIDEVPATNDLAQQVSKEMKRRGFKFVGPTTIYSYLQATGLINDHVVDCPF
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCC
APENLPA
CCCCCCC
>Mature Secondary Structure
MQRCAWADSSPAMQAYHDNEWGRPQHNQQRLFELLCLETYQAGLSWQTVLNKRAAFNEDF
CCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
ADYDVDQVAAMTTADVERLLTDARIIRHRQKLMATINNARVIRDWPAGEDFATWLWAFVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHC
NQPIRQAWASIDEVPATNDLAQQVSKEMKRRGFKFVGPTTIYSYLQATGLINDHVVDCPF
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCC
APENLPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]