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The map label for this gene is pts2CB [H]

Identifier: 28377160

GI number: 28377160

Start: 208913

End: 210751

Strand: Direct

Name: pts2CB [H]

Synonym: lp_0230

Alternate gene names: 28377160

Gene position: 208913-210751 (Clockwise)

Preceding gene: 28377158

Following gene: 28377161

Centisome position: 6.31

GC content: 44.15

Gene sequence:

>1839_bases
ATGAGTGAAAATGTAAATACGAATGCAACCCAAAGCGCTCCTGTTGCGAAAAAGAAACTGGGCGTTAAGGCACGCGTTCA
AAAATTTGGGAGTGCACTAAGTAATATGGTTATGCCGAACATCGGCGCCTTCATTGCTTGGGGCTTAATTACCGCCATCT
TTATGGCTGGTGGTTGGTGGCCTAATGCTGGATTAGCTAAGATGATTCAACCAATGGTCCATTATTTATTGCCATTATTG
ATAGCGTTTACTGGTGGGACTTTGTTTGCCGGTCATCGTGGTGGGGTCGTTGGTGCGATTGCCACTATGGGGGTCATCGT
TGGTTCTTCAATCCCAATGTTTATCGGTGCCATGATTATGGGGCCTTTAGGCGGTTGGTGCATTAAGAAGTGGGATGACG
CCATTTCCGATAAGATTAAGAGCGGGTTTGAAATGTTGGTTAACAACTTCTCCGCTGGGATTATCGGGATGTTACTAGCA
ATCCTGGGTTACTTTGCAATCGGCCCATTGGTTGCTGGTGCAAGTGCTGCTATGGCTGCCGGTGTTGACTGGATCATTAA
GATGAAGCTATTGCCATTAGCCAACGTCTTCATTGAACCAGCCAAGATTTTATTCTTAAACAATGCGATTAACCAAGGTA
TCTTAACACCGCTTGGGATTCAGGCTGCATCTAGTGCGGGTAAGTCAATTCTGTTCTTGCTAGAACCAGATCCAGGTCCA
GGTCTTGGGGTCTTATTAGCCTTTGCCTTGTTTGGTAAAGGGACTGCTAAGGCTTCTGCACCCAGTGCGATTATTATTCA
TTTTCTTGGTGGGATTCATGAAATTTATTTCCCATACGTATTAATGAAGCCAGCATTGTTCCTAGCTGTTATCGCTGGTG
GGGTTACTGGGACAGCCACATTCAGTTTACTAAACGTTGGTTTACGTTCAACACCTTCACCTGGTTCCATTATTTCATTA
CTATTAATGTCACCAAAGAGTATTTCTAACTACATTGGTTTAATTATCGGGGTAACGTTAGCAACCTTAGCATCATTTAT
CGTTGCCGCAATTGTCTTGAAACGTGACAAGTCGACCGGTGATGATGACTTAGCCGCTGCTCAAGGTAAAATGAGCGACA
TGAAGAGTGGCAATGCTGAAGCTGCTAGTGCGACTGACGCTGAACCAGCTGATGTGATTGCTTCATACAAAGATGTTGAC
CATATTATTTTTGCCTGTGATGCCGGAATGGGATCATCCGCAATGGGGGCTTCCTTATTACGGGATAAAGTCAAGAAGGC
TGGCATTAGCATGTCCGTCACCAACACGGCGATTAGCAATTTGAAAGACGAACCTGGTTTATTAGTGATTACGCAAAACG
AATTAGCTGATCGGGCGGCTCAAAAGGCACCACACGCCTTACGGTTAGCCGTTGACAACTTCTTGAGTAGTCCAAAGTAT
GACCAAGTTGTGGTTAACTTAAAGGCTCGTGATCAAGTTAGCGATGCTGCACCTGCATCCGCGGCACCACAAGCAACGAC
GACTGCACCCGACGACCTACCAGCTAACTTAGATTTGAGTGTTGTTGATGAAATTGTCTTTGTTCACCACGATGGTCATT
TAGGAACAGCCACAATGGCAACGTCCTTGATGAAGGATCGGGTCAAGAAAGCCAACAAGCATGTGAGTGTTAAGAACTTA
GGAATTGACGAATTGGCTGATAATAATAGTTTATTAGTTGTTTCAAGTGCAGAAGCAGCTAAAAACTTAAAGACCCGTTA
CACGCAGGTCCAAGTATTAATTACTGACGATCTATTGAGTTCACCTAAATATGATAAGATGGTATCAGAATTGAAATAA

Upstream 100 bases:

>100_bases
GCACAGTTTCCTGTGCCACGGGTCTAACTAGAAGTTCTTGAAACCGCTTACCACGATGGATATGATTAAGTTGTTAAGAA
AAAGGAAGGAGCTTTACCTA

Downstream 100 bases:

>100_bases
AATGATATTATTACCATTATCGTATGAGGTGGCATCATGGTTAAGTTTACACAACGTCAAGATGAGTTGCTACAGCTGCT
ACTAGCGAATCCCGCCGGCC

Product: mannitol PTS, EIICB

Products: NA

Alternate protein names: EIICB-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MSENVNTNATQSAPVAKKKLGVKARVQKFGSALSNMVMPNIGAFIAWGLITAIFMAGGWWPNAGLAKMIQPMVHYLLPLL
IAFTGGTLFAGHRGGVVGAIATMGVIVGSSIPMFIGAMIMGPLGGWCIKKWDDAISDKIKSGFEMLVNNFSAGIIGMLLA
ILGYFAIGPLVAGASAAMAAGVDWIIKMKLLPLANVFIEPAKILFLNNAINQGILTPLGIQAASSAGKSILFLLEPDPGP
GLGVLLAFALFGKGTAKASAPSAIIIHFLGGIHEIYFPYVLMKPALFLAVIAGGVTGTATFSLLNVGLRSTPSPGSIISL
LLMSPKSISNYIGLIIGVTLATLASFIVAAIVLKRDKSTGDDDLAAAQGKMSDMKSGNAEAASATDAEPADVIASYKDVD
HIIFACDAGMGSSAMGASLLRDKVKKAGISMSVTNTAISNLKDEPGLLVITQNELADRAAQKAPHALRLAVDNFLSSPKY
DQVVVNLKARDQVSDAAPASAAPQATTTAPDDLPANLDLSVVDEIVFVHHDGHLGTATMATSLMKDRVKKANKHVSVKNL
GIDELADNNSLLVVSSAEAAKNLKTRYTQVQVLITDDLLSSPKYDKMVSELK

Sequences:

>Translated_612_residues
MSENVNTNATQSAPVAKKKLGVKARVQKFGSALSNMVMPNIGAFIAWGLITAIFMAGGWWPNAGLAKMIQPMVHYLLPLL
IAFTGGTLFAGHRGGVVGAIATMGVIVGSSIPMFIGAMIMGPLGGWCIKKWDDAISDKIKSGFEMLVNNFSAGIIGMLLA
ILGYFAIGPLVAGASAAMAAGVDWIIKMKLLPLANVFIEPAKILFLNNAINQGILTPLGIQAASSAGKSILFLLEPDPGP
GLGVLLAFALFGKGTAKASAPSAIIIHFLGGIHEIYFPYVLMKPALFLAVIAGGVTGTATFSLLNVGLRSTPSPGSIISL
LLMSPKSISNYIGLIIGVTLATLASFIVAAIVLKRDKSTGDDDLAAAQGKMSDMKSGNAEAASATDAEPADVIASYKDVD
HIIFACDAGMGSSAMGASLLRDKVKKAGISMSVTNTAISNLKDEPGLLVITQNELADRAAQKAPHALRLAVDNFLSSPKY
DQVVVNLKARDQVSDAAPASAAPQATTTAPDDLPANLDLSVVDEIVFVHHDGHLGTATMATSLMKDRVKKANKHVSVKNL
GIDELADNNSLLVVSSAEAAKNLKTRYTQVQVLITDDLLSSPKYDKMVSELK
>Mature_611_residues
SENVNTNATQSAPVAKKKLGVKARVQKFGSALSNMVMPNIGAFIAWGLITAIFMAGGWWPNAGLAKMIQPMVHYLLPLLI
AFTGGTLFAGHRGGVVGAIATMGVIVGSSIPMFIGAMIMGPLGGWCIKKWDDAISDKIKSGFEMLVNNFSAGIIGMLLAI
LGYFAIGPLVAGASAAMAAGVDWIIKMKLLPLANVFIEPAKILFLNNAINQGILTPLGIQAASSAGKSILFLLEPDPGPG
LGVLLAFALFGKGTAKASAPSAIIIHFLGGIHEIYFPYVLMKPALFLAVIAGGVTGTATFSLLNVGLRSTPSPGSIISLL
LMSPKSISNYIGLIIGVTLATLASFIVAAIVLKRDKSTGDDDLAAAQGKMSDMKSGNAEAASATDAEPADVIASYKDVDH
IIFACDAGMGSSAMGASLLRDKVKKAGISMSVTNTAISNLKDEPGLLVITQNELADRAAQKAPHALRLAVDNFLSSPKYD
QVVVNLKARDQVSDAAPASAAPQATTTAPDDLPANLDLSVVDEIVFVHHDGHLGTATMATSLMKDRVKKANKHVSVKNLG
IDELADNNSLLVVSSAEAAKNLKTRYTQVQVLITDDLLSSPKYDKMVSELK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=497, Percent_Identity=56.1368209255533, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1789301, Length=470, Percent_Identity=48.7234042553191, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 63846; Mature: 63715

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENVNTNATQSAPVAKKKLGVKARVQKFGSALSNMVMPNIGAFIAWGLITAIFMAGGWW
CCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PNAGLAKMIQPMVHYLLPLLIAFTGGTLFAGHRGGVVGAIATMGVIVGSSIPMFIGAMIM
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
GPLGGWCIKKWDDAISDKIKSGFEMLVNNFSAGIIGMLLAILGYFAIGPLVAGASAAMAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GVDWIIKMKLLPLANVFIEPAKILFLNNAINQGILTPLGIQAASSAGKSILFLLEPDPGP
CHHHHHHHHHHHHHHHHHCCHHEEEEECHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCC
GLGVLLAFALFGKGTAKASAPSAIIIHFLGGIHEIYFPYVLMKPALFLAVIAGGVTGTAT
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FSLLNVGLRSTPSPGSIISLLLMSPKSISNYIGLIIGVTLATLASFIVAAIVLKRDKSTG
HHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDDLAAAQGKMSDMKSGNAEAASATDAEPADVIASYKDVDHIIFACDAGMGSSAMGASLL
CCHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHH
RDKVKKAGISMSVTNTAISNLKDEPGLLVITQNELADRAAQKAPHALRLAVDNFLSSPKY
HHHHHHCCCCEEEHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCC
DQVVVNLKARDQVSDAAPASAAPQATTTAPDDLPANLDLSVVDEIVFVHHDGHLGTATMA
CEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCCCCHHHHH
TSLMKDRVKKANKHVSVKNLGIDELADNNSLLVVSSAEAAKNLKTRYTQVQVLITDDLLS
HHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEECHHHHHHHHHHHEEEEEEEEHHHHC
SPKYDKMVSELK
CCCHHHHHHHCC
>Mature Secondary Structure 
SENVNTNATQSAPVAKKKLGVKARVQKFGSALSNMVMPNIGAFIAWGLITAIFMAGGWW
CCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PNAGLAKMIQPMVHYLLPLLIAFTGGTLFAGHRGGVVGAIATMGVIVGSSIPMFIGAMIM
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
GPLGGWCIKKWDDAISDKIKSGFEMLVNNFSAGIIGMLLAILGYFAIGPLVAGASAAMAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GVDWIIKMKLLPLANVFIEPAKILFLNNAINQGILTPLGIQAASSAGKSILFLLEPDPGP
CHHHHHHHHHHHHHHHHHCCHHEEEEECHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCC
GLGVLLAFALFGKGTAKASAPSAIIIHFLGGIHEIYFPYVLMKPALFLAVIAGGVTGTAT
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FSLLNVGLRSTPSPGSIISLLLMSPKSISNYIGLIIGVTLATLASFIVAAIVLKRDKSTG
HHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDDLAAAQGKMSDMKSGNAEAASATDAEPADVIASYKDVDHIIFACDAGMGSSAMGASLL
CCHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHH
RDKVKKAGISMSVTNTAISNLKDEPGLLVITQNELADRAAQKAPHALRLAVDNFLSSPKY
HHHHHHCCCCEEEHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCC
DQVVVNLKARDQVSDAAPASAAPQATTTAPDDLPANLDLSVVDEIVFVHHDGHLGTATMA
CEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEECCCCCHHHHH
TSLMKDRVKKANKHVSVKNLGIDELADNNSLLVVSSAEAAKNLKTRYTQVQVLITDDLLS
HHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEEEECHHHHHHHHHHHEEEEEEEEHHHHC
SPKYDKMVSELK
CCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]