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The map label for this gene is azoR1

Identifier: 28377037

GI number: 28377037

Start: 72330

End: 72977

Strand: Direct

Name: azoR1

Synonym: lp_0075

Alternate gene names: 28377037

Gene position: 72330-72977 (Clockwise)

Preceding gene: 28377035

Following gene: 28377038

Centisome position: 2.19

GC content: 42.75

Gene sequence:

>648_bases
ATGACTAAGACTTTGATTGTTAATGCACATCCCGATTTTCGTAATGCGGCGCACTACTCAGTTCAACTAGAACAAGCTTT
TTTGCAACTTTTTCAGACCCGCTTTCCTAATGATACTGTGGATGTGCTCAATTTGTATGATACGGTGATTCCCCAAGCAA
CGGTACCTGAATTGCTGGGAATCTGGGAAAAGCAAGCGCAACATGTAAATTTATCGATTGAGGAACAACGCCTGTTTGCA
ATCAATCAGCAACTCCTACAACAGTTTAAAGCTCATCATCGGATTGTGATTGCGATGCCGTTGCATAATTTCAACGTGCC
GGCACGGTTAAAGGACTATATCGATAATATCTTAGTTGCCAGGGAGACTTTTCGTTATACCGAAAATGGATCAGTGGGTT
TGATGACCGATAATTATCGTGTCATGTTACTGCAGGCCAGTGGGTCAATCTATACTCGTAATGACCGTTATACACCCATG
GAATTTTCTCGGCTGTATCTTGACAAGATGTTTACTGAAATTATGGGGTTTGACCGGTTTGAGATTGTCCGTGCACAAGG
ACTGCAAACGAACGGGGTGGCGGTATCACAGGCATTAAAGCAAGCCAAAATGGACCTGAAGGCAGCTTTTGAGCGTTTTT
ATGATTAA

Upstream 100 bases:

>100_bases
ATGATTTTAATGGTGACATGGATTGGCACCTTTCGACTGTAGTATATAACTGTTGTCAAAAACAACCAATAAATAGATTA
TTAATTAAGGAGATATTTAA

Downstream 100 bases:

>100_bases
GTCTGAAAATGATTTTAAGGTATCGGTAGTTGTAGGCTACGGATACCTTTTTTGCGATACTGCACGGGAAATGGGTCACC
ACTACTTTAAAGTGATCGCA

Product: acyl carrier protein phosphodiesterase (putative)

Products: 4'- Phosphopantetheine; Apoprotein. [C]

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTKTLIVNAHPDFRNAAHYSVQLEQAFLQLFQTRFPNDTVDVLNLYDTVIPQATVPELLGIWEKQAQHVNLSIEEQRLFA
INQQLLQQFKAHHRIVIAMPLHNFNVPARLKDYIDNILVARETFRYTENGSVGLMTDNYRVMLLQASGSIYTRNDRYTPM
EFSRLYLDKMFTEIMGFDRFEIVRAQGLQTNGVAVSQALKQAKMDLKAAFERFYD

Sequences:

>Translated_215_residues
MTKTLIVNAHPDFRNAAHYSVQLEQAFLQLFQTRFPNDTVDVLNLYDTVIPQATVPELLGIWEKQAQHVNLSIEEQRLFA
INQQLLQQFKAHHRIVIAMPLHNFNVPARLKDYIDNILVARETFRYTENGSVGLMTDNYRVMLLQASGSIYTRNDRYTPM
EFSRLYLDKMFTEIMGFDRFEIVRAQGLQTNGVAVSQALKQAKMDLKAAFERFYD
>Mature_214_residues
TKTLIVNAHPDFRNAAHYSVQLEQAFLQLFQTRFPNDTVDVLNLYDTVIPQATVPELLGIWEKQAQHVNLSIEEQRLFAI
NQQLLQQFKAHHRIVIAMPLHNFNVPARLKDYIDNILVARETFRYTENGSVGLMTDNYRVMLLQASGSIYTRNDRYTPME
FSRLYLDKMFTEIMGFDRFEIVRAQGLQTNGVAVSQALKQAKMDLKAAFERFYD

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR1_LACPL (Q890E7)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783929.1
- ProteinModelPortal:   Q890E7
- GeneID:   1061266
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0075
- NMPDR:   fig|220668.1.peg.63
- HOGENOM:   HBG565834
- OMA:   PLHNFNV
- ProtClustDB:   CLSK904463
- BioCyc:   LPLA220668:LP_0075-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14 [C]

Molecular weight: Translated: 25011; Mature: 24880

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLIVNAHPDFRNAAHYSVQLEQAFLQLFQTRFPNDTVDVLNLYDTVIPQATVPELLG
CCCEEEEECCCCCCCCHHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
IWEKQAQHVNLSIEEQRLFAINQQLLQQFKAHHRIVIAMPLHNFNVPARLKDYIDNILVA
HHHHHHHEECCEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
RETFRYTENGSVGLMTDNYRVMLLQASGSIYTRNDRYTPMEFSRLYLDKMFTEIMGFDRF
HHHHEECCCCCEEEEECCEEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHH
EIVRAQGLQTNGVAVSQALKQAKMDLKAAFERFYD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKTLIVNAHPDFRNAAHYSVQLEQAFLQLFQTRFPNDTVDVLNLYDTVIPQATVPELLG
CCEEEEECCCCCCCCHHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHH
IWEKQAQHVNLSIEEQRLFAINQQLLQQFKAHHRIVIAMPLHNFNVPARLKDYIDNILVA
HHHHHHHEECCEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
RETFRYTENGSVGLMTDNYRVMLLQASGSIYTRNDRYTPMEFSRLYLDKMFTEIMGFDRF
HHHHEECCCCCEEEEECCEEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHH
EIVRAQGLQTNGVAVSQALKQAKMDLKAAFERFYD
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [Acyl-Carrier Protein]; H2O [C]

Specific reaction: [Acyl-Carrier Protein] + H2O = 4'- Phosphopantetheine + Apoprotein. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566