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The map label for this gene is glgB

Identifier: 28376992

GI number: 28376992

Start: 21099

End: 23003

Strand: Direct

Name: glgB

Synonym: lp_0020

Alternate gene names: 28376992

Gene position: 21099-23003 (Clockwise)

Preceding gene: 28376990

Following gene: 28376993

Centisome position: 0.64

GC content: 47.72

Gene sequence:

>1905_bases
TTGGTTAAACAAACTACGGAAATGAATGATGCAAGTTACTTATTTAATACCGGCGCAGGATTTAATAGTCAGGATTACTT
AGGCTGTCATTTGGCCGCATCTGGTCGGGCGGTATTTCGCGTCTGGGCGCCGCATGCTAAGGCGGTCGGGGTTGTTGGCG
ATTTTAATGACTGGCAACCAAGCGCACTAAAGTTATTAGGGGCGACTGGGATTTGGCAAGGACAAGTTCCGAACGTTCAT
GCGGGTCAGTTATACAAGTTTCAGATCACAACACCAACTGGCCAAGTGCAACTAAAGATTGACCCGTTTGCACAACAGTT
CGAACGAAAACCGGGGGATGCGGCCGTAGTTGTTGAGCCGATGACGATGCGTTGGCACGATAGTTTGTGGCTAGCTCGCC
GGAAGAAGTCGCGCGCTGCCAATCGGCCAATTAACATTTATGAGGTGCACTTAGGTTCGTGGCGCCGTCATCTTGATGGT
AGCTACTACACTTATGCGGAATTAGCCGCTGAACTGATTCCCTATGTAAAACAACGGGGCTATACCCACATTGAATTAAT
GCCCGTGATGGAACATTTATTAGATGCGTCTTGGGGTTATCAGCAATTAGGGTATTTTGCGCCAACCAGTCGTTTTGGCA
AGCGTGACAGTTTTCTCAGCTTTGTTGACCAGTGTCACCAAGCGAATATCGGGGTATTCGTTGATTGGGTACCGGGTCAC
TTTATTCGCAATTATGATGCGTTGTACCAGTATGATGGTACGCCGACATTTGAATACGCTGATCCTGAGCGCGCGAATAA
CCGTCGTTGGGGGGCGTGGAATTTTGATCTCGGTAAAACACAGGTTCAGAGTTTTCTGATTTCAAGCGCCGAATATTGGC
TCGACCAGTGTCATCTAGATGGCTTGCGAGTGGATGCTGTCTCTAACATGTTGTATTGCGACTATGATGAAGGGCGCGAA
GGGCAGGTTAATCAGTATGGGGATAATCGTAATTTAGAAGGCATCGCCTTTTTACAAAAGTTGAACACCACGGTCTTAAC
CCACCATCCCGATATCTTGATGATTGCGGAAGAGTCGTCCGCCTATCCGCAGGTTACGGGTTCGGTGGCCAATGGCGGAT
TGGGATTTAACTATAAGTGGAACATGGGGTGGATGCACGATACCCTGGATTTCTTTATGATGGATCCGTTATATCGGCAC
GATCACTTTAATCTACTGACATTTGCATTCGTGTACATGTTCGATGAACGGTTTATTCTACCGTTTTCACATGATGAAGT
CGTGCACGGCAAGAAATCATTAATGCATAAAATGTCGGGGGACCGCTATAATCAGTTTGCAAATTTGCGGACGATGCTAA
CTTATATGGCTGCCTTTCCAGGAAAACAACTGAATTTTATGGGTTCAGAGTGGGGGCAGTTTCTAGAGTGGCGGGACTGG
AGTGCGTTGGAATGGGTCGACTTAAAGGATGATCTCAACCATCGGATGCAACAGTTCACAACGCAACTGAACGCGGTCTA
TCGGCACAATCGGCCGTTGTGGGAACAAGACCATGATCCTGCGGGGATTATTTATACACATACCGATGATCCTGACAGTT
CGACCATGGGGTTTATCCGGCAGGCCAAACGAAAATACAGTTTTGTGGTCGCTGCTTTCAATTTTGTTCCTGTTGAACGT
CAAGATTACCGGATTGGCGTACCGTATCGTGGCCGTTATGAGTTGTTACTCAATACTGAAGCGCAGGCTTTTGGCGGCAC
GTGGACAAAGCTAGAAACAACGTTTACGGCGGTCGATAAGCCGTATCGGGGGCAACCAGCTAGTTTTACCGTGACCTTGC
CGGCGATGGGTGCATTGTTGATTCGACCAGTTAAAGTGATAGGGGGAGTTAAGCATGCAAGATGA

Upstream 100 bases:

>100_bases
TAAGGCGACCTTATCAAGGATAGGCAAACACCCTGTCACCTTCTTATAATAAGATTAATTTTATAAACTGCTCAGTTAAT
CACATAGGGGGTGTGATGCT

Downstream 100 bases:

>100_bases
AATGTTAGGGATTATTTTAGCGGGCGGTCAGGGCACCCGGCTTGGTAAGTTGACAAAGGATACGGCTAAGCCCGCCGTGC
CATTCGGCGGTCGTTACCGA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MVKQTTEMNDASYLFNTGAGFNSQDYLGCHLAASGRAVFRVWAPHAKAVGVVGDFNDWQPSALKLLGATGIWQGQVPNVH
AGQLYKFQITTPTGQVQLKIDPFAQQFERKPGDAAVVVEPMTMRWHDSLWLARRKKSRAANRPINIYEVHLGSWRRHLDG
SYYTYAELAAELIPYVKQRGYTHIELMPVMEHLLDASWGYQQLGYFAPTSRFGKRDSFLSFVDQCHQANIGVFVDWVPGH
FIRNYDALYQYDGTPTFEYADPERANNRRWGAWNFDLGKTQVQSFLISSAEYWLDQCHLDGLRVDAVSNMLYCDYDEGRE
GQVNQYGDNRNLEGIAFLQKLNTTVLTHHPDILMIAEESSAYPQVTGSVANGGLGFNYKWNMGWMHDTLDFFMMDPLYRH
DHFNLLTFAFVYMFDERFILPFSHDEVVHGKKSLMHKMSGDRYNQFANLRTMLTYMAAFPGKQLNFMGSEWGQFLEWRDW
SALEWVDLKDDLNHRMQQFTTQLNAVYRHNRPLWEQDHDPAGIIYTHTDDPDSSTMGFIRQAKRKYSFVVAAFNFVPVER
QDYRIGVPYRGRYELLLNTEAQAFGGTWTKLETTFTAVDKPYRGQPASFTVTLPAMGALLIRPVKVIGGVKHAR

Sequences:

>Translated_634_residues
MVKQTTEMNDASYLFNTGAGFNSQDYLGCHLAASGRAVFRVWAPHAKAVGVVGDFNDWQPSALKLLGATGIWQGQVPNVH
AGQLYKFQITTPTGQVQLKIDPFAQQFERKPGDAAVVVEPMTMRWHDSLWLARRKKSRAANRPINIYEVHLGSWRRHLDG
SYYTYAELAAELIPYVKQRGYTHIELMPVMEHLLDASWGYQQLGYFAPTSRFGKRDSFLSFVDQCHQANIGVFVDWVPGH
FIRNYDALYQYDGTPTFEYADPERANNRRWGAWNFDLGKTQVQSFLISSAEYWLDQCHLDGLRVDAVSNMLYCDYDEGRE
GQVNQYGDNRNLEGIAFLQKLNTTVLTHHPDILMIAEESSAYPQVTGSVANGGLGFNYKWNMGWMHDTLDFFMMDPLYRH
DHFNLLTFAFVYMFDERFILPFSHDEVVHGKKSLMHKMSGDRYNQFANLRTMLTYMAAFPGKQLNFMGSEWGQFLEWRDW
SALEWVDLKDDLNHRMQQFTTQLNAVYRHNRPLWEQDHDPAGIIYTHTDDPDSSTMGFIRQAKRKYSFVVAAFNFVPVER
QDYRIGVPYRGRYELLLNTEAQAFGGTWTKLETTFTAVDKPYRGQPASFTVTLPAMGALLIRPVKVIGGVKHAR
>Mature_634_residues
MVKQTTEMNDASYLFNTGAGFNSQDYLGCHLAASGRAVFRVWAPHAKAVGVVGDFNDWQPSALKLLGATGIWQGQVPNVH
AGQLYKFQITTPTGQVQLKIDPFAQQFERKPGDAAVVVEPMTMRWHDSLWLARRKKSRAANRPINIYEVHLGSWRRHLDG
SYYTYAELAAELIPYVKQRGYTHIELMPVMEHLLDASWGYQQLGYFAPTSRFGKRDSFLSFVDQCHQANIGVFVDWVPGH
FIRNYDALYQYDGTPTFEYADPERANNRRWGAWNFDLGKTQVQSFLISSAEYWLDQCHLDGLRVDAVSNMLYCDYDEGRE
GQVNQYGDNRNLEGIAFLQKLNTTVLTHHPDILMIAEESSAYPQVTGSVANGGLGFNYKWNMGWMHDTLDFFMMDPLYRH
DHFNLLTFAFVYMFDERFILPFSHDEVVHGKKSLMHKMSGDRYNQFANLRTMLTYMAAFPGKQLNFMGSEWGQFLEWRDW
SALEWVDLKDDLNHRMQQFTTQLNAVYRHNRPLWEQDHDPAGIIYTHTDDPDSSTMGFIRQAKRKYSFVVAAFNFVPVER
QDYRIGVPYRGRYELLLNTEAQAFGGTWTKLETTFTAVDKPYRGQPASFTVTLPAMGALLIRPVKVIGGVKHAR

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=653, Percent_Identity=27.2588055130168, Blast_Score=187, Evalue=3e-47,
Organism=Escherichia coli, GI1789839, Length=626, Percent_Identity=40.4153354632588, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17554896, Length=636, Percent_Identity=27.0440251572327, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI32564391, Length=576, Percent_Identity=27.0833333333333, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=677, Percent_Identity=24.9630723781388, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28573410, Length=643, Percent_Identity=26.2830482115086, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_LACPL (Q890J1)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783884.1
- ProteinModelPortal:   Q890J1
- SMR:   Q890J1
- GeneID:   1061293
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0020
- NMPDR:   fig|220668.1.peg.18
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK12313
- BioCyc:   LPLA220668:LP_0020-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 72981; Mature: 72981

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: ACT_SITE 200-200 ACT_SITE 235-235 ACT_SITE 240-240 ACT_SITE 303-303 ACT_SITE 305-305 ACT_SITE 357-357 ACT_SITE 424-424 ACT_SITE 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQTTEMNDASYLFNTGAGFNSQDYLGCHLAASGRAVFRVWAPHAKAVGVVGDFNDWQP
CCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCH
SALKLLGATGIWQGQVPNVHAGQLYKFQITTPTGQVQLKIDPFAQQFERKPGDAAVVVEP
HHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECHHHHHHHCCCCCEEEEEEC
MTMRWHDSLWLARRKKSRAANRPINIYEVHLGSWRRHLDGSYYTYAELAAELIPYVKQRG
EEEEECHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEHHHHHHHHHHHHHHHCC
YTHIELMPVMEHLLDASWGYQQLGYFAPTSRFGKRDSFLSFVDQCHQANIGVFVDWVPGH
CCEEEHHHHHHHHHCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEECCCCH
FIRNYDALYQYDGTPTFEYADPERANNRRWGAWNFDLGKTQVQSFLISSAEYWLDQCHLD
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVSNMLYCDYDEGREGQVNQYGDNRNLEGIAFLQKLNTTVLTHHPDILMIAEESS
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCEEEEECCCC
AYPQVTGSVANGGLGFNYKWNMGWMHDTLDFFMMDPLYRHDHFNLLTFAFVYMFDERFIL
CCCEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCEEE
PFSHDEVVHGKKSLMHKMSGDRYNQFANLRTMLTYMAAFPGKQLNFMGSEWGQFLEWRDW
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCCCCC
SALEWVDLKDDLNHRMQQFTTQLNAVYRHNRPLWEQDHDPAGIIYTHTDDPDSSTMGFIR
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
QAKRKYSFVVAAFNFVPVERQDYRIGVPYRGRYELLLNTEAQAFGGTWTKLETTFTAVDK
HHHHHHEEEEEEEEEEECCCCCCEECCCCCCCEEEEEECCCHHCCCCEEEEEEEEEECCC
PYRGQPASFTVTLPAMGALLIRPVKVIGGVKHAR
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVKQTTEMNDASYLFNTGAGFNSQDYLGCHLAASGRAVFRVWAPHAKAVGVVGDFNDWQP
CCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCH
SALKLLGATGIWQGQVPNVHAGQLYKFQITTPTGQVQLKIDPFAQQFERKPGDAAVVVEP
HHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECHHHHHHHCCCCCEEEEEEC
MTMRWHDSLWLARRKKSRAANRPINIYEVHLGSWRRHLDGSYYTYAELAAELIPYVKQRG
EEEEECHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEHHHHHHHHHHHHHHHCC
YTHIELMPVMEHLLDASWGYQQLGYFAPTSRFGKRDSFLSFVDQCHQANIGVFVDWVPGH
CCEEEHHHHHHHHHCCCCCHHHHCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEECCCCH
FIRNYDALYQYDGTPTFEYADPERANNRRWGAWNFDLGKTQVQSFLISSAEYWLDQCHLD
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVSNMLYCDYDEGREGQVNQYGDNRNLEGIAFLQKLNTTVLTHHPDILMIAEESS
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCEEEEECCCC
AYPQVTGSVANGGLGFNYKWNMGWMHDTLDFFMMDPLYRHDHFNLLTFAFVYMFDERFIL
CCCEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCEEE
PFSHDEVVHGKKSLMHKMSGDRYNQFANLRTMLTYMAAFPGKQLNFMGSEWGQFLEWRDW
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCCCCC
SALEWVDLKDDLNHRMQQFTTQLNAVYRHNRPLWEQDHDPAGIIYTHTDDPDSSTMGFIR
CCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
QAKRKYSFVVAAFNFVPVERQDYRIGVPYRGRYELLLNTEAQAFGGTWTKLETTFTAVDK
HHHHHHEEEEEEEEEEECCCCCCEECCCCCCCEEEEEECCCHHCCCCEEEEEEEEEECCC
PYRGQPASFTVTLPAMGALLIRPVKVIGGVKHAR
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566