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The map label for this gene is nasD [H]

Identifier: 28212100

GI number: 28212100

Start: 2677155

End: 2678456

Strand: Direct

Name: nasD [H]

Synonym: CTC02521

Alternate gene names: 28212100

Gene position: 2677155-2678456 (Clockwise)

Preceding gene: 28212092

Following gene: 28212113

Centisome position: 95.64

GC content: 29.8

Gene sequence:

>1302_bases
ATGTATAAAAAATGGAAATGCTTAGTTTGTGGTGTTATATTTGATGGTGATGTACCACCTAATCCTTGTCCTGTATGTGG
AGCTTCTTCTGATAATTTTGAAGAAGTACTAGTAGCTAGAAATGATTTTGCAGATGATTCAAAATTAAATTTTGTAATTA
TAGGAAATGGAGCTGCAGGCTTCTATGCTGCAGATTCTGTAAGAAATAGAAACAAAAACTGTAATATTACTATGATATCT
AAAGAGAAGACATATAGTTATTACAGACCTTTACTTTCAGAATATTTAAATAAAGAGAAGCTACCATCTAATTTTTATTT
ATCAAAGGAAAATTGGTTTGAAGAAAAAAATATAAATCTTATTTTAAATACTGCTGTTAATAATATTAATCCTGTAGATA
AAAAAGTTCTGTTATCTAATGGTAAAACATTGAATTACGACAAATTAATATTGGCTAATGGTGGATACAATTTTATTCCA
CCCATTGAAGGTAAAGATAAAAAAGGAGTATTTACTTTAAGGGGTTTAGAAGATTCTGAAAACATTAAAGATTATGCTAA
AAAAATTAAACATGCAACAGTTATTGGTGGCGGATTACTAGGACTGGAAGCTGCTTGGGAATTAAGAAACATAGGACTAG
ATGTTTCGGTTATAGAGTTCTTTGATAGATTACTTCCACGTCAGCTAGATGAAAAAGCTGGAAAACTGTTTCAGGACTCT
ATAAAACAATCCAATGTAGACATAATATTAGGTGATTCTCTTGTAGAAATTTTAGGAGATAATTCAGTAGAATCTATTAA
ACTAAAAAGTGGTAAAGAAATAAAAACTGATATGGTTTTATTTTCTATTGGTATAAGATCTAACAAAGAGTTAGCAGAAA
ACTGCGGATTGAAAACCGATAAGGCTGTCATAGTAAATGAAAAAATGGAAACAAATATTGATGATATTTATGCTTGTGGC
GATGTAGCTGAGTTCAATGGAAAGAACTATGGTAACTGGCCTGCCGCAGTGCAGATGGGTAAAGTCGCTGGTGCTAATGC
CTCTGGAGATAATTTGAACTTTAAAGATTTTGTTTCAGCAATTATCTTTAGAGCTATGAACACTAAAATGTTTACTTGTG
GTAATTGTGATGATAATATGGTCAATGTAGCTTATGAAGATCCTAAAAATGGAACATACAAAAAATTATTCTTTGAAAAA
GATATATTGGTAGGTGCTACTTTATTAGGTGATATATCTTCATCAGGTAAATTAGTTGAACAAATTAAAAATAAAACTTC
TATAAATGATATAATAAAATAG

Upstream 100 bases:

>100_bases
TAAAAATAAACAATGAATAACCTTTAAAAAGTTGATATACATATATTATTACTATATACTTATCATTAACTAATAAATTT
TTTATATTTGAGGTGTTGCT

Downstream 100 bases:

>100_bases
AATGCATTAAAATAAGGGCTATTCTCTAGCCCTTATTTTAATACTTTCATGAATAGATATGAATGCATTTAACTCTCCAC
CTAGAATTATAATAATAGAA

Product: NADH dehydrogenase, nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MYKKWKCLVCGVIFDGDVPPNPCPVCGASSDNFEEVLVARNDFADDSKLNFVIIGNGAAGFYAADSVRNRNKNCNITMIS
KEKTYSYYRPLLSEYLNKEKLPSNFYLSKENWFEEKNINLILNTAVNNINPVDKKVLLSNGKTLNYDKLILANGGYNFIP
PIEGKDKKGVFTLRGLEDSENIKDYAKKIKHATVIGGGLLGLEAAWELRNIGLDVSVIEFFDRLLPRQLDEKAGKLFQDS
IKQSNVDIILGDSLVEILGDNSVESIKLKSGKEIKTDMVLFSIGIRSNKELAENCGLKTDKAVIVNEKMETNIDDIYACG
DVAEFNGKNYGNWPAAVQMGKVAGANASGDNLNFKDFVSAIIFRAMNTKMFTCGNCDDNMVNVAYEDPKNGTYKKLFFEK
DILVGATLLGDISSSGKLVEQIKNKTSINDIIK

Sequences:

>Translated_433_residues
MYKKWKCLVCGVIFDGDVPPNPCPVCGASSDNFEEVLVARNDFADDSKLNFVIIGNGAAGFYAADSVRNRNKNCNITMIS
KEKTYSYYRPLLSEYLNKEKLPSNFYLSKENWFEEKNINLILNTAVNNINPVDKKVLLSNGKTLNYDKLILANGGYNFIP
PIEGKDKKGVFTLRGLEDSENIKDYAKKIKHATVIGGGLLGLEAAWELRNIGLDVSVIEFFDRLLPRQLDEKAGKLFQDS
IKQSNVDIILGDSLVEILGDNSVESIKLKSGKEIKTDMVLFSIGIRSNKELAENCGLKTDKAVIVNEKMETNIDDIYACG
DVAEFNGKNYGNWPAAVQMGKVAGANASGDNLNFKDFVSAIIFRAMNTKMFTCGNCDDNMVNVAYEDPKNGTYKKLFFEK
DILVGATLLGDISSSGKLVEQIKNKTSINDIIK
>Mature_433_residues
MYKKWKCLVCGVIFDGDVPPNPCPVCGASSDNFEEVLVARNDFADDSKLNFVIIGNGAAGFYAADSVRNRNKNCNITMIS
KEKTYSYYRPLLSEYLNKEKLPSNFYLSKENWFEEKNINLILNTAVNNINPVDKKVLLSNGKTLNYDKLILANGGYNFIP
PIEGKDKKGVFTLRGLEDSENIKDYAKKIKHATVIGGGLLGLEAAWELRNIGLDVSVIEFFDRLLPRQLDEKAGKLFQDS
IKQSNVDIILGDSLVEILGDNSVESIKLKSGKEIKTDMVLFSIGIRSNKELAENCGLKTDKAVIVNEKMETNIDDIYACG
DVAEFNGKNYGNWPAAVQMGKVAGANASGDNLNFKDFVSAIIFRAMNTKMFTCGNCDDNMVNVAYEDPKNGTYKKLFFEK
DILVGATLLGDISSSGKLVEQIKNKTSINDIIK

Specific function: Required for nitrite assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=311, Percent_Identity=28.2958199356913, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI21389617, Length=311, Percent_Identity=28.2958199356913, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI226437568, Length=311, Percent_Identity=28.2958199356913, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4757732, Length=348, Percent_Identity=27.2988505747126, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22202629, Length=348, Percent_Identity=27.2988505747126, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789765, Length=395, Percent_Identity=30.6329113924051, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788892, Length=274, Percent_Identity=27.007299270073, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=26.5232974910394, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87082354, Length=282, Percent_Identity=27.6595744680851, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=386, Percent_Identity=27.979274611399, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564386, Length=429, Percent_Identity=25.8741258741259, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639257, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281359715, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281359713, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24639250, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18543267, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24639252, Length=362, Percent_Identity=24.585635359116, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585130, Length=366, Percent_Identity=23.4972677595628, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581020, Length=346, Percent_Identity=21.6763005780347, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28573993, Length=346, Percent_Identity=21.6763005780347, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 47975; Mature: 47975

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKKWKCLVCGVIFDGDVPPNPCPVCGASSDNFEEVLVARNDFADDSKLNFVIIGNGAAG
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECCCCC
FYAADSVRNRNKNCNITMISKEKTYSYYRPLLSEYLNKEKLPSNFYLSKENWFEEKNINL
CEEHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCEE
ILNTAVNNINPVDKKVLLSNGKTLNYDKLILANGGYNFIPPIEGKDKKGVFTLRGLEDSE
EEEECCCCCCCCCCEEEECCCCEECCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCH
NIKDYAKKIKHATVIGGGLLGLEAAWELRNIGLDVSVIEFFDRLLPRQLDEKAGKLFQDS
HHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQSNVDIILGDSLVEILGDNSVESIKLKSGKEIKTDMVLFSIGIRSNKELAENCGLKTD
HHHCCCCEEECCHHHHHHCCCCCEEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCCC
KAVIVNEKMETNIDDIYACGDVAEFNGKNYGNWPAAVQMGKVAGANASGDNLNFKDFVSA
CEEEECCHHHCCCHHEEECCCHHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHH
IIFRAMNTKMFTCGNCDDNMVNVAYEDPKNGTYKKLFFEKDILVGATLLGDISSSGKLVE
HHHHHHCCCEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHH
QIKNKTSINDIIK
HHHCCCCHHHHCC
>Mature Secondary Structure
MYKKWKCLVCGVIFDGDVPPNPCPVCGASSDNFEEVLVARNDFADDSKLNFVIIGNGAAG
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEEECCCCC
FYAADSVRNRNKNCNITMISKEKTYSYYRPLLSEYLNKEKLPSNFYLSKENWFEEKNINL
CEEHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCHHCCCCCCCEE
ILNTAVNNINPVDKKVLLSNGKTLNYDKLILANGGYNFIPPIEGKDKKGVFTLRGLEDSE
EEEECCCCCCCCCCEEEECCCCEECCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCH
NIKDYAKKIKHATVIGGGLLGLEAAWELRNIGLDVSVIEFFDRLLPRQLDEKAGKLFQDS
HHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQSNVDIILGDSLVEILGDNSVESIKLKSGKEIKTDMVLFSIGIRSNKELAENCGLKTD
HHHCCCCEEECCHHHHHHCCCCCEEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCCC
KAVIVNEKMETNIDDIYACGDVAEFNGKNYGNWPAAVQMGKVAGANASGDNLNFKDFVSA
CEEEECCHHHCCCHHEEECCCHHHCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHH
IIFRAMNTKMFTCGNCDDNMVNVAYEDPKNGTYKKLFFEKDILVGATLLGDISSSGKLVE
HHHHHHCCCEEEECCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHH
QIKNKTSINDIIK
HHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]