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The map label for this gene is speE

Identifier: 28211850

GI number: 28211850

Start: 2368798

End: 2369643

Strand: Direct

Name: speE

Synonym: CTC02249

Alternate gene names: 28211850

Gene position: 2368798-2369643 (Clockwise)

Preceding gene: 28211843

Following gene: 28211852

Centisome position: 84.62

GC content: 33.45

Gene sequence:

>846_bases
TTGGATTTATGGCTAAAAGAAGGTCAAATTTCAGATGCTGTTATGACCTATAAAATAAAAGAAACCCTTGTTAGGAAAAA
GACTGAATATCAAGACTTGGCTATAGTAGATACTTATGCCCTTGGAAGAATGCTTGTTTTAGATGGCATAGTTCAAACTT
CTATTAAGGATGAATATGTATACCATGAAATGATAACACATATACCTCTTTACACTCACCCAAATCCTAAAAAAGTTTTA
GTTGTTGGTGGTGGAGACGGTGGTACTATAAGAGAAATACTAAAGCACCCATCTGTAGAAAAAGCTGTGCTTTGTGAAAT
AGATGAAGAAGTAGTTAAAGAATGCAAAAAACATCTTCCTGAAATAAGCAATAATGCTTTAGAAGATAGCCGTTGTGAAG
TTTTCATAGGAGATGGAATAAAGTATGTAAATGAACATAAAAATGAATTTGATGTAGTAATAATAGACTCTACTGACCCC
TTCGGAGCAGCTGAAGGACTTTTTGGTGGAAGCTTTTATGGTAAAATATCAGAATGTCTAACAGAAGATGGTATATTTGT
AGCTCAAACAGAAACTCCTTTCTATTTACCTGAAATAGTAAAAAGAGTTTATGATGATTCTAAAGCAGTATTTCCAGTTA
CAAAACTGTTTATGGCTGCTATTCCTACTTATCCATCAGGGTATTGGAGCTTCACTATTGGATCTAAGAAACACGATCCT
GAAAAGGCACCAGTTCCAGAAGTTTTACCTTTTGAAACCAAATATTACACTCCAAGACTACATTCAGCATCATTTGTTTT
ACCTAAATTTGTAGAAAACCTTATATTCGCTAATATTAGCGAATAA

Upstream 100 bases:

>100_bases
ATAGATAATTATAGATATTTTTTCAATATTGCATACAATGGTAAAGCCTGTTATAATAGTCATATTAAAAATATAATAAC
TTGCTAGGAGGCTGATTTTA

Downstream 100 bases:

>100_bases
TTAATAATGAACAATTAATGAAGCTCTTCTTCCTTACGTCGGAAGAGCTTTAATTTTATTTGGTTTATTAAGTATATTTG
TCTCATATGAAGCTAGTTAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDLWLKEGQISDAVMTYKIKETLVRKKTEYQDLAIVDTYALGRMLVLDGIVQTSIKDEYVYHEMITHIPLYTHPNPKKVL
VVGGGDGGTIREILKHPSVEKAVLCEIDEEVVKECKKHLPEISNNALEDSRCEVFIGDGIKYVNEHKNEFDVVIIDSTDP
FGAAEGLFGGSFYGKISECLTEDGIFVAQTETPFYLPEIVKRVYDDSKAVFPVTKLFMAAIPTYPSGYWSFTIGSKKHDP
EKAPVPEVLPFETKYYTPRLHSASFVLPKFVENLIFANISE

Sequences:

>Translated_281_residues
MDLWLKEGQISDAVMTYKIKETLVRKKTEYQDLAIVDTYALGRMLVLDGIVQTSIKDEYVYHEMITHIPLYTHPNPKKVL
VVGGGDGGTIREILKHPSVEKAVLCEIDEEVVKECKKHLPEISNNALEDSRCEVFIGDGIKYVNEHKNEFDVVIIDSTDP
FGAAEGLFGGSFYGKISECLTEDGIFVAQTETPFYLPEIVKRVYDDSKAVFPVTKLFMAAIPTYPSGYWSFTIGSKKHDP
EKAPVPEVLPFETKYYTPRLHSASFVLPKFVENLIFANISE
>Mature_281_residues
MDLWLKEGQISDAVMTYKIKETLVRKKTEYQDLAIVDTYALGRMLVLDGIVQTSIKDEYVYHEMITHIPLYTHPNPKKVL
VVGGGDGGTIREILKHPSVEKAVLCEIDEEVVKECKKHLPEISNNALEDSRCEVFIGDGIKYVNEHKNEFDVVIIDSTDP
FGAAEGLFGGSFYGKISECLTEDGIFVAQTETPFYLPEIVKRVYDDSKAVFPVTKLFMAAIPTYPSGYWSFTIGSKKHDP
EKAPVPEVLPFETKYYTPRLHSASFVLPKFVENLIFANISE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=268, Percent_Identity=38.4328358208955, Blast_Score=217, Evalue=9e-57,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=36.8421052631579, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI25154106, Length=275, Percent_Identity=40.7272727272727, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI212645616, Length=229, Percent_Identity=42.7947598253275, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6325326, Length=281, Percent_Identity=40.5693950177936, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6323175, Length=290, Percent_Identity=36.551724137931, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24645443, Length=279, Percent_Identity=39.0681003584229, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=38.2488479262673, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21358269, Length=168, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24663238, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_CLOTE (Q891W4)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_782794.1
- ProteinModelPortal:   Q891W4
- SMR:   Q891W4
- GeneID:   1060394
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC02249
- NMPDR:   fig|212717.1.peg.2016
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- PhylomeDB:   Q891W4
- ProtClustDB:   PRK00811
- BioCyc:   CTET212717:CTC_02249-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31681; Mature: 31681

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 156-156 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLWLKEGQISDAVMTYKIKETLVRKKTEYQDLAIVDTYALGRMLVLDGIVQTSIKDEYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
YHEMITHIPLYTHPNPKKVLVVGGGDGGTIREILKHPSVEKAVLCEIDEEVVKECKKHLP
HHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC
EISNNALEDSRCEVFIGDGIKYVNEHKNEFDVVIIDSTDPFGAAEGLFGGSFYGKISECL
HHCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TEDGIFVAQTETPFYLPEIVKRVYDDSKAVFPVTKLFMAAIPTYPSGYWSFTIGSKKHDP
CCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
EKAPVPEVLPFETKYYTPRLHSASFVLPKFVENLIFANISE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLWLKEGQISDAVMTYKIKETLVRKKTEYQDLAIVDTYALGRMLVLDGIVQTSIKDEYV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
YHEMITHIPLYTHPNPKKVLVVGGGDGGTIREILKHPSVEKAVLCEIDEEVVKECKKHLP
HHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCC
EISNNALEDSRCEVFIGDGIKYVNEHKNEFDVVIIDSTDPFGAAEGLFGGSFYGKISECL
HHCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TEDGIFVAQTETPFYLPEIVKRVYDDSKAVFPVTKLFMAAIPTYPSGYWSFTIGSKKHDP
CCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
EKAPVPEVLPFETKYYTPRLHSASFVLPKFVENLIFANISE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129