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The map label for this gene is pbpA

Identifier: 28211843

GI number: 28211843

Start: 2362578

End: 2364950

Strand: Direct

Name: pbpA

Synonym: CTC02242

Alternate gene names: 28211843

Gene position: 2362578-2364950 (Clockwise)

Preceding gene: 28211841

Following gene: 28211850

Centisome position: 84.4

GC content: 32.32

Gene sequence:

>2373_bases
ATGGCTAATGTTAGAAAGCGAAGAAAGAAAAAAAATGAACATAAAGCCCTGAGATTAACCTTTATTACTTTGCTTATGGT
CTTTTTATTTTCTTGTGTTGCAGCAGCTGGAGTAGGTCTGGCTATGATAAAAGCAGCCCCCCCTTTAGACGTAGATAAGG
TTCTAAATCCAAGCGAACCTTCTGTTATATATGACGATAAGAACAAATTGGTTGACACAGTTATATCTGACACTTATAGA
ACAATAGTTTCTTATGAAGACGTTCCTGACAATTTAAAAAATGCCTTTATTAGTATAGAAGATGAGCGTTTTTTCAATCA
TAGGGGGATAGATTATAAAAGAGTATTTGGAGCTTTCTTTAGAAATATTTCTAATAAATTAAAGGGTAAGTCTGCACTTC
AAGGTGCCTCTACTATAACCCAACAACTTGTAAGAAATACATTACTTTCTCAAGAGGTTAGAATTAAAAGAAAAGTTCAA
GAAATGTATTTATCTATTCAATTAGAAAAGAAAGTTAGCAAAGAGCAAATCTTAGAAGCTTATATGAATACTATACCTTT
GGGAGGAAGTGCTTATGGTATAGAAGCAGCATCTAAACAATATTTTGGTAAAAGTGTTAAAGATTTAAATTTAATTGAAA
GTGCATTTATCGCAGGTCTGCCTCAAAGTCCATCTACTTTTTATAATGCGGCAATGAGTCAAAAAAATACAGAAAGATAT
ATTAATAGAACTAAATTAGTATTAGGTAAAATGCGTGAACATAATTATATATCAAAAGAAGATTTCGATAAATCAATGGC
GTATATAGACAAAAACAAAATACCTCTTAAGACCTCTAATATAAATATAAGCAGATTAAACTATGAGTGGTTTTCTAGAG
AAATTATTAAACAAGTAAAAAAGGATCTAATGAATGAATATAAAATAAGTCCCACAGAAGCAGATAAGACAATAATGTAT
GGTGGTTTAAAAATATATGGTACTATGGATAAATCCCTACAAGACTTTTCCCAAAATACTTTAGATAATTTAGATGGTAT
TTTGGGAATTAATTCAAAGGATTATAGTGGTATAATTCAACCTGAAGCATCTGTATCTATAGTGGATTACAAAACTGGTA
ATGTAAAAGTCCTTATCGGTGGAAGAGGTAAACAACCTCCTTTATCCTTTAATAGGGCTACAGAATTTTATAGAGCTCCA
GGTTCTACTATAAAACCATTAACTGTTTATGGCCCTGCTATTGATACCAAAACATCCACTGCCGCTAGCTCTTACAATGA
TGCTCCAGTTCCCGAGGAAATAGGTAAACTTTATGATAAAGAACCTTATAATCCTAAAAACAGTCCAAATATATATGAAG
GCAAAATGACATTAAGAGAGGCTTTAATGAAATCTAAAAATGTTATTTCCGTAAGAATTGAGCATGAATTAGGATTAAAA
ACTGGTGCTGAGTATGGTAAAAAATTTGGATTAACTATTGATGACTCAATGGATGGAACCAGTATGGCTGCTCTTTCCCT
AGGTCAGCTAAGTGCTAAGACAGGTGTAAGTGGTACTAATACTTTAGGTATGGCAGCAGCTTACGGTGTCTTTGGTAATA
AAGGTGCTCTATCTAAACCTGTAGTATACAAAAAGGTAGTAGATAGAACTGGAAAAGTACTATTAGAAAATAAATATGCA
TCTTCTAAGGTTATGTCTCCGGAAGCTGCCTATATACTTTATGACTTACTAAAAGGACCTGTAAGTTATACTCCAGGTGC
TACTGGAATGAAAGCTAATTTTGGTCCAATGGCTAGAGGTAAAACTGGTACAAGTAATCAGTCTAGTGATTTATGGTTTG
CTGGTCTTTCACCTTATTACTCAGCAGCTGTGTGGATAGGGAAAGATGACTATTCTCCATTTACAAATGAATTTGGTAGA
TATATAGGAAGTAGCGATGCCGCCTTAATTTGGAAACTTATAATGGGTGAGGCTCATAAAAATTTAGAGTATAAAACCAT
AGAAAAACCTGCTGGTGTAACAGAAGCATATGTGTGTAGTAAATCAGGTAAAATTCCTTCCTCTTCTTGTCCAAGGGATA
GTATTAAATTAGAATACTTTATAGAAGGAACTATACCTGGAGAGATATGTGATTATCATACTGGCATATTTAACAATAAT
AAAGATAAAGATGATGATGATGATGATAAAGACAAGGACAAAGAAGATGAAGAAGAAAACAAAGATGAAAAGAACGAGGA
TAAGAAAGAAGCTAAAGATAATACCAAAAATAAAGATAAGGATAAAAAGAAAGATAACGATAGAAAGATAGATATGGATA
AAAAACCAGATAGCAGTAAGAGAAAAAGAAAAATGATAAAACCACAAATATAA

Upstream 100 bases:

>100_bases
ACTTTTTAGTAATTCATCAATATTTAAACATTTTATAAAAACTATATTGTATGATATAATTAGTTAAAAATTAAATGATA
GTATTTTCAGGAGGAAAATT

Downstream 100 bases:

>100_bases
TTTAATATATTTAAAAGAGTAAATTCCACTGGGAATTTACTCTTTTGTTTTTGCATTAAATATTACTGCCATTAAATATC
CAAAAACTATTGCAGCAGTT

Product: multimodular transpeptidase-transglycosylase

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 790; Mature: 789

Protein sequence:

>790_residues
MANVRKRRKKKNEHKALRLTFITLLMVFLFSCVAAAGVGLAMIKAAPPLDVDKVLNPSEPSVIYDDKNKLVDTVISDTYR
TIVSYEDVPDNLKNAFISIEDERFFNHRGIDYKRVFGAFFRNISNKLKGKSALQGASTITQQLVRNTLLSQEVRIKRKVQ
EMYLSIQLEKKVSKEQILEAYMNTIPLGGSAYGIEAASKQYFGKSVKDLNLIESAFIAGLPQSPSTFYNAAMSQKNTERY
INRTKLVLGKMREHNYISKEDFDKSMAYIDKNKIPLKTSNINISRLNYEWFSREIIKQVKKDLMNEYKISPTEADKTIMY
GGLKIYGTMDKSLQDFSQNTLDNLDGILGINSKDYSGIIQPEASVSIVDYKTGNVKVLIGGRGKQPPLSFNRATEFYRAP
GSTIKPLTVYGPAIDTKTSTAASSYNDAPVPEEIGKLYDKEPYNPKNSPNIYEGKMTLREALMKSKNVISVRIEHELGLK
TGAEYGKKFGLTIDDSMDGTSMAALSLGQLSAKTGVSGTNTLGMAAAYGVFGNKGALSKPVVYKKVVDRTGKVLLENKYA
SSKVMSPEAAYILYDLLKGPVSYTPGATGMKANFGPMARGKTGTSNQSSDLWFAGLSPYYSAAVWIGKDDYSPFTNEFGR
YIGSSDAALIWKLIMGEAHKNLEYKTIEKPAGVTEAYVCSKSGKIPSSSCPRDSIKLEYFIEGTIPGEICDYHTGIFNNN
KDKDDDDDDKDKDKEDEEENKDEKNEDKKEAKDNTKNKDKDKKKDNDRKIDMDKKPDSSKRKRKMIKPQI

Sequences:

>Translated_790_residues
MANVRKRRKKKNEHKALRLTFITLLMVFLFSCVAAAGVGLAMIKAAPPLDVDKVLNPSEPSVIYDDKNKLVDTVISDTYR
TIVSYEDVPDNLKNAFISIEDERFFNHRGIDYKRVFGAFFRNISNKLKGKSALQGASTITQQLVRNTLLSQEVRIKRKVQ
EMYLSIQLEKKVSKEQILEAYMNTIPLGGSAYGIEAASKQYFGKSVKDLNLIESAFIAGLPQSPSTFYNAAMSQKNTERY
INRTKLVLGKMREHNYISKEDFDKSMAYIDKNKIPLKTSNINISRLNYEWFSREIIKQVKKDLMNEYKISPTEADKTIMY
GGLKIYGTMDKSLQDFSQNTLDNLDGILGINSKDYSGIIQPEASVSIVDYKTGNVKVLIGGRGKQPPLSFNRATEFYRAP
GSTIKPLTVYGPAIDTKTSTAASSYNDAPVPEEIGKLYDKEPYNPKNSPNIYEGKMTLREALMKSKNVISVRIEHELGLK
TGAEYGKKFGLTIDDSMDGTSMAALSLGQLSAKTGVSGTNTLGMAAAYGVFGNKGALSKPVVYKKVVDRTGKVLLENKYA
SSKVMSPEAAYILYDLLKGPVSYTPGATGMKANFGPMARGKTGTSNQSSDLWFAGLSPYYSAAVWIGKDDYSPFTNEFGR
YIGSSDAALIWKLIMGEAHKNLEYKTIEKPAGVTEAYVCSKSGKIPSSSCPRDSIKLEYFIEGTIPGEICDYHTGIFNNN
KDKDDDDDDKDKDKEDEEENKDEKNEDKKEAKDNTKNKDKDKKKDNDRKIDMDKKPDSSKRKRKMIKPQI
>Mature_789_residues
ANVRKRRKKKNEHKALRLTFITLLMVFLFSCVAAAGVGLAMIKAAPPLDVDKVLNPSEPSVIYDDKNKLVDTVISDTYRT
IVSYEDVPDNLKNAFISIEDERFFNHRGIDYKRVFGAFFRNISNKLKGKSALQGASTITQQLVRNTLLSQEVRIKRKVQE
MYLSIQLEKKVSKEQILEAYMNTIPLGGSAYGIEAASKQYFGKSVKDLNLIESAFIAGLPQSPSTFYNAAMSQKNTERYI
NRTKLVLGKMREHNYISKEDFDKSMAYIDKNKIPLKTSNINISRLNYEWFSREIIKQVKKDLMNEYKISPTEADKTIMYG
GLKIYGTMDKSLQDFSQNTLDNLDGILGINSKDYSGIIQPEASVSIVDYKTGNVKVLIGGRGKQPPLSFNRATEFYRAPG
STIKPLTVYGPAIDTKTSTAASSYNDAPVPEEIGKLYDKEPYNPKNSPNIYEGKMTLREALMKSKNVISVRIEHELGLKT
GAEYGKKFGLTIDDSMDGTSMAALSLGQLSAKTGVSGTNTLGMAAAYGVFGNKGALSKPVVYKKVVDRTGKVLLENKYAS
SKVMSPEAAYILYDLLKGPVSYTPGATGMKANFGPMARGKTGTSNQSSDLWFAGLSPYYSAAVWIGKDDYSPFTNEFGRY
IGSSDAALIWKLIMGEAHKNLEYKTIEKPAGVTEAYVCSKSGKIPSSSCPRDSIKLEYFIEGTIPGEICDYHTGIFNNNK
DKDDDDDDKDKDKEDEEENKDEKNEDKKEAKDNTKNKDKDKKKDNDRKIDMDKKPDSSKRKRKMIKPQI

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI87082258, Length=284, Percent_Identity=35.2112676056338, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1786343, Length=562, Percent_Identity=27.4021352313167, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788867, Length=685, Percent_Identity=26.1313868613139, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789601, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPA_CLOTE (Q891X1)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_782787.1
- ProteinModelPortal:   Q891X1
- SMR:   Q891X1
- GeneID:   1059275
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC02242
- NMPDR:   fig|212717.1.peg.2009
- HOGENOM:   HBG672432
- OMA:   KENETHT
- BioCyc:   CTET212717:CTC_02242-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 88212; Mature: 88081

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 402-402

Signals:

None

Transmembrane regions:

HASH(0xe96c16c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVRKRRKKKNEHKALRLTFITLLMVFLFSCVAAAGVGLAMIKAAPPLDVDKVLNPSEP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHCCCCCCC
SVIYDDKNKLVDTVISDTYRTIVSYEDVPDNLKNAFISIEDERFFNHRGIDYKRVFGAFF
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEHHHHHCCCCCHHHHHHHHHH
RNISNKLKGKSALQGASTITQQLVRNTLLSQEVRIKRKVQEMYLSIQLEKKVSKEQILEA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YMNTIPLGGSAYGIEAASKQYFGKSVKDLNLIESAFIAGLPQSPSTFYNAAMSQKNTERY
HHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
INRTKLVLGKMREHNYISKEDFDKSMAYIDKNKIPLKTSNINISRLNYEWFSREIIKQVK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCEEEEECCHHHHHHHHHHHHH
KDLMNEYKISPTEADKTIMYGGLKIYGTMDKSLQDFSQNTLDNLDGILGINSKDYSGIIQ
HHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEC
PEASVSIVDYKTGNVKVLIGGRGKQPPLSFNRATEFYRAPGSTIKPLTVYGPAIDTKTST
CCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
AASSYNDAPVPEEIGKLYDKEPYNPKNSPNIYEGKMTLREALMKSKNVISVRIEHELGLK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCH
TGAEYGKKFGLTIDDSMDGTSMAALSLGQLSAKTGVSGTNTLGMAAAYGVFGNKGALSKP
HHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCC
VVYKKVVDRTGKVLLENKYASSKVMSPEAAYILYDLLKGPVSYTPGATGMKANFGPMARG
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KTGTSNQSSDLWFAGLSPYYSAAVWIGKDDYSPFTNEFGRYIGSSDAALIWKLIMGEAHK
CCCCCCCCCCEEEECCCCHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NLEYKTIEKPAGVTEAYVCSKSGKIPSSSCPRDSIKLEYFIEGTIPGEICDYHTGIFNNN
CCCEEECCCCCCCCHHEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCC
KDKDDDDDDKDKDKEDEEENKDEKNEDKKEAKDNTKNKDKDKKKDNDRKIDMDKKPDSSK
CCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCHHH
RKRKMIKPQI
HHHHCCCCCH
>Mature Secondary Structure 
ANVRKRRKKKNEHKALRLTFITLLMVFLFSCVAAAGVGLAMIKAAPPLDVDKVLNPSEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHCCCCCCC
SVIYDDKNKLVDTVISDTYRTIVSYEDVPDNLKNAFISIEDERFFNHRGIDYKRVFGAFF
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCEEEEEHHHHHCCCCCHHHHHHHHHH
RNISNKLKGKSALQGASTITQQLVRNTLLSQEVRIKRKVQEMYLSIQLEKKVSKEQILEA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YMNTIPLGGSAYGIEAASKQYFGKSVKDLNLIESAFIAGLPQSPSTFYNAAMSQKNTERY
HHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
INRTKLVLGKMREHNYISKEDFDKSMAYIDKNKIPLKTSNINISRLNYEWFSREIIKQVK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCEEEEECCHHHHHHHHHHHHH
KDLMNEYKISPTEADKTIMYGGLKIYGTMDKSLQDFSQNTLDNLDGILGINSKDYSGIIQ
HHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEC
PEASVSIVDYKTGNVKVLIGGRGKQPPLSFNRATEFYRAPGSTIKPLTVYGPAIDTKTST
CCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
AASSYNDAPVPEEIGKLYDKEPYNPKNSPNIYEGKMTLREALMKSKNVISVRIEHELGLK
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCH
TGAEYGKKFGLTIDDSMDGTSMAALSLGQLSAKTGVSGTNTLGMAAAYGVFGNKGALSKP
HHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCC
VVYKKVVDRTGKVLLENKYASSKVMSPEAAYILYDLLKGPVSYTPGATGMKANFGPMARG
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KTGTSNQSSDLWFAGLSPYYSAAVWIGKDDYSPFTNEFGRYIGSSDAALIWKLIMGEAHK
CCCCCCCCCCEEEECCCCHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NLEYKTIEKPAGVTEAYVCSKSGKIPSSSCPRDSIKLEYFIEGTIPGEICDYHTGIFNNN
CCCEEECCCCCCCCHHEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCC
KDKDDDDDDKDKDKEDEEENKDEKNEDKKEAKDNTKNKDKDKKKDNDRKIDMDKKPDSSK
CCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCHHH
RKRKMIKPQI
HHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12552129