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The map label for this gene is yfnB [H]

Identifier: 28211556

GI number: 28211556

Start: 2051876

End: 2052550

Strand: Direct

Name: yfnB [H]

Synonym: CTC01925

Alternate gene names: 28211556

Gene position: 2051876-2052550 (Clockwise)

Preceding gene: 28211555

Following gene: 28211563

Centisome position: 73.3

GC content: 33.04

Gene sequence:

>675_bases
ATGTATGATATTATTTTGATGGACTTAGATAATACTATTTTGGATTTTGATGTTGCTGAAAAAGACAGTTTCAGAAAAAT
CATTGAATCAACAGGGCTAAACTATACAGATGAGTTATTGCAGCAATACAAAAAAATAAACAGATCTTTATGGAATCGCC
TCGAACAAGGAAAACTTTCAAAAGAAGTTGTATTGAATACGCGTTTCAGTGAGTTTTTTAAGCTTTATGATATTCAAGTT
GATGGTAGGAATATAGAAAAAAGGTACAGATTTTACCTTGATAATTCTTCTTCGCTCATTCACAATGCAGAATACACCTT
AACAGAACTGAAGATAATGGGGAAAAAAATTTATTCAGCATCTAACGGTGTATATTCAACGCAAATCAAGCGCCTGTCAA
ATGCTGGAATAATTCACTTATTTGATGGGCATTTTATCTCTGACAAAATAAAATATGAAAAGCCTTCCCCTTATTTCTTT
GACTTCTGTATCAAAAACATATGTGGAGTTCCTGATAGTTCCATAATCATGGTAGGAGATAGTCCTACTTCTGATGTGCA
GGGAGCAGTTAATGCCGGAATTGATTCCTGTTTCTATCAACATAACAAAAGTGTTGAATGTGCCTATGCTAAATATACCA
TACATGATATTTCCGAACTCTTAGATATAGTGTAG

Upstream 100 bases:

>100_bases
TATAAGAGAAGGAACTATCATTAGCTTTTTCGTACTCTCTAGGTTAATGGGTTTCTCATACTATTTTCATAAAAAACATT
TATAGAAGGGGTTATAGATT

Downstream 100 bases:

>100_bases
CTTATTTGTATTAAAAACGATTGTATCTCAATGCACAATTTTATAAAAGCCCTTGAGGCTTCAATTTCACTCAAGGGCTT
AAATTCATTGATATTACTCA

Product: 2-haloalkanoic acid dehalogenase I

Products: (R)-2-hydroxyacid; halide

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MYDIILMDLDNTILDFDVAEKDSFRKIIESTGLNYTDELLQQYKKINRSLWNRLEQGKLSKEVVLNTRFSEFFKLYDIQV
DGRNIEKRYRFYLDNSSSLIHNAEYTLTELKIMGKKIYSASNGVYSTQIKRLSNAGIIHLFDGHFISDKIKYEKPSPYFF
DFCIKNICGVPDSSIIMVGDSPTSDVQGAVNAGIDSCFYQHNKSVECAYAKYTIHDISELLDIV

Sequences:

>Translated_224_residues
MYDIILMDLDNTILDFDVAEKDSFRKIIESTGLNYTDELLQQYKKINRSLWNRLEQGKLSKEVVLNTRFSEFFKLYDIQV
DGRNIEKRYRFYLDNSSSLIHNAEYTLTELKIMGKKIYSASNGVYSTQIKRLSNAGIIHLFDGHFISDKIKYEKPSPYFF
DFCIKNICGVPDSSIIMVGDSPTSDVQGAVNAGIDSCFYQHNKSVECAYAKYTIHDISELLDIV
>Mature_224_residues
MYDIILMDLDNTILDFDVAEKDSFRKIIESTGLNYTDELLQQYKKINRSLWNRLEQGKLSKEVVLNTRFSEFFKLYDIQV
DGRNIEKRYRFYLDNSSSLIHNAEYTLTELKIMGKKIYSASNGVYSTQIKRLSNAGIIHLFDGHFISDKIKYEKPSPYFF
DFCIKNICGVPDSSIIMVGDSPTSDVQGAVNAGIDSCFYQHNKSVECAYAKYTIHDISELLDIV

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. YjjG family [H]

Homologues:

Organism=Escherichia coli, GI1790833, Length=222, Percent_Identity=25.2252252252252, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR011951 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.8.1.2

Molecular weight: Translated: 25831; Mature: 25831

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDIILMDLDNTILDFDVAEKDSFRKIIESTGLNYTDELLQQYKKINRSLWNRLEQGKLS
CCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KEVVLNTRFSEFFKLYDIQVDGRNIEKRYRFYLDNSSSLIHNAEYTLTELKIMGKKIYSA
HHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHC
SNGVYSTQIKRLSNAGIIHLFDGHFISDKIKYEKPSPYFFDFCIKNICGVPDSSIIMVGD
CCCCHHHHHHHHCCCCEEEEECCEEECCEECCCCCCCHHHHHHHHHHCCCCCCCEEEECC
SPTSDVQGAVNAGIDSCFYQHNKSVECAYAKYTIHDISELLDIV
CCCCHHHHHHHCCHHHHHHHCCCCEEEEEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MYDIILMDLDNTILDFDVAEKDSFRKIIESTGLNYTDELLQQYKKINRSLWNRLEQGKLS
CCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KEVVLNTRFSEFFKLYDIQVDGRNIEKRYRFYLDNSSSLIHNAEYTLTELKIMGKKIYSA
HHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHC
SNGVYSTQIKRLSNAGIIHLFDGHFISDKIKYEKPSPYFFDFCIKNICGVPDSSIIMVGD
CCCCHHHHHHHHCCCCEEEEECCEEECCEECCCCCCCHHHHHHHHHHCCCCCCCEEEECC
SPTSDVQGAVNAGIDSCFYQHNKSVECAYAKYTIHDISELLDIV
CCCCHHHHHHHCCHHHHHHHCCCCEEEEEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]