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The map label for this gene is nikB [H]

Identifier: 28211079

GI number: 28211079

Start: 1513648

End: 1514598

Strand: Direct

Name: nikB [H]

Synonym: CTC01401

Alternate gene names: 28211079

Gene position: 1513648-1514598 (Clockwise)

Preceding gene: 28211078

Following gene: 28211080

Centisome position: 54.07

GC content: 30.07

Gene sequence:

>951_bases
ATGAAAGAATCCTTAAAGTATTTATTTAAAAAATTAATTCATGCAATTCCTGTACTTATAGGTGTTTCACTTATATCCTT
TATGTTAGGAATATTAGCACCGGGAGATCCGGCGCTTCAAATTCTTACTAAAGACTCTATAAGTCAGCCTACTAAAGAAG
AAATCTATACCTTAAGGGAAGATATGGGCTTAAATGATCCAATATTAACTCAATATGGAAGATGGGCTAAAAAAGCTATA
AAGTGTGATCTTGGAAAATCTTATATAACGGACCAACCTGTATTTGATGAATTAGTTAAGAGAATTCCTATAACCTTTTT
AATATCTATATTTGCAGTAACTATTGCTGCACTATTGGGTATACCCTTGGGAATTATTATGGCAGTAAAAAATAATAGTC
ACATTGATAATGTAGGAAGAATTATAGCATTAATTTTTATTTCCATTCCAGGATTTTGGTTTGCATTTATTTTAATAACC
ATATTTTCAGAAAAACTTAAAATTCTCCCAACCAATGGTGTAGAAGGATTTAAAAATCTTATTATGCCCTCTTTTATATT
AGCATCAGGAACCATAGGAGTAATAATGAGACTTACAAGAGCTACTGTTTTAAAGGAACTTAATAAAAATTACATAATAA
CCGCAGAATCAAAGGGACTTTCTTCTAAAACCATAGTTATAAAACATGCTTTCTTAAATTCCCTATTACCAGTAATAACA
CTTATAGGTAATTATTTTGCAGGAATCCTTGGAGGTACTGCTGTAATTGAAACCATATTTGCCATTCCTGGTATGGGTAG
CTTTGCTATTAATGGAATATTTAATAGAGATTATCCTGTAATTCAAGGCTATGTTCTTTTTACAGGCATTATCTATATAA
TCTTTAATTTAATAATAGATTTATCCTACCTTGTTTTAAACCCTAAAATAAGGCTTGGAGGTGAATCTTAA

Upstream 100 bases:

>100_bases
ATTTAGTGAAATGAATTTAATTGTATCTAATAAAGAAGTTACTGGACACAATGCATTTATATATGGAACCACTCTTCCAG
AACTTCAATGGAGTAAATAA

Downstream 100 bases:

>100_bases
TATGAAAATACATAAATTTGCTGTTATTACCTTAGGTACAATTCTATTTTTAAGTATATTTGCTCCATTAATTTCTCCAT
ACGATCCTATAAAAGTTGAT

Product: transport system permease, nickel or dipeptide

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKESLKYLFKKLIHAIPVLIGVSLISFMLGILAPGDPALQILTKDSISQPTKEEIYTLREDMGLNDPILTQYGRWAKKAI
KCDLGKSYITDQPVFDELVKRIPITFLISIFAVTIAALLGIPLGIIMAVKNNSHIDNVGRIIALIFISIPGFWFAFILIT
IFSEKLKILPTNGVEGFKNLIMPSFILASGTIGVIMRLTRATVLKELNKNYIITAESKGLSSKTIVIKHAFLNSLLPVIT
LIGNYFAGILGGTAVIETIFAIPGMGSFAINGIFNRDYPVIQGYVLFTGIIYIIFNLIIDLSYLVLNPKIRLGGES

Sequences:

>Translated_316_residues
MKESLKYLFKKLIHAIPVLIGVSLISFMLGILAPGDPALQILTKDSISQPTKEEIYTLREDMGLNDPILTQYGRWAKKAI
KCDLGKSYITDQPVFDELVKRIPITFLISIFAVTIAALLGIPLGIIMAVKNNSHIDNVGRIIALIFISIPGFWFAFILIT
IFSEKLKILPTNGVEGFKNLIMPSFILASGTIGVIMRLTRATVLKELNKNYIITAESKGLSSKTIVIKHAFLNSLLPVIT
LIGNYFAGILGGTAVIETIFAIPGMGSFAINGIFNRDYPVIQGYVLFTGIIYIIFNLIIDLSYLVLNPKIRLGGES
>Mature_316_residues
MKESLKYLFKKLIHAIPVLIGVSLISFMLGILAPGDPALQILTKDSISQPTKEEIYTLREDMGLNDPILTQYGRWAKKAI
KCDLGKSYITDQPVFDELVKRIPITFLISIFAVTIAALLGIPLGIIMAVKNNSHIDNVGRIIALIFISIPGFWFAFILIT
IFSEKLKILPTNGVEGFKNLIMPSFILASGTIGVIMRLTRATVLKELNKNYIITAESKGLSSKTIVIKHAFLNSLLPVIT
LIGNYFAGILGGTAVIETIFAIPGMGSFAINGIFNRDYPVIQGYVLFTGIIYIIFNLIIDLSYLVLNPKIRLGGES

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=307, Percent_Identity=36.1563517915309, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1787053, Length=307, Percent_Identity=35.5048859934853, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=32.5443786982249, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=35.8064516129032, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1787761, Length=328, Percent_Identity=35.3658536585366, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=30.5660377358491, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788504, Length=365, Percent_Identity=26.3013698630137, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34764; Mature: 34764

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESLKYLFKKLIHAIPVLIGVSLISFMLGILAPGDPALQILTKDSISQPTKEEIYTLRE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHCCCCCCHHHHHHHHH
DMGLNDPILTQYGRWAKKAIKCDLGKSYITDQPVFDELVKRIPITFLISIFAVTIAALLG
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IPLGIIMAVKNNSHIDNVGRIIALIFISIPGFWFAFILITIFSEKLKILPTNGVEGFKNL
CHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
IMPSFILASGTIGVIMRLTRATVLKELNKNYIITAESKGLSSKTIVIKHAFLNSLLPVIT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LIGNYFAGILGGTAVIETIFAIPGMGSFAINGIFNRDYPVIQGYVLFTGIIYIIFNLIID
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSYLVLNPKIRLGGES
HHHHHCCCEEECCCCC
>Mature Secondary Structure
MKESLKYLFKKLIHAIPVLIGVSLISFMLGILAPGDPALQILTKDSISQPTKEEIYTLRE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHCCCCCCHHHHHHHHH
DMGLNDPILTQYGRWAKKAIKCDLGKSYITDQPVFDELVKRIPITFLISIFAVTIAALLG
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IPLGIIMAVKNNSHIDNVGRIIALIFISIPGFWFAFILITIFSEKLKILPTNGVEGFKNL
CHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHH
IMPSFILASGTIGVIMRLTRATVLKELNKNYIITAESKGLSSKTIVIKHAFLNSLLPVIT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LIGNYFAGILGGTAVIETIFAIPGMGSFAINGIFNRDYPVIQGYVLFTGIIYIIFNLIID
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LSYLVLNPKIRLGGES
HHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]