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The map label for this gene is truB

Identifier: 28210964

GI number: 28210964

Start: 1370684

End: 1371553

Strand: Direct

Name: truB

Synonym: CTC01278

Alternate gene names: 28210964

Gene position: 1370684-1371553 (Clockwise)

Preceding gene: 28210963

Following gene: 28210965

Centisome position: 48.97

GC content: 28.51

Gene sequence:

>870_bases
ATGAATGGTGTAATAAATGTATATAAGCCTCAAAATATTACCTCTTTTGATGTGGTAAGAATTATTAAAAAAATAAGTAG
AATAAAAAAAGTAGGGCATACAGGTACATTAGATCCAATGGCTACAGGGGTTTTACCTATATGCTTAGGTGGTTCTACCA
AAATTGTTGATTTTATTATGAATGAACACAAAGAGTATAGAGCTAAACTAAAATTAGGATTAATCACAGATACTTATGAT
AGAGAAGGAAAAGTACTGAAAGAAGAAGATGCATCCAAAATTTTAGAGGAGGAAGTTGTAAACTGTATAAATTCTTTTAA
AGGGGAAATTATACAAATTCCTCCTATGTACTCTGCTATAAAAATTAAAGGGGAAAGATTATACAATCTTGCAAGAAAAG
GAATAGAGGTTGAAAGAGAAGGTAGAAAAATAAATATCTATAATATAGAAGTGCTAAAGGTGAATCTTCCCTATGTAGAA
TTTAAAGTAAATTGTTCTAAGGGAACTTACATAAGAAGTCTTTGCTATGATATTGGTAACAAATTAGGAATGGGGGCAAC
TATGTGGGAATTGGAGAGAACTAAAACAGGAAATTTCTCTATTGAAAACTCTATAAACTTAGAAGATATAAATGAAGAAA
ATATACAAGAATTTATAATTCCTGCAGAAAAAGCTCTAAGTAAATATGAAAGAATAGAAATAGATGAATACTTTTCAAGA
CTTCTAAAAAATGGAGTAAAAGTAAAAGATAAAAGGCTTTTAGATAAAATAAAAGCAAATGATATTTTAAGAGTTTACCA
AGAAGATAAATTCATAGGGTTAGGACAAAAAACTAAAGAGGGGTTTAAGATCGTTAAATTACTTGTATAG

Upstream 100 bases:

>100_bases
GAGGCGGACATATAAGGGCTGCTGGAGCTTCTGTTAAGGGAAAAACCTTAGAAGAAGTAAAAAATATAGCAATAGATTTA
ATAAAAAAAGAGCTGATGAA

Downstream 100 bases:

>100_bases
GAGTAATGAATATGGATATAATAGAAGAAGAGATAAAAACAAAGTATAACCTTGATATTTATGTAGCTTTAGGAAGTTTT
GATGGACTGCACTTAGGACA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MNGVINVYKPQNITSFDVVRIIKKISRIKKVGHTGTLDPMATGVLPICLGGSTKIVDFIMNEHKEYRAKLKLGLITDTYD
REGKVLKEEDASKILEEEVVNCINSFKGEIIQIPPMYSAIKIKGERLYNLARKGIEVEREGRKINIYNIEVLKVNLPYVE
FKVNCSKGTYIRSLCYDIGNKLGMGATMWELERTKTGNFSIENSINLEDINEENIQEFIIPAEKALSKYERIEIDEYFSR
LLKNGVKVKDKRLLDKIKANDILRVYQEDKFIGLGQKTKEGFKIVKLLV

Sequences:

>Translated_289_residues
MNGVINVYKPQNITSFDVVRIIKKISRIKKVGHTGTLDPMATGVLPICLGGSTKIVDFIMNEHKEYRAKLKLGLITDTYD
REGKVLKEEDASKILEEEVVNCINSFKGEIIQIPPMYSAIKIKGERLYNLARKGIEVEREGRKINIYNIEVLKVNLPYVE
FKVNCSKGTYIRSLCYDIGNKLGMGATMWELERTKTGNFSIENSINLEDINEENIQEFIIPAEKALSKYERIEIDEYFSR
LLKNGVKVKDKRLLDKIKANDILRVYQEDKFIGLGQKTKEGFKIVKLLV
>Mature_289_residues
MNGVINVYKPQNITSFDVVRIIKKISRIKKVGHTGTLDPMATGVLPICLGGSTKIVDFIMNEHKEYRAKLKLGLITDTYD
REGKVLKEEDASKILEEEVVNCINSFKGEIIQIPPMYSAIKIKGERLYNLARKGIEVEREGRKINIYNIEVLKVNLPYVE
FKVNCSKGTYIRSLCYDIGNKLGMGATMWELERTKTGNFSIENSINLEDINEENIQEFIIPAEKALSKYERIEIDEYFSR
LLKNGVKVKDKRLLDKIKANDILRVYQEDKFIGLGQKTKEGFKIVKLLV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=253, Percent_Identity=35.1778656126482, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=28.3524904214559, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4503337, Length=261, Percent_Identity=28.3524904214559, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI7661556, Length=194, Percent_Identity=25.2577319587629, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI2367200, Length=304, Percent_Identity=35.1973684210526, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17553978, Length=262, Percent_Identity=25.9541984732824, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=263, Percent_Identity=30.7984790874525, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=140, Percent_Identity=33.5714285714286, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364189, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364187, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364185, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364183, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62471759, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17975520, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CLOTE (Q895J5)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781908.1
- ProteinModelPortal:   Q895J5
- SMR:   Q895J5
- GeneID:   1059411
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01278
- NMPDR:   fig|212717.1.peg.1130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   Q895J5
- ProtClustDB:   PRK00130
- BioCyc:   CTET212717:CTC_01278-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33102; Mature: 33102

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGVINVYKPQNITSFDVVRIIKKISRIKKVGHTGTLDPMATGVLPICLGGSTKIVDFIM
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCHHHHHHHH
NEHKEYRAKLKLGLITDTYDREGKVLKEEDASKILEEEVVNCINSFKGEIIQIPPMYSAI
HHHHHHHHHEEEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEE
KIKGERLYNLARKGIEVEREGRKINIYNIEVLKVNLPYVEFKVNCSKGTYIRSLCYDIGN
EECHHHHHHHHHCCCEEECCCCEEEEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHCC
KLGMGATMWELERTKTGNFSIENSINLEDINEENIQEFIIPAEKALSKYERIEIDEYFSR
CCCCCCCHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
LLKNGVKVKDKRLLDKIKANDILRVYQEDKFIGLGQKTKEGFKIVKLLV
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHEEC
>Mature Secondary Structure
MNGVINVYKPQNITSFDVVRIIKKISRIKKVGHTGTLDPMATGVLPICLGGSTKIVDFIM
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCHHHHHHHH
NEHKEYRAKLKLGLITDTYDREGKVLKEEDASKILEEEVVNCINSFKGEIIQIPPMYSAI
HHHHHHHHHEEEEEEECCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEE
KIKGERLYNLARKGIEVEREGRKINIYNIEVLKVNLPYVEFKVNCSKGTYIRSLCYDIGN
EECHHHHHHHHHCCCEEECCCCEEEEEEEEEEEEECCEEEEEEECCCCHHHHHHHHHHCC
KLGMGATMWELERTKTGNFSIENSINLEDINEENIQEFIIPAEKALSKYERIEIDEYFSR
CCCCCCCHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
LLKNGVKVKDKRLLDKIKANDILRVYQEDKFIGLGQKTKEGFKIVKLLV
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129