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The map label for this gene is topA [H]

Identifier: 28210945

GI number: 28210945

Start: 1347856

End: 1349946

Strand: Direct

Name: topA [H]

Synonym: CTC01259

Alternate gene names: 28210945

Gene position: 1347856-1349946 (Clockwise)

Preceding gene: 28210944

Following gene: 28210946

Centisome position: 48.15

GC content: 29.56

Gene sequence:

>2091_bases
ATGATTATGGGACAAAAACTTATCATTGTGGAATCACCAGCAAAAGCAAAAACCATTGGAAAATACCTAGATAAAACTTA
TCATGTTGAAGCATCTATGGGACATATAAGAGATCTTCCAAAGAGTCAATTGGGAGTTGATATAGATAATAATTATGAAC
CCAAATATATAACTATTAGAGGAAAAGGAGAGCTTTTAACTAAAATAAAAAAACATGCTAAAAAATGCGATGAAATTTAT
TTGGCTACAGACCCTGATAGAGAGGGAGAAGCTATTTCTTGGCATTTATCTCAGGCACTAAAATTAGAGGATAATAAAAA
ATGTAGAATAGTATTTAATGAAATAACTAAAAGTGCAGTAAAAAATGCTATAAAGGAACCTAGAGAAATTGACATAGATT
TAGTAAATGCTCAGCAAGCTAGAAGGGTATTGGATAGACTAGTAGGATATAAAATTAGCCCAATTCTATGGAGAAAAGTA
AGATGGGGTTTAAGCGCAGGTAGAGTTCAATCTGTTGTATTAAAGATGATATGTGACAGAGAAAAAGAAATAGAAGAGTT
TATTCCTAAAGAATATTGGACTATTGAATGTAAACTAAATAAAGATAGAAAAGATTTTTTAGTTAAACTAATATCAAAGG
ATAATAAAAAAATTGAAATAAATACAGAAGAAGAAAGTAATAATATAATCAAAGAGCTTAAACAAGGTCAATTTACGGTA
GATAAGATAAAAAAGACCAACAAAAATAAAAATCCACTACCTCCATTTATAACAAGTACTCTACAGCAAGATGCTTATAG
AAAATTAAATTTTTCAACAAAGAAGACCATGTCTGTAGCTCAGCAACTTTATGAAGGAATTGATATAAAAGGACATGGAA
CCGTAGGTCTTATAACTTATATGAGAACAGATTCTGTTAGAATATCTAAAGAGGCACAAAATAAGGCTAGACAATTTTTA
GAAAATGTATATGGAAAAGAATATATACCAGAAAAGCCTAGAGATTATAAAGGTAAAAAAAATATTCAAGATGCCCATGA
GGCAATAAGACCTACAAATATAGAAATAACACCTAAAATTGCTTTAGAAAATTTAAATTCAGATCAATATAAATTATATT
CTTTAATCTGGAATAGATTTATGGCAAGTCAAATGGCAGCTTGTGTAATGGAAGTTAATTCTGTAGATGTAAAAAATAGT
GAATATATTTTCAGAGCTACAGGTTCAATTGTTAAATTCGATGGTTTCATGATAATTTATGAATATTTAAATGAAGACGA
AAAAGAAAATATAAATATTCCTAAGTTAGAAAAAGGAGAACTTCTTAAAGAAAAATCTATAGATAGCAAACAGCACTTTA
CACAACCACCAGGAAGATTTTCAGAAGCATCTTTAGTTAAAGTGTTGGAAGACAATGGAATAGGAAGACCAAGTACTTAT
GCTCCAATTATATCTACCTTATTAGAAAGAACCTATATAGAAAGGGAAAATAGAACTTTAAAATCTACAGAATTGGGAGT
TATAGTAAATAATATTATGGTAGAATACTTTAAACCAGTAGTTGATGCTGAATTTACAGCAGAAATGGAAAATAAACTAG
ATAGTATTGAAGAAGGGAATGAAAATTGGAAGAATACAGTAGATGAATTTTATAGTCCATTAAAAGAACTTATAGAAACT
GCAGAAAAAGAAGTGGAAAAGGTCACTTTAGAGGAAAAGGTTACAGACGTAAAATGCGAAAAATGTGGTAATAACATGGT
GGTTAAGCATGGAAGATTTGGTGATTTCTTAGCTTGTCCAGGATATCCAGAATGTAAGAATACTAAACCTATAGTTCACC
AACTAGATGTAAAATGTCCTAAATGCCATGGAGAAATAGTATTAAAAAAGAGCAAGAAAGGAAGAAAATTTTACGGGTGT
AGCAATTATCCAAAATGTGATTTTGTAAGTTGGTTTGAACCAGTAGAAAGAAAATGTGATGTTTGTGGTAACTATATGGT
AAAAAGATATACTAAAGCTAAAGGGGATTACATTGAGTGTTCAAATAAAGAATGTAAACACAAAATTATGCCAGAAAAAA
AGCAGGAATAG

Upstream 100 bases:

>100_bases
ATAACTTGCCTTTCTGGCAATTATTATGTTAGAATTTCAAATAGCATTTAAAATAATAGGAAATTTAGGGGGTGAAACTC
TGATAATATAAATATCAGAG

Downstream 100 bases:

>100_bases
GTATAAGTTTAAAAAAATCTTAAAAAAACAGTAGAAATGTTGAAATTTCACAATTTATATGATATTATAAATATTATTAA
AAGGGGAATAATTCTAAAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MIMGQKLIIVESPAKAKTIGKYLDKTYHVEASMGHIRDLPKSQLGVDIDNNYEPKYITIRGKGELLTKIKKHAKKCDEIY
LATDPDREGEAISWHLSQALKLEDNKKCRIVFNEITKSAVKNAIKEPREIDIDLVNAQQARRVLDRLVGYKISPILWRKV
RWGLSAGRVQSVVLKMICDREKEIEEFIPKEYWTIECKLNKDRKDFLVKLISKDNKKIEINTEEESNNIIKELKQGQFTV
DKIKKTNKNKNPLPPFITSTLQQDAYRKLNFSTKKTMSVAQQLYEGIDIKGHGTVGLITYMRTDSVRISKEAQNKARQFL
ENVYGKEYIPEKPRDYKGKKNIQDAHEAIRPTNIEITPKIALENLNSDQYKLYSLIWNRFMASQMAACVMEVNSVDVKNS
EYIFRATGSIVKFDGFMIIYEYLNEDEKENINIPKLEKGELLKEKSIDSKQHFTQPPGRFSEASLVKVLEDNGIGRPSTY
APIISTLLERTYIERENRTLKSTELGVIVNNIMVEYFKPVVDAEFTAEMENKLDSIEEGNENWKNTVDEFYSPLKELIET
AEKEVEKVTLEEKVTDVKCEKCGNNMVVKHGRFGDFLACPGYPECKNTKPIVHQLDVKCPKCHGEIVLKKSKKGRKFYGC
SNYPKCDFVSWFEPVERKCDVCGNYMVKRYTKAKGDYIECSNKECKHKIMPEKKQE

Sequences:

>Translated_696_residues
MIMGQKLIIVESPAKAKTIGKYLDKTYHVEASMGHIRDLPKSQLGVDIDNNYEPKYITIRGKGELLTKIKKHAKKCDEIY
LATDPDREGEAISWHLSQALKLEDNKKCRIVFNEITKSAVKNAIKEPREIDIDLVNAQQARRVLDRLVGYKISPILWRKV
RWGLSAGRVQSVVLKMICDREKEIEEFIPKEYWTIECKLNKDRKDFLVKLISKDNKKIEINTEEESNNIIKELKQGQFTV
DKIKKTNKNKNPLPPFITSTLQQDAYRKLNFSTKKTMSVAQQLYEGIDIKGHGTVGLITYMRTDSVRISKEAQNKARQFL
ENVYGKEYIPEKPRDYKGKKNIQDAHEAIRPTNIEITPKIALENLNSDQYKLYSLIWNRFMASQMAACVMEVNSVDVKNS
EYIFRATGSIVKFDGFMIIYEYLNEDEKENINIPKLEKGELLKEKSIDSKQHFTQPPGRFSEASLVKVLEDNGIGRPSTY
APIISTLLERTYIERENRTLKSTELGVIVNNIMVEYFKPVVDAEFTAEMENKLDSIEEGNENWKNTVDEFYSPLKELIET
AEKEVEKVTLEEKVTDVKCEKCGNNMVVKHGRFGDFLACPGYPECKNTKPIVHQLDVKCPKCHGEIVLKKSKKGRKFYGC
SNYPKCDFVSWFEPVERKCDVCGNYMVKRYTKAKGDYIECSNKECKHKIMPEKKQE
>Mature_696_residues
MIMGQKLIIVESPAKAKTIGKYLDKTYHVEASMGHIRDLPKSQLGVDIDNNYEPKYITIRGKGELLTKIKKHAKKCDEIY
LATDPDREGEAISWHLSQALKLEDNKKCRIVFNEITKSAVKNAIKEPREIDIDLVNAQQARRVLDRLVGYKISPILWRKV
RWGLSAGRVQSVVLKMICDREKEIEEFIPKEYWTIECKLNKDRKDFLVKLISKDNKKIEINTEEESNNIIKELKQGQFTV
DKIKKTNKNKNPLPPFITSTLQQDAYRKLNFSTKKTMSVAQQLYEGIDIKGHGTVGLITYMRTDSVRISKEAQNKARQFL
ENVYGKEYIPEKPRDYKGKKNIQDAHEAIRPTNIEITPKIALENLNSDQYKLYSLIWNRFMASQMAACVMEVNSVDVKNS
EYIFRATGSIVKFDGFMIIYEYLNEDEKENINIPKLEKGELLKEKSIDSKQHFTQPPGRFSEASLVKVLEDNGIGRPSTY
APIISTLLERTYIERENRTLKSTELGVIVNNIMVEYFKPVVDAEFTAEMENKLDSIEEGNENWKNTVDEFYSPLKELIET
AEKEVEKVTLEEKVTDVKCEKCGNNMVVKHGRFGDFLACPGYPECKNTKPIVHQLDVKCPKCHGEIVLKKSKKGRKFYGC
SNYPKCDFVSWFEPVERKCDVCGNYMVKRYTKAKGDYIECSNKECKHKIMPEKKQE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=613, Percent_Identity=24.6329526916803, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI4507635, Length=682, Percent_Identity=23.3137829912023, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1787529, Length=750, Percent_Identity=37.8666666666667, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1788061, Length=548, Percent_Identity=25.5474452554745, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI48994931, Length=147, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17555378, Length=520, Percent_Identity=23.8461538461538, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=502, Percent_Identity=23.7051792828685, Blast_Score=109, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=538, Percent_Identity=22.6765799256506, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585251, Length=632, Percent_Identity=25, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640096, Length=511, Percent_Identity=21.5264187866928, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80387; Mature: 80387

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMGQKLIIVESPAKAKTIGKYLDKTYHVEASMGHIRDLPKSQLGVDIDNNYEPKYITIR
CCCCCEEEEEECCHHHHHHHHHHCHHEEEECHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGELLTKIKKHAKKCDEIYLATDPDREGEAISWHLSQALKLEDNKKCRIVFNEITKSAV
CCHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEHHHHHCCCCCCCEEEEHHHHHHHHH
KNAIKEPREIDIDLVNAQQARRVLDRLVGYKISPILWRKVRWGLSAGRVQSVVLKMICDR
HHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
EKEIEEFIPKEYWTIECKLNKDRKDFLVKLISKDNKKIEINTEEESNNIIKELKQGQFTV
HHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCH
DKIKKTNKNKNPLPPFITSTLQQDAYRKLNFSTKKTMSVAQQLYEGIDIKGHGTVGLITY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSVRISKEAQNKARQFLENVYGKEYIPEKPRDYKGKKNIQDAHEAIRPTNIEITPKI
EECCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEHHH
ALENLNSDQYKLYSLIWNRFMASQMAACVMEVNSVDVKNSEYIFRATGSIVKFDGFMIIY
HEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCEEEE
EYLNEDEKENINIPKLEKGELLKEKSIDSKQHFTQPPGRFSEASLVKVLEDNGIGRPSTY
HHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCH
APIISTLLERTYIERENRTLKSTELGVIVNNIMVEYFKPVVDAEFTAEMENKLDSIEEGN
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
ENWKNTVDEFYSPLKELIETAEKEVEKVTLEEKVTDVKCEKCGNNMVVKHGRFGDFLACP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECC
GYPECKNTKPIVHQLDVKCPKCHGEIVLKKSKKGRKFYGCSNYPKCDFVSWFEPVERKCD
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VCGNYMVKRYTKAKGDYIECSNKECKHKIMPEKKQE
HHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MIMGQKLIIVESPAKAKTIGKYLDKTYHVEASMGHIRDLPKSQLGVDIDNNYEPKYITIR
CCCCCEEEEEECCHHHHHHHHHHCHHEEEECHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGELLTKIKKHAKKCDEIYLATDPDREGEAISWHLSQALKLEDNKKCRIVFNEITKSAV
CCHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEHHHHHCCCCCCCEEEEHHHHHHHHH
KNAIKEPREIDIDLVNAQQARRVLDRLVGYKISPILWRKVRWGLSAGRVQSVVLKMICDR
HHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
EKEIEEFIPKEYWTIECKLNKDRKDFLVKLISKDNKKIEINTEEESNNIIKELKQGQFTV
HHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCH
DKIKKTNKNKNPLPPFITSTLQQDAYRKLNFSTKKTMSVAQQLYEGIDIKGHGTVGLITY
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSVRISKEAQNKARQFLENVYGKEYIPEKPRDYKGKKNIQDAHEAIRPTNIEITPKI
EECCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEHHH
ALENLNSDQYKLYSLIWNRFMASQMAACVMEVNSVDVKNSEYIFRATGSIVKFDGFMIIY
HEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCEEEE
EYLNEDEKENINIPKLEKGELLKEKSIDSKQHFTQPPGRFSEASLVKVLEDNGIGRPSTY
HHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCH
APIISTLLERTYIERENRTLKSTELGVIVNNIMVEYFKPVVDAEFTAEMENKLDSIEEGN
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
ENWKNTVDEFYSPLKELIETAEKEVEKVTLEEKVTDVKCEKCGNNMVVKHGRFGDFLACP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECC
GYPECKNTKPIVHQLDVKCPKCHGEIVLKKSKKGRKFYGCSNYPKCDFVSWFEPVERKCD
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VCGNYMVKRYTKAKGDYIECSNKECKHKIMPEKKQE
HHHHHHHHHHHHCCCCEEECCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]