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The map label for this gene is rlmN

Identifier: 28210911

GI number: 28210911

Start: 1310567

End: 1311616

Strand: Direct

Name: rlmN

Synonym: CTC01223

Alternate gene names: 28210911

Gene position: 1310567-1311616 (Clockwise)

Preceding gene: 28210910

Following gene: 28210912

Centisome position: 46.82

GC content: 30.0

Gene sequence:

>1050_bases
GTGTTTAATATGATAAATTTATTAGACTTAAGCTTAGAGGATTTAAAAAAGTGGATGATAGAAAATAAAGAAAAAGAATT
TAGAGCAAAACAAGTATTAGACTGGGTATATAAAGGAGTTTATAATTTTGAAGCTATGAAAAATATACCTAAAGTTATTA
CAAATAAGCTTCAAGAAAATTTTTATCTTAGTGTTCCATCTGTAGTACAAAAATATGTATCTAAAGATGAAAGTACTGTT
AAATTTCTTTTTAAATATAATGATGGAAATATAATAGAATCTGTGGTTATGAAGTATAAACATGGCAACACCATTTGTGT
ATCTACCCAAGTAGGATGTAAAATGGGATGTACTTTTTGTGCTTCTACCATAGGTGGAATTGTAAGAAGTTTATCTCATG
GAGAAATTCTAGGGCAAGTATTAAAGGCACAGGAAGAAACAGGGGAAAGAATATCTAACATAGTTATGATGGGTAGTGGG
GAACCACTAGACAATTATGACAATAGTTTAAACTTCATAAGAATGGTAAATGCTGAAAATGGTCTCAATATAGGGCAAAG
ACATATAACACTTTCAACTTGTGGTATAGTTCCTAAAATAAGGCAGCTAGCAGAAGAAAATTTACAAATAACTTTAGCAA
TATCATTACATGCACCTAATGATAATATAAGAAGAAAAACCATGCCTATTGCTTCCGTTTATTCTGTAGAAGAGCTAATA
GAGGCATGCAACTACTATATAAACAAAACAAATAGAAGAATAACTTTTGAATACGCCTTAGTTAGTAATTTAAATGATAA
GGAGGTACATGCAGAAGAGTTAGCTACTCTATTAAAGGGAATGCTTTGTCATGTGAATTTAATTCCAGTTAACAAAATAG
ATGAAAAAGATTTTAAAAGTTCTTCCACCAATAGGATTAAAAACTTTAGTAATATATTACTAAAAAGTGGCATACAAACT
ACTATAAGAAGAGAAATGGGCTCGGACATAAATGCAGCCTGTGGACAGCTTCGAAGAAGATATGTTAAAAATAATAGTAA
AGAGGTGTAA

Upstream 100 bases:

>100_bases
TTTAGACAATTTTATATATGATGAATATGGTACACTTACCATATTACCAAATGAATATATGGATGGATTTTATATAGCCA
AATTTAAAAAACAGCGGTAG

Downstream 100 bases:

>100_bases
ATCTATGGTGGGTATGATAACAGATGTTGGGAATTTTAGAAAGATAAATGAAGATTATGTTGGATACTATATAGATGAGA
CAAAAGAAATATATATAGTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MFNMINLLDLSLEDLKKWMIENKEKEFRAKQVLDWVYKGVYNFEAMKNIPKVITNKLQENFYLSVPSVVQKYVSKDESTV
KFLFKYNDGNIIESVVMKYKHGNTICVSTQVGCKMGCTFCASTIGGIVRSLSHGEILGQVLKAQEETGERISNIVMMGSG
EPLDNYDNSLNFIRMVNAENGLNIGQRHITLSTCGIVPKIRQLAEENLQITLAISLHAPNDNIRRKTMPIASVYSVEELI
EACNYYINKTNRRITFEYALVSNLNDKEVHAEELATLLKGMLCHVNLIPVNKIDEKDFKSSSTNRIKNFSNILLKSGIQT
TIRREMGSDINAACGQLRRRYVKNNSKEV

Sequences:

>Translated_349_residues
MFNMINLLDLSLEDLKKWMIENKEKEFRAKQVLDWVYKGVYNFEAMKNIPKVITNKLQENFYLSVPSVVQKYVSKDESTV
KFLFKYNDGNIIESVVMKYKHGNTICVSTQVGCKMGCTFCASTIGGIVRSLSHGEILGQVLKAQEETGERISNIVMMGSG
EPLDNYDNSLNFIRMVNAENGLNIGQRHITLSTCGIVPKIRQLAEENLQITLAISLHAPNDNIRRKTMPIASVYSVEELI
EACNYYINKTNRRITFEYALVSNLNDKEVHAEELATLLKGMLCHVNLIPVNKIDEKDFKSSSTNRIKNFSNILLKSGIQT
TIRREMGSDINAACGQLRRRYVKNNSKEV
>Mature_349_residues
MFNMINLLDLSLEDLKKWMIENKEKEFRAKQVLDWVYKGVYNFEAMKNIPKVITNKLQENFYLSVPSVVQKYVSKDESTV
KFLFKYNDGNIIESVVMKYKHGNTICVSTQVGCKMGCTFCASTIGGIVRSLSHGEILGQVLKAQEETGERISNIVMMGSG
EPLDNYDNSLNFIRMVNAENGLNIGQRHITLSTCGIVPKIRQLAEENLQITLAISLHAPNDNIRRKTMPIASVYSVEELI
EACNYYINKTNRRITFEYALVSNLNDKEVHAEELATLLKGMLCHVNLIPVNKIDEKDFKSSSTNRIKNFSNILLKSGIQT
TIRREMGSDINAACGQLRRRYVKNNSKEV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=39.8255813953488, Blast_Score=248, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CLOTE (Q895P8)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781855.1
- ProteinModelPortal:   Q895P8
- GeneID:   1059303
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01223
- NMPDR:   fig|212717.1.peg.1077
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q895P8
- ProtClustDB:   PRK14454
- BioCyc:   CTET212717:CTC_01223-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39638; Mature: 39638

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNMINLLDLSLEDLKKWMIENKEKEFRAKQVLDWVYKGVYNFEAMKNIPKVITNKLQEN
CCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FYLSVPSVVQKYVSKDESTVKFLFKYNDGNIIESVVMKYKHGNTICVSTQVGCKMGCTFC
EEEEHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
ASTIGGIVRSLSHGEILGQVLKAQEETGERISNIVMMGSGEPLDNYDNSLNFIRMVNAEN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCC
GLNIGQRHITLSTCGIVPKIRQLAEENLQITLAISLHAPNDNIRRKTMPIASVYSVEELI
CCCCCCEEEEEHHHCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHH
EACNYYINKTNRRITFEYALVSNLNDKEVHAEELATLLKGMLCHVNLIPVNKIDEKDFKS
HHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHCC
SSTNRIKNFSNILLKSGIQTTIRREMGSDINAACGQLRRRYVKNNSKEV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFNMINLLDLSLEDLKKWMIENKEKEFRAKQVLDWVYKGVYNFEAMKNIPKVITNKLQEN
CCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FYLSVPSVVQKYVSKDESTVKFLFKYNDGNIIESVVMKYKHGNTICVSTQVGCKMGCTFC
EEEEHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
ASTIGGIVRSLSHGEILGQVLKAQEETGERISNIVMMGSGEPLDNYDNSLNFIRMVNAEN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEECCCC
GLNIGQRHITLSTCGIVPKIRQLAEENLQITLAISLHAPNDNIRRKTMPIASVYSVEELI
CCCCCCEEEEEHHHCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHH
EACNYYINKTNRRITFEYALVSNLNDKEVHAEELATLLKGMLCHVNLIPVNKIDEKDFKS
HHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHCC
SSTNRIKNFSNILLKSGIQTTIRREMGSDINAACGQLRRRYVKNNSKEV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129