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The map label for this gene is pilT [H]

Identifier: 28210816

GI number: 28210816

Start: 1186168

End: 1187223

Strand: Direct

Name: pilT [H]

Synonym: CTC01119

Alternate gene names: 28210816

Gene position: 1186168-1187223 (Clockwise)

Preceding gene: 28210815

Following gene: 28210817

Centisome position: 42.37

GC content: 31.44

Gene sequence:

>1056_bases
GTGGAAAGTTTAAGCAATTTGTTAGAAACAACATTACAACATAATGGTTCAGATTTACATCTTACAGTAGGTGTAGCTCC
AACAATTAGAGTAAATGGGGAGCTAACTCAAATATCTAAAGATAAATTAAAACCTAAAGATTTAGAGAAATATGCAAAGG
AGATTCTTGGAGAAGATTTTGAAGAATATTGTGAAAGAGGAGAAATAGACACTTCTTTTTCTATACCAGGATTAGGAAGA
TTCAGAGTTAATGTATTTAAACAAAGGGGTAGCCATGCTATTGCTATAAGAATTGTAGGGGTAAGGGTGCCGAGTTTAAA
TACTCTAAATTTCCCAGAAACCATAAAAGAGTTATCACTTATCAAAAGAGGACTTATATTAGTAACAGGACCTGCAGGAG
CAGGTAAAAGTACTACTTTAGCAGCCATGATAAATGAAATTAATTCAAAAAGATCAGCACATATAATAACTTTAGAAGAT
CCAATAGAATATCTTCATAAACATAATAATTCTATTGTAAACCAAAGAGAAATAGGAAAGGATACCAAAAACTATACTCA
AGCTCTTAGAGCAGTTTTAAGAGAAGATCCAGATGTTATATTAGTTGGAGAGATGAGGGATCTAGAAACTATATCAATAG
CTTTAACAGCTGCAGAAACAGGTCACTTAGTATTATCTAGTTTACATACTATTGGAGCAGCTAAAACTATAGATAGAATA
ATAGATGTTTTCCATTCATCACAACAACAACAAATAAGAATACAGTTAGCCACTGTCTTAAAGGGTATAGTATCTCAACA
GTTAGTTCCTAGAAAAGATGGAGAAGGTAGAGTAGCAGCCTTAGAAATTATGATTGCTACTCATGGAATACAAAATATGA
TAAGAGAAGGTAAGGTTCATCAAATAGAATCTTCTATGCAAACAGGAATTAAATATGGAATGAAAACTATGGATATGGCT
TTAGCAGAATTATATAAAAAAGAATTGATATCTTATGATAAGGCAATAGCCTACTCTACAGATAGGGAAATTATTAAAAA
AATGATATCCATATAA

Upstream 100 bases:

>100_bases
TATAATGGCAATTAATGGAGAAGATATGTTAAGACTCCAGGCTTATAAGGCTATTGATATATGGGGAATATATATATATT
AAACGGGGAGGATAAATAAA

Downstream 100 bases:

>100_bases
AAAATAGGAGTATTAATACTCCTATTTTTTATATATGTAATAAATGGTGTATAATATATATAGGATAAATTCATAAAAAT
TCATCAGAATGCAAATAATT

Product: pili retraction protein pilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MESLSNLLETTLQHNGSDLHLTVGVAPTIRVNGELTQISKDKLKPKDLEKYAKEILGEDFEEYCERGEIDTSFSIPGLGR
FRVNVFKQRGSHAIAIRIVGVRVPSLNTLNFPETIKELSLIKRGLILVTGPAGAGKSTTLAAMINEINSKRSAHIITLED
PIEYLHKHNNSIVNQREIGKDTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
IDVFHSSQQQQIRIQLATVLKGIVSQQLVPRKDGEGRVAALEIMIATHGIQNMIREGKVHQIESSMQTGIKYGMKTMDMA
LAELYKKELISYDKAIAYSTDREIIKKMISI

Sequences:

>Translated_351_residues
MESLSNLLETTLQHNGSDLHLTVGVAPTIRVNGELTQISKDKLKPKDLEKYAKEILGEDFEEYCERGEIDTSFSIPGLGR
FRVNVFKQRGSHAIAIRIVGVRVPSLNTLNFPETIKELSLIKRGLILVTGPAGAGKSTTLAAMINEINSKRSAHIITLED
PIEYLHKHNNSIVNQREIGKDTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
IDVFHSSQQQQIRIQLATVLKGIVSQQLVPRKDGEGRVAALEIMIATHGIQNMIREGKVHQIESSMQTGIKYGMKTMDMA
LAELYKKELISYDKAIAYSTDREIIKKMISI
>Mature_351_residues
MESLSNLLETTLQHNGSDLHLTVGVAPTIRVNGELTQISKDKLKPKDLEKYAKEILGEDFEEYCERGEIDTSFSIPGLGR
FRVNVFKQRGSHAIAIRIVGVRVPSLNTLNFPETIKELSLIKRGLILVTGPAGAGKSTTLAAMINEINSKRSAHIITLED
PIEYLHKHNNSIVNQREIGKDTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
IDVFHSSQQQQIRIQLATVLKGIVSQQLVPRKDGEGRVAALEIMIATHGIQNMIREGKVHQIESSMQTGIKYGMKTMDMA
LAELYKKELISYDKAIAYSTDREIIKKMISI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=316, Percent_Identity=43.3544303797468, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1789723, Length=178, Percent_Identity=41.5730337078652, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786296, Length=295, Percent_Identity=31.5254237288136, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38971; Mature: 38971

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLSNLLETTLQHNGSDLHLTVGVAPTIRVNGELTQISKDKLKPKDLEKYAKEILGEDF
CCHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCEEHHHHHCCCHHHHHHHHHHHHCCHH
EEYCERGEIDTSFSIPGLGRFRVNVFKQRGSHAIAIRIVGVRVPSLNTLNFPETIKELSL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
IKRGLILVTGPAGAGKSTTLAAMINEINSKRSAHIITLEDPIEYLHKHNNSIVNQREIGK
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCHHHCCC
DTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
CHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFHSSQQQQIRIQLATVLKGIVSQQLVPRKDGEGRVAALEIMIATHGIQNMIREGKVH
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHH
QIESSMQTGIKYGMKTMDMALAELYKKELISYDKAIAYSTDREIIKKMISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCC
>Mature Secondary Structure
MESLSNLLETTLQHNGSDLHLTVGVAPTIRVNGELTQISKDKLKPKDLEKYAKEILGEDF
CCHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCEEHHHHHCCCHHHHHHHHHHHHCCHH
EEYCERGEIDTSFSIPGLGRFRVNVFKQRGSHAIAIRIVGVRVPSLNTLNFPETIKELSL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
IKRGLILVTGPAGAGKSTTLAAMINEINSKRSAHIITLEDPIEYLHKHNNSIVNQREIGK
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCHHHCCC
DTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
CHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFHSSQQQQIRIQLATVLKGIVSQQLVPRKDGEGRVAALEIMIATHGIQNMIREGKVH
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHH
QIESSMQTGIKYGMKTMDMALAELYKKELISYDKAIAYSTDREIIKKMISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]