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The map label for this gene is polC [H]

Identifier: 28210814

GI number: 28210814

Start: 1183756

End: 1184898

Strand: Direct

Name: polC [H]

Synonym: CTC01116

Alternate gene names: 28210814

Gene position: 1183756-1184898 (Clockwise)

Preceding gene: 28210813

Following gene: 28210815

Centisome position: 42.29

GC content: 24.23

Gene sequence:

>1143_bases
ATGTTTATTTATGCGTTTAAAATAAGTACTATTAAGAAAAATATAAAAGATCAATCTTCAAATAATGAAGTAGTTAATGA
AAAAACAGAAGATAATCTCTTAAAGCAAACCGATTATTTAAGTAATATTCACGAATTAGAAGATGATAAAGGGAGAGAAG
AAACATCAGACTTGTCTAGCGATGAATATTATAAAAAACTTTGTGGAAGAGACAAACATATTAATTATTCTAAAAGTGTT
TTTGATGAATTTGTTGTAATAGATTTAGAAACTACTGGGCTATATCCAGTTACAGATAAAATTATAGAAATTACGGCAAT
TAAATATAAAAGTGGACAAATAGTAGAAAAGTATAATACTTTGATTAATCCTAAAATCAATATACCTAAAAGGGCTACGG
AAATTAATAATATAACTAATAATATGGTTAAAGATAGCCCTGTAATAGAAGATGTCTTACCAGAGTTATTAAAATTTATA
AAAGAGTACCCATTAGTTGCACATAACGCTAGCTTTGATATAAAATTTTTGAATGCAAATTTAGCTTTAATAAATAGAGA
AATAAAAAATGTTGCAATTGATACCCTTCAATTAAGTAGAGCTATGTATACATTTTTACCGAATCATAAATTAACCACGA
TTAAAGAACATTTATGTATATCGGAAGACAATTCACATAGAGCTTTACCAGACTGTATTACAACTGCTGAAATATATTTA
GATTATTGCTATAAAGCAAATAATAATTTAAAAGAATTTAATGAATTAGAAAAAGCATGTTTTAAGGTAATAAAAGATAT
GTTAAATAAAAATAATAGAGATACTGAATTTTTAAAACTAAAACACACAGGGAATTATACAGATATAGCATATTTTTATC
CATTAGTTAGAATAAAAGTTGGTGATAGAAAACAATATTTTCTTACTAACATTCCACTTGAAGAATTAAAAGAACATGAA
ACAGAAAAACCTTCTAAATCTGAAAATTTTAAATCAAGAATACATTTAAAATCAGAAGAGGATTTAAAGCCATTTGAGGA
ATATATTATACAAATCTTTGATGAAAAAAAGAAAAGTTTTGAATGGTATAAAAACAACATTAAAAGTTCAGAAATTGAAA
TAGTTAAATATCTTGCAAATTAA

Upstream 100 bases:

>100_bases
TAAAAAATATTTTATTTGTTTAATAACTTTTATTTTAACATTTGTAGTCGGATTAAAATCAGCTATATTTTTTTTAATAT
CATTAGGATTTTTAATAGCA

Downstream 100 bases:

>100_bases
TATAAAAGAAGATCTAAAGTTTAAGCCTTAGATCTTCTCTTATTTTATGTCAAAAATAAGATGTATTAAAAATCCCTACT
ATTATATAATATATGTAATA

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MFIYAFKISTIKKNIKDQSSNNEVVNEKTEDNLLKQTDYLSNIHELEDDKGREETSDLSSDEYYKKLCGRDKHINYSKSV
FDEFVVIDLETTGLYPVTDKIIEITAIKYKSGQIVEKYNTLINPKINIPKRATEINNITNNMVKDSPVIEDVLPELLKFI
KEYPLVAHNASFDIKFLNANLALINREIKNVAIDTLQLSRAMYTFLPNHKLTTIKEHLCISEDNSHRALPDCITTAEIYL
DYCYKANNNLKEFNELEKACFKVIKDMLNKNNRDTEFLKLKHTGNYTDIAYFYPLVRIKVGDRKQYFLTNIPLEELKEHE
TEKPSKSENFKSRIHLKSEEDLKPFEEYIIQIFDEKKKSFEWYKNNIKSSEIEIVKYLAN

Sequences:

>Translated_380_residues
MFIYAFKISTIKKNIKDQSSNNEVVNEKTEDNLLKQTDYLSNIHELEDDKGREETSDLSSDEYYKKLCGRDKHINYSKSV
FDEFVVIDLETTGLYPVTDKIIEITAIKYKSGQIVEKYNTLINPKINIPKRATEINNITNNMVKDSPVIEDVLPELLKFI
KEYPLVAHNASFDIKFLNANLALINREIKNVAIDTLQLSRAMYTFLPNHKLTTIKEHLCISEDNSHRALPDCITTAEIYL
DYCYKANNNLKEFNELEKACFKVIKDMLNKNNRDTEFLKLKHTGNYTDIAYFYPLVRIKVGDRKQYFLTNIPLEELKEHE
TEKPSKSENFKSRIHLKSEEDLKPFEEYIIQIFDEKKKSFEWYKNNIKSSEIEIVKYLAN
>Mature_380_residues
MFIYAFKISTIKKNIKDQSSNNEVVNEKTEDNLLKQTDYLSNIHELEDDKGREETSDLSSDEYYKKLCGRDKHINYSKSV
FDEFVVIDLETTGLYPVTDKIIEITAIKYKSGQIVEKYNTLINPKINIPKRATEINNITNNMVKDSPVIEDVLPELLKFI
KEYPLVAHNASFDIKFLNANLALINREIKNVAIDTLQLSRAMYTFLPNHKLTTIKEHLCISEDNSHRALPDCITTAEIYL
DYCYKANNNLKEFNELEKACFKVIKDMLNKNNRDTEFLKLKHTGNYTDIAYFYPLVRIKVGDRKQYFLTNIPLEELKEHE
TEKPSKSENFKSRIHLKSEEDLKPFEEYIIQIFDEKKKSFEWYKNNIKSSEIEIVKYLAN

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788149, Length=160, Percent_Identity=33.75, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786409, Length=173, Percent_Identity=28.9017341040462, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44580; Mature: 44580

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIYAFKISTIKKNIKDQSSNNEVVNEKTEDNLLKQTDYLSNIHELEDDKGREETSDLSS
CEEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
DEYYKKLCGRDKHINYSKSVFDEFVVIDLETTGLYPVTDKIIEITAIKYKSGQIVEKYNT
HHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHEEEEEEEEECCCCHHHHHHH
LINPKINIPKRATEINNITNNMVKDSPVIEDVLPELLKFIKEYPLVAHNASFDIKFLNAN
HCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCC
LALINREIKNVAIDTLQLSRAMYTFLPNHKLTTIKEHLCISEDNSHRALPDCITTAEIYL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DYCYKANNNLKEFNELEKACFKVIKDMLNKNNRDTEFLKLKHTGNYTDIAYFYPLVRIKV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHEEEEEEEEEE
GDRKQYFLTNIPLEELKEHETEKPSKSENFKSRIHLKSEEDLKPFEEYIIQIFDEKKKSF
CCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHH
EWYKNNIKSSEIEIVKYLAN
HHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MFIYAFKISTIKKNIKDQSSNNEVVNEKTEDNLLKQTDYLSNIHELEDDKGREETSDLSS
CEEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
DEYYKKLCGRDKHINYSKSVFDEFVVIDLETTGLYPVTDKIIEITAIKYKSGQIVEKYNT
HHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHEEEEEEEEECCCCHHHHHHH
LINPKINIPKRATEINNITNNMVKDSPVIEDVLPELLKFIKEYPLVAHNASFDIKFLNAN
HCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCC
LALINREIKNVAIDTLQLSRAMYTFLPNHKLTTIKEHLCISEDNSHRALPDCITTAEIYL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DYCYKANNNLKEFNELEKACFKVIKDMLNKNNRDTEFLKLKHTGNYTDIAYFYPLVRIKV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHEEEEEEEEEE
GDRKQYFLTNIPLEELKEHETEKPSKSENFKSRIHLKSEEDLKPFEEYIIQIFDEKKKSF
CCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHH
EWYKNNIKSSEIEIVKYLAN
HHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]