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The map label for this gene is rnjA [H]

Identifier: 28210760

GI number: 28210760

Start: 1128164

End: 1129831

Strand: Direct

Name: rnjA [H]

Synonym: CTC01058

Alternate gene names: 28210760

Gene position: 1128164-1129831 (Clockwise)

Preceding gene: 28210759

Following gene: 28210761

Centisome position: 40.3

GC content: 33.27

Gene sequence:

>1668_bases
ATGAAGAAAGAAAGAGACAAAGTAAAAGTCATACCCTTAGGTGGCTTAAATGAAATCGGGAAAAATATTACAGCTTTTGA
ATATAAAAATGATATAGTTGTAATAGACTGCGGTTTGAAATTTCCTGATGAAGAGATGTTAGGTATAGATATAGTAATAC
CGGATATAAGCTATTTATTGAAAAATAAAGAAAGGGTAAAAGGAATATTTTTAACCCATGGACATGAAGATCATATAGGA
GCTCTTCCATATGTTCTAAAAGAATTAAATGTTCCAGTATATGGTACTAAATTAACTCTTGGAATTCTTGAAACAAAACT
AAAAGAGCATAATTTATTAAGCTCAGTAAAATTAAACTGTATTAAGCCTAGGGACATGATAAAACTAGAAAATTGCACGG
TGGAGTTTATAAGAACAAGTCATAGTATTGCGGATTCAGTAGCTATAGCAATACATACTCCAGTGGGAGCAATACTTCAT
ACAGGAGACTTTAAAATAGACTACACTCCTATAGACGGACATGTAGCGGATTTATCTAGATTTGCAGAATTAGGCAGAAA
AGGTGTTGCCTTAATGCTAGCAGATAGCACCAATGTAGAAAGACCTGGGTATACCATGTCAGAAAGCACTGTAGGAAAAA
CCTTTGATAATATATTTACAAAGGGAAAAGGTAGAATAATAGTAGCTACTTTTGCATCAAATATACATAGAGTGCAGCAA
ATAATAAGTACTGCAGTAAAATTTGATAGAAAAGTTGCTTTCTCTGGAAGAAGTATGGAAAATATAGTAGAGGTAGCAAA
GGAATTAGGTTATATAAAAGCACCTTTAGAAACCTTTATAGACATTGATTCTATAAATAAATATCCAGATAATAAGGTAG
TAATAATAACTACAGGGAGTCAGGGAGAGCCTATGTCAGCGCTTTCAAGAATGGCGGCATCAGAGCATAGAAAAGTAAAT
ATAGTACCTGGAGATACCGTGGTTATATCAGCAACACCTATACCAGGTAATGAAAAACTAGTTTCAAGAGTTATAAATCA
ATTATTTCAAAAAGGAGCAGATGTTATATATGAGGCTTTAGCAGATATCCACGTTTCAGGACACGCGTGTCAAGAAGAAC
TAAAGCTTATGCATACTTTAGTAAGACCTAAGTATTTTATTCCAGTACATGGAGAATATAGACATCTAAAACAACATGGA
GAATTAGCAGAAAAATTAGGTATGCGCCATGAAAATATCGTTATAGCAGGAAATGGAGATGTTATAGAGGTAGCTAGAGA
TTGTATTAAGAAAAATGGTACAGTAATGTCAGGTCAAGTTTTTGTAGATGGATTAGGAGTGGGAGATGTAGGAAATATCG
TATTAAGAGATAGAAGGCACCTTTCACAGGATGGTATTTTAACAGTAGTGGTAACCATTGAAAGAGGAACAGGAATGGTT
ATAGCAGGTCCAGATATAATCTCTAGAGGATTTGTATATGTAAGAGAATCTGAATACTTAATGGGTGAGGCAAGAGAAGT
TGTAAAAGAAGTACTAAGAAAGTGTGAAGAAAATCATATTGTAGAGTGGTCTACTATAAAGTCAAATATAAAGGATGCAC
TTAGGGCATTTTTATATGAAAAAACTAAGAGAAGACCTATGATACTTCCTATTATAATGGAAATTTAA

Upstream 100 bases:

>100_bases
CATACCGTTAAATTTTATGGAATATGTAAGGATTGTTTAAAAAAGTCAAAGTAATAGATTTATAAATATATAATCTTAAA
TTTGAAAAAGGAGGGAAAAA

Downstream 100 bases:

>100_bases
TTTTTTTAGCATTGAGTTAAAATTAAAAAATTTTAACTCAATGCTAAAAATTAATATATAATTGTGTTATAGGAGGAATC
TTTTATGAATTTATATGATA

Product: Zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKKERDKVKVIPLGGLNEIGKNITAFEYKNDIVVIDCGLKFPDEEMLGIDIVIPDISYLLKNKERVKGIFLTHGHEDHIG
ALPYVLKELNVPVYGTKLTLGILETKLKEHNLLSSVKLNCIKPRDMIKLENCTVEFIRTSHSIADSVAIAIHTPVGAILH
TGDFKIDYTPIDGHVADLSRFAELGRKGVALMLADSTNVERPGYTMSESTVGKTFDNIFTKGKGRIIVATFASNIHRVQQ
IISTAVKFDRKVAFSGRSMENIVEVAKELGYIKAPLETFIDIDSINKYPDNKVVIITTGSQGEPMSALSRMAASEHRKVN
IVPGDTVVISATPIPGNEKLVSRVINQLFQKGADVIYEALADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHG
ELAEKLGMRHENIVIAGNGDVIEVARDCIKKNGTVMSGQVFVDGLGVGDVGNIVLRDRRHLSQDGILTVVVTIERGTGMV
IAGPDIISRGFVYVRESEYLMGEAREVVKEVLRKCEENHIVEWSTIKSNIKDALRAFLYEKTKRRPMILPIIMEI

Sequences:

>Translated_555_residues
MKKERDKVKVIPLGGLNEIGKNITAFEYKNDIVVIDCGLKFPDEEMLGIDIVIPDISYLLKNKERVKGIFLTHGHEDHIG
ALPYVLKELNVPVYGTKLTLGILETKLKEHNLLSSVKLNCIKPRDMIKLENCTVEFIRTSHSIADSVAIAIHTPVGAILH
TGDFKIDYTPIDGHVADLSRFAELGRKGVALMLADSTNVERPGYTMSESTVGKTFDNIFTKGKGRIIVATFASNIHRVQQ
IISTAVKFDRKVAFSGRSMENIVEVAKELGYIKAPLETFIDIDSINKYPDNKVVIITTGSQGEPMSALSRMAASEHRKVN
IVPGDTVVISATPIPGNEKLVSRVINQLFQKGADVIYEALADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHG
ELAEKLGMRHENIVIAGNGDVIEVARDCIKKNGTVMSGQVFVDGLGVGDVGNIVLRDRRHLSQDGILTVVVTIERGTGMV
IAGPDIISRGFVYVRESEYLMGEAREVVKEVLRKCEENHIVEWSTIKSNIKDALRAFLYEKTKRRPMILPIIMEI
>Mature_555_residues
MKKERDKVKVIPLGGLNEIGKNITAFEYKNDIVVIDCGLKFPDEEMLGIDIVIPDISYLLKNKERVKGIFLTHGHEDHIG
ALPYVLKELNVPVYGTKLTLGILETKLKEHNLLSSVKLNCIKPRDMIKLENCTVEFIRTSHSIADSVAIAIHTPVGAILH
TGDFKIDYTPIDGHVADLSRFAELGRKGVALMLADSTNVERPGYTMSESTVGKTFDNIFTKGKGRIIVATFASNIHRVQQ
IISTAVKFDRKVAFSGRSMENIVEVAKELGYIKAPLETFIDIDSINKYPDNKVVIITTGSQGEPMSALSRMAASEHRKVN
IVPGDTVVISATPIPGNEKLVSRVINQLFQKGADVIYEALADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHG
ELAEKLGMRHENIVIAGNGDVIEVARDCIKKNGTVMSGQVFVDGLGVGDVGNIVLRDRRHLSQDGILTVVVTIERGTGMV
IAGPDIISRGFVYVRESEYLMGEAREVVKEVLRKCEENHIVEWSTIKSNIKDALRAFLYEKTKRRPMILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61671; Mature: 61671

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKERDKVKVIPLGGLNEIGKNITAFEYKNDIVVIDCGLKFPDEEMLGIDIVIPDISYLL
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
KNKERVKGIFLTHGHEDHIGALPYVLKELNVPVYGTKLTLGILETKLKEHNLLSSVKLNC
CCHHHHCEEEEECCCCCCCCHHHHHHHHCCCCEEECEEEHHHHHHHHHHHHHHHHHEEEE
IKPRDMIKLENCTVEFIRTSHSIADSVAIAIHTPVGAILHTGDFKIDYTPIDGHVADLSR
ECCCCCEEECCCEEEEHHHHHHHHCCEEEEEECCCCEEEECCCEEEEEEECCCHHHHHHH
FAELGRKGVALMLADSTNVERPGYTMSESTVGKTFDNIFTKGKGRIIVATFASNIHRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
IISTAVKFDRKVAFSGRSMENIVEVAKELGYIKAPLETFIDIDSINKYPDNKVVIITTGS
HHHHHHHHCCEEEECCCCHHHHHHHHHHCCCHHCCHHHHEEHHCCCCCCCCEEEEEEECC
QGEPMSALSRMAASEHRKVNIVPGDTVVISATPIPGNEKLVSRVINQLFQKGADVIYEAL
CCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHGELAEKLGMRHENIVIAGNGD
HHHCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VIEVARDCIKKNGTVMSGQVFVDGLGVGDVGNIVLRDRRHLSQDGILTVVVTIERGTGMV
HHHHHHHHHHCCCCEEECEEEEEECCCCCCCCCEECCCHHCCCCCEEEEEEEEECCCEEE
IAGPDIISRGFVYVRESEYLMGEAREVVKEVLRKCEENHIVEWSTIKSNIKDALRAFLYE
EECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEI
HHCCCCEEEEEEECC
>Mature Secondary Structure
MKKERDKVKVIPLGGLNEIGKNITAFEYKNDIVVIDCGLKFPDEEMLGIDIVIPDISYLL
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
KNKERVKGIFLTHGHEDHIGALPYVLKELNVPVYGTKLTLGILETKLKEHNLLSSVKLNC
CCHHHHCEEEEECCCCCCCCHHHHHHHHCCCCEEECEEEHHHHHHHHHHHHHHHHHEEEE
IKPRDMIKLENCTVEFIRTSHSIADSVAIAIHTPVGAILHTGDFKIDYTPIDGHVADLSR
ECCCCCEEECCCEEEEHHHHHHHHCCEEEEEECCCCEEEECCCEEEEEEECCCHHHHHHH
FAELGRKGVALMLADSTNVERPGYTMSESTVGKTFDNIFTKGKGRIIVATFASNIHRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
IISTAVKFDRKVAFSGRSMENIVEVAKELGYIKAPLETFIDIDSINKYPDNKVVIITTGS
HHHHHHHHCCEEEECCCCHHHHHHHHHHCCCHHCCHHHHEEHHCCCCCCCCEEEEEEECC
QGEPMSALSRMAASEHRKVNIVPGDTVVISATPIPGNEKLVSRVINQLFQKGADVIYEAL
CCCHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADIHVSGHACQEELKLMHTLVRPKYFIPVHGEYRHLKQHGELAEKLGMRHENIVIAGNGD
HHHCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VIEVARDCIKKNGTVMSGQVFVDGLGVGDVGNIVLRDRRHLSQDGILTVVVTIERGTGMV
HHHHHHHHHHCCCCEEECEEEEEECCCCCCCCCEECCCHHCCCCCEEEEEEEEECCCEEE
IAGPDIISRGFVYVRESEYLMGEAREVVKEVLRKCEENHIVEWSTIKSNIKDALRAFLYE
EECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEI
HHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]