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The map label for this gene is rnfA [H]

Identifier: 28210728

GI number: 28210728

Start: 1091410

End: 1091985

Strand: Direct

Name: rnfA [H]

Synonym: CTC01023

Alternate gene names: 28210728

Gene position: 1091410-1091985 (Clockwise)

Preceding gene: 28210727

Following gene: 28210729

Centisome position: 38.99

GC content: 32.12

Gene sequence:

>576_bases
ATGAAGATATTTACCATATTTTTAAGTGCGCTATTAGTTAATAACTTCGTTTTATCTAGATTCTTAGGAATATGTCCTTT
CCTTGGTGTTTCCAAAAAAGTAGAAACTGCTACAGGAATGGGGGCAGCAGTTACGTTTGTTATGGCGTTAGCGTCTATTA
TGACTTATTTAGTTGAAAAATTTATACTAGTAAAATTTGAGATAACATATTTATCAACATTGGCATTTATACTTGTTATA
GCATCTCTAGTTCAATTTGTTGAGATGGTAATTAAAAAGGTAAGTCCTGATTTGTATAAAGCATTAGGTATATTCTTACC
TTTAATAACAACAAACTGTGCTGTTCTAGGAGTTGCAGTTATTAACAGTGATGAAGGATATACTTTAATTGAATCATTAG
TAAACTCTGTAGGAGCGGCACTAGGATTTACATTAGCCCTTGTTTTACTTGCAGGAATTAGAGAAAAGTTAGATACTAAC
GAATATATACCAGAAGCACTAAAAGGTCTACCAATAACACTTGTTACAGCAGGATTAATGGCTATAGCGTTTCTAGGATT
CCAAGGACTAATATAA

Upstream 100 bases:

>100_bases
ATATTATTGGCAATATTAAATGCTAGAAAATTAAAGAAAGCTAAAAACAATTAAAAATAAGTTGAAGCTTATTTTATAAG
AAAGGTGGATTTTAAAGGAT

Downstream 100 bases:

>100_bases
AGTAAAAGTTTAGGAGGTGTTAACGTGGATTTAAATAATTTAATAGCCCCTGTCTTAAGTTTAGGGGGACTTGGTATAAT
TTTCGGTGCTCTATTAGGAT

Product: rnfA/nqrE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKIFTIFLSALLVNNFVLSRFLGICPFLGVSKKVETATGMGAAVTFVMALASIMTYLVEKFILVKFEITYLSTLAFILVI
ASLVQFVEMVIKKVSPDLYKALGIFLPLITTNCAVLGVAVINSDEGYTLIESLVNSVGAALGFTLALVLLAGIREKLDTN
EYIPEALKGLPITLVTAGLMAIAFLGFQGLI

Sequences:

>Translated_191_residues
MKIFTIFLSALLVNNFVLSRFLGICPFLGVSKKVETATGMGAAVTFVMALASIMTYLVEKFILVKFEITYLSTLAFILVI
ASLVQFVEMVIKKVSPDLYKALGIFLPLITTNCAVLGVAVINSDEGYTLIESLVNSVGAALGFTLALVLLAGIREKLDTN
EYIPEALKGLPITLVTAGLMAIAFLGFQGLI
>Mature_191_residues
MKIFTIFLSALLVNNFVLSRFLGICPFLGVSKKVETATGMGAAVTFVMALASIMTYLVEKFILVKFEITYLSTLAFILVI
ASLVQFVEMVIKKVSPDLYKALGIFLPLITTNCAVLGVAVINSDEGYTLIESLVNSVGAALGFTLALVLLAGIREKLDTN
EYIPEALKGLPITLVTAGLMAIAFLGFQGLI

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=186, Percent_Identity=57.5268817204301, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI1787919, Length=155, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20492; Mature: 20492

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFTIFLSALLVNNFVLSRFLGICPFLGVSKKVETATGMGAAVTFVMALASIMTYLVEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FILVKFEITYLSTLAFILVIASLVQFVEMVIKKVSPDLYKALGIFLPLITTNCAVLGVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
INSDEGYTLIESLVNSVGAALGFTLALVLLAGIREKLDTNEYIPEALKGLPITLVTAGLM
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
AIAFLGFQGLI
HHHHHHHHCCC
>Mature Secondary Structure
MKIFTIFLSALLVNNFVLSRFLGICPFLGVSKKVETATGMGAAVTFVMALASIMTYLVEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FILVKFEITYLSTLAFILVIASLVQFVEMVIKKVSPDLYKALGIFLPLITTNCAVLGVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
INSDEGYTLIESLVNSVGAALGFTLALVLLAGIREKLDTNEYIPEALKGLPITLVTAGLM
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
AIAFLGFQGLI
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA