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The map label for this gene is tpl [H]

Identifier: 28210535

GI number: 28210535

Start: 865881

End: 867272

Strand: Direct

Name: tpl [H]

Synonym: CTC00818

Alternate gene names: 28210535

Gene position: 865881-867272 (Clockwise)

Preceding gene: 28210533

Following gene: 28210536

Centisome position: 30.93

GC content: 33.91

Gene sequence:

>1392_bases
ATGGATTATATGGATTTAAGTAAGTACCCTGCAGAACCTTTTAAAATTAAGAGTGTTGAGCCTGTTAAAATGATTTCAAG
AGAAGAACGTGAAATAGCTATGAAAGAGGCTGGATACAACACATTTAATTTAAGATCAGAGGATGTATATATTGATTTAC
TTACTGACTCAGGAACCAATGCTATGAGTGATGCTCAATGGGCAGGTATGATGATTGGAGACGAAGCTTATGCTGGAAGT
AAAAACTGGTTATTTTTAGAATCAACTATAAAAGAATTATTTGGATTTAAACATGTTGTTCCTACTCACCAAGGACGTGG
TGCAGAAAATCTCCTTTCAAGTATAGCTATTAAACCAGGGCAATATGTAGCAGGAAATATGTATTTTACAACTACTAGAT
ATCATCAAGAAAAAAATGGTGGTATTTTTGTAGATATAATAAGAGATGAAGCTCACGATGCAAGTATAGATATTCCTTTT
AAAGGTAATATTGATGTTAATAAATTAGAAAAACTTATTGAAGAAAAAGGTGCAGAAAACATAGCTTATGTATGCCTTGC
TGTTACTGTAAATCTAGCAGGTGGACAACCAGTTTCTATGGCTAATATGAGAGCAGTTAGAGAACTTACAGCAAAACATG
GAATTAAGGTATTCTATGATGCTACTAGATGTGTTGAAAATGCATATTTTATAAAAGAACAAGAGGAAGGATATGCAGAT
GTTTCAATTAAAGATATAGTACACGAAATGTTTAGCTATTCCGATGGTGCTACTATGTCTGGTAAAAAAGATGGTATTGT
AAATATCGGTGGATTCTTAGCAATTAATGATGAAGAGTTATTTGGAAAAGCTAAAGAAATAGTAGTAGTTTACGAAGGTA
TGCCATCCTATGGTGGTATGACTGGCCGTGATATGCAGGCTATAGCAATTGGATTTAGAGAAGCAATGCAATATGAATAT
ATAGAACACAGAATAAGACAGGTTAGATATCTTGGAGATAGATTAAAAGAAGCGGGAGTTCCTATAGTTGAACCAGTTGG
AGGACATGCTGTATTCTTAGATGCAAGAAGATTTTGCCCACATCTAGATCAAGAGCAATTCCCAGCTCAATCACTAGCTG
CTTCACTTTACATTGACTCTGGTGTTAGATCAATGGAACGTGGTATAGTTTCAGCAGGCAGAGATGTTAATACAGGCGAA
AATCATAAACCAAAATTAGAGACAGTTAGACTTACTATTCCAAGAAGAGTTTACACTTACAAACATATGGATGTTGTTGC
TGAATCAGTTATACATCTATATAAGCATAAAGAAGATATAAGACCACTTAAATTCACTTATGAACCTGCACAATTAAGAT
TCTTTACTGCAAAATTTGATTATGCAGACTAA

Upstream 100 bases:

>100_bases
TTATATTGACATTCCATATTTTTCATTATACAATTAAATTGGTCGGTGATGTATGATGCATCATGCAAATATATATTGAA
TTTCAAGGAGGAGTTTTGTT

Downstream 100 bases:

>100_bases
TTAGAATAAAAATTTATTATTCTATATTTCCAGGCACAATGCCTGGAAATATAGAATATATATTATAAAGAAATATAACT
GATCAGAAATAAGAATACTA

Product: tyrosine phenol-lyase

Products: NA

Alternate protein names: Beta-tyrosinase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDYMDLSKYPAEPFKIKSVEPVKMISREEREIAMKEAGYNTFNLRSEDVYIDLLTDSGTNAMSDAQWAGMMIGDEAYAGS
KNWLFLESTIKELFGFKHVVPTHQGRGAENLLSSIAIKPGQYVAGNMYFTTTRYHQEKNGGIFVDIIRDEAHDASIDIPF
KGNIDVNKLEKLIEEKGAENIAYVCLAVTVNLAGGQPVSMANMRAVRELTAKHGIKVFYDATRCVENAYFIKEQEEGYAD
VSIKDIVHEMFSYSDGATMSGKKDGIVNIGGFLAINDEELFGKAKEIVVVYEGMPSYGGMTGRDMQAIAIGFREAMQYEY
IEHRIRQVRYLGDRLKEAGVPIVEPVGGHAVFLDARRFCPHLDQEQFPAQSLAASLYIDSGVRSMERGIVSAGRDVNTGE
NHKPKLETVRLTIPRRVYTYKHMDVVAESVIHLYKHKEDIRPLKFTYEPAQLRFFTAKFDYAD

Sequences:

>Translated_463_residues
MDYMDLSKYPAEPFKIKSVEPVKMISREEREIAMKEAGYNTFNLRSEDVYIDLLTDSGTNAMSDAQWAGMMIGDEAYAGS
KNWLFLESTIKELFGFKHVVPTHQGRGAENLLSSIAIKPGQYVAGNMYFTTTRYHQEKNGGIFVDIIRDEAHDASIDIPF
KGNIDVNKLEKLIEEKGAENIAYVCLAVTVNLAGGQPVSMANMRAVRELTAKHGIKVFYDATRCVENAYFIKEQEEGYAD
VSIKDIVHEMFSYSDGATMSGKKDGIVNIGGFLAINDEELFGKAKEIVVVYEGMPSYGGMTGRDMQAIAIGFREAMQYEY
IEHRIRQVRYLGDRLKEAGVPIVEPVGGHAVFLDARRFCPHLDQEQFPAQSLAASLYIDSGVRSMERGIVSAGRDVNTGE
NHKPKLETVRLTIPRRVYTYKHMDVVAESVIHLYKHKEDIRPLKFTYEPAQLRFFTAKFDYAD
>Mature_463_residues
MDYMDLSKYPAEPFKIKSVEPVKMISREEREIAMKEAGYNTFNLRSEDVYIDLLTDSGTNAMSDAQWAGMMIGDEAYAGS
KNWLFLESTIKELFGFKHVVPTHQGRGAENLLSSIAIKPGQYVAGNMYFTTTRYHQEKNGGIFVDIIRDEAHDASIDIPF
KGNIDVNKLEKLIEEKGAENIAYVCLAVTVNLAGGQPVSMANMRAVRELTAKHGIKVFYDATRCVENAYFIKEQEEGYAD
VSIKDIVHEMFSYSDGATMSGKKDGIVNIGGFLAINDEELFGKAKEIVVVYEGMPSYGGMTGRDMQAIAIGFREAMQYEY
IEHRIRQVRYLGDRLKEAGVPIVEPVGGHAVFLDARRFCPHLDQEQFPAQSLAASLYIDSGVRSMERGIVSAGRDVNTGE
NHKPKLETVRLTIPRRVYTYKHMDVVAESVIHLYKHKEDIRPLKFTYEPAQLRFFTAKFDYAD

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=469, Percent_Identity=43.0703624733475, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176
- InterPro:   IPR013441 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.2 [H]

Molecular weight: Translated: 52170; Mature: 52170

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYMDLSKYPAEPFKIKSVEPVKMISREEREIAMKEAGYNTFNLRSEDVYIDLLTDSGTN
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCC
AMSDAQWAGMMIGDEAYAGSKNWLFLESTIKELFGFKHVVPTHQGRGAENLLSSIAIKPG
CCCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC
QYVAGNMYFTTTRYHQEKNGGIFVDIIRDEAHDASIDIPFKGNIDVNKLEKLIEEKGAEN
CEEECCEEEEEEEEEECCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
IAYVCLAVTVNLAGGQPVSMANMRAVRELTAKHGIKVFYDATRCVENAYFIKEQEEGYAD
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHEEEECCCCCCC
VSIKDIVHEMFSYSDGATMSGKKDGIVNIGGFLAINDEELFGKAKEIVVVYEGMPSYGGM
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEECCHHHHCCCEEEEEEEECCCCCCCC
TGRDMQAIAIGFREAMQYEYIEHRIRQVRYLGDRLKEAGVPIVEPVGGHAVFLDARRFCP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECHHHCC
HLDQEQFPAQSLAASLYIDSGVRSMERGIVSAGRDVNTGENHKPKLETVRLTIPRRVYTY
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEH
KHMDVVAESVIHLYKHKEDIRPLKFTYEPAQLRFFTAKFDYAD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCC
>Mature Secondary Structure
MDYMDLSKYPAEPFKIKSVEPVKMISREEREIAMKEAGYNTFNLRSEDVYIDLLTDSGTN
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCC
AMSDAQWAGMMIGDEAYAGSKNWLFLESTIKELFGFKHVVPTHQGRGAENLLSSIAIKPG
CCCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC
QYVAGNMYFTTTRYHQEKNGGIFVDIIRDEAHDASIDIPFKGNIDVNKLEKLIEEKGAEN
CEEECCEEEEEEEEEECCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
IAYVCLAVTVNLAGGQPVSMANMRAVRELTAKHGIKVFYDATRCVENAYFIKEQEEGYAD
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHEEEECCCCCCC
VSIKDIVHEMFSYSDGATMSGKKDGIVNIGGFLAINDEELFGKAKEIVVVYEGMPSYGGM
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEECCHHHHCCCEEEEEEEECCCCCCCC
TGRDMQAIAIGFREAMQYEYIEHRIRQVRYLGDRLKEAGVPIVEPVGGHAVFLDARRFCP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECHHHCC
HLDQEQFPAQSLAASLYIDSGVRSMERGIVSAGRDVNTGENHKPKLETVRLTIPRRVYTY
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEH
KHMDVVAESVIHLYKHKEDIRPLKFTYEPAQLRFFTAKFDYAD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]