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The map label for this gene is cbiK [H]

Identifier: 28210463

GI number: 28210463

Start: 767134

End: 767937

Strand: Direct

Name: cbiK [H]

Synonym: CTC00743

Alternate gene names: 28210463

Gene position: 767134-767937 (Clockwise)

Preceding gene: 28210462

Following gene: 28210464

Centisome position: 27.4

GC content: 28.11

Gene sequence:

>804_bases
ATGTCAAAGAAAGGTATCTTAGTAGTAAGTTTTGGGACAAGTTATGAGGAAACAAGAAAGGTTACAATTGAAGCTATAGA
AGATAGAATAAGAGAAACTTATAAGGATTATGAAATTAGAAGAGCATTCACTTCCGGAATGATAATTAATAAATTAAAAA
AGAGAGATAATATACATATAGATAATCCTGAAGAGGCTTTAGAGAAAATGGCTAAAGATGGGTTCGAAGAAGTTATAGTT
CAATCTCTACATATAATTCCAGGGGATGAATATGATGAAATAAAAGTAGCTGTTCATAGATTTAGAAATAAAAAAGTATT
TAAAAAACTTACACTAGGAAGACCTCTACTTTATAGAATAGAGGATTATTTCAATGTAGTAGAAGCTTTAAAACCACAAA
TACCTGAAACTAGAGAAGATTCTGCTGTGTTATTTATGGGACATGGAAGTGTACATTCTTCTAATTCATGTTATTCACAA
TTTCAATATGTGTTAAATGAAAGCGGACTAAAAAATATTTTTGTAGCTAATGTAGAAGGATTTCCAGAAATAGATGTAAT
TATATCTAAACTAAAAGAAGAAAATATAAAAGAAGTAACACTAATGCCTTTTATGATAGTAGCAGGAGATCATGCAACAA
ATGATATGGCAGGAGAAGATGAAGATTCTTGGAAGAATATATTAGAAAATGAAGGTTTTAAAATAAATATATATCTTCAT
GGATTAGGAGAAAATAAGGGAATACAAGATATCTATATCCAATATGTTCAAGATTGTATAGATGATAATCCTTTAAAAAA
ATAA

Upstream 100 bases:

>100_bases
CCTATGAAAATCTAAAGGATATACAAAATATAAATCTTTTAGGAGAAATAAAAAAAGGAACTTAAATATTATAAAGTCAA
AAGAAAGGAAAGATGTTTTT

Downstream 100 bases:

>100_bases
ATATAAACATTTAAACCTTTAATTTTTGACTAGGGAAAATAGTATTAAAAGTATTTAGAAATAATAGATATAAGTATTTA
TTTATGTTATATTAAGAGTA

Product: anaerobic cobalt chelatase cbiK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSKKGILVVSFGTSYEETRKVTIEAIEDRIRETYKDYEIRRAFTSGMIINKLKKRDNIHIDNPEEALEKMAKDGFEEVIV
QSLHIIPGDEYDEIKVAVHRFRNKKVFKKLTLGRPLLYRIEDYFNVVEALKPQIPETREDSAVLFMGHGSVHSSNSCYSQ
FQYVLNESGLKNIFVANVEGFPEIDVIISKLKEENIKEVTLMPFMIVAGDHATNDMAGEDEDSWKNILENEGFKINIYLH
GLGENKGIQDIYIQYVQDCIDDNPLKK

Sequences:

>Translated_267_residues
MSKKGILVVSFGTSYEETRKVTIEAIEDRIRETYKDYEIRRAFTSGMIINKLKKRDNIHIDNPEEALEKMAKDGFEEVIV
QSLHIIPGDEYDEIKVAVHRFRNKKVFKKLTLGRPLLYRIEDYFNVVEALKPQIPETREDSAVLFMGHGSVHSSNSCYSQ
FQYVLNESGLKNIFVANVEGFPEIDVIISKLKEENIKEVTLMPFMIVAGDHATNDMAGEDEDSWKNILENEGFKINIYLH
GLGENKGIQDIYIQYVQDCIDDNPLKK
>Mature_266_residues
SKKGILVVSFGTSYEETRKVTIEAIEDRIRETYKDYEIRRAFTSGMIINKLKKRDNIHIDNPEEALEKMAKDGFEEVIVQ
SLHIIPGDEYDEIKVAVHRFRNKKVFKKLTLGRPLLYRIEDYFNVVEALKPQIPETREDSAVLFMGHGSVHSSNSCYSQF
QYVLNESGLKNIFVANVEGFPEIDVIISKLKEENIKEVTLMPFMIVAGDHATNDMAGEDEDSWKNILENEGFKINIYLHG
LGENKGIQDIYIQYVQDCIDDNPLKK

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 30743; Mature: 30612

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKGILVVSFGTSYEETRKVTIEAIEDRIRETYKDYEIRRAFTSGMIINKLKKRDNIHI
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DNPEEALEKMAKDGFEEVIVQSLHIIPGDEYDEIKVAVHRFRNKKVFKKLTLGRPLLYRI
CCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EDYFNVVEALKPQIPETREDSAVLFMGHGSVHSSNSCYSQFQYVLNESGLKNIFVANVEG
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCC
FPEIDVIISKLKEENIKEVTLMPFMIVAGDHATNDMAGEDEDSWKNILENEGFKINIYLH
CCHHHHHHHHHHHCCCCEEEEHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEE
GLGENKGIQDIYIQYVQDCIDDNPLKK
ECCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKKGILVVSFGTSYEETRKVTIEAIEDRIRETYKDYEIRRAFTSGMIINKLKKRDNIHI
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DNPEEALEKMAKDGFEEVIVQSLHIIPGDEYDEIKVAVHRFRNKKVFKKLTLGRPLLYRI
CCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EDYFNVVEALKPQIPETREDSAVLFMGHGSVHSSNSCYSQFQYVLNESGLKNIFVANVEG
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCC
FPEIDVIISKLKEENIKEVTLMPFMIVAGDHATNDMAGEDEDSWKNILENEGFKINIYLH
CCHHHHHHHHHHHCCCCEEEEHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEE
GLGENKGIQDIYIQYVQDCIDDNPLKK
ECCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]