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The map label for this gene is cbiF [H]

Identifier: 28210457

GI number: 28210457

Start: 762457

End: 763227

Strand: Direct

Name: cbiF [H]

Synonym: CTC00736

Alternate gene names: 28210457

Gene position: 762457-763227 (Clockwise)

Preceding gene: 28210456

Following gene: 28210458

Centisome position: 27.24

GC content: 31.91

Gene sequence:

>771_bases
ATGAGTAAAATTGCTTCAAAAGTATATTTCATAGGTGCAGGACCTGGAGATGTGGATTTAATTACAGTAAAAGGAAGAGA
TATATTATCTGTAGCAGATGTAGTTATATATGCAGGTTCCCTAGTAAGTAAAGAACATTTAGATTTTTGTAAAGAAGATG
TAGAAAAACATAATTCAGCTTCCATGACTTTAGAAGATGTTATAGAAGTTATGAAAAAGGCTAAGGATGAAGGTAAATCA
GTAGTTAGACTTCATACAGGAGATCCATCTATGTATGGAGCAATAAAAGAACAAATGGATGAACTAGATAAGTTTAATAT
AGAATATGAAGTTGTTCCAGGAGTTAGTTCTTGTTTTGCAGCAGCAGCAGCTATAAAAAGAGAATTTACGTTACCCAATG
TAACTCAAACGCTAATACTTACAAGAGTTGAAGGAAGAACTCCAGTACCAGAGGGAGAAGATTTAGAAAAACTAGCATCC
GTTGGAGCATCTATGGCAATTTTTCTATCCATTAGCATGATAGAGAAAGTAGTAGAGAAATTAAGAAAAGGTTATGGAAA
AAATGTACCTATTGCTGTAATAGAAAGAGCTACTTGGGAAGATCAAAGAGTTATTATAGGTACTTTAGACGATATAGCAG
GGAAAGTAAAAGAAGCTAATATAACTAAATGTGCACAAATATTAGTTGGAGATTTTATAGATTGTGAATATGATAAAAGT
CTTCTATATGATAAAAGCTTTTCACATATGTTTAGAAAGGCAGAAAAATAA

Upstream 100 bases:

>100_bases
AAGGACAACAAATACTTAGAAATAGAGAAGAAATAATAGAAAATAAAGATTATATGTCTCTTATAATTGCAAATAGAGAG
TAAAGAAAGGAAGATATAGT

Downstream 100 bases:

>100_bases
TATATGAAAATAGCTATTATAACTATTACTGAAAACGGAGATAAAATTGCATCTATTATAAAAAAGTTTTTGAGAGCTAA
CGTAACTATATTTTCTAAAA

Product: precorrin-3 methylase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSKIASKVYFIGAGPGDVDLITVKGRDILSVADVVIYAGSLVSKEHLDFCKEDVEKHNSASMTLEDVIEVMKKAKDEGKS
VVRLHTGDPSMYGAIKEQMDELDKFNIEYEVVPGVSSCFAAAAAIKREFTLPNVTQTLILTRVEGRTPVPEGEDLEKLAS
VGASMAIFLSISMIEKVVEKLRKGYGKNVPIAVIERATWEDQRVIIGTLDDIAGKVKEANITKCAQILVGDFIDCEYDKS
LLYDKSFSHMFRKAEK

Sequences:

>Translated_256_residues
MSKIASKVYFIGAGPGDVDLITVKGRDILSVADVVIYAGSLVSKEHLDFCKEDVEKHNSASMTLEDVIEVMKKAKDEGKS
VVRLHTGDPSMYGAIKEQMDELDKFNIEYEVVPGVSSCFAAAAAIKREFTLPNVTQTLILTRVEGRTPVPEGEDLEKLAS
VGASMAIFLSISMIEKVVEKLRKGYGKNVPIAVIERATWEDQRVIIGTLDDIAGKVKEANITKCAQILVGDFIDCEYDKS
LLYDKSFSHMFRKAEK
>Mature_255_residues
SKIASKVYFIGAGPGDVDLITVKGRDILSVADVVIYAGSLVSKEHLDFCKEDVEKHNSASMTLEDVIEVMKKAKDEGKSV
VRLHTGDPSMYGAIKEQMDELDKFNIEYEVVPGVSSCFAAAAAIKREFTLPNVTQTLILTRVEGRTPVPEGEDLEKLASV
GASMAIFLSISMIEKVVEKLRKGYGKNVPIAVIERATWEDQRVIIGTLDDIAGKVKEANITKCAQILVGDFIDCEYDKSL
LYDKSFSHMFRKAEK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=222, Percent_Identity=27.9279279279279, Blast_Score=95, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322922, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28215; Mature: 28084

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIASKVYFIGAGPGDVDLITVKGRDILSVADVVIYAGSLVSKEHLDFCKEDVEKHNSA
CCCHHCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
SMTLEDVIEVMKKAKDEGKSVVRLHTGDPSMYGAIKEQMDELDKFNIEYEVVPGVSSCFA
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AAAAIKREFTLPNVTQTLILTRVEGRTPVPEGEDLEKLASVGASMAIFLSISMIEKVVEK
HHHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LRKGYGKNVPIAVIERATWEDQRVIIGTLDDIAGKVKEANITKCAQILVGDFIDCEYDKS
HHHHCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
LLYDKSFSHMFRKAEK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIASKVYFIGAGPGDVDLITVKGRDILSVADVVIYAGSLVSKEHLDFCKEDVEKHNSA
CCHHCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
SMTLEDVIEVMKKAKDEGKSVVRLHTGDPSMYGAIKEQMDELDKFNIEYEVVPGVSSCFA
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
AAAAIKREFTLPNVTQTLILTRVEGRTPVPEGEDLEKLASVGASMAIFLSISMIEKVVEK
HHHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LRKGYGKNVPIAVIERATWEDQRVIIGTLDDIAGKVKEANITKCAQILVGDFIDCEYDKS
HHHHCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
LLYDKSFSHMFRKAEK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]