Click here to switch to the map view.

The map label for this gene is cbiT [H]

Identifier: 28210455

GI number: 28210455

Start: 760567

End: 761781

Strand: Direct

Name: cbiT [H]

Synonym: CTC00734

Alternate gene names: 28210455

Gene position: 760567-761781 (Clockwise)

Preceding gene: 28210454

Following gene: 28210456

Centisome position: 27.17

GC content: 28.89

Gene sequence:

>1215_bases
ATGAATAATTTAGGAGGTGAAGAAATGGTTTATATAGTTGGAATAGGACCAGGTCATAAAGATTATATAATGCCTAAAGC
TATAGAGATATTAGAAAAAGTAGATATAATAGTAGGCTTTGGCAGAGCTATGGAAAGTATAGATTTTTTACCAACCAAGA
AAATAAAAGTGGGTAAAATAAAAGAGGTAGTAGAAGTATTAAATGAAAATTTAAATAAAAACATAGCGGTAGTGGCATCA
GGAGATCCTCTGTTTTTTGGTATTACAGAATATATAAAAAGAGAATATAAAAAAGATATAGAGATAATACCGGGTATTAG
TTCCTTTCAATATCTTATGGGAAAATTAAATAAAAGTTGGAATAATGCTTATTTAGGTAGCCTTCATGGAAGAGAAGATG
AATTTTTAAAAGTGGTAAAGAAAAATCCTATATCTATATGGCTTACAGATAGTAAAAATAGTCCAGATAAGCTAGCTAAA
TCATTGCTTGCAAATGGATTAAATCCATATATGTATGTGGGTGAGGAACTATCTTATGAAGGTGAAAGAATAGTGAAAGG
ACAAGCAGAGAAAATAAAGGATGAAAAATTCTTAAAACTTTCTGTGGTAATTATTGAAAATAATAATTTTATAGCAAAAG
AAGATATTCATTTTATAAAGGACAATGAATTCATAAGAGGAGATTGTCCTATGACAAAGGAAGAAGTAAGAATGCTTACT
GCTATTAAAATGGATATAAAAGAAGAAGATAAAGTTTTAGATATAGGAGCAGGAACTGGCTCTGTAAGTATTCAAGCATG
TAAACTATGTCCCTTAGGAAAAGTTATAGCAATAGAAAAAGAAGAAGAGGCCCTAAGGGTTATAAGAGAAAATAAGAAAA
AGTTTAATGCAAATAATTTAGAAATAGTAGAGGGTGAAGCTTTATCCATAAAAGACACAATACAGGAAAGCTTTAATTCT
ATATTTATAGGTGGAAGTGGCGGAAACCTAGAAGAGTTAATAAAAGAATATAGTAAAAAATTAAAAGATAATGGGAAAAT
AGTGCTTAATTTTATAACTATAAATAATTTATATAAGGCCATGGATACTTTAAAAGAACTAGGATTTAAGGTAAAGTGTA
CTCAAGTGTCCATAAGTAAGACTAGAGGGCAAACTTATATGTTAATGGGATATAATCCAATATTTATAATAGAAGGAGAA
AAGACAAATGGCTAA

Upstream 100 bases:

>100_bases
GCTAAAAGAATAGAAATATATAGTTATGGAGAAATCTCTCCAAAGATTTTAATGTTCTCCATGGAAAAAATTTTATTTAG
TGAATAACTAACAGTGAACA

Downstream 100 bases:

>100_bases
ATTATATGGAATAGGTGTAGGACCAGGAAGTGAAGAACTTTTAACAGTAAAAGCTGTTAATATATTGAAAAATGCAAAGG
TAGTTATTGCTCCATCTTCA

Product: precorrin-6B methylase/decarboxylase cbiT/cbiE

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNNLGGEEMVYIVGIGPGHKDYIMPKAIEILEKVDIIVGFGRAMESIDFLPTKKIKVGKIKEVVEVLNENLNKNIAVVAS
GDPLFFGITEYIKREYKKDIEIIPGISSFQYLMGKLNKSWNNAYLGSLHGREDEFLKVVKKNPISIWLTDSKNSPDKLAK
SLLANGLNPYMYVGEELSYEGERIVKGQAEKIKDEKFLKLSVVIIENNNFIAKEDIHFIKDNEFIRGDCPMTKEEVRMLT
AIKMDIKEEDKVLDIGAGTGSVSIQACKLCPLGKVIAIEKEEEALRVIRENKKKFNANNLEIVEGEALSIKDTIQESFNS
IFIGGSGGNLEELIKEYSKKLKDNGKIVLNFITINNLYKAMDTLKELGFKVKCTQVSISKTRGQTYMLMGYNPIFIIEGE
KTNG

Sequences:

>Translated_404_residues
MNNLGGEEMVYIVGIGPGHKDYIMPKAIEILEKVDIIVGFGRAMESIDFLPTKKIKVGKIKEVVEVLNENLNKNIAVVAS
GDPLFFGITEYIKREYKKDIEIIPGISSFQYLMGKLNKSWNNAYLGSLHGREDEFLKVVKKNPISIWLTDSKNSPDKLAK
SLLANGLNPYMYVGEELSYEGERIVKGQAEKIKDEKFLKLSVVIIENNNFIAKEDIHFIKDNEFIRGDCPMTKEEVRMLT
AIKMDIKEEDKVLDIGAGTGSVSIQACKLCPLGKVIAIEKEEEALRVIRENKKKFNANNLEIVEGEALSIKDTIQESFNS
IFIGGSGGNLEELIKEYSKKLKDNGKIVLNFITINNLYKAMDTLKELGFKVKCTQVSISKTRGQTYMLMGYNPIFIIEGE
KTNG
>Mature_404_residues
MNNLGGEEMVYIVGIGPGHKDYIMPKAIEILEKVDIIVGFGRAMESIDFLPTKKIKVGKIKEVVEVLNENLNKNIAVVAS
GDPLFFGITEYIKREYKKDIEIIPGISSFQYLMGKLNKSWNNAYLGSLHGREDEFLKVVKKNPISIWLTDSKNSPDKLAK
SLLANGLNPYMYVGEELSYEGERIVKGQAEKIKDEKFLKLSVVIIENNNFIAKEDIHFIKDNEFIRGDCPMTKEEVRMLT
AIKMDIKEEDKVLDIGAGTGSVSIQACKLCPLGKVIAIEKEEEALRVIRENKKKFNANNLEIVEGEALSIKDTIQESFNS
IFIGGSGGNLEELIKEYSKKLKDNGKIVLNFITINNLYKAMDTLKELGFKVKCTQVSISKTRGQTYMLMGYNPIFIIEGE
KTNG

Specific function: Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12 [H]

COG id: COG2242

COG function: function code H; Precorrin-6B methylase 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Archaeal-type CbiT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014008
- InterPro:   IPR002935
- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 45398; Mature: 45398

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLGGEEMVYIVGIGPGHKDYIMPKAIEILEKVDIIVGFGRAMESIDFLPTKKIKVGKI
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCHHHHHCCCCCCCCEEHHHH
KEVVEVLNENLNKNIAVVASGDPLFFGITEYIKREYKKDIEIIPGISSFQYLMGKLNKSW
HHHHHHHHHCCCCCEEEEECCCCEEECHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCC
NNAYLGSLHGREDEFLKVVKKNPISIWLTDSKNSPDKLAKSLLANGLNPYMYVGEELSYE
CCCEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCC
GERIVKGQAEKIKDEKFLKLSVVIIENNNFIAKEDIHFIKDNEFIRGDCPMTKEEVRMLT
CCEEECCCHHHCCCCEEEEEEEEEEECCCEEEECCCEEECCCCEEECCCCCCHHHHHHHH
AIKMDIKEEDKVLDIGAGTGSVSIQACKLCPLGKVIAIEKEEEALRVIRENKKKFNANNL
HHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCE
EIVEGEALSIKDTIQESFNSIFIGGSGGNLEELIKEYSKKLKDNGKIVLNFITINNLYKA
EEECCCEEEHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
MDTLKELGFKVKCTQVSISKTRGQTYMLMGYNPIFIIEGEKTNG
HHHHHHCCCEEEEEEEEEECCCCCEEEEEECCEEEEEECCCCCC
>Mature Secondary Structure
MNNLGGEEMVYIVGIGPGHKDYIMPKAIEILEKVDIIVGFGRAMESIDFLPTKKIKVGKI
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCHHHHHCCCCCCCCEEHHHH
KEVVEVLNENLNKNIAVVASGDPLFFGITEYIKREYKKDIEIIPGISSFQYLMGKLNKSW
HHHHHHHHHCCCCCEEEEECCCCEEECHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCC
NNAYLGSLHGREDEFLKVVKKNPISIWLTDSKNSPDKLAKSLLANGLNPYMYVGEELSYE
CCCEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCC
GERIVKGQAEKIKDEKFLKLSVVIIENNNFIAKEDIHFIKDNEFIRGDCPMTKEEVRMLT
CCEEECCCHHHCCCCEEEEEEEEEEECCCEEEECCCEEECCCCEEECCCCCCHHHHHHHH
AIKMDIKEEDKVLDIGAGTGSVSIQACKLCPLGKVIAIEKEEEALRVIRENKKKFNANNL
HHHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCE
EIVEGEALSIKDTIQESFNSIFIGGSGGNLEELIKEYSKKLKDNGKIVLNFITINNLYKA
EEECCCEEEHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
MDTLKELGFKVKCTQVSISKTRGQTYMLMGYNPIFIIEGEKTNG
HHHHHHCCCEEEEEEEEEECCCCCEEEEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA