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The map label for this gene is hemA

Identifier: 28210446

GI number: 28210446

Start: 751072

End: 752271

Strand: Direct

Name: hemA

Synonym: CTC00725

Alternate gene names: 28210446

Gene position: 751072-752271 (Clockwise)

Preceding gene: 28210445

Following gene: 28210447

Centisome position: 26.83

GC content: 28.75

Gene sequence:

>1200_bases
ATGATAGGCGTTTTAGGAGTAAAAACTGAAGTAAAATTAGATATAAGAGAAAAACTTTCAATAATTCCTAAAAGATATGA
AAGTTCACTGATGAAATTGAAAGAAAAGTGTGATGAAGTTGTAATTTTGAGTACTTGTAATAGAACAGAAGTATATTATA
GCTATAAAAAACATAATGAAAATATAATTTCACACATATTTAAATGCTTAAATTGGGATGAAAACTATAAAAAATACACT
TTTCACTATAAAGACGATGAAGCTATAAATCATCTTATGAGGGTTATTTGTGGATTTGATTCCTTAATATTGGGAGAGGA
TCAAATATTAAGTCAAATTAAACAGGCTTATGAAATAGCACTAAGTAGTAAGAGTGTAAACAGGGATTTAAATAAATTAT
TTCAAATGGCCATAACCTGTGGCAAAGAATTTAGAAATAAATCTCAATTATATAAAATACCTGTATCCTCTGCTTCAATA
GCAGTAAATGAAGGAAGAAAACATGGAGGAAAAAACTTCATGGTACTAGGATATGGGGCAGTTGGAAGCCTAGCTGTTAA
ATACATATTGTCAGGTGAATTTGATAAATTGTACATAGTAGTGAGAAACCCTTCTGTTGTAGATTTAAGGGATGATAGAA
TGACTATAATAAATTTTAATGAAAGAGGAAAATATTATAAAGATGTAGATTGTATAATAAGTTGTACTTCAGCGCCCCAC
GTAGTAGTAGATGCCTTTGAACTTCCAAAACATAAAAATATAGTGGTATATGATTTAGCAGTACCAAGGGATGTAGACGA
GGAAGTTAGATATATGAAAAATGTAAAATTATATGATATAGATAATATAAGCTGTATAAATGACGAAAATTGTAGCAAAA
GAGAGGACATCATGAATAACAATTTATTTGTAATAGAAAAATATGAAAGAGAATTTGAGAAGTGGAGAAAAACTTCAGAA
ATATCTCCTCATATAGTTAATTTAAAGAAAAAAGGTGATGAAGTTTATAAAAAAAGATTAACCACCTTTAAAAATAAAAA
GTATACGAAAGATGTAGACGCTTTAGCAGAAATTATGCTCAAAAGTACATCTAATGCGTATATAAATAAGGCTATTGAAG
TATTAAAAGAAGAACAGTTGAAGGGAAGAGGAGAGGAGTGCTTGAGAATAATAGAAAAGATATTTTACTCCGCCCAATAG

Upstream 100 bases:

>100_bases
CATATAATTTATTTTTAATAATTTCGATTTTATTATTTAACAATAGATTATAGATAGAGTATTATTTAATATAGAGTAAT
TTTATGGAGATGATAATTTT

Downstream 100 bases:

>100_bases
AATATTCTTTTATATCCTTACTATCTAATAAAATAAAAGTATTAATAGTAGGTGGAGGAAGAGCAGGTTTTATAAAAGCT
AAGACTCTTAGTAATAATGG

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MIGVLGVKTEVKLDIREKLSIIPKRYESSLMKLKEKCDEVVILSTCNRTEVYYSYKKHNENIISHIFKCLNWDENYKKYT
FHYKDDEAINHLMRVICGFDSLILGEDQILSQIKQAYEIALSSKSVNRDLNKLFQMAITCGKEFRNKSQLYKIPVSSASI
AVNEGRKHGGKNFMVLGYGAVGSLAVKYILSGEFDKLYIVVRNPSVVDLRDDRMTIINFNERGKYYKDVDCIISCTSAPH
VVVDAFELPKHKNIVVYDLAVPRDVDEEVRYMKNVKLYDIDNISCINDENCSKREDIMNNNLFVIEKYEREFEKWRKTSE
ISPHIVNLKKKGDEVYKKRLTTFKNKKYTKDVDALAEIMLKSTSNAYINKAIEVLKEEQLKGRGEECLRIIEKIFYSAQ

Sequences:

>Translated_399_residues
MIGVLGVKTEVKLDIREKLSIIPKRYESSLMKLKEKCDEVVILSTCNRTEVYYSYKKHNENIISHIFKCLNWDENYKKYT
FHYKDDEAINHLMRVICGFDSLILGEDQILSQIKQAYEIALSSKSVNRDLNKLFQMAITCGKEFRNKSQLYKIPVSSASI
AVNEGRKHGGKNFMVLGYGAVGSLAVKYILSGEFDKLYIVVRNPSVVDLRDDRMTIINFNERGKYYKDVDCIISCTSAPH
VVVDAFELPKHKNIVVYDLAVPRDVDEEVRYMKNVKLYDIDNISCINDENCSKREDIMNNNLFVIEKYEREFEKWRKTSE
ISPHIVNLKKKGDEVYKKRLTTFKNKKYTKDVDALAEIMLKSTSNAYINKAIEVLKEEQLKGRGEECLRIIEKIFYSAQ
>Mature_399_residues
MIGVLGVKTEVKLDIREKLSIIPKRYESSLMKLKEKCDEVVILSTCNRTEVYYSYKKHNENIISHIFKCLNWDENYKKYT
FHYKDDEAINHLMRVICGFDSLILGEDQILSQIKQAYEIALSSKSVNRDLNKLFQMAITCGKEFRNKSQLYKIPVSSASI
AVNEGRKHGGKNFMVLGYGAVGSLAVKYILSGEFDKLYIVVRNPSVVDLRDDRMTIINFNERGKYYKDVDCIISCTSAPH
VVVDAFELPKHKNIVVYDLAVPRDVDEEVRYMKNVKLYDIDNISCINDENCSKREDIMNNNLFVIEKYEREFEKWRKTSE
ISPHIVNLKKKGDEVYKKRLTTFKNKKYTKDVDALAEIMLKSTSNAYINKAIEVLKEEQLKGRGEECLRIIEKIFYSAQ

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=394, Percent_Identity=25.3807106598985, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_CLOTE (Q897K9)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781390.1
- ProteinModelPortal:   Q897K9
- SMR:   Q897K9
- GeneID:   1058948
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00725
- NMPDR:   fig|212717.1.peg.612
- HOGENOM:   HBG312333
- OMA:   EVRPEHF
- PhylomeDB:   Q897K9
- ProtClustDB:   PRK00045
- BioCyc:   CTET212717:CTC_00725-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 46358; Mature: 46358

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 46-46 BINDING 101-101 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVLGVKTEVKLDIREKLSIIPKRYESSLMKLKEKCDEVVILSTCNRTEVYYSYKKHNE
CEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHCCH
NIISHIFKCLNWDENYKKYTFHYKDDEAINHLMRVICGFDSLILGEDQILSQIKQAYEIA
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHH
LSSKSVNRDLNKLFQMAITCGKEFRNKSQLYKIPVSSASIAVNEGRKHGGKNFMVLGYGA
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCC
VGSLAVKYILSGEFDKLYIVVRNPSVVDLRDDRMTIINFNERGKYYKDVDCIISCTSAPH
HHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCEEEEEECCCCCEEECCEEEEECCCCCE
VVVDAFELPKHKNIVVYDLAVPRDVDEEVRYMKNVKLYDIDNISCINDENCSKREDIMNN
EEEEHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEECCCCEECCCCCCCHHHHHCCC
NLFVIEKYEREFEKWRKTSEISPHIVNLKKKGDEVYKKRLTTFKNKKYTKDVDALAEIML
CEEEEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KSTSNAYINKAIEVLKEEQLKGRGEECLRIIEKIFYSAQ
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGVLGVKTEVKLDIREKLSIIPKRYESSLMKLKEKCDEVVILSTCNRTEVYYSYKKHNE
CEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHCCH
NIISHIFKCLNWDENYKKYTFHYKDDEAINHLMRVICGFDSLILGEDQILSQIKQAYEIA
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHHHH
LSSKSVNRDLNKLFQMAITCGKEFRNKSQLYKIPVSSASIAVNEGRKHGGKNFMVLGYGA
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCC
VGSLAVKYILSGEFDKLYIVVRNPSVVDLRDDRMTIINFNERGKYYKDVDCIISCTSAPH
HHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCEEEEEECCCCCEEECCEEEEECCCCCE
VVVDAFELPKHKNIVVYDLAVPRDVDEEVRYMKNVKLYDIDNISCINDENCSKREDIMNN
EEEEHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEECCCCEECCCCCCCHHHHHCCC
NLFVIEKYEREFEKWRKTSEISPHIVNLKKKGDEVYKKRLTTFKNKKYTKDVDALAEIML
CEEEEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KSTSNAYINKAIEVLKEEQLKGRGEECLRIIEKIFYSAQ
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129