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The map label for this gene is cobD [H]

Identifier: 28210442

GI number: 28210442

Start: 747211

End: 748209

Strand: Direct

Name: cobD [H]

Synonym: CTC00721

Alternate gene names: 28210442

Gene position: 747211-748209 (Clockwise)

Preceding gene: 28210441

Following gene: 28210443

Centisome position: 26.69

GC content: 32.63

Gene sequence:

>999_bases
TTGGCATGTAAGAAAGAGGTGGTTTCCATTAAAACTCCAATTTTAATGGGATTAATTGATATAATAGTTGCAGTTTTATT
AGACTTTGCAATAGGCGACCCGTATTGGTTTCCACATCCTGTAATATATATAGGGAAACTTATAAGCTATTTGGAAGAAG
TGGGAAGAAAGCATTTTAAAAGTAATAAAGGACTTAAAACTTTAGGAGGATTAGTTGTTCTCACTATAGCTATTACATCC
TTTGGAATACCATTTTTAATTCTTTGGATGGTAAAGGATAGTTTTTGGATATTCCATTTTTTAAATATAATTTTAATCTG
GACAACTTTAGCAGCAAAGTCCTTAAAAGTAGAAGGAAAAAGGGTATATTACGCTTTGAAAAATGAAGATATTCAAGAGG
CAAGGGAAAAGCTGTCTTACATAGTGGGAAGAGATACAAGAAATCTTACGGAAGAGGAAATAATAAGGGCTGATATAGAA
ACTATAATGGAAAATACTGCAGATGGTGTAATAGCTCCACTATTTTATGCAATGATAGGAGGAGCACCTTTTGCAATGAT
GTATAAAGGTATAAACACTATGGATTCAATGCTTGGATATATGAATGATAAATATATTCATTTAGGATTTTTCCCAGCTA
AGGTAGATGATGTATTTAATTTTATACCAGCTAGGATTTCTGGTGTTTTAATATGCCTATCTGCACCAATAGTAAAGGGA
AATATAATAAGAAGTTTTAAAGTAATGTTAAGGGATAGAAAAAACCATAAAAGCCCTAACTGTGCTTATCCGGAGGGAGC
AGGTGCTGGGGTTATGGGTATACAGCTTGGTGGTACAAATGTTTATTTTGGTAAAGCAGTATATAAACCCACTATAGGAG
ATAGAATTAAAGATTTACACCATGAACTTATAAACGACAGTGTAAAGCTTATGTATGCTTCAGAGACGCTTATGGTTATT
ATATATGCTCTTACAGTTACTAGTTATAATTTGAGGTGA

Upstream 100 bases:

>100_bases
AGAAATGCAATAAATTATGAAAATCTAAGAGAAAAGAATTTAGATATGCTAGCTGATTTAGTTAGAGAAAATATAGATAT
GGATTACATATATAAAATAA

Downstream 100 bases:

>100_bases
TGACATTGAGACATGGAGGAGATATATACACTGATGGAATATTAAAAGGAAGGAAACTTATAGATTTTAGCTCTAATATA
AACCCCCTTGGTATACCAAA

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MACKKEVVSIKTPILMGLIDIIVAVLLDFAIGDPYWFPHPVIYIGKLISYLEEVGRKHFKSNKGLKTLGGLVVLTIAITS
FGIPFLILWMVKDSFWIFHFLNIILIWTTLAAKSLKVEGKRVYYALKNEDIQEAREKLSYIVGRDTRNLTEEEIIRADIE
TIMENTADGVIAPLFYAMIGGAPFAMMYKGINTMDSMLGYMNDKYIHLGFFPAKVDDVFNFIPARISGVLICLSAPIVKG
NIIRSFKVMLRDRKNHKSPNCAYPEGAGAGVMGIQLGGTNVYFGKAVYKPTIGDRIKDLHHELINDSVKLMYASETLMVI
IYALTVTSYNLR

Sequences:

>Translated_332_residues
MACKKEVVSIKTPILMGLIDIIVAVLLDFAIGDPYWFPHPVIYIGKLISYLEEVGRKHFKSNKGLKTLGGLVVLTIAITS
FGIPFLILWMVKDSFWIFHFLNIILIWTTLAAKSLKVEGKRVYYALKNEDIQEAREKLSYIVGRDTRNLTEEEIIRADIE
TIMENTADGVIAPLFYAMIGGAPFAMMYKGINTMDSMLGYMNDKYIHLGFFPAKVDDVFNFIPARISGVLICLSAPIVKG
NIIRSFKVMLRDRKNHKSPNCAYPEGAGAGVMGIQLGGTNVYFGKAVYKPTIGDRIKDLHHELINDSVKLMYASETLMVI
IYALTVTSYNLR
>Mature_331_residues
ACKKEVVSIKTPILMGLIDIIVAVLLDFAIGDPYWFPHPVIYIGKLISYLEEVGRKHFKSNKGLKTLGGLVVLTIAITSF
GIPFLILWMVKDSFWIFHFLNIILIWTTLAAKSLKVEGKRVYYALKNEDIQEAREKLSYIVGRDTRNLTEEEIIRADIET
IMENTADGVIAPLFYAMIGGAPFAMMYKGINTMDSMLGYMNDKYIHLGFFPAKVDDVFNFIPARISGVLICLSAPIVKGN
IIRSFKVMLRDRKNHKSPNCAYPEGAGAGVMGIQLGGTNVYFGKAVYKPTIGDRIKDLHHELINDSVKLMYASETLMVII
YALTVTSYNLR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 37264; Mature: 37132

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACKKEVVSIKTPILMGLIDIIVAVLLDFAIGDPYWFPHPVIYIGKLISYLEEVGRKHFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SNKGLKTLGGLVVLTIAITSFGIPFLILWMVKDSFWIFHFLNIILIWTTLAAKSLKVEGK
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
RVYYALKNEDIQEAREKLSYIVGRDTRNLTEEEIIRADIETIMENTADGVIAPLFYAMIG
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GAPFAMMYKGINTMDSMLGYMNDKYIHLGFFPAKVDDVFNFIPARISGVLICLSAPIVKG
CCHHHHHHHCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHEEHEEECCCCCC
NIIRSFKVMLRDRKNHKSPNCAYPEGAGAGVMGIQLGGTNVYFGKAVYKPTIGDRIKDLH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCEEECCCHHHHHHHHH
HELINDSVKLMYASETLMVIIYALTVTSYNLR
HHHHCCCEEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ACKKEVVSIKTPILMGLIDIIVAVLLDFAIGDPYWFPHPVIYIGKLISYLEEVGRKHFK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SNKGLKTLGGLVVLTIAITSFGIPFLILWMVKDSFWIFHFLNIILIWTTLAAKSLKVEGK
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
RVYYALKNEDIQEAREKLSYIVGRDTRNLTEEEIIRADIETIMENTADGVIAPLFYAMIG
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GAPFAMMYKGINTMDSMLGYMNDKYIHLGFFPAKVDDVFNFIPARISGVLICLSAPIVKG
CCHHHHHHHCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHEEHEEECCCCCC
NIIRSFKVMLRDRKNHKSPNCAYPEGAGAGVMGIQLGGTNVYFGKAVYKPTIGDRIKDLH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCEEECCCHHHHHHHHH
HELINDSVKLMYASETLMVIIYALTVTSYNLR
HHHHCCCEEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]