BacMap

Definition	Clostridium tetani E88 chromosome, complete genome.
Accession	NC_004557
Length	2,799,251

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The map label for this gene is ppiB [H]

Identifier: 28210402

GI number: 28210402

Start: 695073

End: 695591

Strand: Direct

Name: ppiB [H]

Synonym: CTC00678

Alternate gene names: 28210402

Gene position: 695073-695591 (Clockwise)

Preceding gene: 28210401

Following gene: 28210404

Centisome position: 24.83

GC content: 34.3

Gene sequence:

>519_bases
ATGAAAAATCCTATAATTACTATAACAATGGAAAATGGAGATGTTATGAAAGGAGAACTATATCCTGAAATAGCTCCAAA
TACTGTTAGAAACTTTATAAGCTTAATAGATAAAGGCTTTTATGATGGGTTAATTTTTCACAGGGTAATACCTGGATTTG
TAATACAAGGTGGATGCCCAGAAGGAACAGGAGTTGGAGGACCTGGATACTCTATTAAAGGAGAATTTTCAGCTAACGGA
TTTCCAAATTCACTTAAACATGAAGAAGGAGTTTTATCCATGGCAAGAGCTATGAGTCCTGATTCAGCAGGAAGCCAATT
TTTTATAATGGTGGGAGAATCACCTCATTTAGATGGACAATATGCTGGTTTTGGAAAGATAACAGAAGGATTGGACGTAG
CATTTAAAATAGTAGAACAACCAACAGATTTTATGGATAAACCTTTAGAAGATCAAAAAATAAAGGAAATAACTGTAGAT
ACCTTTGGAGAAAAATACGAAGAACCTGAGGAAGCATAA

Upstream 100 bases:

>100_bases
ATATAGAAGTTTTTTTAAATACTGCAAAAAAAGAAAGTACTAAAAAATAATAAAAAATGCTATAATTAACAAGATAACTG
ATGACAAAGGAGAGGTTTAA

Downstream 100 bases:

>100_bases
TATAAAAATAGGACTGTATCTTATATATGTTAGATACAGTCCTATAATTTATTTAAAAAAAGAATTGTAACTTCCATCAG
AAGGCATTATAAAATCTCCT

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase B; Rotamase B [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKNPIITITMENGDVMKGELYPEIAPNTVRNFISLIDKGFYDGLIFHRVIPGFVIQGGCPEGTGVGGPGYSIKGEFSANG
FPNSLKHEEGVLSMARAMSPDSAGSQFFIMVGESPHLDGQYAGFGKITEGLDVAFKIVEQPTDFMDKPLEDQKIKEITVD
TFGEKYEEPEEA

Sequences:

>Translated_172_residues
MKNPIITITMENGDVMKGELYPEIAPNTVRNFISLIDKGFYDGLIFHRVIPGFVIQGGCPEGTGVGGPGYSIKGEFSANG
FPNSLKHEEGVLSMARAMSPDSAGSQFFIMVGESPHLDGQYAGFGKITEGLDVAFKIVEQPTDFMDKPLEDQKIKEITVD
TFGEKYEEPEEA
>Mature_172_residues
MKNPIITITMENGDVMKGELYPEIAPNTVRNFISLIDKGFYDGLIFHRVIPGFVIQGGCPEGTGVGGPGYSIKGEFSANG
FPNSLKHEEGVLSMARAMSPDSAGSQFFIMVGESPHLDGQYAGFGKITEGLDVAFKIVEQPTDFMDKPLEDQKIKEITVD
TFGEKYEEPEEA

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=156, Percent_Identity=41.6666666666667, Blast_Score=111, Evalue=4e-25,
Organism=Homo sapiens, GI24308049, Length=159, Percent_Identity=42.1383647798742, Blast_Score=101, Evalue=3e-22,
Organism=Homo sapiens, GI7706339, Length=137, Percent_Identity=40.8759124087591, Blast_Score=100, Evalue=6e-22,
Organism=Homo sapiens, GI64276486, Length=143, Percent_Identity=39.8601398601399, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI4758950, Length=140, Percent_Identity=45, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI4505991, Length=142, Percent_Identity=44.3661971830986, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI20911035, Length=159, Percent_Identity=37.1069182389937, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI42560244, Length=148, Percent_Identity=37.1621621621622, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI10863927, Length=152, Percent_Identity=41.4473684210526, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310127646, Length=145, Percent_Identity=41.3793103448276, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310114371, Length=145, Percent_Identity=41.3793103448276, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI310119221, Length=145, Percent_Identity=41.3793103448276, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI6631100, Length=170, Percent_Identity=33.5294117647059, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI22547212, Length=156, Percent_Identity=41.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI7657473, Length=156, Percent_Identity=41.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI22547215, Length=156, Percent_Identity=41.6666666666667, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI5031987, Length=129, Percent_Identity=44.9612403100775, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI5454154, Length=145, Percent_Identity=39.3103448275862, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI45439316, Length=150, Percent_Identity=39.3333333333333, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI303305009, Length=174, Percent_Identity=36.2068965517241, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI5174637, Length=150, Percent_Identity=39.3333333333333, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI256017253, Length=154, Percent_Identity=38.961038961039, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI221139811, Length=154, Percent_Identity=38.961038961039, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI256017251, Length=154, Percent_Identity=38.961038961039, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI4826932, Length=137, Percent_Identity=41.6058394160584, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI310110104, Length=120, Percent_Identity=43.3333333333333, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI178057341, Length=121, Percent_Identity=42.1487603305785, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI310129267, Length=120, Percent_Identity=42.5, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI310120620, Length=120, Percent_Identity=42.5, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI209417936, Length=121, Percent_Identity=41.3223140495868, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI30102944, Length=121, Percent_Identity=41.3223140495868, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI219842199, Length=121, Percent_Identity=41.3223140495868, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI150418007, Length=127, Percent_Identity=40.9448818897638, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI310132444, Length=124, Percent_Identity=41.1290322580645, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI310113152, Length=124, Percent_Identity=41.1290322580645, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI169163387, Length=156, Percent_Identity=36.5384615384615, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI169164185, Length=156, Percent_Identity=36.5384615384615, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI169163623, Length=156, Percent_Identity=36.5384615384615, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI14277126, Length=159, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789763, Length=146, Percent_Identity=42.4657534246575, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786736, Length=169, Percent_Identity=36.6863905325444, Blast_Score=87, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539496, Length=131, Percent_Identity=44.2748091603053, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71980594, Length=155, Percent_Identity=40, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71980590, Length=149, Percent_Identity=40.2684563758389, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17506311, Length=143, Percent_Identity=39.1608391608392, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17552780, Length=141, Percent_Identity=43.9716312056738, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71997782, Length=132, Percent_Identity=40.1515151515151, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551284, Length=142, Percent_Identity=40.8450704225352, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551282, Length=142, Percent_Identity=40.8450704225352, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506307, Length=151, Percent_Identity=45.0331125827815, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559074, Length=154, Percent_Identity=39.6103896103896, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17532641, Length=154, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17566266, Length=134, Percent_Identity=41.7910447761194, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17539498, Length=130, Percent_Identity=39.2307692307692, Blast_Score=76, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17552782, Length=145, Percent_Identity=40.6896551724138, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32563659, Length=155, Percent_Identity=34.8387096774194, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565860, Length=134, Percent_Identity=41.7910447761194, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17552784, Length=145, Percent_Identity=36.551724137931, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17532643, Length=163, Percent_Identity=35.5828220858896, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321848, Length=149, Percent_Identity=43.6241610738255, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323562, Length=143, Percent_Identity=41.958041958042, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320359, Length=127, Percent_Identity=44.8818897637795, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323246, Length=170, Percent_Identity=37.0588235294118, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320510, Length=144, Percent_Identity=40.9722222222222, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17986117, Length=145, Percent_Identity=42.0689655172414, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922376, Length=153, Percent_Identity=39.8692810457516, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652460, Length=156, Percent_Identity=33.974358974359, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI45549139, Length=129, Percent_Identity=45.7364341085271, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130249, Length=138, Percent_Identity=43.4782608695652, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922912, Length=154, Percent_Identity=40.2597402597403, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357249, Length=138, Percent_Identity=34.0579710144928, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24664125, Length=129, Percent_Identity=44.1860465116279, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357783, Length=129, Percent_Identity=44.1860465116279, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24650807, Length=148, Percent_Identity=33.7837837837838, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136360, Length=142, Percent_Identity=35.9154929577465, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24586038, Length=164, Percent_Identity=34.7560975609756, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355677, Length=162, Percent_Identity=31.4814814814815, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18758; Mature: 18758

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPIITITMENGDVMKGELYPEIAPNTVRNFISLIDKGFYDGLIFHRVIPGFVIQGGCP
CCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
EGTGVGGPGYSIKGEFSANGFPNSLKHEEGVLSMARAMSPDSAGSQFFIMVGESPHLDGQ
CCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
YAGFGKITEGLDVAFKIVEQPTDFMDKPLEDQKIKEITVDTFGEKYEEPEEA
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNPIITITMENGDVMKGELYPEIAPNTVRNFISLIDKGFYDGLIFHRVIPGFVIQGGCP
CCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
EGTGVGGPGYSIKGEFSANGFPNSLKHEEGVLSMARAMSPDSAGSQFFIMVGESPHLDGQ
CCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
YAGFGKITEGLDVAFKIVEQPTDFMDKPLEDQKIKEITVDTFGEKYEEPEEA
CCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 8022278; 9384377; 8755892 [H]