Click here to switch to the map view.

The map label for this gene is spaK [H]

Identifier: 28210359

GI number: 28210359

Start: 647388

End: 648752

Strand: Direct

Name: spaK [H]

Synonym: CTC00629

Alternate gene names: 28210359

Gene position: 647388-648752 (Clockwise)

Preceding gene: 28210358

Following gene: 28210360

Centisome position: 23.13

GC content: 23.88

Gene sequence:

>1365_bases
GTGGAAAGAATAAAGAAACAGAAAAGTATTTCTAAAATTTTTGCAATATATGTGGTTTTGTTTTGTATAGTTACAATTAT
TGTGGGAGTAATTAATTTAAGTTTATTTATGATTGGTTTAAATAAAGGTTTTATATTACCAGCAAATTATTATGAACGAC
GAATTGAAAATCAAAGAAGTGAAATAGCTAAAGCTAAAGATGTAAAGAAATTAATACCTGAAGAATGTAAGTATGCAGTT
TATGATTTAAATGGAAAAGTCCTTCAAGGAAATGTTTCACAAGATAAAGCTGTAGGTATGTGGAATATTGTGAAAAGTAA
TAAAATAAGCAATGGAAGGTATTTTTATAAGATTATTCAAAGGTATAATGGAATTTGTGTTGTGGAATATAGAATTAATG
TAAGTTTTAAAAATCCAACTTTAAGAAAATATATTAAAACTCCTGAAGTTACTTTTGAGTTATTATTACTTGGTTTATTT
ATTTTTGAAATTTTAATTTTTTCAAGGTCTTTTAATAAGAGGTTAAAAAAGGAAATAAAAATTTTAAAGGATACTACAGA
AAATATACAAATGGAAAACTTAGAGTTTAAAGTAAAATACTCTAATATAGTAGAAATAAATGATGTAATTAATGCATTAG
ATAAGATGAAAACTAAGCTCAGTGAGTCATTAACTAAGCAGTGGGATATGGAAGAAACTCGCAAGGAACAAATAGGAGCG
TTGGCTCATGATATAAAAACACCTCTAACAATAATCAGAGGAAATTCAGAACTTTTAAAAGAATCAGATTTAAATGATGA
GCAGACTAAATTTAATGATGATATTTTAAATGAAATTAAAAATATGGAATTTTATATTAAATCTTTAATAGAAATTACAA
GGTCAGAAAAAGAACCTGCAATAGAAAAAAAGCAAGTAAACTTAATAAAATTTATAGATAATATAATGAAGACAGGACAT
TTGATTAGTAAAAATAAGAATTTAAGCTTTAAAAGTAGAATTAAAAATATTCCAGAATTTATTTTTATAGATGAAATAGC
ACTGAAACGAGTTATAAATAATATTATTTCAAATTCTGTTGATTATTCTTTAGAAAATGGAGAAATATTATTAACTGTTG
ATTCTGATGATAAAAATATACAATTTATAATTGAAGATTCAGGAAAAGGATTTACAAAAGAAGAAATAAGTTTTGCAACA
AAGCAGTTTTTTCAAGGAGATAAAAGTAGAAATTCAAAAAATCATTATGGAATGGGATTATATATTGCAGAAAAAATTAT
AGAAAAACATAATGGAAGTATAATTCTAGAGAACTCCAAATTACTTAAAGGTGCAAAGGTTATATTGAATTTACCAAATA
GTTAA

Upstream 100 bases:

>100_bases
GTGATACCACAGCAATTGTAGAACATATTAAAAACATACGAAATAAGTTAAAAAATATGGGGATAAATCCAATAGAAACA
GTTTGGGGGATAGGGTATAA

Downstream 100 bases:

>100_bases
AGAACAACTAGGAGAAGAAGGATTAAAGAAAGCGCTTATTATTGTAGTAGGTAAAAAGAATATTTATACTGAAGTAGAGT
AACTTTATTATACTTAAATA

Product: subtilin biosynthesis sensor protein spaK

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MERIKKQKSISKIFAIYVVLFCIVTIIVGVINLSLFMIGLNKGFILPANYYERRIENQRSEIAKAKDVKKLIPEECKYAV
YDLNGKVLQGNVSQDKAVGMWNIVKSNKISNGRYFYKIIQRYNGICVVEYRINVSFKNPTLRKYIKTPEVTFELLLLGLF
IFEILIFSRSFNKRLKKEIKILKDTTENIQMENLEFKVKYSNIVEINDVINALDKMKTKLSESLTKQWDMEETRKEQIGA
LAHDIKTPLTIIRGNSELLKESDLNDEQTKFNDDILNEIKNMEFYIKSLIEITRSEKEPAIEKKQVNLIKFIDNIMKTGH
LISKNKNLSFKSRIKNIPEFIFIDEIALKRVINNIISNSVDYSLENGEILLTVDSDDKNIQFIIEDSGKGFTKEEISFAT
KQFFQGDKSRNSKNHYGMGLYIAEKIIEKHNGSIILENSKLLKGAKVILNLPNS

Sequences:

>Translated_454_residues
MERIKKQKSISKIFAIYVVLFCIVTIIVGVINLSLFMIGLNKGFILPANYYERRIENQRSEIAKAKDVKKLIPEECKYAV
YDLNGKVLQGNVSQDKAVGMWNIVKSNKISNGRYFYKIIQRYNGICVVEYRINVSFKNPTLRKYIKTPEVTFELLLLGLF
IFEILIFSRSFNKRLKKEIKILKDTTENIQMENLEFKVKYSNIVEINDVINALDKMKTKLSESLTKQWDMEETRKEQIGA
LAHDIKTPLTIIRGNSELLKESDLNDEQTKFNDDILNEIKNMEFYIKSLIEITRSEKEPAIEKKQVNLIKFIDNIMKTGH
LISKNKNLSFKSRIKNIPEFIFIDEIALKRVINNIISNSVDYSLENGEILLTVDSDDKNIQFIIEDSGKGFTKEEISFAT
KQFFQGDKSRNSKNHYGMGLYIAEKIIEKHNGSIILENSKLLKGAKVILNLPNS
>Mature_454_residues
MERIKKQKSISKIFAIYVVLFCIVTIIVGVINLSLFMIGLNKGFILPANYYERRIENQRSEIAKAKDVKKLIPEECKYAV
YDLNGKVLQGNVSQDKAVGMWNIVKSNKISNGRYFYKIIQRYNGICVVEYRINVSFKNPTLRKYIKTPEVTFELLLLGLF
IFEILIFSRSFNKRLKKEIKILKDTTENIQMENLEFKVKYSNIVEINDVINALDKMKTKLSESLTKQWDMEETRKEQIGA
LAHDIKTPLTIIRGNSELLKESDLNDEQTKFNDDILNEIKNMEFYIKSLIEITRSEKEPAIEKKQVNLIKFIDNIMKTGH
LISKNKNLSFKSRIKNIPEFIFIDEIALKRVINNIISNSVDYSLENGEILLTVDSDDKNIQFIIEDSGKGFTKEEISFAT
KQFFQGDKSRNSKNHYGMGLYIAEKIIEKHNGSIILENSKLLKGAKVILNLPNS

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=288, Percent_Identity=22.5694444444444, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=236, Percent_Identity=22.8813559322034, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082128, Length=287, Percent_Identity=23.6933797909408, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=23.3050847457627, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=25.296442687747, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789808, Length=238, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=247, Percent_Identity=21.8623481781377, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788713, Length=269, Percent_Identity=23.4200743494424, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786783, Length=275, Percent_Identity=20.3636363636364, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994928, Length=218, Percent_Identity=24.3119266055046, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788279, Length=261, Percent_Identity=27.2030651340996, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790551, Length=310, Percent_Identity=20, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=279, Percent_Identity=22.5806451612903, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=288, Percent_Identity=25.6944444444444, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52530; Mature: 52530

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIKKQKSISKIFAIYVVLFCIVTIIVGVINLSLFMIGLNKGFILPANYYERRIENQRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHHHHH
EIAKAKDVKKLIPEECKYAVYDLNGKVLQGNVSQDKAVGMWNIVKSNKISNGRYFYKIIQ
HHHHHHHHHHHCHHHHCEEEEECCCEEEECCCCCCCCCCCEEHHHCCCCCCCHHHHHHHH
RYNGICVVEYRINVSFKNPTLRKYIKTPEVTFELLLLGLFIFEILIFSRSFNKRLKKEIK
HHCCEEEEEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDTTENIQMENLEFKVKYSNIVEINDVINALDKMKTKLSESLTKQWDMEETRKEQIGA
HHHCCCCCCEEECEEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAHDIKTPLTIIRGNSELLKESDLNDEQTKFNDDILNEIKNMEFYIKSLIEITRSEKEPA
HHHHHCCCEEEEECCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IEKKQVNLIKFIDNIMKTGHLISKNKNLSFKSRIKNIPEFIFIDEIALKRVINNIISNSV
HHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCC
DYSLENGEILLTVDSDDKNIQFIIEDSGKGFTKEEISFATKQFFQGDKSRNSKNHYGMGL
CEEECCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YIAEKIIEKHNGSIILENSKLLKGAKVILNLPNS
HHHHHHHHHCCCCEEEECCHHHCCCEEEEECCCC
>Mature Secondary Structure
MERIKKQKSISKIFAIYVVLFCIVTIIVGVINLSLFMIGLNKGFILPANYYERRIENQRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHHHHH
EIAKAKDVKKLIPEECKYAVYDLNGKVLQGNVSQDKAVGMWNIVKSNKISNGRYFYKIIQ
HHHHHHHHHHHCHHHHCEEEEECCCEEEECCCCCCCCCCCEEHHHCCCCCCCHHHHHHHH
RYNGICVVEYRINVSFKNPTLRKYIKTPEVTFELLLLGLFIFEILIFSRSFNKRLKKEIK
HHCCEEEEEEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDTTENIQMENLEFKVKYSNIVEINDVINALDKMKTKLSESLTKQWDMEETRKEQIGA
HHHCCCCCCEEECEEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAHDIKTPLTIIRGNSELLKESDLNDEQTKFNDDILNEIKNMEFYIKSLIEITRSEKEPA
HHHHHCCCEEEEECCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IEKKQVNLIKFIDNIMKTGHLISKNKNLSFKSRIKNIPEFIFIDEIALKRVINNIISNSV
HHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCC
DYSLENGEILLTVDSDDKNIQFIIEDSGKGFTKEEISFATKQFFQGDKSRNSKNHYGMGL
CEEECCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YIAEKIIEKHNGSIILENSKLLKGAKVILNLPNS
HHHHHHHHHCCCCEEEECCHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]