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The map label for this gene is spaR [H]

Identifier: 28210358

GI number: 28210358

Start: 646738

End: 647400

Strand: Direct

Name: spaR [H]

Synonym: CTC00628

Alternate gene names: 28210358

Gene position: 646738-647400 (Clockwise)

Preceding gene: 28210357

Following gene: 28210359

Centisome position: 23.1

GC content: 28.81

Gene sequence:

>663_bases
ATGGCATGCATTTTAGCTATAGATGATGAAGAAGGAATATTAACTATTATAAAAAGCGCCTTAGAAAAAGAAGGACATAA
TGTAACTACAGTTTCTAATCCCACATGTCTTTTAAAAGATAAATATTTAAAATATGATTTAATTTTACTTGATGTAATGA
TGCCAGAAATAGATGGTTTTTCTCTTTGTAAGGAAATACGTAATTTAGTTGATTGTCCAATAATATTTTTGACAGCTAAG
ACTATGGAACAAGATATTGTAATGGGATTAAGTTTGGGAGGGGACGATTATATATCTAAGCCTTTTGGCATTAGTGAACT
AAGAGCAAGAGTAGCGGCACATCTAAGACGTGAACATAGAGAAAAACAAAATGCTTTTTCTATATCCAATGTGAAATTTA
ATATAACTTCAAAAGAGGCATATTATAATGAAAAGTTAATACCTTTTACAAAAAGCGAGTATAATATATGTGAATATTTA
GCTATGAATCATGGTCAAGTATTTTCTAAAGATAGAATATATGAAAAAGTATATGGTACTTATGGAAATAGTGATACCAC
AGCAATTGTAGAACATATTAAAAACATACGAAATAAGTTAAAAAATATGGGGATAAATCCAATAGAAACAGTTTGGGGGA
TAGGGTATAAGTGGAAAGAATAA

Upstream 100 bases:

>100_bases
TTGGAATATGGTTTAACTTTTTTGAAGGTAGAAAAAGTGAATAATAAACAATGGGATTTGATGGTGATATAACGTATAAT
GGATTTGGGGAGGGATTAAA

Downstream 100 bases:

>100_bases
AGAAACAGAAAAGTATTTCTAAAATTTTTGCAATATATGTGGTTTTGTTTTGTATAGTTACAATTATTGTGGGAGTAATT
AATTTAAGTTTATTTATGAT

Product: subtilin biosynthesis regulatory protein spaR

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MACILAIDDEEGILTIIKSALEKEGHNVTTVSNPTCLLKDKYLKYDLILLDVMMPEIDGFSLCKEIRNLVDCPIIFLTAK
TMEQDIVMGLSLGGDDYISKPFGISELRARVAAHLRREHREKQNAFSISNVKFNITSKEAYYNEKLIPFTKSEYNICEYL
AMNHGQVFSKDRIYEKVYGTYGNSDTTAIVEHIKNIRNKLKNMGINPIETVWGIGYKWKE

Sequences:

>Translated_220_residues
MACILAIDDEEGILTIIKSALEKEGHNVTTVSNPTCLLKDKYLKYDLILLDVMMPEIDGFSLCKEIRNLVDCPIIFLTAK
TMEQDIVMGLSLGGDDYISKPFGISELRARVAAHLRREHREKQNAFSISNVKFNITSKEAYYNEKLIPFTKSEYNICEYL
AMNHGQVFSKDRIYEKVYGTYGNSDTTAIVEHIKNIRNKLKNMGINPIETVWGIGYKWKE
>Mature_219_residues
ACILAIDDEEGILTIIKSALEKEGHNVTTVSNPTCLLKDKYLKYDLILLDVMMPEIDGFSLCKEIRNLVDCPIIFLTAKT
MEQDIVMGLSLGGDDYISKPFGISELRARVAAHLRREHREKQNAFSISNVKFNITSKEAYYNEKLIPFTKSEYNICEYLA
MNHGQVFSKDRIYEKVYGTYGNSDTTAIVEHIKNIRNKLKNMGINPIETVWGIGYKWKE

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=34.5132743362832, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=31.4159292035398, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=35.3982300884956, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790860, Length=217, Percent_Identity=31.3364055299539, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=29.5964125560538, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=30.9090909090909, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786784, Length=176, Percent_Identity=34.0909090909091, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790552, Length=217, Percent_Identity=29.0322580645161, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=33.6363636363636, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787375, Length=218, Percent_Identity=31.651376146789, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786599, Length=221, Percent_Identity=28.0542986425339, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=25.8771929824561, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787229, Length=206, Percent_Identity=25.7281553398058, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=31.5789473684211, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=23.3050847457627, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=137, Percent_Identity=29.9270072992701, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25156; Mature: 25025

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACILAIDDEEGILTIIKSALEKEGHNVTTVSNPTCLLKDKYLKYDLILLDVMMPEIDGF
CEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHCCCCHH
SLCKEIRNLVDCPIIFLTAKTMEQDIVMGLSLGGDDYISKPFGISELRARVAAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHHHHHHEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHH
EKQNAFSISNVKFNITSKEAYYNEKLIPFTKSEYNICEYLAMNHGQVFSKDRIYEKVYGT
HHHCCEEECCEEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCC
YGNSDTTAIVEHIKNIRNKLKNMGINPIETVWGIGYKWKE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
ACILAIDDEEGILTIIKSALEKEGHNVTTVSNPTCLLKDKYLKYDLILLDVMMPEIDGF
EEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHCCCCHH
SLCKEIRNLVDCPIIFLTAKTMEQDIVMGLSLGGDDYISKPFGISELRARVAAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHHHHHHEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHH
EKQNAFSISNVKFNITSKEAYYNEKLIPFTKSEYNICEYLAMNHGQVFSKDRIYEKVYGT
HHHCCEEECCEEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCC
YGNSDTTAIVEHIKNIRNKLKNMGINPIETVWGIGYKWKE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]