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The map label for this gene is rihC [H]

Identifier: 28210313

GI number: 28210313

Start: 598188

End: 599120

Strand: Direct

Name: rihC [H]

Synonym: CTC00577

Alternate gene names: 28210313

Gene position: 598188-599120 (Clockwise)

Preceding gene: 28210312

Following gene: 28210314

Centisome position: 21.37

GC content: 28.94

Gene sequence:

>933_bases
GTGATTTTGAAAATGAATAAGAAAACTAAAATTATTTTAGATACAGATCCAGGAATTGATGATGCTGTAGCCATAGTAGC
CGCATTATTTAGTCCTAAAATTCAAGTGGAATTAATAACTACTGTATCTGGAAATGTAGATGTAGATAAAACAACAGAAA
ATGCACTGAAAATATTAGAATTTTTAAATAAAGATGTACCTATAGCAAAAGGTGCTAGAAAACCATTAATGAGAGAATTA
GATGATGCAAGTGATATACATGGAGATTCTGGATTAGATGGTTACAATTTTGGTAAAATTACAAGAAAAACAGATGAAAA
ACCCGCTATAGATAAGATGAGAGAAGTTATAATTAATTCAGAAGAGAAAATAACTTTAGTAGCAATAGGACCTTTAACTA
ATATTGCATTACTTTTAAGTTCATATCCAGAAGTTAAAGATAAAATAGACAAGTTAGTTATAATGGGGGGATCTGCATCT
AGAGGAAATAAAACACCTGCTGCGGAATTTAACATTTATGTTGACCCAGAAAGTGCTAATATAGTATTTAAATCTGGAAT
AAATATAGTAATGTGTGGGTTAGATATTACAAACAACGCTATATTTAAAGAAGAAGATATAAATTATATAAAAAATCTAA
ATAAAACAGGATATATGCTTTATTCATTATTTAAATGTTACAGAAGTGGAAGTTTTAAGAAGGGATTAAGAATGCATGAT
TTATGTGCAATAGCTTATTTAGATAAACCTGAAATATTTAAAGCGGTTTCTGCAAAGGTTGATATAGAAACCAAAGGAGA
ACATACATTAGGTGCCACAGTGGTAGACTTTGATAATTACTCTAATGGTAAGGCACAAGTGAAAGTATGCATGGAACTTG
ATGTAGAAGATTTAAAAAAGTGGTTCTTTAGTATATTGAATAAAGTAAATTAG

Upstream 100 bases:

>100_bases
AAATTTTCTATGATTTTTGGTGGAATAATTATTATAATATATATGATAACTAATTTACTAGTTAATAAACTAAAAGATGA
ATAACTAATTTACTTTAGGA

Downstream 100 bases:

>100_bases
GAAATAATTTAGGGACAGGGTTAAATATTTAAACTCTGTCCCTATATATTATTGTTTTTACCTTTTGATAATAGTATATT
AAAATATAGTTATATTAAAC

Product: ribonucleoside hydrolase RihC

Products: D-ribose; purine

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MILKMNKKTKIILDTDPGIDDAVAIVAALFSPKIQVELITTVSGNVDVDKTTENALKILEFLNKDVPIAKGARKPLMREL
DDASDIHGDSGLDGYNFGKITRKTDEKPAIDKMREVIINSEEKITLVAIGPLTNIALLLSSYPEVKDKIDKLVIMGGSAS
RGNKTPAAEFNIYVDPESANIVFKSGINIVMCGLDITNNAIFKEEDINYIKNLNKTGYMLYSLFKCYRSGSFKKGLRMHD
LCAIAYLDKPEIFKAVSAKVDIETKGEHTLGATVVDFDNYSNGKAQVKVCMELDVEDLKKWFFSILNKVN

Sequences:

>Translated_310_residues
MILKMNKKTKIILDTDPGIDDAVAIVAALFSPKIQVELITTVSGNVDVDKTTENALKILEFLNKDVPIAKGARKPLMREL
DDASDIHGDSGLDGYNFGKITRKTDEKPAIDKMREVIINSEEKITLVAIGPLTNIALLLSSYPEVKDKIDKLVIMGGSAS
RGNKTPAAEFNIYVDPESANIVFKSGINIVMCGLDITNNAIFKEEDINYIKNLNKTGYMLYSLFKCYRSGSFKKGLRMHD
LCAIAYLDKPEIFKAVSAKVDIETKGEHTLGATVVDFDNYSNGKAQVKVCMELDVEDLKKWFFSILNKVN
>Mature_310_residues
MILKMNKKTKIILDTDPGIDDAVAIVAALFSPKIQVELITTVSGNVDVDKTTENALKILEFLNKDVPIAKGARKPLMREL
DDASDIHGDSGLDGYNFGKITRKTDEKPAIDKMREVIINSEEKITLVAIGPLTNIALLLSSYPEVKDKIDKLVIMGGSAS
RGNKTPAAEFNIYVDPESANIVFKSGINIVMCGLDITNNAIFKEEDINYIKNLNKTGYMLYSLFKCYRSGSFKKGLRMHD
LCAIAYLDKPEIFKAVSAKVDIETKGEHTLGATVVDFDNYSNGKAQVKVCMELDVEDLKKWFFSILNKVN

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=299, Percent_Identity=54.180602006689, Blast_Score=320, Evalue=7e-89,
Organism=Escherichia coli, GI1786871, Length=293, Percent_Identity=45.0511945392491, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=36.1563517915309, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17565698, Length=340, Percent_Identity=29.1176470588235, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32565474, Length=229, Percent_Identity=31.4410480349345, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=33.5907335907336, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24583915, Length=190, Percent_Identity=33.1578947368421, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24641839, Length=196, Percent_Identity=31.6326530612245, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641837, Length=189, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34339; Mature: 34339

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKMNKKTKIILDTDPGIDDAVAIVAALFSPKIQVELITTVSGNVDVDKTTENALKILE
CEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
FLNKDVPIAKGARKPLMRELDDASDIHGDSGLDGYNFGKITRKTDEKPAIDKMREVIINS
HHCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
EEKITLVAIGPLTNIALLLSSYPEVKDKIDKLVIMGGSASRGNKTPAAEFNIYVDPESAN
CCCEEEEEECCHHHHHHHHHCCCHHHHHHCEEEEECCCCCCCCCCCCEEEEEEECCCCCC
IVFKSGINIVMCGLDITNNAIFKEEDINYIKNLNKTGYMLYSLFKCYRSGSFKKGLRMHD
EEEECCCEEEEECCEECCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LCAIAYLDKPEIFKAVSAKVDIETKGEHTLGATVVDFDNYSNGKAQVKVCMELDVEDLKK
EEEEEECCCHHHHHHHHCEEEEECCCCCEECEEEEECCCCCCCCEEEEEEEECCHHHHHH
WFFSILNKVN
HHHHHHHHCC
>Mature Secondary Structure
MILKMNKKTKIILDTDPGIDDAVAIVAALFSPKIQVELITTVSGNVDVDKTTENALKILE
CEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
FLNKDVPIAKGARKPLMRELDDASDIHGDSGLDGYNFGKITRKTDEKPAIDKMREVIINS
HHCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
EEKITLVAIGPLTNIALLLSSYPEVKDKIDKLVIMGGSASRGNKTPAAEFNIYVDPESAN
CCCEEEEEECCHHHHHHHHHCCCHHHHHHCEEEEECCCCCCCCCCCCEEEEEEECCCCCC
IVFKSGINIVMCGLDITNNAIFKEEDINYIKNLNKTGYMLYSLFKCYRSGSFKKGLRMHD
EEEECCCEEEEECCEECCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LCAIAYLDKPEIFKAVSAKVDIETKGEHTLGATVVDFDNYSNGKAQVKVCMELDVEDLKK
EEEEEECCCHHHHHHHHCEEEEECCCCCEECEEEEECCCCCCCCEEEEEEEECCHHHHHH
WFFSILNKVN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA